MiP2005: Session 1
Mitochondrial Physiology Network 10.9: 22 (2005) - download pdf
Effect of coenzyme Q10 supplementation on heart and liver mitochondrial function during exercise.
Li Wen1, JL Nie1,3, Y Zhang1, SS Liu 2
1Tianjin Research Institute of Sports Medicine; Dept. Health and Exercise Science, Tianjin Inst. Physical Education, Tianjin 300381, China; 2National Key Lab. of Biomembrane and Membrane Biotechnology Inst. Zoology, Chinese Academy of Sciences, Beijing 100080, China; 3Coll. Physical Education, Liaoning Normal University, China. - firstname.lastname@example.org
Several studies have showed the effect of supplement of coenzyme Q (CoQ) on ability of exercise, but the effect of supplement of coenzyme on mitochondria function in exercise is unclear [1,2]. This investigation will research the effect of Coenzyme Q10 on mitochondrial function during exercise from the point of view of biochemistry and bioenergetics.
SD rats were divided into four groups: normal control group (NC); supplement Q control group (QC); normal exercise group (NE); supplement Q and exercise group (QE). Exercise group rats were forced to run on the treadmill and were sacrificed at 120 min after exercise. The parameters were following: (1) Mitochondria CoQ concentrations in heart and liver were analyzed by HPLC . (2) The activities of syntheses were measured with luciferase-luciferin. (3) The initial rates of proton ejection and electron transferring were measured with potassium ferricyanide as an electron acceptor. (4) ROS generation rate were determined by fluorometric probe. (5) MDA concentrations were measured in myocardium, livers and serum.
(1) In coenzyme Q10-treated rats, the coenzyme Q10 level of mitochondria increased significantly in myocardium and liver; the coenzyme Q9 level of mitochondria increased significantly in myocardium also. (2) Comparing with normal control group, QC and QE group H+/2e and H+-ATPase synthesis activity increased significantly in myocardium and liver. Comparing with NE group, QE group H+/2e increased significantly in myocardium. The results indicated that supplement of exogenous coenzyme Q improves the coupling of oxidative phosphorylation and increases the rate of mitochondria ATP synthesis activity. (3) Supplementing CoQ increased significantly ROS generation in rats in stage of exercise. The level of MDA of mitochondria increased significantly after treat with coenzyme Q10 in myocardium and livers. There are no differences in the level of MDA in serum in each group.
Supplement of exogenous coenzyme Q10 can increase the level of CoQ in mitochondria and the efficiency of energy transduction and the rate of ATP synthesis in mitochondria. At the same time, it increases production of free radicals and level of LPO.
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3. Johanna KL, Kishotchandra G, Lester P (1986) Simultaneous determination of tocopherols, ubiquinols, and ubiquinones in blood, plasma, tissue homogenates, and subcellular fraction. Anal. Biochem. 157: 106-116.