12-18 July 2008, Schroecken, Vorarlberg, Austria

Last update: 2008-07-10

• Download MiPsummer Programme [pdf 2.8 MB kB; Update: 2008-07-11]

    Lectures of the MiPsummer School on "Mitochondrial Respiratory Phyisology" provide a balance between an introduction into the basic concepts, advanced methodological and scientific approaches, and specific applications, with a focus on mitochondrial respiratory function linked to the general theme of MiP.

    For addresses of lecturers, see MiPsummer Participants.

   We may consider much of the program more as a workshop than just a lecture course, such that at any time of a lecture questions may be asked or comments be added, as long as we can adhere to the time schedule. 

Day 1: Mitochondrial respiratory capacity, coupling and respiratory control. Programme 1 - Abstracts 1

Day 2: Basic science and clinical reality 1. Programme 2 - Abstracts 2

Day 3: Oxygen kinetics of mitochondrial respiration and inhibition by nitric oxide. Programme 3 - Abstracts 3

Day 4: Mitochondrial membrane potential. Programme 4 - Abstracts 4

Day 5: Basic science and clinical reality 2. Programme 5 - Abstracts 5

Mitochondrial respiratory capacity and respiratory control. Abstracts Day 1

Chair:           Vilma Borutaite (LT), Steven C Hand (US)

1-01L   09:00-10:30  Erich Gnaiger (Innsbruck, AT) Mitochondrial respiratory control: Electron transport system, oxidative phosphorylation and leak – ETS, OXPHOS and LEAK. Poster 1-01L.

           10:30           Coffee

1-02L   11:00-12:30  Chuck Hoppel (Cleveland, USA) Substrates used in studies of OXPHOS. An application example: The effect of developmental age on mitochondrial function in human skeletal muscle.

           13:00           Lunch

Chair:           Dominique Votion (BE), Erich Gnaiger (AT)

1-03L   15:00-16:30  Rodrigue Rossignol (Bordeaux, FR) Ultrastructure of the mitochondrion and its bearing on function and bioenergetics.

                                      Flash presentations of posters:

1-04P   16:30-16:45  Estelle Hirzel (Basel, CH) Mitochondrial targeting in adipocytes using antioxidant tetrapeptides. Communicated by Vilma Borutaite.

1-05P   16:45-17:00  Leandro S. da Costa (Rio de Janeiro, BR) Mitochondrial dysfunction in neuroblastoma cells infected with sindbis virus. Communicated by Vilma Borutaite.

1-06P   17:00-17:15  René G Feichtinger (Salzburg, AT) Decrease of aerobic mitochondrial energy metabolism in paediatric solid tumors. Communicated by Steven C Hand.

1-07P   17:15-17:30  Franz A. Zimmermann (Salzburg, AT) Deficiency of complex I of the respiratory chain in oncocytic tumors. Communicated by Chuck Hoppel.

           17:30-18:45  Coffee and Posters

19:00           Dinner at Hotel Mohnenfluh

21:15           MiPboard Meeting: MiP2009 and beyond (Hotel Mohnenfluh)

Membrane potential, coupling, substrates and respiratory control

Abstracts Day 2

Chair:           Hélène Lemieux (AT), Guy C Brown (UK)

 

2-01L   09:00-10:30  Vilma Borutaite (Kaunas, LT) Mitochondrial membrane potential: why and how to measure.

 

           10:30           Coffee

 

2-02P   11:00-11:15  Patrick Subarsky (Innsbruck, AT) Regulation of oxidative phosphorylation in response to graded uncoupling towards the limit of electron transport capacity. Poster 2-02L Communicated by Guy C Brown.

2-03L   11:15-12:00  Dominique-Marie Votion (Liége, BE) Mitochondrial respiration in the equine muscle with high-resolution respirometry: Race horses and a rare myopathy.

2-04L   12:00-12:45  Erich Gnaiger (Innsbruck, AT) How are respiratory control ratios interpreted? – Concepts and applications. Poster 2-04L.

 

           13:00           Lunch

 

Chair:           Vilma Borutaite (LT), Chuck Hoppel (US)

 

                                      Flash presentations of posters:

 

2-05P   16:00-16:15  Olga Panasiuk (Kiev, UA) Different sensitivities of two mitochondrial subpopulations to calcium-induced injury. Communicated by Chuck Hoppel.

2-06P   16:15-16:30  José Lumini-Oliveira (Coimbra, PT) Effects of endurance treadmill training on skeletal muscle mitochondrial respiratory function of STZ-treated rats. Communicated by Chuck Hoppel.

2-07P   16:30-16:45  Liat Shachnai (Rehovot, IL) MTCH2/MIMP: A novel regulator of mitochondrial function. Communicated by Vilma Borutaite.

 

              16:45–17:45  Coffee and Posters

 

           17:45-18:45  Special interest groups

 

 

           19:00           Dinner at Hotel Mohnenfluh

 

21:15-22:15  Special interest groups

Gas interactions with mitochondria

Chair:           Dominique Votion (BE), Anthony Hickey (NZ)

 

3-01L   09:00-10:30   Guy C Brown (Cambridge, UK) Nitric oxide and other gas interactions with mitochondria.

 

           10:30-10:45  Questions and suggestions by participants

           10:45-11:45  Coffee and Posters / Special interest groups

 

                              In case of favourable weather conditions:

12:00           MiPsummer Walk to the cheese and wine reception at the Alpmuseum uf m Tannberg; refreshment in the lake Körebersee or at Hotel Körbersee.

 

Chair:           Francesca Scandurra (AT), Steven C Hand (US)

 

3-02L   17:15-18:00  Erich Gnaiger (Innsbruck, AT) Do we need a thermodynamics background in bioenergetics and mitochondrial physiology?

 

3-03D   18:00-18:45 Mario Fasching (Innsbruck, AT) Simultaneous high-resolution measurement of pH and  oxygen Flux with the Oxygraph-2k (instrument demo).

 

19:00           Dinner at Hotel Mohnenfluh.

Oxygen kinetics of mitochondrial respiration and spectrophotometry

Abstracts Day 4

Chair:           Vilma Borutaite (LT), Guy C Brown (UK)

 

4-01L   09:00-09:45   Francesca Scandurra (Innsbruck, AT) Cellular respiration under hypoxia: Mitochondrial oxygen kinetics at steady-state oxygen supply and aerobic-anaerobic transitions.

 

4-02D   09:45-11:00   Natascha Sommer and Thomas Derfuss (Giessen, DE) Simultaneous remission spectrophotometry and high-resolution respirometry. Respiratory control by oxygen and redox states of mitochondrial cytochromes in cells.

 

           11:00           Coffee and Posters

 

4-03L   11:45-12:45  Erich Gnaiger (Innsbruck, AT) Competitive and undcompetitive inhibition of cytochrome c oxidase by NO in living cells.

           13:00           Lunch

 

                              Heart and skeletal muscle mitochondria

 

Chair:           Natascha Sommer (DE), Steven C Hand (US)

 

4-04L   15:00-15:30  Chuck Hoppel (Cleveland, US) Ischemia-reperfusion injury in heart mitochondria.

4-05L   15:30-16:00  Hélène Lemieux (Innsbruck, AT) Mitochondrial dysfunction in pathologies of the human heart and comparison with mitochondrial respiratory control in the mouse heart.

 

                                      Flash presentations of posters:

 

4-06P   16:00-16:15  Julie H Rennison (Cleveland, US) High fat fed heart failure animals have enhanced mitochondrial function and acyl-CoA dehydrogenase activities. Communicated by Erich Gnaiger.

4-07P   16:15-16:30  Antonina V Pustovidko (Moscow, RU) Uncoupling effect of lauryl sulfate on mitochondria. Communicated by Erich Gnaiger.

4-08P                      Tímea Kurdiová (Bratislava, SK) Measurement of the mitochondrial respiration capacity in permeabilized muscle fibers from cold adapted mice. Communicated by Erich Gnaiger.

 

           16:30-17:45  Coffee and Posters

 

Chair:           Dominique Votion (BE), Charles Hoppel (US)

 

4-09L   17:45-18:45  Anthony J.R. Hickey (Auckland, NZ) Depressed endogenous mitochondrial respiration stimulates elevated cardiac superoxide production in spontaneously hypertensive rats.

 

           19:00           Dinner

 

21:00           MiPsociety Party

 

Mitochondrial molecular physiology

Abstracts Day 5

Chair:           Francesca Scandurra (AT), Guy C Brown (UK)

5-01L   09:00-10:30  Steven C Hand (Baton Rouge, US) Mitochondria and apoptosis: Energy-limited states and signaling for cell death.

           11:00           Coffee

5-02L   11:30-12:45  Charles Hoppel (Cleveland, US) Integrated mitochondrial function and the clinical utility of polarographic analysis.

           13:00           Lunch

 

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5-03L   15:00-16:00  Steven C Hand (Baton Rouge, US) Mitochondrial targeting of protein import.

 

                                      Flash presentations of posters:

 

5-04P   16:00-16:15  Ana Paula P. da Silva (Rio de Janeiro, BR) Differential inhibition of energy-producing pathways of hepg-2 cells by 3-bromopyruvate. Communicated by Steven C Hand.

5-05P   16:15-16:30  Elisa Sottotetti (Bologna, IT) PKA stimulation protects transformed cells from apoptosis induced by glucose depletion, by re-activating mitochondrial activity. Communicated by Erich Gnaiger.

5-06P   16:30-16:45  Alexandra Latini (Florianopolis, BR) Seleno compounds prevents the brain energy impairment induced by methylmercury poisoning in adult mice. Communicated by Guy C Brown.

5-07P   16:45-17:00  Praturi Gopalakrishna (Hyderabad, IN) Periodontitis: Role of oxidative phosphorylation. Communicated by Guy C Brown.

 

           17:00-17:45 Coffee and Posters

 

          17:45-18:30 MiPsummer Feedback

           18:30           Break

 

           19:00           MiPsummer Dinner at Hotel Mohnenfluh

 

5-08S    21:15           MiPsummer Special Lecture (in the lecture hall)

                              Guy C Brown (Cambridge, UK) Mitochondria and the future of death.

                                      Guy Brown is the author of ‘The Living End. The future of death, aging and immortality’. Macmillan (2007).