https://wiki.oroboros.at/api.php?action=feedcontributions&user=Bitschnau+Barbara&feedformat=atomBioblast - User contributions [en]2024-03-28T18:35:33ZUser contributionsMediaWiki 1.36.1https://wiki.oroboros.at/index.php?title=Went_Nora&diff=135385Went Nora2017-05-11T06:34:05Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{Person<br />
|lastname=Went<br />
|firstname=Nora<br />
|institution=::::::::::::::::::::[[File:Nora Went.jpg|150px|right|Nora Went]]<br />
'''OROBOROS INSTRUMENTS'''<br />
:: Mitochondria and cell research<br />
<br />
Nora Went was part of the [[OROBOROS_Contact |OROBOROS]] team until June 2015.<br />
<br />
|area code=6020<br />
|city=Innsbruck<br />
|country=Austria<br />
}}<br />
{{Labelingperson}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Lamberti_G&diff=135384Lamberti G2017-05-11T06:18:44Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{Person<br />
|lastname=Lamberti<br />
|firstname=Giorgia<br />
|title=PhD.<br />
|institution=:::::::::::::::::::[[File:LambertiG.JPG|right|150px|Giorgia Lamberti]]<br />
'''OROBOROS INSTRUMENTS''' <br />
:: Mitochondria and cell research<br />
<br />
Giorgia Lamberti was part of the [[OROBOROS_Contact |OROBOROS]] team until August 2016.<br />
<br />
|address=Schoepfstr. 18<br />
|area code=6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress=<br />
|weblink=<br />
}}<br />
{{Labelingperson<br />
|field of research=Basic<br />
}}<br />
<br />
<br />
== Participated at ==<br />
* [[MitoFit Science Camp 2016 Kuehtai AT]], [[IOC112]]<br />
* [[MiPNet21.01 IOC109 Schroecken AT|IOC109 Schroecken]]<br />
* [[MitoFit Workshop Blood Cells 2016-01-08|MitoFit Workshop Blood Cells]]<br />
* [[MiPNet20.10 IOC106 Schroecken|IOC106 Schroecken]]</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Gasser_J&diff=135383Gasser J2017-05-11T06:18:20Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{Person<br />
|lastname=Gasser<br />
|firstname=Juliane<br />
|institution=OROBOROS INSTRUMENTS <br />
<br />
high-resolution respirometry<br />
<br />
Juliane Gasser was part of the [[OROBOROS_Contact |OROBOROS]] team until September 2015.<br />
|address=Schöpfstr. 18<br />
|area code=A-6020<br />
|city=Innsbruck<br />
|country=Austria<br />
}}<br />
{{Labelingperson}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Bufe_A&diff=135382Bufe A2017-05-11T06:14:52Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{EAGLE<br />
|COST= Member<br />
|COST WG1= WG1 <br />
|COST WG4= WG4}}<br />
<br />
{{Person<br />
|lastname=Bufe<br />
|firstname=Anja<br />
|title=MSc.<br />
|institution=:::::::::::::::[[File:BufeA.JPG|right|150px|Anja Bufe]] <br />
'''OROBOROS INSTRUMENTS'''<br />
:: Mitochondria and cell research<br />
'''PhD. Student'''w<br />
:* [[TRACT|TRACT]]<br />
:* '''Title of dissertation:''' Metabolic profiles in normal, dysplastic and cancerous oral cells<br />
<br />
Anja Bufe joined [[OROBOROS_Contact |OROBOROS]] in March 2017.<br />
<br />
<br />
|address=Schöpfstrasse 18<br />
|area code=A-6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress=anja.bufe@oroboros.at<br />
|weblink=http://wiki.oroboros.at/index.php/TRACT<br />
}}<br />
{{Labelingperson<br />
|field of research=Basic<br />
|topics=[[High-resolution respirometry]]<br />
}}<br />
<br />
[[Image:TRACT logo.png|right|170px|link=http://wiki.oroboros.at/index.php/TRACT]]<br />
[[Image:Marie Curie.jpg|right|170px|link=https://ec.europa.eu/research/mariecurieactions/]]<br />
<br />
== TRACT ==<br />
<br />
::::* '''2017-03-01''' Anja started her Marie Skłodowska-Curie PhD Fellowship with [[OROBOROS INSTRUMENTS]] at the [https://www.i-med.ac.at/ Medical University Innsbruck], supported by [http://www.qub.ac.uk/sites/TraininginCancerMechanismsandTherapeutics/ TRACT] - Training in Cancer Mechanisms and Therapeutics.<br />
::::* '''2017-03-27''' Anja presented her project at the Kick-off meeting in Dublin for the first time in front of the TRACT core members and other TRACT PhD students<br />
<br />
<br />
== Participated at ==<br />
::::* [[TRACT Kick-off meeting Dublin IE| TRACT Kick-off meeting]]<br />
::::* [[MITOEAGLE_Barcelona_2017]]<br />
::::* [[MiPNet22.03 IOC119 Innsbruck AT| IOC119 Innsbruck AT]]</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Oroboros_Laboratory:_visiting_scientists&diff=135381Oroboros Laboratory: visiting scientists2017-05-11T06:09:39Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{OROBOROS header page name}}<br />
:::: <big>'''Following to the concept of open innovation and [[Gentle Science]], the [[OROBOROS MitoFit Laboratory]] welcomes visiting researchers from all over the world.'''</big><br />
<br />
[[File:OROBOROS MitoFit-Lab.jpg|left|240px|link=OROBOROS MitoFit Laboratory |MitoFit Laboratory]]<br />
[[Image:Logo MitoFit_s.jpg|right|100px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]]<br />
[[Image:O2k-Network.png|right|100px|link=O2k-Network|O2k-Network]]<br />
__TOC__<br />
::::» [[OROROBOS Science Scholarship]]s<br />
<br />
== 2017 ==<br />
{| class="wikitable" border="1"<br />
|-<br />
! From<br />
! Until<br />
! Visiting scientist<br />
! Country<br />
! [[O2k-Network |O2k-Network lab]] <br />
! Scholarship<br />
! Topic<br />
<br />
|-<br />
| Jan-15<br />
| Jul-15<br />
| [[Velika B|Beata Velika]]<br />
| SK<br />
| <br />
| [http://www.aktion.saia.sk/de Postdoc Scholarship Action Austria – Slovakia]<br />
| Human blood cells as study model of mitochondrial respiration and H2O2 production <br />
<br />
|-<br />
| Mar-01<br />
| Apr-25<br />
| [[Sobotka O|Ondrej Sobotka]]<br />
| CZ<br />
| [[CZ Hradec Kralove Cervinkova Z]]<br />
| [https://www.oead.at/index.php?id=2300&L=1 Postdoc Scholarship Action Austria – Czech Republic]<br />
| Methodological optimalization of continuous reactive oxygen species (ROS) measurement using fluorescent dye Amplex Ultra Red and O2k-fluorometry system<br />
<br />
|-<br />
| Feb-20<br />
| May-19<br />
|[[Permer M|Manuel Permer]]<br />
|AT <br />
|<br />
|<br />
|Respirometric characterization of cryopreserved HEK293T cells<br />
<br />
<br />
|-<br />
| Jun-01<br />
| Aug<br />
|[[Wohlfarter Y|Yvonne Wohlfarter]] (Internship)<br />
|AT<br />
| <br />
|<br />
|<br />
<br />
|-<br />
| Jun-25<br />
| Aug-25<br />
|[[Ghanim M|Magda Ghanim]]<br />
|IE<br />
|[[IE Dublin Porter RK]]<br />
|[[TRACT]]<br />
|HAMLET derivatives as a pre-operative therapy in oesophageal cancer<br />
<br />
|-<br />
| Jun-26<br />
| Aug-25<br />
|[[Karavyraki M|Marilena Karavyraki]]<br />
|IE<br />
|[[IE Dublin Porter RK]]<br />
|[[TRACT]]<br />
|Mitochondrial function linked to metabolic differences in normal, dysplastic and cancerous oral cells<br />
<br />
|-<br />
| Jul<br />
| Aug<br />
|[[Matic I|Ines Matic]] (Internship)<br />
|AT<br />
| <br />
|<br />
|<br />
|}<br />
<br />
<br />
== 2016 ==<br />
{| class="wikitable" border="1"<br />
|-<br />
! From<br />
! Until<br />
! Visiting scientist<br />
! Country<br />
! [[O2k-Network |O2k-Network lab]] <br />
! Scholarship<br />
! Topic<br />
<br />
|-<br />
| Feb-01<br />
| Feb-28<br />
| [[Bhattarai N|Nisha Bhattarai]]<br />
| US<br />
| [[US TX Galveston Porter C]]<br />
| [[OROROBOS Science Scholarship]]<br />
| mt-membrane potential<br />
<br />
|-<br />
| Feb-01<br />
| Mar-30<br />
| [[Chang SC|Chang Shao-Chiang]] <br />
| TW<br />
| [[TW Taoyuan Wang JS]]<br />
|<br />
| [[O2k-Fluorometer]]<br />
<br />
|-<br />
| Feb-01<br />
| Feb-07<br />
| [[Chou Chen-Hsien|Chen-Hsien Chou ]] <br />
| TW<br />
| [[TW Taoyuan Wang JS]]<br />
|<br />
| [[O2k-Fluorometer]]<br />
<br />
|-<br />
| Feb-15<br />
| Mar-15<br />
| [[Garcia-Souza LF|Luiz Felipe Garcia-Souza]]<br />
| BR<br />
| [[BR Rio de Janeiro Oliveira MF]]<br />
| [[MitoFit]]<br />
| [[MitoFit-Bloodcells]]<br />
<br />
|-<br />
| Mar-01<br />
| Jun-10<br />
| [[Sobotka O|Ondrej Sobotka]]<br />
| CZ<br />
| [[CZ Hradec Kralove Cervinkova Z]]<br />
| [http://www.cuni.cz/UKEN-158.html Charles University mobility fund]<br />
| [[Amplex red |ROS production]]<br />
<br />
|-<br />
| Mar-01<br />
| Apr-01<br />
| [[Volska K| Volska Kristine]] <br />
| LV<br />
| [[LV Riga Makrecka-Kuka M]]<br />
|<br />
| [[O2k-TPP+ ISE-Module]]<br />
<br />
|-<br />
| Apr-03<br />
| Jun-10<br />
| [[Jha RK| Jha Rajan Kumar]]<br />
| IN<br />
| [[IN Hyderabad Thangaraj K]]<br />
| [[OROROBOS Science Scholarship]]<br />
| [[MiPNet21.01_IOC_Schroecken_AT |O2k-Workshop]], [[O2k-Core |HRR]]<br />
<br />
|-<br />
| Jun-15<br />
| Jun-24<br />
| [[Jang D|David Jang]] <br />
| US<br />
| [[US PA Philadelphia Jang DH]]<br />
| [http://www.sfrbm.org/sections/education/fellowships SFRBM Mini-Fellowship Grant]<br />
| [[Amplex red |ROS production]], [[O2k-Publications:_Blood_cells |PBMCs]]<br />
<br />
|-<br />
| Nov-21<br />
| Nov-28<br />
| [[Meszaros A|Andras Meszaros]] <br />
| HU<br />
| [[HU Szeged Boros M]]<br />
| COST Action MITOEAGLE CA15203 [http://www.mitoglobal.org/index.php/Short-Term_Scientific_Missions_MITOEAGLE STSM Grant Period 1]<br />
| Harmonizing protocols of high resolution respirometry and fluorometry<br />
<br />
|-<br />
| Nov-21<br />
| Nov-28<br />
| [[Komlodi T|Timea Komlodi]] <br />
| HU<br />
| [[HU Budapest Tretter L]]<br />
| COST Action MITOEAGLE CA15203 [http://www.mitoglobal.org/index.php/Short-Term_Scientific_Missions_MITOEAGLE STSM Grant Period 1]<br />
| Standardization of protocols in mitochondria respirometry and fluorometry <br />
<br />
|-<br />
| Dec-12<br />
| Dec-16<br />
| [[Dymkowska D|Dorota Dymkowska]] <br />
| PL<br />
| [[PL Warsaw Zablocki K]]<br />
| COST Action MITOEAGLE CA15203 [http://www.mitoglobal.org/index.php/Short-Term_Scientific_Missions_MITOEAGLE STSM Grant Period 1]<br />
| Training in the MitoFit Laboratory <br />
<br />
|}<br />
----<br />
<br /><br />
::: <big>'''March 2017'''</big><br />
<br />
[[File:Sobotka O.JPG|left|400px|thumb|'''Ondrej Sobotka''']]<br />
:::: [[Sobotka O|Ondrej Sobotka]] (Czech Republic). Visiting scientist within the scholarship of the [http://www.dzs.cz/de/aktion-osterreich-tschechische-republik/stipendien/ AKTION Österreich – Tschechische Republik]. Mar 01 - Apr 25.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[CZ Hradec Kralove Cervinkova Z]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
::: <big>'''January 2017'''</big><br />
<br />
[[File:VelikaB.JPG|left|400px|thumb|'''Beata Velika''']]<br />
:::: [[Velika B|Beata Velika]] (Slovakia): Visiting scientist within the framework of the [http://www.aktion.saia.sk/de Postdoc Scholarship Action Austria]. Jan 15 - Jul 15. <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /> <br /><br /><br />
<br />
::: <big>'''December 2016'''</big><br />
<br />
:::: [[Dymkowska D|Dorota Dymkowska D]] (Hungary): Dec 12 - 16.<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[PL Warsaw Zablocki K]] <br />
<br /><br /><br />
<br />
::: <big>'''November 2016'''</big><br />
<br />
[[File:KomlodiTimea.JPG|left|400px|thumb|'''Timea Komlòdi''']]<br />
:::: [[Komlodi T|Timea Komlodi]] (Hungary): Nov 21 - 28.<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[HU Budapest Tretter L]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:Meszaros_Andras.JPG|left|400px|thumb|'''András Mészáros''']]<br />
:::: [[Meszaros A|Andras Meszaros]] (Hungary): Nov 21 - 28.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[HU Budapest Tretter L]] <br />
<br />
<br />
<br /><br /> <br /><br /> <br /> <br /><br /> <br /><br /> <br /> <br /><br /> <br /><br /><br />
::: <big>'''June 2016'''</big><br />
<br />
[[File:JangD.JPG|left|400px|thumb|'''David Jang''']]<br />
:::: [[Jang D|David Jang]] (USA): Visiting scientist within the framework of the [http://www.sfrbm.org/sections/education/fellowships SFRBM Mini-Fellowship Grant]. Jun 15 - Jul 15.<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] US PA Philadelphia Jang DH <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
::: <big>'''April 2016'''</big><br />
<br />
[[File:JhaRK.JPG|left|400px|thumb|'''Rajan Kumar Jha''']]<br />
:::: [[Jha RK|Rajan Kumar Jha]] (India): Apr 03 - Jun 10.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[IN Hyderabad Thangaraj K]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
::: <big>'''March 2016'''</big><br />
[[File:Volska K.JPG|left|400px|thumb|'''Kristine Volska''']]<br />
<br />
:::: [[Volska K| Kristine Volska]] (Latvia): Mar 01 - Apr 01.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[LV Riga Makrecka-Kuka M]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
<br />
[[File:Sobotka O.JPG|left|400px|thumb|'''Ondrej Sobotka''']]<br />
<br />
:::: [[Sobotka O|Ondrej Sobotka]] (Czech Republic): Visiting scientist within the framework of the [http://www.cuni.cz/UKEN-158.html Mobility fund of the Charles University]. Mar 01 - Jun 10.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[CZ Hradec Kralove Cervinkova Z]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
::: <big>'''February 2016'''</big><br />
[[File:Bhattarai N.JPG|left|400px|thumb|'''Nisha Bhattarai preparing an experiment''']]<br />
<br />
<br />
:::: [[Bhattarai N|Nisha Bhattarai]] (USA): Feb 01 - Feb 28.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[US TX Galveston Porter C]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
[[File:Chiang SC.JPG|left|400px|thumb|'''Shao-Chiang Chang is highly experienced with the O2k''']]<br />
<br />
:::: [[Chang SC|Shao-Chiang Chang]] (Taiwan): Feb 01 - Mar 30.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[TW Taoyuan Wang JS]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:Chou CH.JPG|left|400px|thumb|'''Chen-Hsien Chou generating data on HRR and proton flux''']]<br />
<br />
:::: [[Chou Chen-Hsien|Chen-Hsien Chou]] (Taiwan): Feb 01 - Feb 07.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[TW Taoyuan Wang JS]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
[[File:Garcia-Souza LF.JPG|left|400px|thumb|'''Luiz Felipe Garcia-Souza''']]<br />
<br />
:::: [[Garcia-Souza LF|Luiz Felipe Garcia-Souza]] (Brazil): Feb 15 - Mar 15.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[BR Rio de Janeiro Oliveira MF]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
== 2015 ==<br />
<br />
[[File:Bezuidenhout N.jpg|left|400px|thumb|'''Nicole Bezuidenhout tenderly preparing the O2k for an experiment]]<br />
::: <big>'''October 2015'''</big><br />
:::: [[Bezuidenhout N|Nicole Bezuidenhout]] (South Africa): Oct 05 - Nov 30.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[ZA Cape Town Ojuka EO]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:Spinazzi Marco.JPG|left|400px|thumb|'''Marko Spinazzi (right) analyzing data with Gerhard Krumschnabel]]<br />
:::: [[Spinazzi M|Marco Spinazzi]] (Belgium): Oct 21-22.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[BE Leuven Spinazzi M]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:Molina Tyrrell.JPG|left|400px|thumb|'''Daniel Tyrrell, Anthony Molina and Erich Gnaiger (f.l.t.r.) in front of the OROBOROS main office]]<br />
:::: [[Molina AJA| Anthony Molina]] and [[Tyrrell D| Daniel Tyrrell]] (USA): Oct 04-06.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[US NC Winston-Salem Molina AJA]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
[[File:McManus MJ.jpg |left|400px|thumb|'''[[McManus MJ| Meagan McManus]] preparing tissue for an experiment''']]<br />
::: <big>'''September 2015'''</big><br />
[[File:Meagan McManus.png|60px]] [[McManus MJ| Meagan J McManus]] (USA): Sep 14 - Oct 11.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[US PA Philadelphia Wallace DC]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
::::* Maryke Schoonen and Michelle Mereis (South Africa): Sep 28, University of Potchefstroom <br />
<br />
<br />
[[File:SiewieraK.JPG |left|400px|thumb|'''[[Doerrier C| Carolina Doerrier]] (left) and Karolina Siewiera experimenting on blood cells''']]<br />
::: <big>'''July 2015'''</big><br />
:::: [[Siewiera K| Karolina Siewiera]] (Poland): Visiting scientist within the framework of the [http://ec.europa.eu/programmes/erasmus-plus/discover/guide/index_en.htm ERASMUS]-programme. Jul 1 - Sep 30.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[PL Lodz Watala C]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
::: <big>'''March 2015'''</big><br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Calabria E |Elisa Calabria]] (Italy): Mar 09 to Aug. - O2k-Network Lab: [[IT Verona Capelli C]]<br />
<br />
<br />
::: <big>'''February 2015'''</big><br />
<br />
:::: [[File:SumbalovaZ.JPG|60px]] [[Sumbalova Z| Zuzana Sumbalova]] (Slovak Republic): High-resolution measurement of mitochondrial membrane potential and respiration, combination of potentiometric (TPP+) and fluorometric (TMRM, safranin) methods; in the framework of [http://www.aktion.saia.sk/de Aktion Österreich – Slowakei]. Feb 01 to Jun 30. - O2k-Network Lab: [[SK Bratislava Sumbalova Z]]<br />
<br />
<br />
== 2014 ==<br />
<br />
::: <big>'''September 2014'''</big><br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Sumbalova Z| Zuzana Sumbalova]] (Slovak Republic): Sep 08 to Oct 04, [http://ec.europa.eu/programmes/erasmus-plus/discover/guide/index_en.htm ERASMUS] scholarship - MiP2014, IOC95 and [[malate]] project. - O2k-Network Lab: [[SK Bratislava Sumbalova Z]]<br />
<br />
<br />
[[File:Lab Kumphune Sarawut 1.JPG |left|400px|thumb|'''Sarawut Kumphune researching on mitochondrial function''']]<br />
::: <big>'''August 2014'''</big><br />
:::: [[File:Sarawut Kumphune.JPG|60px]] [[Kumphune S |Assist. Prof. Dr. Sarawut Kumphune]] (Thailand): Visiting scientist within the framework of the [http://www.asea-uninet.org/ ASEA-UNINET Programme]. Aug 2014 (one month).<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[TH Phitsanulok Kumphune S]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:Lab_MakreckaM_DavidikovaS.JPG|left|400px|thumb|'''Marina Makrecka (right) and Sarka Davidikova exploring the world of the O2k''']]<br />
:::: [[File:MakreckaM.jpg|60px]] [[Makrecka-Kuka M| Marina Makrecka-Kuka]] (Latvia): Visiting scientist within the framework of [http://ec.europa.eu/research/fp7/index_en.cfm/ FP7]. Aug-Sep 2014 (two months).<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[LV Riga Makrecka-Kuka M]]<br />
<br />
<br />
[[File:Sarka Davidikova.JPG|60px]] [[Davidikova S |Sarka Davidikova]] (Czech Republic): Visiting scientist within the framework of the [http://ec.europa.eu/programmes/erasmus-plus/discover/guide/index_en.htm ERASMUS]-programme. Aug-Nov 2014 (three months).<br />
<br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
::: <big>'''July 2014'''</big><br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Malacrida S| Sandro Malacrida]] (EURAC, Bolzano, IT), [[Pichler I |Irene Pichler]] (EURAC, Bolzano, IT), Alessandra Zenon (EURAC, Bolzano, IT) visited from Bolzano for a test experiment with their cultured cells. - O2k-Network Lab: [[IT Bolzano Pichler I]]<br />
<br />
<br />
[[File:MitoLab 1.JPG|left|400px|thumb|'''[[Krumschnabel G| Gerhard Krumschnabel]], Erika Nydlova and Martina Vrbova in the OROBOROS MitoLab''']]<br />
::: <big>'''May 2014'''</big><br />
:::: [[Vrbova Martina | Martina Vrbova]] (Czech Republic): May 2014.<br />
<br />
:::: [[Nydlova Erika | Erika Nydlova]] (Czech Republic): May 2014.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[CZ Pardubice Rousar T]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:MitoLab 03.JPG|400px|left|thumb|'''Lindsay Benage performing an experiment''']]<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Benage Lindsay | Lindsay Benage]] (USA): May 2014. - O2k-Network Lab: [[US CO Fort Collins Chicco AJ]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
== 2013 ==<br />
<br />
::: <big>'''July 2013'''</big><br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Krautschneider M| Martin Krautschneider]] (Austria): Jul 01 to Aug 23. - O2k-Network Lab: [[AT Innsbruck Burtscher M]]<br />
<br />
<br />
::: <big>'''June 2013'''</big><br />
:::: [[File:GomezA.jpg|60px]] [[Gomez Rodriguez A| Gomez Rodriguez Alexia]] (Spain, Complutense University of Madrid ): Jun 04 to Jul 31<br />
<br />
<br />
::: <big>'''April 2013'''</big><br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Khalifa ARM| Abdel Rahman Medhat Khalifa]] (Egypt): Apr 15 to Jun 10, O2k-Network Lab: [[ET Giza Ali SS]]<br />
<br />
:::: [[File:Islam.jpg|60px]] [[Kotb IA| Islam Abo El Yazeed Kotb]] (Egypt): Apr 15 to Jun 10, O2k-Network Lab: [[ET Giza Ali SS]]<br />
<br />
<br />
== 2012 ==<br />
<br />
::: <big>'''October 2012'''</big><br />
:::: [[File:Gergö.jpg|60px]] [[Horvath Gergoe| Gergoe Horvath]] (Hungary): Visiting scientist within the framework of the [http://ec.europa.eu/programmes/erasmus-plus/discover/guide/index_en.htm| ERASMUS]-programme. Oct 2012 to Jan 2013, O2k-Network Lab: [[HU Budapest Tretter L]]</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=MiPschool_Obergurgl_2017&diff=135378MiPschool Obergurgl 20172017-05-10T13:58:33Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MiP header page name}}<br />
{{Publication<br />
|title=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]]<br />
[[Image:MiPsocietyLOGO.JPG|right|120px|link=http://www.mitophysiology.org|MiPsociety]]<br />
'''Obergurgl AT''', 2017 Jul 23-30. '''10<sup>th</sup> MiP''school'' 2017 MITOEAGLE and MITOEAGLE Workshop WG1-4.<br />
|info=<br />
|authors=Mitochondrial Physiology Society<br />
|year=2017-07-23<br />
|journal=MitoGlobal<br />
|abstract='''2017 Jul 23-30, Obergurgl, AT.''' <br />
|mipnetlab=<br />
}}<br />
[[File:MUI Logo rz rgb.jpg|150px|right|link=https://www.i-med.ac.at |Medical University Innsbruck]]<br />
[[File:Logo_age_reg.jpg|150px|right|link=https://www.uibk.ac.at/forschung/doktoratskollegs/index.html.de| DK Ageing and Regeneration]]<br />
[[File:Logo_Univ_Innsbruck.jpg|100px|right|link=https://www.uibk.ac.at |University of Innsbruck]]<br />
<br />
<br />
__TOC__<br />
== Date, venue, organization ==<br />
::::* 2017 July 23-30<br />
:::::* 2017 July 23-27 Training School jointly organized by the [[Mitochondrial Physiology Society]] and [[MC_MITOEAGLE_e-VOTE_02#Topic_.282.29_Training_School_in_Obergurgl.2C_Tyrol.2C_AT_in_July_2017 |COST Action MITOEAGLE]]<br />
:::::* 2017 July 27-30 MITOEAGLE Workshop: [[MITOEAGLE_Working_Groups|WG1-4]]<br />
::::* [http://www.uz-obergurgl.at University Centre Obergurgl], Tyrol, Austria<br />
<br />
<br />
== Programme ==<br />
<br />
=== Programme structure ===<br />
::::* '''2017-07-23, Day 1:''' Arrival<br />
::::* '''2017-07-24 to 26, Day 2-4: Training school MiP''society'' and MITOEAGLE''' <br />
::::** Day 2-3: Intoductory lectures on the mitochondrial electron transfer system (ETS), coupling of electron transfer to proton translocation and phosphorylation (OXPHOS), mitochondrial pathways, respiratory protocols for diagnosis of mitochondrial function, mitochondrial respiratory control in health and disease.<br />
::::** Day 4: Scientific lectures and student presentations<br />
::::* '''2017-07-27, Day 5:''' Departure Training school, arrival MITOEAGLE workshop, relaxing day for overall-participants<br />
::::* '''2017-07-28 to 29, Day 6-7: MITOEAGLE Workshop: WG1-4'''<br />
::::** '''WG1: Harmonization of nomenclature on mitochondrial respiratory states and control parameters:''' Status of the ''consensus paper'' on a consistent terminology on mitochondrial physiology and bioenergetics; recommendations for the use of a common terminology in mitochondrial physiological research as a milestone towards unification of concepts and nomenclature.<br />
::::** '''WG1: Respirometric reference protocols:''' (i) Standard format for presenting substrate-uncoupler-inhibitor titration (SUIT) protocols for a ‘library of protocols’ applied in mitochondrial respiratory physiology; (ii) criteria for selecting and recommending reference protocols; (iii) documentation of the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating SUIT protocols (compare: Maelstrom Research program).<br />
::::** '''WG2-4: Data repositories'''<br />
::::* '''2017-07-30, Day 8:''' Departure MITOEAGLE<br />
<br />
=== Preliminary programme MiP''school'' (Jul 23-27) ===<br />
<br />
:::: This MiP''school'' will focus on basic concepts on mitochondrial respiratory states and applications of substrate-uncoupler-inhibitor titration (SUIT) protocols. <br />
<br />
****: '''2017-07-24'''<br />
:::: '''Session A''' (morning) '''Introduction: coupling control in oxidative phosphorylation'''<br />
::::::* A1. The electron transfer system – mitochondrial pathways from fuel substrates to oxygen.<br />
::::::* A2. Coupling of the phosphorylation system to electron transfer and respiratory coupling control.<br />
::::::* A3. From Einstein's diffusion equation to Mitchell’s chemiosmotic equation: rates and states in the bioenergetics of oxidative phosphorylation.<br />
::::::* A4. Measurement of coupling in intact cells and mitochondrial preparations: biochemical coupling efficiency and ATP/O2 ratios.<br />
<br />
:::: '''Session B''' (afternoon) '''Posters and selected abstracts'''<br />
::::::* B1. Poster session.<br />
::::::* B2: Selected abstracts: measurement of coupling control in intact cells and mitochondrial preparations - open discussion.<br />
::::::* B3. Towards a data base on coupling control in intact cells.<br />
<br />
****: '''2017-07-25'''<br />
:::: '''Session C''' (morning) '''Introduction: from experimental design to data analysis'''<br />
::::::* C1. Respiratory pathway control in mitochondrial preparations.<br />
::::::* C2. Substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles.<br />
::::::* C3. Normalization of respiratory flux and flow.<br />
::::::* C4. Respiratory control ratios and control factors.<br />
<br />
:::: '''Session D''' (afternoon) '''Posters and selected abstracts'''<br />
::::::* D1. Selected abstracts: applications of SUIT protocols – open discussion.<br />
::::::* D2. SUIT protocols: design and limitations.<br />
::::::* D3. Towards a data base on mitochondrial respiratory control: diagnostic approaches and comparative mitochondrial physiology.<br />
<br />
****: '''2017-07-26'''<br />
:::: '''Session E''' (morning) '''Mitochondrial physiology: biomedical applications''' (possible titles, depending on keynote lecturers/selected abstracts)<br />
::::::* E1. The challenges of functional mitochondrial diagnosis.<br />
::::::* E2. Mitochondrial fitness in skeletal muscle ([[Garcia-Roves PM |Pablo Garcia-Roves]], University of Barcelona, ES).<br />
::::::* E3. Scope and limitations of functional mitochondrial diagnosis in blood cells.<br />
::::::* E4. Mitochondrial respiratory function and neuromuscular disease.<br />
::::::* E5. Mitochondrial function and dysfunction in cancer ([[Porter RK |Richard K Porter]], Trinity College Dublin, IE).<br />
::::::* E6. Ischemia-reperfusion injury.<br />
::::::* E7. The metabolic syndrome ([[Lee HK |Hong Kyu Lee]], Eulji University College of Medicine, Seoul, KR).<br />
::::::* E8. Mitochondrial function in aging and regeneration ([[Duerr PJ |Pidder Jansen-Duerr]], University of Innsbruck, AT). <br />
<br />
:::: '''Session F''' (afternoon) '''Comparative mitochondrial physiology''' (possible titles, depending on keynote lecturers/selected abstracts)<br />
::::::* F1. Mitochondrial physiology in cell models versus primary tissue.<br />
::::::* F2. Mitochondrial physiology studies in mouse models.<br />
::::::* F3. Mitochondrial vertrebrate physiology – extreme performers.<br />
::::::* F5. Mitochondrial physiology of and beyond established animal models.<br />
::::::* F6. Mitochondrial physiology in plants and microbes.<br />
::::::* F7. Mitochondrial physiology and evolution.<br />
<br />
:::: '''Session G''' (evening) '''MITOEAGLE Early Career Investigators forum''' <br />
::::::* [[MITOEAGLE Early Career Investigators]]<br />
<br />
<br />
<br />
=== Preliminary programme MITOEAGLE Workshop WG1-4 (Jul 27-30) ===<br />
:::: currently in progress<br />
<br />
<br />
<br />
== Abstracts ==<br />
<br />
:::: Abstract submission: until '''May 31, 2017.'''<br />
:::: The abstract title has to be included on the registration form.<br />
<br />
:::* '''Categories''': Early career investigators (ECI) and students are invited to submit abstracts in four alternative categories and apply with their submission for a [[MiPschool_Obergurgl_2017#Support|MITOEAGLE Scholarship]]:<br />
<br />
::::# SUIT protocols: basic concepts. - » [[MitoPedia: SUIT]] <br />
::::# SUIT protocols: various applications with intact cells and mt-preparations.<br />
::::# Discuss definitions and controversies for a selected term on respiratory states and respiratory control ratios. - » [[MitoPedia: Respiratory states]], [[MitoPedia: Respiratory control ratios]]<br />
::::# Present your abstract as a definition of a term in the context of mitochondrial physiology that is missing in MitoPedia. - » [[MitoPedia]]<br />
<br />
:::* '''Format'''<br />
:::::: [[Abstract format]]<br />
:::::: '''Example''': [[Doerrier 2017 Abstract MITOEAGLE Barcelona]]<br />
<br />
<br />
:::* '''Abstract submission and review for MITOEAGLE Scholarships'''<br />
::::1. '''Eligibility:''' Early career investigators and students from all COST countries and International Partner Countries can apply - [[MITOEAGLE network]]. <br />
::::2. You need to select a 'Mentor' who will act as the contact to review your abstract and recommend you as a recipient of the MITOEAGLE scholarship.<br />
::::3. Check the [[MiPschool_Obergurgl_2017_Mentors|MITOEAGLE mentors website]] for the Training School, and select a 'Mentor'. <br />
::::4. Click on the highlighted name of the mentor for further details.<br />
::::::* Each mentor has two slots available. You can see in the column ''Booked'' whether the mentor is already booked or not. <br />
::::::*If you see that your preference no. 1 mentor already has one 'student', you might consider going for another mentor.<br />
::::5. Prepare your abstract in the format given above.<br />
::::::* Add the name of your selected mentor as a final section of your abstract.<br />
::::::* Cover letter: Provide a brief explanation to your selected mentor in a form of a cover letter.<br />
::::6. Send an Email to your mentor (and in Cc to mitoeagle@i-med.ac.at). Mention MITOEAGLE mentor in the subject line of your Email. Attach your abstract as a MS Word file and the cover letter.<br />
::::7. Experimental abstracts will be reviewed and accepted entirely independent of the experimental platform used in the study, and a wide variety of experimental approaches is highly welcome.<br />
<br />
::::» '''Notificiation of MITOEAGLE scholarships: 2017-06-10.'''<br />
<br />
<br />
== Support ==<br />
<br />
=== MITOEAGLE scholarships for Early Career Investigators and students ===<br />
<br />
::::* '''Submitted abstracts provide the basis for allocation of MITOEAGLE scholarships.<br />
::::* '''Proposed deadline: 2017-May-31'''.<br />
::::* '''42 basic scholarships''' will cover the local costs (accommodation, meals and registration). <br />
::::* Students from Inclusiveness Target Countries are eligible for '''extended scholarships''' covering travel costs in addition to the basic scholarship.<br />
<br />
::::* Abstracts submitted with data suitable for the data bases [[MITOEAGLE_Working_Groups|Working Groups 2, 3 and 4]] or [[WG1 MITOEAGLE protocols, terminology, documentation |Working Group 1]] are selected as a basis of additional support for joining the MITOEAGLE Workshop WG1-4 (Jul 27-30).<br />
::::* MITOEAGLE Working Group participants are supported (as far as possible) to join the Training School to be better prepared for the collaboration in the WG meeting, particularly the group working on the terminology review on respiratory states.<br />
<br />
::::» '''Notificiation of MITOEAGLE scholarships: 2017-06-10.'''<br />
<br />
<br />
<br />
<br />
[[File:Questions.jpg|left|60px]] <br />
::::» For further questions, please contact the '''MITOEAGLE representatives of the training schools''': <br />
:::::: [[Schlattner U |Uwe Schlattner]]<br />
:::::: [[Engin AB |Ayse Basak Engin]]<br />
::::» Further details on mentorship: consult [[MITOEAGLE Early Career Investigators]]<br />
::::» [[MITOEAGLE_network#Members_in_the_MITOEAGLE_network |List of MITOEAGLE mentors]]<br />
<br />
== Registration ==<br />
<br />
<br />
'''MiP''school'' registration Early Career Investigators and students'''<br />
::::» [[Media:Registration form Training School MITOEAGLE student.pdf| ECI and students registration]]<br />
<br />
::::» [[Media:Registration form Training School MITOEAGLE.pdf|Regular registration ]]<br />
<br />
<br />
'''MITOEAGLE Workshop WG1-4'''<br />
::::» [[Media:Registration_form_MITOEAGLE_Workshop_WG1-4.pdf|Registration form]]<br />
:::: Please send the completed form to [mailto:society@mitophysiology.org society@mitophysiology.org]<br />
<br />
<br />
== Travel info ==<br />
:::: How to get there? Find out more: [[MiPschool_Obergurgl_2017_Travel info|Travel info]]<br />
<br />
<br />
<br />
==Funding ==<br />
<br />
::::* [[COST Action MITOEAGLE |COST Action CA15203 Mitochondrial fitness mapping - MITOEAGLE]]<br />
<br />
[[Image:Tirol Logo Standortagentur.jpg|thumb|The project MitoFit is funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.|left| 100px]]<br />
[[Image:Medizinische-Uni-Innsbruck-Logo.gif|100px| Medical University Innsbruck]]<br />
[[Image:MitoFit.jpg|80px|link=http://www.mitofit.org/index.php/K-Regio MitoFit|K-Regio MitoFit]]<br />
<br />
<br />
<br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
<br />
<br />
{{MITOEAGLE banner}}<br />
{{Labeling<br />
|additional=2017, ORO, MitoFit, MITOEAGLE, Next<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Oroboros_Laboratory:_visiting_scientists&diff=135373Oroboros Laboratory: visiting scientists2017-05-10T08:46:52Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{OROBOROS header page name}}<br />
:::: <big>'''Following to the concept of open innovation and [[Gentle Science]], the [[OROBOROS MitoFit Laboratory]] welcomes visiting researchers from all over the world.'''</big><br />
<br />
[[File:OROBOROS MitoFit-Lab.jpg|left|240px|link=OROBOROS MitoFit Laboratory |MitoFit Laboratory]]<br />
[[Image:Logo MitoFit_s.jpg|right|100px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]]<br />
[[Image:O2k-Network.png|right|100px|link=O2k-Network|O2k-Network]]<br />
__TOC__<br />
::::» [[OROROBOS Science Scholarship]]s<br />
<br />
== 2017 ==<br />
{| class="wikitable" border="1"<br />
|-<br />
! From<br />
! Until<br />
! Visiting scientist<br />
! Country<br />
! [[O2k-Network |O2k-Network lab]] <br />
! Scholarship<br />
! Topic<br />
<br />
|-<br />
| Jan-15<br />
| Jul-15<br />
| [[Velika B|Beata Velika]]<br />
| SK<br />
| <br />
| [http://www.aktion.saia.sk/de Postdoc Scholarship Action Austria – Slovakia]<br />
| Human blood cells as study model of mitochondrial respiration and H2O2 production <br />
<br />
|-<br />
| Mar-01<br />
| Apr-25<br />
| [[Sobotka O|Ondrej Sobotka]]<br />
| CZ<br />
| [[CZ Hradec Kralove Cervinkova Z]]<br />
| [https://www.oead.at/index.php?id=2300&L=1 Postdoc Scholarship Action Austria – Czech Republic]<br />
| Methodological optimalization of continuous reactive oxygen species (ROS) measurement using fluorescent dye Amplex Ultra Red and O2k-fluorometry system<br />
<br />
|-<br />
| Feb-20<br />
| May-19<br />
|[[Permer M|Manuel Permer]]<br />
|AT <br />
|<br />
|<br />
|Respirometric characterization of cryopreserved HEK293T cells<br />
<br />
<br />
|-<br />
| May<br />
| Aug<br />
|[[Wohlfarter Y|Yvonne Wohlfarter]] (Internship)<br />
|AT<br />
| <br />
|<br />
|<br />
<br />
|-<br />
| Jun-25<br />
| Aug-25<br />
|[[Ghanim M|Magda Ghanim]]<br />
|IE<br />
|[[IE Dublin Porter RK]]<br />
|[[TRACT]]<br />
|HAMLET derivatives as a pre-operative therapy in oesophageal cancer<br />
<br />
|-<br />
| Jun-25<br />
| Aug-11<br />
|[[Karavyraki M|Marilena Karavyraki]]<br />
|IE<br />
|[[IE Dublin Porter RK]]<br />
|[[TRACT]]<br />
|Mitochondrial function linked to metabolic differences in normal, dysplastic and cancerous oral cells<br />
<br />
|-<br />
| Jul<br />
| Aug<br />
|[[Matic I|Ines Matic]] (Internship)<br />
|AT<br />
| <br />
|<br />
|<br />
|}<br />
<br />
<br />
== 2016 ==<br />
{| class="wikitable" border="1"<br />
|-<br />
! From<br />
! Until<br />
! Visiting scientist<br />
! Country<br />
! [[O2k-Network |O2k-Network lab]] <br />
! Scholarship<br />
! Topic<br />
<br />
|-<br />
| Feb-01<br />
| Feb-28<br />
| [[Bhattarai N|Nisha Bhattarai]]<br />
| US<br />
| [[US TX Galveston Porter C]]<br />
| [[OROROBOS Science Scholarship]]<br />
| mt-membrane potential<br />
<br />
|-<br />
| Feb-01<br />
| Mar-30<br />
| [[Chang SC|Chang Shao-Chiang]] <br />
| TW<br />
| [[TW Taoyuan Wang JS]]<br />
|<br />
| [[O2k-Fluorometer]]<br />
<br />
|-<br />
| Feb-01<br />
| Feb-07<br />
| [[Chou Chen-Hsien|Chen-Hsien Chou ]] <br />
| TW<br />
| [[TW Taoyuan Wang JS]]<br />
|<br />
| [[O2k-Fluorometer]]<br />
<br />
|-<br />
| Feb-15<br />
| Mar-15<br />
| [[Garcia-Souza LF|Luiz Felipe Garcia-Souza]]<br />
| BR<br />
| [[BR Rio de Janeiro Oliveira MF]]<br />
| [[MitoFit]]<br />
| [[MitoFit-Bloodcells]]<br />
<br />
|-<br />
| Mar-01<br />
| Jun-10<br />
| [[Sobotka O|Ondrej Sobotka]]<br />
| CZ<br />
| [[CZ Hradec Kralove Cervinkova Z]]<br />
| [http://www.cuni.cz/UKEN-158.html Charles University mobility fund]<br />
| [[Amplex red |ROS production]]<br />
<br />
|-<br />
| Mar-01<br />
| Apr-01<br />
| [[Volska K| Volska Kristine]] <br />
| LV<br />
| [[LV Riga Makrecka-Kuka M]]<br />
|<br />
| [[O2k-TPP+ ISE-Module]]<br />
<br />
|-<br />
| Apr-03<br />
| Jun-10<br />
| [[Jha RK| Jha Rajan Kumar]]<br />
| IN<br />
| [[IN Hyderabad Thangaraj K]]<br />
| [[OROROBOS Science Scholarship]]<br />
| [[MiPNet21.01_IOC_Schroecken_AT |O2k-Workshop]], [[O2k-Core |HRR]]<br />
<br />
|-<br />
| Jun-15<br />
| Jun-24<br />
| [[Jang D|David Jang]] <br />
| US<br />
| [[US PA Philadelphia Jang DH]]<br />
| [http://www.sfrbm.org/sections/education/fellowships SFRBM Mini-Fellowship Grant]<br />
| [[Amplex red |ROS production]], [[O2k-Publications:_Blood_cells |PBMCs]]<br />
<br />
|-<br />
| Nov-21<br />
| Nov-28<br />
| [[Meszaros A|Andras Meszaros]] <br />
| HU<br />
| [[HU Szeged Boros M]]<br />
| COST Action MITOEAGLE CA15203 [http://www.mitoglobal.org/index.php/Short-Term_Scientific_Missions_MITOEAGLE STSM Grant Period 1]<br />
| Harmonizing protocols of high resolution respirometry and fluorometry<br />
<br />
|-<br />
| Nov-21<br />
| Nov-28<br />
| [[Komlodi T|Timea Komlodi]] <br />
| HU<br />
| [[HU Budapest Tretter L]]<br />
| COST Action MITOEAGLE CA15203 [http://www.mitoglobal.org/index.php/Short-Term_Scientific_Missions_MITOEAGLE STSM Grant Period 1]<br />
| Standardization of protocols in mitochondria respirometry and fluorometry <br />
<br />
|-<br />
| Dec-12<br />
| Dec-16<br />
| [[Dymkowska D|Dorota Dymkowska]] <br />
| PL<br />
| [[PL Warsaw Zablocki K]]<br />
| COST Action MITOEAGLE CA15203 [http://www.mitoglobal.org/index.php/Short-Term_Scientific_Missions_MITOEAGLE STSM Grant Period 1]<br />
| Training in the MitoFit Laboratory <br />
<br />
|}<br />
----<br />
<br /><br />
::: <big>'''March 2017'''</big><br />
<br />
[[File:Sobotka O.JPG|left|400px|thumb|'''Ondrej Sobotka''']]<br />
:::: [[Sobotka O|Ondrej Sobotka]] (Czech Republic). Visiting scientist within the scholarship of the [http://www.dzs.cz/de/aktion-osterreich-tschechische-republik/stipendien/ AKTION Österreich – Tschechische Republik]. Mar 01 - Apr 25.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[CZ Hradec Kralove Cervinkova Z]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
::: <big>'''January 2017'''</big><br />
<br />
[[File:VelikaB.JPG|left|400px|thumb|'''Beata Velika''']]<br />
:::: [[Velika B|Beata Velika]] (Slovakia): Visiting scientist within the framework of the [http://www.aktion.saia.sk/de Postdoc Scholarship Action Austria]. Jan 15 - Jul 15. <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /> <br /><br /><br />
<br />
::: <big>'''December 2016'''</big><br />
<br />
:::: [[Dymkowska D|Dorota Dymkowska D]] (Hungary): Dec 12 - 16.<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[PL Warsaw Zablocki K]] <br />
<br /><br /><br />
<br />
::: <big>'''November 2016'''</big><br />
<br />
[[File:KomlodiTimea.JPG|left|400px|thumb|'''Timea Komlòdi''']]<br />
:::: [[Komlodi T|Timea Komlodi]] (Hungary): Nov 21 - 28.<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[HU Budapest Tretter L]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:Meszaros_Andras.JPG|left|400px|thumb|'''András Mészáros''']]<br />
:::: [[Meszaros A|Andras Meszaros]] (Hungary): Nov 21 - 28.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[HU Budapest Tretter L]] <br />
<br />
<br />
<br /><br /> <br /><br /> <br /> <br /><br /> <br /><br /> <br /> <br /><br /> <br /><br /><br />
::: <big>'''June 2016'''</big><br />
<br />
[[File:JangD.JPG|left|400px|thumb|'''David Jang''']]<br />
:::: [[Jang D|David Jang]] (USA): Visiting scientist within the framework of the [http://www.sfrbm.org/sections/education/fellowships SFRBM Mini-Fellowship Grant]. Jun 15 - Jul 15.<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] US PA Philadelphia Jang DH <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
::: <big>'''April 2016'''</big><br />
<br />
[[File:JhaRK.JPG|left|400px|thumb|'''Rajan Kumar Jha''']]<br />
:::: [[Jha RK|Rajan Kumar Jha]] (India): Apr 03 - Jun 10.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[IN Hyderabad Thangaraj K]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
::: <big>'''March 2016'''</big><br />
[[File:Volska K.JPG|left|400px|thumb|'''Kristine Volska''']]<br />
<br />
:::: [[Volska K| Kristine Volska]] (Latvia): Mar 01 - Apr 01.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[LV Riga Makrecka-Kuka M]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
<br />
[[File:Sobotka O.JPG|left|400px|thumb|'''Ondrej Sobotka''']]<br />
<br />
:::: [[Sobotka O|Ondrej Sobotka]] (Czech Republic): Visiting scientist within the framework of the [http://www.cuni.cz/UKEN-158.html Mobility fund of the Charles University]. Mar 01 - Jun 10.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[CZ Hradec Kralove Cervinkova Z]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
::: <big>'''February 2016'''</big><br />
[[File:Bhattarai N.JPG|left|400px|thumb|'''Nisha Bhattarai preparing an experiment''']]<br />
<br />
<br />
:::: [[Bhattarai N|Nisha Bhattarai]] (USA): Feb 01 - Feb 28.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[US TX Galveston Porter C]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
[[File:Chiang SC.JPG|left|400px|thumb|'''Shao-Chiang Chang is highly experienced with the O2k''']]<br />
<br />
:::: [[Chang SC|Shao-Chiang Chang]] (Taiwan): Feb 01 - Mar 30.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[TW Taoyuan Wang JS]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:Chou CH.JPG|left|400px|thumb|'''Chen-Hsien Chou generating data on HRR and proton flux''']]<br />
<br />
:::: [[Chou Chen-Hsien|Chen-Hsien Chou]] (Taiwan): Feb 01 - Feb 07.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[TW Taoyuan Wang JS]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
[[File:Garcia-Souza LF.JPG|left|400px|thumb|'''Luiz Felipe Garcia-Souza''']]<br />
<br />
:::: [[Garcia-Souza LF|Luiz Felipe Garcia-Souza]] (Brazil): Feb 15 - Mar 15.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[BR Rio de Janeiro Oliveira MF]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
== 2015 ==<br />
<br />
[[File:Bezuidenhout N.jpg|left|400px|thumb|'''Nicole Bezuidenhout tenderly preparing the O2k for an experiment]]<br />
::: <big>'''October 2015'''</big><br />
:::: [[Bezuidenhout N|Nicole Bezuidenhout]] (South Africa): Oct 05 - Nov 30.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[ZA Cape Town Ojuka EO]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:Spinazzi Marco.JPG|left|400px|thumb|'''Marko Spinazzi (right) analyzing data with Gerhard Krumschnabel]]<br />
:::: [[Spinazzi M|Marco Spinazzi]] (Belgium): Oct 21-22.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[BE Leuven Spinazzi M]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:Molina Tyrrell.JPG|left|400px|thumb|'''Daniel Tyrrell, Anthony Molina and Erich Gnaiger (f.l.t.r.) in front of the OROBOROS main office]]<br />
:::: [[Molina AJA| Anthony Molina]] and [[Tyrrell D| Daniel Tyrrell]] (USA): Oct 04-06.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[US NC Winston-Salem Molina AJA]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
[[File:McManus MJ.jpg |left|400px|thumb|'''[[McManus MJ| Meagan McManus]] preparing tissue for an experiment''']]<br />
::: <big>'''September 2015'''</big><br />
[[File:Meagan McManus.png|60px]] [[McManus MJ| Meagan J McManus]] (USA): Sep 14 - Oct 11.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[US PA Philadelphia Wallace DC]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
::::* Maryke Schoonen and Michelle Mereis (South Africa): Sep 28, University of Potchefstroom <br />
<br />
<br />
[[File:SiewieraK.JPG |left|400px|thumb|'''[[Doerrier C| Carolina Doerrier]] (left) and Karolina Siewiera experimenting on blood cells''']]<br />
::: <big>'''July 2015'''</big><br />
:::: [[Siewiera K| Karolina Siewiera]] (Poland): Visiting scientist within the framework of the [http://ec.europa.eu/programmes/erasmus-plus/discover/guide/index_en.htm ERASMUS]-programme. Jul 1 - Sep 30.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[PL Lodz Watala C]] <br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
::: <big>'''March 2015'''</big><br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Calabria E |Elisa Calabria]] (Italy): Mar 09 to Aug. - O2k-Network Lab: [[IT Verona Capelli C]]<br />
<br />
<br />
::: <big>'''February 2015'''</big><br />
<br />
:::: [[File:SumbalovaZ.JPG|60px]] [[Sumbalova Z| Zuzana Sumbalova]] (Slovak Republic): High-resolution measurement of mitochondrial membrane potential and respiration, combination of potentiometric (TPP+) and fluorometric (TMRM, safranin) methods; in the framework of [http://www.aktion.saia.sk/de Aktion Österreich – Slowakei]. Feb 01 to Jun 30. - O2k-Network Lab: [[SK Bratislava Sumbalova Z]]<br />
<br />
<br />
== 2014 ==<br />
<br />
::: <big>'''September 2014'''</big><br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Sumbalova Z| Zuzana Sumbalova]] (Slovak Republic): Sep 08 to Oct 04, [http://ec.europa.eu/programmes/erasmus-plus/discover/guide/index_en.htm ERASMUS] scholarship - MiP2014, IOC95 and [[malate]] project. - O2k-Network Lab: [[SK Bratislava Sumbalova Z]]<br />
<br />
<br />
[[File:Lab Kumphune Sarawut 1.JPG |left|400px|thumb|'''Sarawut Kumphune researching on mitochondrial function''']]<br />
::: <big>'''August 2014'''</big><br />
:::: [[File:Sarawut Kumphune.JPG|60px]] [[Kumphune S |Assist. Prof. Dr. Sarawut Kumphune]] (Thailand): Visiting scientist within the framework of the [http://www.asea-uninet.org/ ASEA-UNINET Programme]. Aug 2014 (one month).<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[TH Phitsanulok Kumphune S]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:Lab_MakreckaM_DavidikovaS.JPG|left|400px|thumb|'''Marina Makrecka (right) and Sarka Davidikova exploring the world of the O2k''']]<br />
:::: [[File:MakreckaM.jpg|60px]] [[Makrecka-Kuka M| Marina Makrecka-Kuka]] (Latvia): Visiting scientist within the framework of [http://ec.europa.eu/research/fp7/index_en.cfm/ FP7]. Aug-Sep 2014 (two months).<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[LV Riga Makrecka-Kuka M]]<br />
<br />
<br />
[[File:Sarka Davidikova.JPG|60px]] [[Davidikova S |Sarka Davidikova]] (Czech Republic): Visiting scientist within the framework of the [http://ec.europa.eu/programmes/erasmus-plus/discover/guide/index_en.htm ERASMUS]-programme. Aug-Nov 2014 (three months).<br />
<br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
::: <big>'''July 2014'''</big><br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Malacrida S| Sandro Malacrida]] (EURAC, Bolzano, IT), [[Pichler I |Irene Pichler]] (EURAC, Bolzano, IT), Alessandra Zenon (EURAC, Bolzano, IT) visited from Bolzano for a test experiment with their cultured cells. - O2k-Network Lab: [[IT Bolzano Pichler I]]<br />
<br />
<br />
[[File:MitoLab 1.JPG|left|400px|thumb|'''[[Krumschnabel G| Gerhard Krumschnabel]], Erika Nydlova and Martina Vrbova in the OROBOROS MitoLab''']]<br />
::: <big>'''May 2014'''</big><br />
:::: [[Vrbova Martina | Martina Vrbova]] (Czech Republic): May 2014.<br />
<br />
:::: [[Nydlova Erika | Erika Nydlova]] (Czech Republic): May 2014.<br />
<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[CZ Pardubice Rousar T]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
[[File:MitoLab 03.JPG|400px|left|thumb|'''Lindsay Benage performing an experiment''']]<br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Benage Lindsay | Lindsay Benage]] (USA): May 2014. - O2k-Network Lab: [[US CO Fort Collins Chicco AJ]]<br />
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
<br />
<br />
== 2013 ==<br />
<br />
::: <big>'''July 2013'''</big><br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Krautschneider M| Martin Krautschneider]] (Austria): Jul 01 to Aug 23. - O2k-Network Lab: [[AT Innsbruck Burtscher M]]<br />
<br />
<br />
::: <big>'''June 2013'''</big><br />
:::: [[File:GomezA.jpg|60px]] [[Gomez Rodriguez A| Gomez Rodriguez Alexia]] (Spain, Complutense University of Madrid ): Jun 04 to Jul 31<br />
<br />
<br />
::: <big>'''April 2013'''</big><br />
:::: [[Image:O2k-Network.png|40px|link=O2k-Network|O2k-Network]] [[Khalifa ARM| Abdel Rahman Medhat Khalifa]] (Egypt): Apr 15 to Jun 10, O2k-Network Lab: [[ET Giza Ali SS]]<br />
<br />
:::: [[File:Islam.jpg|60px]] [[Kotb IA| Islam Abo El Yazeed Kotb]] (Egypt): Apr 15 to Jun 10, O2k-Network Lab: [[ET Giza Ali SS]]<br />
<br />
<br />
== 2012 ==<br />
<br />
::: <big>'''October 2012'''</big><br />
:::: [[File:Gergö.jpg|60px]] [[Horvath Gergoe| Gergoe Horvath]] (Hungary): Visiting scientist within the framework of the [http://ec.europa.eu/programmes/erasmus-plus/discover/guide/index_en.htm| ERASMUS]-programme. Oct 2012 to Jan 2013, O2k-Network Lab: [[HU Budapest Tretter L]]</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=25th_Krakow_Conference_on_Endothelium_2017_PL&diff=13535725th Krakow Conference on Endothelium 2017 PL2017-05-09T13:26:48Z<p>Bitschnau Barbara: Created page with "{{MitoGlobal header page name}} {{Publication |title= Krakow PL, 2017 Oct 20-21. 25<sup>th</sup> Krakow Conference on Endothelium. |info= https://www.jmrc.org.pl |authors=Jagi..."</p>
<hr />
<div>{{MitoGlobal header page name}}<br />
{{Publication<br />
|title= Krakow PL, 2017 Oct 20-21. 25<sup>th</sup> Krakow Conference on Endothelium.<br />
|info= https://www.jmrc.org.pl<br />
|authors=Jagiellonian Medical Research Centre<br />
|year=2017-10-20<br />
|journal=MitoGlobal<br />
|abstract= 25<sup>th</sup> Krakow Conference on Endothelium, Krakow, Poland.<br />
|mipnetlab=<br />
}}<br />
<br />
__TOC__<br />
<br />
<br/><br />
== Venue ==<br />
:::: International Cultural Centre, Krakow on the Market Square<br />
::::» [http://mck.krakow.pl More information on the venue]<br />
<br />
<br />
<br/><br />
==Registration==<br />
::::'''Abstract Submission & Registration: '''<br />
:::: Details will follow soon. <br />
<br/><br />
== Programme ==<br />
:::: Details will follow soon. <br />
<br />
<br/><br />
<br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
{{Labeling<br />
|instruments=<br />
|additional=2017, ORO,<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=ISOTT_2017_Halle/Saale_DE&diff=135347ISOTT 2017 Halle/Saale DE2017-05-09T11:32:04Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoGlobal header page name}}<br />
{{Publication<br />
|title=[[File:ISOTT2017.jpg|60px|right |International Society on Oxygen Transport to Tissue (ISOTT)]] '''Halle/Saale DE''', 2017 Aug 19-23. 45<sup>th</sup> Annual Meeting of the International Society on Oxygen Transport to Tissue (ISOTT). <br />
|info=[http://isott2017.org www.isott2017.org]<br />
|authors= International Society on Oxygen Transport to Tissue<br />
|year=2017-08-19<br />
|journal=MitoGlobal<br />
|abstract= 6<sup>th</sup> 45th Annual Meeting of the International Society on Oxygen Transport to Tissue (ISOTT), Halle/Saale, Germany.<br />
|mipnetlab=<br />
}}<br />
<br />
__TOC__<br />
<br />
== Venue ==<br />
:::: Martin-Luther-University, Halle/Saale<br />
::::» [https://isott2017.org/conference-venue Information on venue]<br />
<br />
<br/><br />
==Registration==<br />
::::'''Abstract Submission & Registration: now open'''<br />
<br />
:::: [http://isott2017.org/online-registration Information on registration]<br />
:::: [http://isott2017.org/abstract-preparation Information on abstract submission]: Deadline for abstract submission is June 1st, 2017.<br />
<br />
<br />
<br/><br />
== Programme ==<br />
::::» [http://isott2017.org/preliminary-program Preliminary program]<br />
<br />
<br/><br />
== ISOTT awards ==<br />
<br />
:::: ISOTT is offering different awards to honor young scientists working in the field of oxygen transport (1.-3.) and to support the attendance of the ISOTT meeting (4.).<br />
::::» [https://isott2017.org/isott-awards More information]<br />
<br />
<br />
== Travel and accommodation==<br />
::::» [https://isott2017.org/accommodation More information]<br />
<br/><br />
<br/><br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
{{Labeling<br />
|instruments=<br />
|additional=2017, ORO,<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=ISOTT_2017_Halle/Saale_DE&diff=135346ISOTT 2017 Halle/Saale DE2017-05-09T11:30:49Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoGlobal header page name}}<br />
{{Publication<br />
|title=[[File:ISOTT2017.jpg|60px|right |International Society on Oxygen Transport to Tissue (ISOTT)]] '''Halle/Saale DE''', 2017 Aug 19-23. 45<sup>th</sup> Annual Meeting of the International Society on Oxygen Transport to Tissue (ISOTT). <br />
|info=[http://isott2017.org www.isott2017.org]<br />
|authors= International Society on Oxygen Transport to Tissue<br />
|year=2017-19-08<br />
|journal=MitoGlobal<br />
|abstract= 6<sup>th</sup> 45th Annual Meeting of the International Society on Oxygen Transport to Tissue (ISOTT), Halle/Saale, Germany.<br />
|mipnetlab=<br />
}}<br />
<br />
__TOC__<br />
<br />
== Venue ==<br />
:::: Martin-Luther-University, Halle/Saale<br />
::::» [https://isott2017.org/conference-venue Information on venue]<br />
<br />
<br/><br />
==Registration==<br />
::::'''Abstract Submission & Registration: now open'''<br />
<br />
:::: [http://isott2017.org/online-registration Information on registration]<br />
:::: [http://isott2017.org/abstract-preparation Information on abstract submission]: Deadline for abstract submission is June 1st, 2017.<br />
<br />
<br />
<br/><br />
== Programme ==<br />
::::» [http://isott2017.org/preliminary-program Preliminary program]<br />
<br />
<br/><br />
== ISOTT awards ==<br />
<br />
:::: ISOTT is offering different awards to honor young scientists working in the field of oxygen transport (1.-3.) and to support the attendance of the ISOTT meeting (4.).<br />
::::» [https://isott2017.org/isott-awards More information]<br />
<br />
<br />
== Travel and accommodation==<br />
::::» [https://isott2017.org/accommodation More information]<br />
<br/><br />
<br/><br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
{{Labeling<br />
|instruments=<br />
|additional=2017, ORO,<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=ISOTT_2017_Halle/Saale_DE&diff=135345ISOTT 2017 Halle/Saale DE2017-05-09T11:29:18Z<p>Bitschnau Barbara: Created page with "{{MitoGlobal header page name}} {{Publication |title=International Society on Oxygen Transport to Tissue (ISOTT) '''Halle/Saale DE''', 2017..."</p>
<hr />
<div>{{MitoGlobal header page name}}<br />
{{Publication<br />
|title=[[File:ISOTT2017.jpg|60px|right |International Society on Oxygen Transport to Tissue (ISOTT)]] '''Halle/Saale DE''', 2017 Aug 19-23. <sup>th</sup> 45th Annual Meeting of the International Society on Oxygen Transport to Tissue (ISOTT). <br />
|info=[http://isott2017.org www.isott2017.org]<br />
|authors= International Society on Oxygen Transport to Tissue<br />
|year=2017-19-08<br />
|journal=MitoGlobal<br />
|abstract= 6<sup>th</sup> 45th Annual Meeting of the International Society on Oxygen Transport to Tissue (ISOTT), Halle/Saale, Germany.<br />
|mipnetlab=<br />
}}<br />
<br />
__TOC__<br />
<br />
== Venue ==<br />
:::: Martin-Luther-University, Halle/Saale<br />
::::» [https://isott2017.org/conference-venue Information on venue]<br />
<br />
<br/><br />
==Registration==<br />
::::'''Abstract Submission & Registration: now open'''<br />
<br />
:::: [http://isott2017.org/online-registration Information on registration]<br />
:::: [http://isott2017.org/abstract-preparation Information on abstract submission]: Deadline for abstract submission is June 1st, 2017.<br />
<br />
<br />
<br/><br />
== Programme ==<br />
::::» [http://isott2017.org/preliminary-program Preliminary program]<br />
<br />
<br/><br />
== ISOTT awards ==<br />
<br />
:::: ISOTT is offering different awards to honor young scientists working in the field of oxygen transport (1.-3.) and to support the attendance of the ISOTT meeting (4.).<br />
::::» [https://isott2017.org/isott-awards More information]<br />
<br />
<br />
== Travel and accommodation==<br />
::::» [https://isott2017.org/accommodation More information]<br />
<br/><br />
<br/><br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
{{Labeling<br />
|instruments=<br />
|additional=2017, ORO,<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=File:ISOTT2017.jpg&diff=135344File:ISOTT2017.jpg2017-05-09T11:13:44Z<p>Bitschnau Barbara: </p>
<hr />
<div></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Wohlfarter_Yvonne&diff=135331Wohlfarter Yvonne2017-05-09T09:14:31Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{Person<br />
|lastname=Wohlfarter<br />
|firstname=Yvonne<br />
|institution=::::::::::::::::::::[[File:WohlfarterY.JPG|right|150px|Yvonne Wohlfarter]] <br />
'''OROBOROS INSTRUMENTS'''<br />
:: Mitochondria and cell research<br />
<br />
Yvonne Wohlfarter completed an internship as a trainee at [[OROBOROS_Contact |OROBOROS]] from 2016-06-24 to 2016-09-23.<br />
<br />
|address=Schöpfstr. 18<br />
|area code=6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress= Yvonne.Wohlfarter@gmx.at<br />
}}<br />
{{Labelingperson}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Sobotka_Ondrej&diff=135322Sobotka Ondrej2017-05-09T08:32:35Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{EAGLE<br />
|COST= Member<br />
|COST WG1= WG1<br />
|COST WG3= WG3<br />
}}<br />
{{Person<br />
|lastname=Sobotka<br />
|firstname=Ondrej<br />
|title=MUDr.<br />
|institution=::::::::::::::::::::::::::[[File:SobotkaO.JPG|right|150px|Ondrej Sobotka]] <br />
<br />
'''Charles University in Prague'''<br />
:: Faculty of Medicine in Hradec Kralove<br />
:: Department of Physiology<br />
<br />
'''Visiting scientist''' <br />
:* Research on: Mitochondria, metabolism, cell cultures, liver toxicity<br />
<br />
Ondrej Sobotka was a visiting scientist at the Medical University of Innsbruck <br/> within within the framework of the [http://www.dzs.cz/en/aktion-czech-republic-austria/scholarships Postdoc Scholarship AKTION Czech Republic-Austria] from March to April 2017.<br />
<br />
|address=Simkova 870<br />
|area code=500 38<br />
|city=Hradec Kralove<br />
|country=Czech Republic<br />
|mailaddress=sobotkao@lfhk.cuni.cz<br />
}}<br />
{{Labelingperson}}<br />
<br />
<br />
<br />
== Participated at ==<br />
* [[MITOEAGLE Barcelona 2017]]<br />
* [[IOC116]]<br />
* [[IOC109]]<br />
* [[IOC100]]<br />
* [[IOC95]]<br />
<br />
<br />
<br />
[[Image:O2k-Network.png|left|40px|link=O2k-Network|O2k-Network]]<br />
Visiting scientist at the [[OROBOROS MitoFit lab: visiting scientists|OROBOROS MitoFit Laboratory]] within the framework of the [http://www.cuni.cz/UKEN-158.html Mobility fund of the Charles University] from March 01 to June 10 2016.</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=TRACT_Kick-off_meeting_Dublin_IE&diff=135315TRACT Kick-off meeting Dublin IE2017-05-09T07:00:24Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoGlobal header page name}}<br />
{{Publication<br />
|title=[[Image:TRACT logo.png|right|170px|TRACT]] '''Dublin IE''', 2017 Mar 27. TRACT Kick-off meeting. <br />
|info=[[TRACT]]<br />
|authors=TRACT<br />
|year=2017-03-27<br />
|journal=MitoGlobal<br />
}}<br />
<br />
<br />
__TOC__<br />
== Programmes ==<br />
::::* Kick-off meeting - ''download pdf'': [[Media:TRACT_Kick_Off_agenda_27_March_17.pdf| '''Agenda TRACT Kick-off meeting''']] <br />
::::* General Assembly meeting (after Kick-off meeting) - ''download pdf'': [[Media:TRACT_General_Assembly_agenda_27_March_17.pdf| '''Agenda TRACT General Assembly meeting''']]<br />
<br />
== General information ==<br />
<br />
:::: '''TRACT Kick-off meeting ''' and '''General Assembly meeting''' (after the TRACT Kick-off meeting) <br />
:::: Date: '''2017-03-27'''<br />
:::: Venue: '''Trinity Biomedical Sciences Institute, Rm.L5.16<br />
:::: 152-160 Pearse Street<br />
:::: [https://www.tcd.ie/ University of Dublin, Trinity College], Dublin 2, Ireland'''<br />
<br />
<br />
== Speakers at the Kick-off meeting==<br />
:::: Prof. Jose V Bagan, University of Valencia, ES<br />
:::: Nicoleta Sinvici, Trinity College Dublin, IE<br />
:::: Dr Richard Turkington, Queen's University Belfast, IE<br />
:::: Dr Erich Gnaiger, Medical University of Innsbruck and OROBOROS INSTRUMENTS, AT<br />
:::: Prof Daniela Zisterer, Trinity College Dublin TCD, IE<br />
<br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
<br />
{{Labeling<br />
|additional=MitoGlobal, 2017, ORO<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=MitoEAGLE_Barcelona_2017&diff=135307MitoEAGLE Barcelona 20172017-05-08T15:07:14Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MITOEAGLE}}<br />
{{Publication<br />
|title=[[Image:MITOEAGLE-logo.jpg|right|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]] '''Barcelona ES''', 2017 Mar 21-23. Mitochondrial fitness mapping: Conference, Working Groups and MC meeting - [[COST Action MITOEAGLE]].<br />
|info=http://www.cost.eu/COST_Actions/ca/CA15203<br />
|authors=COST Association<br />
|year=2017-03-21<br />
|journal=MitoGlobal<br />
}}<br />
[[File:Barcelona ParcGueell.JPG|right|350px|Barcelona]]<br />
<br/><br />
[[File:Catalana.jpg|250px|right]]<br />
__TOC__<br />
<br />
<br />
:::: MITOEAGLE Conference, Working Groups and Management Committee Meeting<br />
:::: Date: '''2017-03-21 to 2017-03-23'''<br />
:::: Venue: '''[https://www.google.es/maps/@41.3817007,2.1692153,3a,75y,143.71h,95.59t/data=!3m6!1e1!3m4!1sVIb0BvhHADFA3EzqOeXiKQ!2e0!7i13312!8i6656!6m1!1e1 Institut d'Estudis Catalans Barcelona] - Sala Prat de la Riba, Barcelona, ES'''<br />
:::: Local organizer: [[Garcia-Roves PM |Pablo Garcia-Roves]], Department of Physiological Sciences II, Faculty of Medicine - University of Barcelona. - Email: [mailto:pgarciaroves@ub.edu pgarciaroves@ub.edu]<br />
<br />
<br />
== Minutes MITOEAGLE Barcelona 2017 ==<br />
:::: [[File:PDF.jpg|100px|link=http://www.mitoeagle.org/images/9/94/Barcelona2017_Minutes_CA15203.pdf |Click to open pdf file]] - Minutes prepared by [[Sumbalova Z |Zuzana Sumbalova]] and [[Laner V |Verena Laner]].<br />
<br />
<br />
== Feedback ==<br />
****: Dear colleagues,<br />
<br />
:::: As the local organizer of our e-COST MITOEAGLE meeting in Barcelona I want to thank you all for your participation and contribution to the success of this meeting. I hope you have enjoyed the meeting as much as I did. However, there is always some aspects that could be improved and your feedback could be valuable for future organizations.<br />
:::: If you have any comments or suggestions, please send it to our email: mitoeagle@i-med.ac.at so our next meetings could be even better.<br />
:::: Most of the meeting information (presentations and future actions) are being uploaded in our website: http://www.bioblast.at/index.php/MITOEAGLE_Barcelona_2017<br />
<br />
:::: We want to acknowledge the Catalonian Society of Biology as the hosting institution of the MITOEAGLE meeting for their support.<br />
<br />
:::: Many thanks and I hope to see you again in a near future,<br />
<br />
:::: With the best wishes,<br />
<br />
:::: Pablo - [[Garcia-Roves PM |Pablo Garcia-Roves, University of Barcelona, ES]]<br />
----<br />
<br />
<br />
:::: Dear Pablo:<br />
<br />
:::: Many thanks for taking the lead in organizing our first MITOEAGLE conference and helping our expanding group to make not only this meeting, but our ambitious [[COST Action MITOEAGLE |COST Action]] a great success. We are looking forward to receiving further feedback from all participants.<br />
<br />
:::: See you at our next MITOEAGLE event in Obergurgl – please, spread the news (42 scholarships and more) particularly to your students and early career investigators,<br />
<br />
:::: Erich - [[Gnaiger E |Erich Gnaiger, Medical University Innsbruck, AT]]<br />
::::» www.mitoeagle.org <br />
<br />
::::» http://www.mitoglobal.org/index.php/MiPschool_Obergurgl_2017 <br />
----<br />
<br />
<br />
****: Feedback from our participants<br />
:::* ''.. I really enjoyed the meeting in Barcelona. I have very rarely been to a meeting where I found so many of the presentations and scientists were thinking along the same lines. There is so much science to do and it seems that there is a good group of people who are attempting to get a better understanding of bioenergetics. ..'' - [[Chakrabarti L |Lisa Chakrabarti, University of Nottingham, UK]]<br />
:::* ''Once again congratulation for excellently organised MITOEAGLE meeting and for giving us opportunity to present our work and results.'' - [[Mars T|Tomaz Mars, University of Ljubljana, SI]]<br />
:::* ''First of all congratulation for the successful Mitoeagle event in Barcelona and for the organization!..'' - [[Giovarelli M|Matteo Giovarelli, Università di Milano, IT]]<br />
:::* ''As my Post-Doc informed me the meeting in Barcelona was super. Please let me know if we can somehow contribute on the MITOEAGLE review on terminology.'' - [[Trougakos IP |Ioannis Trougakos, National & Kapodistrian University of Athens, GR]]<br />
:::* ''Thank you again for Barcelona meeting. Wonderful conference, in the beautiful place, in a very nice atmosphere and perfectly organized'' - [[Labieniec-Watala M|Labieniec-Watala Magdalena, University of Lodz , PL]]<br />
:::* ''It was an amazing meeting in an amazing historical place. Everything, scientific and non-scientific, was perfectly organized. Thank you for local organizers. Hoping to attent next meeting.'' - [[Aral C |Cenk Aral, Namık Kemal University, Turkey]]<br />
:::* ''Thank you and all participants for such a great event. Very nice place, well organised and most important: interesting presentations in mitoeagle spirit.'' - [[Sobotka O |Sobotka Ondrej, Charles University, CZ]]<br />
:::* ''Hi Pablo, I would like first to express my thanks for the great organization of the Barcelona meeting. We enjoyed the meeting and the constructive discussion and also enjoyed the beautiful Barcelona.'' - [[Ali SS| Ali Sameh, Director of the Center for Aging and Associated Diseases, Egypt]]<br />
:::* ''Hi Pablo, just wanted to say thank you again for organizing a fantastic meeting in Barcelona. Please let me know how else I can contribute to the success of WG 2 as we move forward.'' - [[Coen PM| Coen Paul, Florida Hospital/Sanford Burnham, USA]]<br />
<br />
== Programme ==<br />
****: <big><big>Programme - ''download pdf'': [http://www.mitoglobal.org/images/5/59/Programme_MITOEAGLE_Barcelona_2017.pdf '''MITOEAGLE 2017 Barcelona ES''']</big></big> (last update: 2017-03-21)<br />
<br />
::::* Draft Agenda - Management Committee Meeting: [http://wiki.oroboros.at/images/9/96/Action_CA15203_MC_Agenda.pdf Action_CA15203_MC_Agenda.pdf]<br />
::::» [[Talk:MITOEAGLE_Barcelona_2017#Management_Committee_meeting |Management Committee - discussion and voting]] (last update: 2017-03-19)<br />
<br />
****: '''Time schedule'''<br />
::::* '''2017-03-21: MITOEAGLE Conference''' 09:00 - 18:00<br />
::::* '''2017-03-22: Working Group Meeting''' » [[WG1]] «» [[WG2]] «» [[WG3]] «» [[WG4]] «: 09:00 - 18:00<br />
::::* '''2017-03-23: [[Management Committee]] Meeting''' 09:00 - 13:00<br />
<br />
<br />
[[File:Barcelona ParcGueell.JPG|left|100px|MITOEAGLE Barcelona 2017]]<br />
== A: MITOEAGLE Conference ==<br />
=== A1: Connecting mitochondrial physiology - global perspectives and case studies ===<br />
:::: Tuesday, March 21, 09:15-11:10<br />
{{#ask:[[was submitted to event::MITOEAGLE Barcelona 2017]] [[Event::A1]]<br />
|?Event=Session<br />
|?has title=Presentation<br />
|?Mammal and model=Mammals;models<br />
|?Tissue and cell=Tissues;cells<br />
|?Diseases=Pathological states<br />
|format=broadtable<br />
|limit=500<br />
|offset=0<br />
|sort=<br />
|order=ascending<br />
}}<br />
<br />
=== A2: Connecting data on cardiac and sceletal muscle mitochondria ===<br />
:::: Tuesday, March 21, 11:40-13:45<br />
{{#ask:[[was submitted to event::MITOEAGLE Barcelona 2017]] [[Event::A2]]<br />
|?Event=Session<br />
|?has title=Presentation<br />
|?Mammal and model=Mammals;models<br />
|?Tissue and cell=Tissues;cells<br />
|?Diseases=Pathological states<br />
|format=broadtable<br />
|limit=500<br />
|offset=0<br />
|sort=<br />
|order=ascending<br />
}}<br />
<br />
=== A3: Connecting data on adipocyte mitochondria ===<br />
:::: Tuesday, March 21, 15:00-16:30<br />
{{#ask:[[was submitted to event::MITOEAGLE Barcelona 2017]] [[Event::A3]]<br />
|?Event=Session<br />
|?has title=Presentation<br />
|?Mammal and model=Mammals;models<br />
|?Tissue and cell=Tissues;cells<br />
|?Diseases=Pathological states<br />
|format=broadtable<br />
|limit=500<br />
|offset=0<br />
|sort=<br />
|order=ascending<br />
}}<br />
<br />
=== A4: Connecting data on cultured cells and blood cells ===<br />
:::: Tuesday, March 21, 17:00-19:00<br />
{{#ask:[[was submitted to event::MITOEAGLE Barcelona 2017]] [[Event::A4]]<br />
|?Event=Session<br />
|?has title=Presentation<br />
|?Mammal and model=Mammals;models<br />
|?Tissue and cell=Tissues;cells<br />
|?Diseases=Pathological states<br />
|format=broadtable<br />
|limit=500<br />
|offset=0<br />
|sort=<br />
|order=ascending<br />
}}<br />
<br />
<br />
[[File:Barcelona ParcGueell.JPG|left|100px|MITOEAGLE Barcelona 2017]]<br />
== B: MITOEAGLE Working Group meetings ==<br />
<br />
=== B1: Joint WGs meeting: Tasks and current status ===<br />
:::: Wednesday, March 22, 09:00-11:00<br />
{{#ask:[[was submitted to event::MITOEAGLE Barcelona 2017]] [[Event::B1]]<br />
|?Event=Session<br />
|?has title=Presentation<br />
|?Mammal and model=Mammals;models<br />
|?Tissue and cell=Tissues;cells<br />
|?Diseases=Pathological states<br />
|format=broadtable<br />
|limit=500<br />
|offset=0<br />
|sort=<br />
|order=ascending<br />
}}<br />
<br />
=== B2: Specific WG presentations ===<br />
:::: Wednesday, March 22, 11:30-13:30<br />
{{#ask:[[was submitted to event::MITOEAGLE Barcelona 2017]] [[Event::B2]]<br />
|?Event=Session<br />
|?has title=Presentation<br />
|?Mammal and model=Mammals;models<br />
|?Tissue and cell=Tissues;cells<br />
|?Diseases=Pathological states<br />
|format=broadtable<br />
|limit=500<br />
|offset=0<br />
|sort=<br />
|order=ascending<br />
}}<br />
<br />
=== B3: Specific WG meetings ===<br />
:::: Wednesday, March 22, 14:30-17:00<br />
{{#ask:[[was submitted to event::MITOEAGLE Barcelona 2017]] [[Event::B3]]<br />
|?Event=Session<br />
|?has title=Presentation<br />
|?Mammal and model=Mammals;models<br />
|?Tissue and cell=Tissues;cells<br />
|?Diseases=Pathological states<br />
|format=broadtable<br />
|limit=500<br />
|offset=0<br />
|sort=<br />
|order=ascending<br />
}}<br />
<br />
<br />
[[File:Barcelona ParcGueell.JPG|left|100px|MITOEAGLE Barcelona 2017]]<br />
<br />
<br />
== C: MITOEAGLE Management Committee Meeting ==<br />
<br />
Draft Agenda - Management Committee Meeting<br />
<br/>'''» Last update: 2017-03-22'''<br />
<br/><br />
::::'''1. Welcome to participants'''<br />
::::'''2. Verification of the presence of two-thirds of the Participating COST Countries or, if applicable, a quorum'''<br />
::::'''3. Adoption of agenda'''<br />
::::'''4. Approval of minutes and matters arising of last meeting''', please see: [[MITOEAGLE_Verona_2016#Minutes]]<br />
::::'''5. Update from the Action Chair'''<br />
:::::a) Status of Action: Start 2016-09-12, end of Action: 2020-09-11, participating COST countries, participating NNC/ IPC institutions and Specific Organisations.<br />
:::::: COST Members (countries) having accepted the MoU:<br />
::::::* ITCs: Number 14, non-ITCs: Number 16, Total: 30<br />
::::::* % of all: ITCs 46.67%, non-ITCs 53.33%<br />
:::::: Number of Action MC members: <br />
::::::* ITCs: 23, non-ITCs: 33, Total:56<br />
::::::* GP1: 2016-11-01 to 2017-04-30<br />
::::::* GP2: 2017-05-01 to 2018-04-30<br />
::::::* 163 participants, IPC:65, NNC:8, remaining Participants:231, MC:54, MC-Sub: 37<br />
:::::b) Short Term Scientific Missions (STSM): Review of completed reports and new applications by Magdalena Labieniec-Watala<br />
::::::* [[Short-Term_Scientific_Missions_MITOEAGLE]]<br />
::::''' 6. Update from the Grant Holder: Action budget status'''<br />
::::''' 7. Update from the COST Association: Mr. Peeters'''<br />
::::''' 8. Monitoring of the Action'''<br />
:::: ''' 9. Implementation of COST policies on:'''<br />
::::: a) Promotion of gender balance and Early Career Investigators (ECI)<br />
::::: b) Inclusiveness and Excellence (see below list of Inclusiveness Target Countries) reported by Tomaz Mars<br />
:::: '''10. Follow-up of MoU objectives: Progress report of working groups reported by WG leaders on day 2: 22.3.2017'''<br />
:::: '''11. Scientific planning'''<br />
::::: a) Scientific strategy (MoU objectives, GP Goals, WG tasks and deliverables)<br />
:::::: Aim/primary Objective: Include Evolution, Age, Gender, Lifestyle and Environment (EAGLE) in studies of mitochondrial function. MITOEAGLE develops harmonisation protocols towards generating a data repository on mitochondrial respiratory function. A data management system will interrelate various study-results and set them into a multidimensional context, to better diagnose mitochondrial respiratory defects.<br />
::::: b) Action Budget Planning<br />
:::::: '''Work and Budget Plan Summary'''<br />
:::::: '''A. COST Networking Tools EUR'''<br />
::::::: (1) Meetings 55,825.00 €<br />
::::::: (2) Training Schools 37,180.01 €<br />
::::::: (3) Short Term Scientific Missions (STSM) 35,000.00 €<br />
::::::: (4) COST Action Dissemination 2,800.00 €<br />
::::::: (5) Other Expenses Related to Scientific Activities (OERSA) 500.00 €<br />
:::::: '''B. Total Science Expenditure (sum of (1) to (5)) 131,305.01 €''' <br />
:::::: '''C. Financial and Scientific Administration'''<br />
:::::: Coordination (FSAC) (max. of 15% of B) 19,695.75 €<br />
:::::: Total Grant (B+C) 151,000.76 €<br />
::::::'''Information/Voting'''<br />
:::::* 280.75 € are left from the previous Verona meeting.<br />
:::::* Name tags for the Barcelona meeting: 29.14 €<br />
:::::* Remaining budget: 2905.86 € (dissemination)<br />
:::::* 2,905.86 € might be transferred to support participants at the Barcelona meeting.<br />
:::::* The Work and Budget Plan for Grant Period 2 has been submitted. Can the MC Meeting in Barcelona take care of the vote?<br />
:::::* Obergurgl MITOEAGLE Training School (MiPschool) and WG Meeting – flat rate for accommodation will be the price for the double room: 66.- €.<br />
:::::* Discuss the proposal for Training Schools: distribute partial support to many trainees rather than full support to few participants. Therefore, accommodation and meals for trainees will be covered by MITOEAGLE, and participants have to organize matching funds for travel.<br />
:::::* Cambridge Training School must be in March because the Grant Period 2 is ending in April, and an event in April would be too close to the end for completing the budget. As an alternative to the Cambridge Training School, funding for trainees at the Obergurgl Training School might be increased to cover travel costs in addition to accomodation, meals and registration.<br />
::::: c) Long-term planning (including anticipated locations and dates of future activities)<br />
::::::* [[MiPschool_Obergurgl_2017|Obergurgl AT, 2017 Jul 23-30. 10th MiPschool 2017 MITOEAGLE Science Camp]]<br />
::::::* [[MiP2017 Hradec Kralove CZ| Hradec Kralove CZ, 15-17 Nov 2017. 12th Conference on Mitochondrial Physiology: MITOEAGLE perspectives.]]<br />
::::: d) Dissemination planning (Publications and outreach activities): Update by Marina Makrecka-Kuka<br />
:::: '''12. Requests to join the Action from'''<br />
::::: a) COST countries: many from Spain - about 100 participants joined on e-COST since the last meeting. Mostly COST countries<br />
::::: b) Institutions in Near Neighbouring Countries, International Partner Countries, and/or Specific Organisations: EU agencies, European RTD Organisation, International Organisations: One request from Ghana<br />
:::: '''13. Any Other Business (AOB) - e.g. Russia to e-COST but already discussed in Verona'''<br />
:::: '''14. Location and date of next meeting'''<br />
:::::* Hradec Kralove CZ, 15-17 Nov 2017. 12th Conference on Mitochondrial Physiology: MITOEAGLE perspectives.<br />
:::: '''15. Summary of MC decisions'''<br />
:::: '''16. Closing'''<br />
<br />
<br />
=== Information ===<br />
::::* Excellent feedback was received on the circular for STSM host institutions: [[Short-Term_Scientific_Missions_MITOEAGLE#MITOEAGLE_host_institutions]] - Discuss future strategy (Magdalena Łabieniec-Watała).<br />
::::* MITOEAGLE flyer (with Impact) postponed: Instead of merely presenting the project proposal, first output and future perspectives can now be presented in the flyer. New goal: completion until November 2017 MC Meeting.<br />
<br />
::::* 280.75 € are left from the previous Verona meeting.<br />
::::* Name tags for the Barcelona meeting: 29.14 €<br />
::::* Remaining budget: 2905.86 € (dissemination)<br />
<br />
<br />
<div class="toccolours mw-collapsible mw-collapsed" style="background:#E0E6F8"><br />
<span style="font-size:105%; color:##424242">'''Voting'''</span><br />
<div class="mw-collapsible-content"><br />
::::* 2,905.86 € might be transferred to support participants at the Barcelona meeting.<br />
::::* The Work and Budget Plan for Grant Period 2 has been submitted. Can the MC Meeting in Barcelona take care of the vote?<br />
::::* Obergurgl MITOEAGLE Training School (MiPschool) and WG Meeting – flat rate for accommodation will be the price for the double room: 66.- €.<br />
::::* Discuss the proposal for Training Schools: distribute partial support to many trainees rather than full support to few participants. Therefore, accommodation and meals for trainees will be covered by MITOEAGLE, and participants have to organize matching funds for travel.<br />
::::* Cambridge Training School must be in March because the Grant Period 2 is ending in April, and an event in April would be too close to the end for completing the budget. As an alternative to the Cambridge Training School, funding for trainees at the Obergurgl Training School might be increased to cover travel costs in addition to accomodation, meals and registration.<br />
</div><br />
</div><br />
<br />
<br />
<div class="toccolours mw-collapsible mw-collapsed" style="background:#E0E6F8"><br />
<span style="font-size:105%; color:##424242">'''Voting information'''</span><br />
<div class="mw-collapsible-content"><br />
::::* Simple majority vote at a MC Meeting - decisions taken at MC Meetings by majority vote can only take effect if at least two-thirds of the Participating COST Countries are present and have their vote recorded – each Participating COST Country has one vote.<br />
::::* Members from International Partner Countries (IPC) can participate in Management Committee meetings as Observers, with no voting rights.</div><br />
</div><br />
<br />
<br/><br />
=== Budget plan Grant Period 2 ===<br />
::::* We intended to circulate the draft of the budget plan for discussion at our MC Meeting in Barcelona. To our surprise, however, this resulted in an automatic e-VOTE through e-COST. The present MC Meeting should provide the opportunity to elaborate the budget plan jointly, and to take a final vote during our meeting. <br />
<br />
<br />
=== MITOEAGLE MC meeting in 2018 in Serbia ===<br />
:::: I would like to confirm that we will be pleased to organize MITOEAGLE MC meeting in 2018 in Serbia. So, if it is on the agenda, we would be grateful to you if you could propose on behalf of the MC board the most convenient period of the year (grant period) to organize it with the options for the potential dates of the meeting during the next year.<br />
<br />
:::: '''Nebojsa Lalic'''<br />
:::: Professor Nebojsa M. Lalic<br />
:::: Faculty of Medicine, University of Belgrade<br />
:::: Clinic for Endocrinology, Diabetes and Metabolic Diseases<br />
:::: Clinical Center of Serbia<br />
:::: Dr Subotica 13, Belgrade 11000, Serbia<br />
<br />
<br />
[[File:Barcelona ParcGueell.JPG|left|100px|MITOEAGLE Barcelona 2017]]<br />
== General ==<br />
<br />
=== Registration and accomodation ===<br />
****: '''Registration'''<br />
::::* Download registration form: »[[Media:REGISTRATION FORM - MITOEAGLE meeting BARCELONA March 2017.docx |REGISTRATION FORM docx]]<br />
::::* Please send the registration to the Local Organizer [[Garcia-Roves PM |Pablo Garcia-Roves]] - [mailto:pgarciaroves@ub.edu pgarciaroves@ub.edu]<br />
::::* put [mailto:mitoeagle@i-med.ac.at mitoeagle@i-med.ac.at] in CC to receive an official invitation<br />
<br />
****: '''Accomodation''' - circular from the local organizer<br />
::::* 3 stars _ Hotel Turin:<br />
:::::: Individual room, breakfast included: 89,69 euros including taxes. <br />
:::::: Double room, breakfast included: 136,17 euros including taxes.<br />
:::::: Additional 0.72 euros per night and person as a touristic tax. <br />
:::::: Mention that you are booking in relation to “l’Institut d’Estudis Catalans”<br />
<br />
::::* 4 stars _ Hotel Silken Ramblas: (Ask for Rony and mention that you are coming from “Societat Catalana de Biologia”)<br />
:::::: Double room (individual room), breakfast included: 120,19 euros including taxes. <br />
:::::: Double room, breakfast included: 130 euros including taxes.<br />
:::::: Additional 1.21 euros as a touristic tax. <br />
<br />
::::* 5 stars _ Hotel Bagués:<br />
:::::: Send email to recepcionbagues@derbyhotels.com. Mention that you are coming from “Societat Catalana de Biologia” and they will apply to you their current offers and breakfast to a price of 16 instead of 21 euros.<br />
<br />
<br />
=== Agenda ===<br />
****: Prepared by the [[MITOEAGLE 2016 Verona IT |Core Group, Verona Meeting]]<br />
::::* Working Groups: milestones achieved.<br />
::::* Working Groups: next milestones.<br />
::::* '''Implementation strategy''': plan towards inclusiveness (geographical coverage, early career investigator involvement and gender balance) that is revised and updated at every MC meeting and develop a strategy to attract researchers and stakeholders.<br />
<br />
<br />
[[File:MITOEAGLE Working groups.jpg|right|300px|MITOEAGLE Working Groups]]<br />
****: '''Goals and outputs'''<br />
:::: Defined in the [[Media:WBP-AGA-CA15203-1.pdf| Work and Budget Plan Grant Period 1]]<br />
<br />
::: '''Goals'''<br />
::::* Draft of a manuscript on concepts and terminology of mitochondrial physiology, <br />
::::* Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a Library of protocols: reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis, <br />
::::* Draft of a manuscript on procedures, protocols and general guidelines for mitochondrial respiratory studies in muscle tissue,<br />
::::* Kick-off meeting discussing mt fitness evaluated in fat tissue,<br />
::::* Future applications, consensus protocols and reporting schemes in a standardized format.<br />
<br />
::: '''Outputs'''<br />
::::* Summary of actions and work plan by the MC.<br />
::::* Position paper on MITOEAGLE for publication continued.<br />
::::* Publications on nomenclature and terminology in mitochondrial physiology continued. <br />
::::* Roadmap for WG2 and WG3.<br />
::::* Training for selected students in methodological workshop.<br />
<br />
=== Support and budget ===<br />
::::* Support for travel, accommodation and daily allowance will be available through e-COST. <br />
::::* '''We aim at increasing the number of supported participants'''. The presently approved budget plan includes '''42 supported participants'''. Further participants are cordially invited at their own expense.<br />
::::* The Core Group suggested at the Verona meeting, that funding should be distributed widely across countries. With 30 countries listed for COST Action CA15203, our budget is limited to support one MC member per country ('''30 participants''') and provide additional support particularly to '''Early Career Investigators''' and participants from '''Inclusiveness Target Countries'''.<br />
::::* We will also try to provide partial support to participants with '''specific presentations''' according to the 'goals and outputs' defined in our COST Action.<br />
::::* Further topics and titles of presentations: please send your suggestions to the local organizer Pablo Garcia-Roves. <br />
::::* Management Committee (MC) Members: In case that you cannot join us this time, please inform us and your substitute, such that we can plan ahead the budget and the size of the MC meeting.<br />
::::* Final decisions on further financial support will be made on the basis of registrations received from the priorities (above).<br />
<br />
[[File:Barcelona ParcGueell.JPG|left|100px|MITOEAGLE Barcelona 2017]]<br />
== LOS informs - Dinner options ==<br />
<br />
::::''Dear all,''<br />
::::''We are getting closer to our meeting in Barcelona. More than 100 participants have registered. The number of participants makes it difficult to organize dinner for all together. We do not know how many people will be interested in joining, we could be a rather big group with short notice for the restaurants. Therefore, what we can do, as local organizers, it is to offer information on a wide variety of restaurants (different price range and food) so you can start getting organized, if you do not have additional information. As you may know there are hundreds of restaurants in walking distance from our venue to enjoy any type of food and drinks.'''<br />
<br />
::::''Under these lines you have some suggestions. On day 1 and 2 of our meeting there will be several people around that will be able to help you finding a restaurant or giving you a good advice.''<br />
<br />
::::''Looking forwards to our meeting''<br />
<br />
::::''Pablo M. Garcia-Roves''<br />
::::''Local organizer Barcelona MITOEAGLE meeting''<br />
<br/><br />
::::'''''RESTAURANTS for lunch or dinner (most of them not expensive)'''''<br />
::::'''L’ANTIC FORN''', address: Pintor Fortuny, 28<br />
::::'''BIOCENTER '''(vegetarian), address: Pintor Fortuny, 25<br />
::::'''FLOR DE MAIG''', address: Pintor Fortuny, 29 (corner Dr. Dou)<br />
::::'''SAGARRA''', address: Xuclà, 8 (corner Pintor Fortuny)<br />
::::'''SILENUS''', address: Àngels, 8<br />
::::'''FRESCO''', address: Carme, 16<br />
::::'''L’HORTET''' (vegetarian), address: Pintor Fortuny, 32<br />
<br />
<br />
::::'''''RESTAURANTS only dinner'''''<br />
::::'''Bar Cañete'''. Address: Unió 17. Famous tapas restaurant (expensive). Reservation: +34 932 703 458. http://barcanete.com/en/<br />
::::'''Hotel Le Meridien'''. Address: Rambla 111. Reservation: +34 93 318 6200. http://www.centonzerestaurant.com <br />
::::'''Fonda España'''. Address: Sant Pau, 9-11. Reservation: +34 93 550 00 00. https://www.hotelespanya.com/en/restaurante-fonda-espana-1/ <br />
<br />
<br />
::::'''''Other suggestions:'''''<br />
::::'''El Nacional'''. (Nice environment different options) Address: Passeig de Gràcia, 24 Bis. http://www.elnacionalbcn.com/en/ <br />
::::'''Senyor Parellada'''. (Catalonian traditional restaurant) Address: Argenteria, 37. http://www.senyorparellada.com <br />
::::'''Can Majó'''. (Seafood, paella) Address: Almirall Aixada nº23 (La Barceloneta). http://www.canmajo.es/en/ <br />
::::'''La mar salada'''. (Seafood, paella) Address: Pg. Joan de Borbó, 58-59. http://www.lamarsalada.cat/restaurant-eng.html <br />
<br />
<br />
[[File:Barcelona ParcGueell.JPG|left|100px|MITOEAGLE Barcelona 2017]]<br />
== List of participants ==<br />
{| class="wikitable sortable"<br />
|-<br />
!<br />
! style="text-align:center" |Country<br />
! Participant<br />
! <br />
|-<br />
|<br />
| style="text-align:center" |EG<br />
| [[Abdel-Rahman EA|Engy Ali]]<br />
| NNC<br />
|-<br />
| [[File:CalabriaE.jpg|right|90px|Elisa Calabria]]<br />
| style="text-align:center" |IT<br />
|[[Calabria E|Calabria Elisa]]<br />
| MC-Vice Chair<br />
|-<br />
| [[File:Cervinková Bioblast.JPG|right|90px|Zuzana Cervinkova]]<br />
| style="text-align:center" |CZ<br />
| [[Cervinkova Z|Cervinkova Zuzana]]<br />
| MC<br />
|-<br />
| [[File:DoerrierC.JPG|right|90px|Carolina Doerrier]]<br />
| style="text-align:center" |AT<br />
| [[Doerrier Velasco CA|Doerrier Velasco Carolina]]<br />
| MC-Substitute<br />
|-<br />
|<br />
| style="text-align:center" |TR<br />
| [[Engin AB|Engin Ayse Basak]]<br />
| MC<br />
|-<br />
| [[File:Garcia-RovesP.jpg|right|90px|Pablo Garcia-Roves ]]<br />
| style="text-align:center" |ES<br />
| [[Garcia-Roves PM|Garcia-Roves Pablo M]]<br />
| LOS<br />
|-<br />
| [[File:Gnaiger Erich.jpg|90px|right|Erich Gnaiger]]<br />
| style="text-align:center" |AT<br />
| [[Gnaiger E|Gnaiger Erich]]<br />
| Chair<br />
|-<br />
| <br />
| style="text-align:center" |EE<br />
| [[Kaambre T|Kaambre Tuuli]]<br />
| MC<br />
|-<br />
| [[File:Magda.JPG|right|90px|Magdalena Labieniec-Watala]]<br />
| style="text-align:center" |PL<br />
| [[Labieniec-Watala M|Labieniec-Watala Magdalena]]<br />
| MC<br />
|-<br />
| [[File:MakreckaM.jpg|right|90px|Marina Makrecka ]]<br />
| style="text-align:center" |LV<br />
| [[Makrecka-Kuka M|Makrecka-Kuka Marina]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center"|PL<br />
| [[Michalak Slawomir]]<br />
| MC<br />
|-<br />
| [[File:IMG 7832.JPG|100px|right|Kathrin Renner-Sattler]]<br />
| style="text-align:center" |DE<br />
| [[Renner-Sattler K|Renner-Sattler Kathrin]]<br />
| MC<br />
|-<br />
| [[File:ScatenaR.jpg|right|90px|Roberto Scatena]]<br />
| style="text-align:center" |IT<br />
| [[Scatena R|Scatena Roberto]]<br />
| MC<br />
|-<br />
|[[File:KomlodiT.JPG|right|100px|Komlodi Timea]]<br />
| style="text-align:center" |AT<br />
| [[Komlodi T|Komlodi Timea]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |EE<br />
| [[Vendelin M|Vendelin Marko]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |TR<br />
| [[Aral C|Aral Cenk]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Boada J|Boada Jordi]]<br />
| MC-Substitute<br />
|-<br />
|[[File:GorrT.jpg|right|100px|Thomas Gorr]]<br />
| style="text-align:center" |CH<br />
| [[Gorr TA|Gorr Thomas]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |RS<br />
| [[Jankovic A|Jankovic Aleksandra]]<br />
| MC-Substitute<br />
|-<br />
|<br />
| style="text-align:center" |DE<br />
| [[Klaus S|Klaus Susanne]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |SI<br />
| [[Kopitar-Jerala N|Kopitar-Jerala Natasa]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |NO<br />
| [[Aasum E|Aasum Ellen]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |LV<br />
| [[Liepins E|Liepins Edgars]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |PT<br />
| [[Palmeira C|Palmeira Carlos]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |DK<br />
| [[Nehlin J|Nehlin Jan]]<br />
| MC-Substitute<br />
|-<br />
|<br />
| style="text-align:center" |IT<br />
| [[Pieroni L|Pieroni Luisa]]<br />
| MC-Substitute<br />
|-<br />
|<br />
| style="text-align:center" |DK<br />
| [[Rasmussen LJ|Rasmussen Lene Juel]]<br />
| MC<br />
|-<br />
|[[File:RattanS.jpg|right|100px|Suresh Rattan]]<br />
| style="text-align:center" |DK<br />
| [[Rattan S|Rattan Suresh]]<br />
| MC<br />
|-<br />
|[[File:RustanAC.jpg|right|100px|Rustan Arild]]<br />
| style="text-align:center" |NO<br />
| [[Rustan AC|Rustan Arild Christian]]<br />
| MC-Substitute<br />
|-<br />
|[[File:SchlattnerU.jpg|right|100px|Uwe Schlattner]]<br />
| style="text-align:center" |FR<br />
| [[Schlattner U|Schlattner Uwe]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |EL<br />
| [[Tavernarakis N|Tavernarakis Nektarios]]<br />
| MC<br />
|-<br />
|[[File:TeppK.jpg|100px|right|Kersti Tepp]]<br />
| style="text-align:center" |EE<br />
| [[Tepp K|Tepp Kersti]]<br />
| MC-Substitute<br />
|-<br />
|[[File:20091010 Dominique VOTION Reduite.jpg|100px|right|Marie-Dominique Votion]]<br />
| style="text-align:center" |BE<br />
| [[Votion DM|Votion Dominique-Marie]]<br />
| MC<br />
|-<br />
|[[File:Eleonor A Frostner.jpg|right|100px|Eleonor Asander Frostner]]<br />
| style="text-align:center" |SE<br />
| [[Aasander Frostner E|Aasander Frostner Eleonor]]<br />
| WG<br />
|-<br />
|[[File:Casado PinnaM.jpg|right|100px|Marta Casado Pinna]]<br />
| style="text-align:center" |ES<br />
| [[Casado Pinna M|Casado Pinna Marta]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Fernandez-Moreno M|Fernandez-Moreno Mercedes]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |DE<br />
| [[Fromme T|Fromme Tobias]]<br />
| WG<br />
|-<br />
|[[File:MonsalveM.jpg|right|100px|Maria Monsalve]]<br />
| style="text-align:center" |ES<br />
| [[Monsalve M|Monsalve Maria]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |CZ<br />
| [[Stankova P|Stankova Pavla]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |FI<br />
| [[Lehti M|Lehti Maarit]]<br />
| MC<br />
|-<br />
|[[File:MarsT.jpg|right|100px|Tomaz Mars]]<br />
| style="text-align:center" |SI<br />
| [[Mars T|Mars Tomaz]]<br />
| MC<br />
|-<br />
|[[File:MoisoiN.jpg|right|100px|Nicoleta Moisoi]]<br />
| style="text-align:center" |UK<br />
| [[Moisoi N|Moisoi Nicoleta]]<br />
| MC<br />
|-<br />
|[[File:Murray Andrew J.jpg|right|100px|Murray Andrew James]]<br />
| style="text-align:center" |UK<br />
| [[Murray AJ|Murray Andrew James]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |SK<br />
| [[Velika B|Velika Beata]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |UK<br />
| [[Chakrabarti L|Chakrabarti Lisa]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |SE<br />
| [[Cannon B|Cannon Barbara]]<br />
| MC<br />
|-<br />
|[[File:Koopman WJH.JPG|100px|right|Werner Koopman]]<br />
| style="text-align:center" |NL<br />
| [[Koopman WJ|Koopman Werner J]]<br />
| MC<br />
|-<br />
|[[File:ArandarcikaiteO.jpg|100px|right| Odeta Arandarcikaite]]<br />
| style="text-align:center" |LT<br />
| [[Arandarcikaite O|Arandarcikaite Odeta]]<br />
| MC-Subsitute<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Herrero L|Herrero Laura]]<br />
| WG<br />
|-<br />
|[[File:Zorzano_A.jpg|right|100px|Zorzano Antonio]]<br />
| style="text-align:center" |ES<br />
| [[Zorzano A|Zorzano Antonio]]<br />
| MC-Substitute<br />
|-<br />
|[[File:Islam.jpg|100px|right|Kotb Islam]]<br />
| style="text-align:center" |SA<br />
| [[Kotb IA|Islam Aied]]<br />
| WG<br />
|-<br />
|[[File:PetitP.jpg|100px|right|Patrice Petit]]<br />
| style="text-align:center" |FR<br />
| [[Petit PX|Petit Patrice X]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |SK<br />
| [[Nemec M|Nemec Michal]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |IE<br />
| [[Porter RK|Porter Richard K]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |DK<br />
| [[Desler C|Desler Claus]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Villena JA|Villena Josep A]]<br />
| WG<br />
|-<br />
|[[File:Ali_S_photo.jpg|right|100px|Sameh Ali]]<br />
| style="text-align:center" |EG<br />
| [[Ali SS|Ali Sameh S]]<br />
| NNC<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Guitart M|Guitart Mariona]]<br />
| WG<br />
|-<br />
|[[File:ChabiB.JPG|right|100px|Beatrice Chabi]]<br />
| style="text-align:center" |FR<br />
| [[Chabi B|Chabi Beatrice]]<br />
| MC<br />
|-<br />
|[[File:NedergaardJ.jpg|right|100px|Jan Nedergaard]]<br />
| style="text-align:center" |SE<br />
| [[Nedergaard J|Nedergaard Jan]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Gama Perez P|Gama Perez Pau]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Quiles JL|Quiles José L]]<br />
| <br />
|-<br />
|[[File:SobotkaO.JPG|right|100px|Ondrej Sobotka]]<br />
| style="text-align:center" |CZ<br />
| [[Sobotka O|Sobotka Ondrej]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Claret M|Claret Marc]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Peral B|Peral Belén]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Juarez D|Juárez Diana L]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |IT<br />
| [[Giovarelli M|Giovarelli Matteo]]<br />
| WG<br />
|-<br />
|[[File:VG.jpg|right|100px|Laner Verena]]<br />
| style="text-align:center" |AT<br />
| [[Laner V|Laner Verena]]<br />
| MC-Substitute<br />
|-<br />
|[[File:SiewieraK.JPG|right|100px|Karolina Siewiera]]<br />
| style="text-align:center" |PL<br />
| [[Siewiera K|Siewiera Karolina]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Blanco FJ|Blanco Franciscno J]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |QA<br />
| [[Guarch ME|Guarch Meritxell Espino]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Gil J|Gil Joan]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |NO<br />
| [[Lund T|Lund Trine]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |BE<br />
| [[Sonveaux P|Sonveaux Pierre]]<br />
| MC<br />
|-<br />
|<br />
| style="text-align:center" |NL<br />
| [[Wuest RC|Wuest Rob C]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
| [[Lopez Garcia LC|López García Luis Carlos]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |NL<br />
| [[Keijer J|Keijer Jaap]]<br />
| MC<br />
|-<br />
|[[File:PaulCoen.jpg|right|100px|Paul Coen]]<br />
| style="text-align:center" |US<br />
| [[Coen PM|Coen Paul M]]<br />
| Invited Speaker<br />
|-<br />
|<br />
| style="text-align:center" |EL<br />
| [[Gumeni S|Gumeni Sentiljana]]<br />
| MC-Substitute<br />
|-<br />
|[[File:Ost M.jpg|right|100px|Mario Ost]]<br />
| style="text-align:center" |DE<br />
| [[Ost M|Ost Mario]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |UK<br />
| [[Iglesias-Gonzalez J|Iglesias-Gonzalez Javier]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |AT<br />
| [[Bufe A|Bufe A]]<br />
| WG<br />
|-<br />
|[[File:ShabalinaIG.jpg|100px|right|Irina G Shabalina]]<br />
| style="text-align:center" |SE<br />
| [[Shabalina IG|Shabalina Irina G]]<br />
| WG<br />
|-<br />
|[[File:Zorzano sebastian.jpg|right|100px]]<br />
| style="text-align:center" |ES<br />
| [[Sebastian D|Sebastian David]]<br />
| WG<br />
|-<br />
|<br />
| style="text-align:center" |UK<br />
|[[Garten A|Garten Antje]]<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
|[[De la Torre Lara J|De la Torre Lara Joan]]<br />
|-<br />
|[[File:Olga foto.jpg|right|100px]]<br />
| style="text-align:center" |ES<br />
|[[Tura Ceide O|Tura Ceide Olga]]<br />
|-<br />
|<br />
| style="text-align:center" |DE<br />
|[[Hartmann C|Hartmann Clair]]<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
|[[Quintana A|Quintana Albert]]<br />
|-<br />
|<br />
| style="text-align:center" |DE<br />
|[[Koenig M|Koenig Magdalena]]<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
|[[Soler Vazquez DC|Soler Vazquez Maria del Carmen]]<br />
|-<br />
|<br />
| style="text-align:center" |FR<br />
|[[Dubouchaud H|Dubouchaud Hervé]]<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
|[[Batlle M|Batlle Montserrat]]<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
|[[Smolders V|Smolders Valérie ]]<br />
|-<br />
|<br />
| style="text-align:center" |DE<br />
|[[Thinnes A|Thinnes Anna]]<br />
|-<br />
|<br />
| style="text-align:center" |NO<br />
|[[Larsen TS|Larsen Terje S]]<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
|[[Sabate A|Sabate Alba]]<br />
|-<br />
|[[File:SumbalovaZ.JPG|right|100px|Zuzana Sumbalova]]<br />
| style="text-align:center" |AT<br />
|[[Sumbalova Z|Sumbalova Zuzana]]<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
|[[Moren C|Moren Constanza]]<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
|[[Garrabou G|Garrabou Gloria]]<br />
|-<br />
|<br />
| style="text-align:center" |ES<br />
|[[Gonzalez Casacuberta I|Gonzalez Casacuberta Ingrid]]<br />
|}<br />
<br />
<br />
[[File:Barcelona ParcGueell.JPG|left|100px|MITOEAGLE Barcelona 2017]]<br />
== All abstracts - alphabetical order==<br />
<br />
{{#ask:[[was submitted to event::MITOEAGLE Barcelona 2017]] <br />
|?Event=Session<br />
|?has title=Presentation<br />
|format=broadtable<br />
|limit=500<br />
|offset=0<br />
|sort=<br />
|order=ascending<br />
}}<br />
<br />
<br />
[[File:Barcelona ParcGueell.JPG|left|100px|MITOEAGLE Barcelona 2017]]<br />
== Satellite O2k-Workshop ==<br />
::::* Date: Monday, 2017-03-20<br />
::::* Venue: Institute for Research in Biomedicine (IRB Barcelona), Facultat de Biologia, Universitat de Barcelona<br />
::::* Local organier: [[Zorzano A |Antonio Zorzano]] - [[ES Barcelona Zorzano A |O2k-Network laboratory: ES Barcelona Zorzano A]]<br />
::::* O2k-Fluorometer demo experiment: high-resolution respirometry and H<sub>2</sub>O<sub>2</sub> production (Amplex red)<br />
::::* Separate registration<br />
::::» More details: [[MiPNet22.04 IOC120 Barcelona ES |'''IOC120''']]<br />
<br />
<br />
== Previous MITOEAGLE meeting ==<br />
::::* [[MITOEAGLE Verona 2016]]<br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
{{Labeling<br />
|additional=ORO, 2017, MITOEAGLE<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=JSBBA_2017_Kyoto_JP&diff=135306JSBBA 2017 Kyoto JP2017-05-08T15:05:52Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoGlobal header page name}}<br />
{{Publication<br />
|title=[[File:JSBBA.jpg|60px|right|Japan Society for Bioscience, Biotechnology, and Agrochemistry]] '''Kyoto JP''', 2017 Mar 18-20. 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA)<br />
|info=[http://www.jsbba.or.jp/2017/english/ JSBBA]<br />
|authors=Japan Society for Bioscience, Biotechnology, and Agrochemistry<br />
|year=2017-03-18<br />
|journal=MitoGlobal<br />
|abstract= 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA), Kyoto, Japan<br />
|mipnetlab=<br />
}}<br />
<br />
__TOC__<br />
<br />
== Venue ==<br />
::::[http://www.kyoto-wu.ac.jp/english/index.html Kyoto Women's University]<br />
::::Hiyoshi-Cho 35, Imakumano Nokita, Higashiyama-ku, Kyoto, Japan 605-8501 ([https://www.google.co.jp/maps/place/Kyoto+Women's+University/@34.9895358,135.7770759,17z/data=!4m13!1m7!3m6!1s0x60010f32c5bb1c35:0x2f646b6b2b9ddcff!2s35+Imagumano+Kitahiyoshich%C5%8D,+Higashiyama-ku,+Ky%C5%8Dto-shi,+Ky%C5%8Dto-fu+605-0926!3b1!8m2!3d34.9895358!4d135.7792646!3m4!1s0x0:0x6c8db6dcc22cbcd4!8m2!3d34.9886048!4d135.7798422 view on map])<br />
<br />
==Registration==<br />
::::Registration-Form, more information about registration fees, and other details can be found [http://www.jsbba.or.jp/2017/english/ here]<br />
<br />
<br />
== Programme ==<br />
::::[http://www.jsbba.or.jp/2017/program_e.html Programme for March 18-20]<br />
<br />
<br />
== Organizer ==<br />
::::Chairperson: Prof. Shuji Adachi, Graduate School of Agriculture, Kyoto University<br />
<br />
== OROBOROS Representative==<br />
::::OROBOROS will be represented by the official distributor [[JP Tokyo Sanyo|Sanyo Trading Co., Ltd.]]<br />
<br />
<br />
<br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
<br />
{{Labeling<br />
|instruments=<br />
|additional=2017, ORO<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=MiPschool_Obergurgl_2017&diff=135299MiPschool Obergurgl 20172017-05-08T13:04:25Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MiP header page name}}<br />
{{Publication<br />
|title=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]]<br />
[[Image:MiPsocietyLOGO.JPG|right|120px|link=http://www.mitophysiology.org|MiPsociety]]<br />
'''Obergurgl AT''', 2017 Jul 23-30. '''10<sup>th</sup> MiP''school'' 2017 MITOEAGLE and MITOEAGLE Workshop WG1-4.<br />
|info=<br />
|authors=Mitochondrial Physiology Society<br />
|year=2017-07-23<br />
|journal=MitoGlobal<br />
|abstract='''2017 Jul 23-30, Obergurgl, AT.''' <br />
|mipnetlab=<br />
}}<br />
[[File:MUI Logo rz rgb.jpg|150px|right|link=https://www.i-med.ac.at |Medical University Innsbruck]]<br />
[[File:Logo_age_reg.jpg|150px|right|link=https://www.uibk.ac.at/forschung/doktoratskollegs/index.html.de| DK Ageing and Regeneration]]<br />
[[File:Logo_Univ_Innsbruck.jpg|150px|right|link=https://www.uibk.ac.at |University of Innsbruck]]<br />
<br />
<br />
__TOC__<br />
== Date, venue, organization ==<br />
::::* 2017 July 23-30<br />
:::::* 2017 July 23-27 Training School jointly organized by the [[Mitochondrial Physiology Society]] and [[MC_MITOEAGLE_e-VOTE_02#Topic_.282.29_Training_School_in_Obergurgl.2C_Tyrol.2C_AT_in_July_2017 |COST Action MITOEAGLE]]<br />
:::::* 2017 July 27-30 MITOEAGLE Workshop: [[MITOEAGLE_Working_Groups|WG1-4]]<br />
::::* [http://www.uz-obergurgl.at University Centre Obergurgl], Tyrol, Austria<br />
<br />
<br />
== Programme ==<br />
<br />
=== Programme structure ===<br />
::::* '''2017-07-23, Day 1:''' Arrival<br />
::::* '''2017-07-24 to 26, Day 2-4: Training school MiP''society'' and MITOEAGLE''' <br />
::::** Day 2-3: Intoductory lectures on the mitochondrial electron transfer system (ETS), coupling of electron transfer to proton translocation and phosphorylation (OXPHOS), mitochondrial pathways, respiratory protocols for diagnosis of mitochondrial function, mitochondrial respiratory control in health and disease.<br />
::::** Day 4: Scientific lectures and student presentations<br />
::::* '''2017-07-27, Day 5:''' Departure Training school, arrival MITOEAGLE workshop, relaxing day for overall-participants<br />
::::* '''2017-07-28 to 29, Day 6-7: MITOEAGLE Workshop: WG1-4'''<br />
::::** '''WG1: Harmonization of nomenclature on mitochondrial respiratory states and control parameters:''' Status of the ''consensus paper'' on a consistent terminology on mitochondrial physiology and bioenergetics; recommendations for the use of a common terminology in mitochondrial physiological research as a milestone towards unification of concepts and nomenclature.<br />
::::** '''WG1: Respirometric reference protocols:''' (i) Standard format for presenting substrate-uncoupler-inhibitor titration (SUIT) protocols for a ‘library of protocols’ applied in mitochondrial respiratory physiology; (ii) criteria for selecting and recommending reference protocols; (iii) documentation of the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating SUIT protocols (compare: Maelstrom Research program).<br />
::::** '''WG2-4: Data repositories'''<br />
::::* '''2017-07-30, Day 8:''' Departure MITOEAGLE<br />
<br />
=== Preliminary programme MiP''school'' (Jul 23-27) ===<br />
<br />
:::: This MiP''school'' will focus on basic concepts on mitochondrial respiratory states and applications of substrate-uncoupler-inhibitor titration (SUIT) protocols. <br />
<br />
****: '''2017-07-24'''<br />
:::: '''Session A''' (morning) '''Introduction: coupling control in oxidative phosphorylation'''<br />
::::::* A1. The electron transfer system – mitochondrial pathways from fuel substrates to oxygen.<br />
::::::* A2. Coupling of the phosphorylation system to electron transfer and respiratory coupling control.<br />
::::::* A3. From Einstein's diffusion equation to Mitchell’s chemiosmotic equation: rates and states in the bioenergetics of oxidative phosphorylation.<br />
::::::* A4. Measurement of coupling in intact cells and mitochondrial preparations: biochemical coupling efficiency and ATP/O2 ratios.<br />
<br />
:::: '''Session B''' (afternoon) '''Posters and selected abstracts'''<br />
::::::* B1. Poster session.<br />
::::::* B2: Selected abstracts: measurement of coupling control in intact cells and mitochondrial preparations - open discussion.<br />
::::::* B3. Towards a data base on coupling control in intact cells.<br />
<br />
****: '''2017-07-25'''<br />
:::: '''Session C''' (morning) '''Introduction: from experimental design to data analysis'''<br />
::::::* C1. Respiratory pathway control in mitochondrial preparations.<br />
::::::* C2. Substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles.<br />
::::::* C3. Normalization of respiratory flux and flow.<br />
::::::* C4. Respiratory control ratios and control factors.<br />
<br />
:::: '''Session D''' (afternoon) '''Posters and selected abstracts'''<br />
::::::* D1. Selected abstracts: applications of SUIT protocols – open discussion.<br />
::::::* D2. SUIT protocols: design and limitations.<br />
::::::* D3. Towards a data base on mitochondrial respiratory control: diagnostic approaches and comparative mitochondrial physiology.<br />
<br />
****: '''2017-07-26'''<br />
:::: '''Session E''' (morning) '''Mitochondrial physiology: biomedical applications''' (possible titles, depending on keynote lecturers/selected abstracts)<br />
::::::* E1. The challenges of functional mitochondrial diagnosis.<br />
::::::* E2. Mitochondrial fitness in skeletal muscle ([[Garcia-Roves PM |Pablo Garcia-Roves]], University of Barcelona, ES).<br />
::::::* E3. Scope and limitations of functional mitochondrial diagnosis in blood cells.<br />
::::::* E4. Mitochondrial respiratory function and neuromuscular disease.<br />
::::::* E5. Mitochondrial function and dysfunction in cancer ([[Porter RK |Richard K Porter]], Trinity College Dublin, IE).<br />
::::::* E6. Ischemia-reperfusion injury.<br />
::::::* E7. The metabolic syndrome ([[Lee HK |Hong Kyu Lee]], Eulji University College of Medicine, Seoul, KR).<br />
::::::* E8. Mitochondrial function in aging and regeneration ([[Duerr PJ |Pidder Jansen-Duerr]], University of Innsbruck, AT). <br />
<br />
:::: '''Session F''' (afternoon) '''Comparative mitochondrial physiology''' (possible titles, depending on keynote lecturers/selected abstracts)<br />
::::::* F1. Mitochondrial physiology in cell models versus primary tissue.<br />
::::::* F2. Mitochondrial physiology studies in mouse models.<br />
::::::* F3. Mitochondrial vertrebrate physiology – extreme performers.<br />
::::::* F5. Mitochondrial physiology of and beyond established animal models.<br />
::::::* F6. Mitochondrial physiology in plants and microbes.<br />
::::::* F7. Mitochondrial physiology and evolution.<br />
<br />
:::: '''Session G''' (evening) '''MITOEAGLE Early Career Investigators forum''' <br />
::::::* [[MITOEAGLE Early Career Investigators]]<br />
<br />
<br />
<br />
=== Preliminary programme MITOEAGLE Workshop WG1-4 (Jul 27-30) ===<br />
:::: currently in progress<br />
<br />
<br />
<br />
== Abstracts ==<br />
<br />
:::: Abstract submission: until '''May 31, 2017.'''<br />
:::: The abstract title has to be included on the registration form.<br />
<br />
:::* '''Categories''': Early career investigators (ECI) and students are invited to submit abstracts in four alternative categories and apply with their submission for a [[MiPschool_Obergurgl_2017#Support|MITOEAGLE Scholarship]]:<br />
<br />
::::# SUIT protocols: basic concepts. - » [[MitoPedia: SUIT]] <br />
::::# SUIT protocols: various applications with intact cells and mt-preparations.<br />
::::# Discuss definitions and controversies for a selected term on respiratory states and respiratory control ratios. - » [[MitoPedia: Respiratory states]], [[MitoPedia: Respiratory control ratios]]<br />
::::# Present your abstract as a definition of a term in the context of mitochondrial physiology that is missing in MitoPedia. - » [[MitoPedia]]<br />
<br />
:::* '''Format'''<br />
:::::: [[Abstract format]]<br />
:::::: '''Example''': [[Doerrier 2017 Abstract MITOEAGLE Barcelona]]<br />
<br />
<br />
:::* '''Abstract submission and review for MITOEAGLE Scholarships'''<br />
::::1. '''Eligibility:''' Early career investigators and students from all COST countries and International Partner Countries can apply - [[MITOEAGLE network]]. <br />
::::2. You need to select a 'Mentor' who will act as the contact to review your abstract and recommend you as a recipient of the MITOEAGLE scholarship.<br />
::::3. Check the [[MiPschool_Obergurgl_2017_Mentors|MITOEAGLE mentors website]] for the Training School, and select a 'Mentor'. <br />
::::4. Click on the highlighted name of the mentor for further details.<br />
::::::* Each mentor has two slots available. You can see in the column ''Booked'' whether the mentor is already booked or not. <br />
::::::*If you see that your preference no. 1 mentor already has one 'student', you might consider going for another mentor.<br />
::::5. Prepare your abstract in the format given above.<br />
::::::* Add the name of your selected mentor as a final section of your abstract.<br />
::::::* Cover letter: Provide a brief explanation to your selected mentor in a form of a cover letter.<br />
::::6. Send an Email to your mentor (and in Cc to mitoeagle@i-med.ac.at). Mention MITOEAGLE mentor in the subject line of your Email. Attach your abstract as a MS Word file and the cover letter.<br />
::::7. Experimental abstracts will be reviewed and accepted entirely independent of the experimental platform used in the study, and a wide variety of experimental approaches is highly welcome.<br />
<br />
::::» '''Notificiation of MITOEAGLE scholarships: 2017-06-10.'''<br />
<br />
<br />
== Support ==<br />
<br />
=== MITOEAGLE scholarships for Early Career Investigators and students ===<br />
<br />
::::* '''Submitted abstracts provide the basis for allocation of MITOEAGLE scholarships.<br />
::::* '''Proposed deadline: 2017-May-31'''.<br />
::::* '''42 basic scholarships''' will cover the local costs (accommodation, meals and registration). <br />
::::* Students from Inclusiveness Target Countries are eligible for '''extended scholarships''' covering travel costs in addition to the basic scholarship.<br />
<br />
::::* Abstracts submitted with data suitable for the data bases [[MITOEAGLE_Working_Groups|Working Groups 2, 3 and 4]] or [[WG1 MITOEAGLE protocols, terminology, documentation |Working Group 1]] are selected as a basis of additional support for joining the MITOEAGLE Workshop WG1-4 (Jul 27-30).<br />
::::* MITOEAGLE Working Group participants are supported (as far as possible) to join the Training School to be better prepared for the collaboration in the WG meeting, particularly the group working on the terminology review on respiratory states.<br />
<br />
::::» '''Notificiation of MITOEAGLE scholarships: 2017-06-10.'''<br />
<br />
<br />
<br />
<br />
[[File:Questions.jpg|left|60px]] <br />
::::» For further questions, please contact the '''MITOEAGLE representatives of the training schools''': <br />
:::::: [[Schlattner U |Uwe Schlattner]]<br />
:::::: [[Engin AB |Ayse Basak Engin]]<br />
::::» Further details on mentorship: consult [[MITOEAGLE Early Career Investigators]]<br />
::::» [[MITOEAGLE_network#Members_in_the_MITOEAGLE_network |List of MITOEAGLE mentors]]<br />
<br />
== Registration ==<br />
<br />
<br />
'''MiP''school'' registration Early Career Investigators and students'''<br />
::::» [[Media:Registration form Training School MITOEAGLE student.pdf| ECI and students registration]]<br />
<br />
::::» [[Media:Registration form Training School MITOEAGLE.pdf|Regular registration ]]<br />
<br />
<br />
'''MITOEAGLE Workshop WG1-4'''<br />
::::» [[Media:Registration_form_MITOEAGLE_Workshop_WG1-4.pdf|Registration form]]<br />
:::: Please send the completed form to [mailto:society@mitophysiology.org society@mitophysiology.org]<br />
<br />
<br />
== Travel info ==<br />
:::: How to get there? Find out more: [[MiPschool_Obergurgl_2017_Travel info|Travel info]]<br />
<br />
<br />
<br />
==Funding ==<br />
<br />
::::* [[COST Action MITOEAGLE |COST Action CA15203 Mitochondrial fitness mapping - MITOEAGLE]]<br />
<br />
[[Image:Tirol Logo Standortagentur.jpg|thumb|The project MitoFit is funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.|left| 100px]]<br />
[[Image:Medizinische-Uni-Innsbruck-Logo.gif|100px| Medical University Innsbruck]]<br />
[[Image:MitoFit.jpg|80px|link=http://www.mitofit.org/index.php/K-Regio MitoFit|K-Regio MitoFit]]<br />
<br />
<br />
<br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
<br />
<br />
{{MITOEAGLE banner}}<br />
{{Labeling<br />
|additional=2017, ORO, MitoFit, MITOEAGLE, Next<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=MiPschool_Obergurgl_2017&diff=135294MiPschool Obergurgl 20172017-05-08T12:52:38Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MiP header page name}}<br />
{{Publication<br />
|title=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]]<br />
[[Image:MiPsocietyLOGO.JPG|right|120px|link=http://www.mitophysiology.org|MiPsociety]]<br />
'''Obergurgl AT''', 2017 Jul 23-30. '''10<sup>th</sup> MiP''school'' 2017 MITOEAGLE and MITOEAGLE Workshop WG1-4.<br />
|info=<br />
|authors=Mitochondrial Physiology Society<br />
|year=2017-07-23<br />
|journal=MitoGlobal<br />
|abstract='''2017 Jul 23-30, Obergurgl, AT.''' <br />
|mipnetlab=<br />
}}<br />
[[File:MUI Logo rz rgb.jpg|150px|right|link=https://www.i-med.ac.at |Medical University Innsbruck]]<br />
[[File:Logo_age_reg.jpg|150px|right|link=https://www.uibk.ac.at/forschung/doktoratskollegs/index.html.de| DK Ageing and Regeneration]]<br />
[[File:Logo_Univ_Innsbruck.jpg|150px|right|link=https://www.uibk.ac.at |University of Innsbruck]]<br />
<br />
<br />
__TOC__<br />
== Date, venue, organization ==<br />
::::* 2017 July 23-30<br />
:::::* 2017 July 23-27 Training School jointly organized by the [[Mitochondrial Physiology Society]] and [[MC_MITOEAGLE_e-VOTE_02#Topic_.282.29_Training_School_in_Obergurgl.2C_Tyrol.2C_AT_in_July_2017 |COST Action MITOEAGLE]]<br />
:::::* 2017 July 27-30 MITOEAGLE Workshop: [[MITOEAGLE_Working_Groups|WG1-4]]<br />
::::* [http://www.uz-obergurgl.at University Centre Obergurgl], Tyrol, Austria<br />
<br />
<br />
== Programme ==<br />
<br />
=== Programme structure ===<br />
::::* '''2017-07-23, Day 1:''' Arrival<br />
::::* '''2017-07-24 to 26, Day 2-4: Training school MiP''society'' and MITOEAGLE''' <br />
::::** Day 2-3: Intoductory lectures on the mitochondrial electron transfer system (ETS), coupling of electron transfer to proton translocation and phosphorylation (OXPHOS), mitochondrial pathways, respiratory protocols for diagnosis of mitochondrial function, mitochondrial respiratory control in health and disease.<br />
::::** Day 4: Scientific lectures and student presentations<br />
::::* '''2017-07-27, Day 5:''' Departure Training school, arrival MITOEAGLE workshop, relaxing day for overall-participants<br />
::::* '''2017-07-28 to 29, Day 6-7: MITOEAGLE Workshop: WG1-4'''<br />
::::** '''WG1: Harmonization of nomenclature on mitochondrial respiratory states and control parameters:''' Status of the ''consensus paper'' on a consistent terminology on mitochondrial physiology and bioenergetics; recommendations for the use of a common terminology in mitochondrial physiological research as a milestone towards unification of concepts and nomenclature.<br />
::::** '''WG1: Respirometric reference protocols:''' (i) Standard format for presenting substrate-uncoupler-inhibitor titration (SUIT) protocols for a ‘library of protocols’ applied in mitochondrial respiratory physiology; (ii) criteria for selecting and recommending reference protocols; (iii) documentation of the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating SUIT protocols (compare: Maelstrom Research program).<br />
::::** '''WG2-4: Data repositories'''<br />
::::* '''2017-07-30, Day 8:''' Departure MITOEAGLE<br />
<br />
=== Preliminary programme MiP''school'' (Jul 23-27) ===<br />
<br />
:::: This MiP''school'' will focus on basic concepts on mitochondrial respiratory states and applications of substrate-uncoupler-inhibitor titration (SUIT) protocols. <br />
<br />
****: '''2017-07-24'''<br />
:::: '''Session A''' (morning) '''Introduction: coupling control in oxidative phosphorylation'''<br />
::::::* A1. The electron transfer system – mitochondrial pathways from fuel substrates to oxygen.<br />
::::::* A2. Coupling of the phosphorylation system to electron transfer and respiratory coupling control.<br />
::::::* A3. From Einstein's diffusion equation to Mitchell’s chemiosmotic equation: rates and states in the bioenergetics of oxidative phosphorylation.<br />
::::::* A4. Measurement of coupling in intact cells and mitochondrial preparations: biochemical coupling efficiency and ATP/O2 ratios.<br />
<br />
:::: '''Session B''' (afternoon) '''Posters and selected abstracts'''<br />
::::::* B1. Poster session.<br />
::::::* B2: Selected abstracts: measurement of coupling control in intact cells and mitochondrial preparations - open discussion.<br />
::::::* B3. Towards a data base on coupling control in intact cells.<br />
<br />
****: '''2017-07-25'''<br />
:::: '''Session C''' (morning) '''Introduction: from experimental design to data analysis'''<br />
::::::* C1. Respiratory pathway control in mitochondrial preparations.<br />
::::::* C2. Substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles.<br />
::::::* C3. Normalization of respiratory flux and flow.<br />
::::::* C4. Respiratory control ratios and control factors.<br />
<br />
:::: '''Session D''' (afternoon) '''Posters and selected abstracts'''<br />
::::::* D1. Selected abstracts: applications of SUIT protocols – open discussion.<br />
::::::* D2. SUIT protocols: design and limitations.<br />
::::::* D3. Towards a data base on mitochondrial respiratory control: diagnostic approaches and comparative mitochondrial physiology.<br />
<br />
****: '''2017-07-26'''<br />
:::: '''Session E''' (morning) '''Mitochondrial physiology: biomedical applications''' (possible titles, depending on keynote lecturers/selected abstracts)<br />
::::::* E1. The challenges of functional mitochondrial diagnosis.<br />
::::::* E2. Mitochondrial fitness in skeletal muscle ([[Garcia-Roves PM |Pablo Garcia-Roves]], University of Barcelona, ES).<br />
::::::* E3. Scope and limitations of functional mitochondrial diagnosis in blood cells.<br />
::::::* E4. Mitochondrial respiratory function and neuromuscular disease.<br />
::::::* E5. Mitochondrial function and dysfunction in cancer ([[Porter RK |Richard K Porter]], Trinity College Dublin, IE).<br />
::::::* E6. Ischemia-reperfusion injury.<br />
::::::* E7. The metabolic syndrome ([[Lee HK |Hong Kyu Lee]], Eulji University College of Medicine, Seoul, KR).<br />
::::::* E8. Mitochondrial function in aging and regeneration ([[Duerr PJ |Pidder-Jansen Duerr]], University of Innsbruck, AT). <br />
<br />
:::: '''Session F''' (afternoon) '''Comparative mitochondrial physiology''' (possible titles, depending on keynote lecturers/selected abstracts)<br />
::::::* F1. Mitochondrial physiology in cell models versus primary tissue.<br />
::::::* F2. Mitochondrial physiology studies in mouse models.<br />
::::::* F3. Mitochondrial vertrebrate physiology – extreme performers.<br />
::::::* F5. Mitochondrial physiology of and beyond established animal models.<br />
::::::* F6. Mitochondrial physiology in plants and microbes.<br />
::::::* F7. Mitochondrial physiology and evolution.<br />
<br />
:::: '''Session G''' (evening) '''MITOEAGLE Early Career Investigators forum''' <br />
::::::* [[MITOEAGLE Early Career Investigators]]<br />
<br />
<br />
<br />
=== Preliminary programme MITOEAGLE Workshop WG1-4 (Jul 27-30) ===<br />
:::: currently in progress<br />
<br />
<br />
<br />
== Abstracts ==<br />
<br />
:::: Abstract submission: until '''May 31, 2017.'''<br />
:::: The abstract title has to be included on the registration form.<br />
<br />
:::* '''Categories''': Early career investigators (ECI) and students are invited to submit abstracts in four alternative categories and apply with their submission for a [[MiPschool_Obergurgl_2017#Support|MITOEAGLE Scholarship]]:<br />
<br />
::::# SUIT protocols: basic concepts. - » [[MitoPedia: SUIT]] <br />
::::# SUIT protocols: various applications with intact cells and mt-preparations.<br />
::::# Discuss definitions and controversies for a selected term on respiratory states and respiratory control ratios. - » [[MitoPedia: Respiratory states]], [[MitoPedia: Respiratory control ratios]]<br />
::::# Present your abstract as a definition of a term in the context of mitochondrial physiology that is missing in MitoPedia. - » [[MitoPedia]]<br />
<br />
:::* '''Format'''<br />
:::::: [[Abstract format]]<br />
:::::: '''Example''': [[Doerrier 2017 Abstract MITOEAGLE Barcelona]]<br />
<br />
<br />
:::* '''Abstract submission and review for MITOEAGLE Scholarships'''<br />
::::1. '''Eligibility:''' Early career investigators and students from all COST countries and International Partner Countries can apply - [[MITOEAGLE network]]. <br />
::::2. You need to select a 'Mentor' who will act as the contact to review your abstract and recommend you as a recipient of the MITOEAGLE scholarship.<br />
::::3. Check the [[MiPschool_Obergurgl_2017_Mentors|MITOEAGLE mentors website]] for the Training School, and select a 'Mentor'. <br />
::::4. Click on the highlighted name of the mentor for further details.<br />
::::::* Each mentor has two slots available. You can see in the column ''Booked'' whether the mentor is already booked or not. <br />
::::::*If you see that your preference no. 1 mentor already has one 'student', you might consider going for another mentor.<br />
::::5. Prepare your abstract in the format given above.<br />
::::::* Add the name of your selected mentor as a final section of your abstract.<br />
::::::* Cover letter: Provide a brief explanation to your selected mentor in a form of a cover letter.<br />
::::6. Send an Email to your mentor (and in Cc to mitoeagle@i-med.ac.at). Mention MITOEAGLE mentor in the subject line of your Email. Attach your abstract as a MS Word file and the cover letter.<br />
::::7. Experimental abstracts will be reviewed and accepted entirely independent of the experimental platform used in the study, and a wide variety of experimental approaches is highly welcome.<br />
<br />
::::» '''Notificiation of MITOEAGLE scholarships: 2017-06-10.'''<br />
<br />
<br />
== Support ==<br />
<br />
=== MITOEAGLE scholarships for Early Career Investigators and students ===<br />
<br />
::::* '''Submitted abstracts provide the basis for allocation of MITOEAGLE scholarships.<br />
::::* '''Proposed deadline: 2017-May-31'''.<br />
::::* '''42 basic scholarships''' will cover the local costs (accommodation, meals and registration). <br />
::::* Students from Inclusiveness Target Countries are eligible for '''extended scholarships''' covering travel costs in addition to the basic scholarship.<br />
<br />
::::* Abstracts submitted with data suitable for the data bases [[MITOEAGLE_Working_Groups|Working Groups 2, 3 and 4]] or [[WG1 MITOEAGLE protocols, terminology, documentation |Working Group 1]] are selected as a basis of additional support for joining the MITOEAGLE Workshop WG1-4 (Jul 27-30).<br />
::::* MITOEAGLE Working Group participants are supported (as far as possible) to join the Training School to be better prepared for the collaboration in the WG meeting, particularly the group working on the terminology review on respiratory states.<br />
<br />
::::» '''Notificiation of MITOEAGLE scholarships: 2017-06-10.'''<br />
<br />
<br />
<br />
<br />
[[File:Questions.jpg|left|60px]] <br />
::::» For further questions, please contact the '''MITOEAGLE representatives of the training schools''': <br />
:::::: [[Schlattner U |Uwe Schlattner]]<br />
:::::: [[Engin AB |Ayse Basak Engin]]<br />
::::» Further details on mentorship: consult [[MITOEAGLE Early Career Investigators]]<br />
::::» [[MITOEAGLE_network#Members_in_the_MITOEAGLE_network |List of MITOEAGLE mentors]]<br />
<br />
== Registration ==<br />
<br />
<br />
'''MiP''school'' registration Early Career Investigators and students'''<br />
::::» [[Media:Registration form Training School MITOEAGLE student.pdf| ECI and students registration]]<br />
<br />
::::» [[Media:Registration form Training School MITOEAGLE.pdf|Regular registration ]]<br />
<br />
<br />
'''MITOEAGLE Workshop WG1-4'''<br />
::::» [[Media:Registration_form_MITOEAGLE_Workshop_WG1-4.pdf|Registration form]]<br />
:::: Please send the completed form to [[mailto:society@mitophysiology.org society@mitophysiology.org]]<br />
<br />
<br />
== Travel info ==<br />
:::: How to get there? Find out more: [[MiPschool_Obergurgl_2017_Travel info|Travel info]]<br />
<br />
<br />
<br />
==Funding ==<br />
<br />
::::* [[COST Action MITOEAGLE |COST Action CA15203 Mitochondrial fitness mapping - MITOEAGLE]]<br />
<br />
[[Image:Tirol Logo Standortagentur.jpg|thumb|The project MitoFit is funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.|left| 100px]]<br />
[[Image:Medizinische-Uni-Innsbruck-Logo.gif|100px| Medical University Innsbruck]]<br />
[[Image:MitoFit.jpg|80px|link=http://www.mitofit.org/index.php/K-Regio MitoFit|K-Regio MitoFit]]<br />
<br />
<br />
<br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
<br />
<br />
{{MITOEAGLE banner}}<br />
{{Labeling<br />
|additional=2017, ORO, MitoFit, MITOEAGLE, Next<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=MiPschool_Obergurgl_2017&diff=135282MiPschool Obergurgl 20172017-05-08T12:19:45Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MiP header page name}}<br />
{{Publication<br />
|title=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]]<br />
[[Image:MiPsocietyLOGO.JPG|right|120px|link=http://www.mitophysiology.org|MiPsociety]]<br />
'''Obergurgl AT''', 2017 Jul 23-30. '''10<sup>th</sup> MiP''school'' 2017 MITOEAGLE and MITOEAGLE Workshop WG1-4.<br />
|info=<br />
|authors=Mitochondrial Physiology Society<br />
|year=2017-07-23<br />
|journal=MitoGlobal<br />
|abstract='''2017 Jul 23-30, Obergurgl, AT.''' <br />
|mipnetlab=<br />
}}<br />
[[File:MUI Logo rz rgb.jpg|150px|right|link=https://www.i-med.ac.at |Medical University Innsbruck]]<br />
[[File:Logo_age_reg.jpg|150px|right|link=https://www.uibk.ac.at/forschung/doktoratskollegs/index.html.de| DK Ageing and Regeneration]]<br />
[[File:Logo_Univ_Innsbruck.jpg|150px|right|link=https://www.uibk.ac.at |University of Innsbruck]]<br />
<br />
<br />
__TOC__<br />
== Date, venue, organization ==<br />
::::* 2017 July 23-30<br />
:::::* 2017 July 23-27 Training School jointly organized by the [[Mitochondrial Physiology Society]] and [[MC_MITOEAGLE_e-VOTE_02#Topic_.282.29_Training_School_in_Obergurgl.2C_Tyrol.2C_AT_in_July_2017 |COST Action MITOEAGLE]]<br />
:::::* 2017 July 27-30 MITOEAGLE Workshop: [[MITOEAGLE_Working_Groups|WG1-4]]<br />
::::* [http://www.uz-obergurgl.at University Centre Obergurgl], Tyrol, Austria<br />
<br />
<br />
== Programme ==<br />
<br />
=== Programme structure ===<br />
::::* '''2017-07-23, Day 1:''' Arrival<br />
::::* '''2017-07-24 to 26, Day 2-4: Training school MiP''society'' and MITOEAGLE''' <br />
::::** Day 2-3: Intoductory lectures on the mitochondrial electron transfer system (ETS), coupling of electron transfer to proton translocation and phosphorylation (OXPHOS), mitochondrial pathways, respiratory protocols for diagnosis of mitochondrial function, mitochondrial respiratory control in health and disease.<br />
::::** Day 4: Scientific lectures and student presentations<br />
::::* '''2017-07-27, Day 5:''' Departure Training school, arrival MITOEAGLE workshop, relaxing day for overall-participants<br />
::::* '''2017-07-28 to 29, Day 6-7: MITOEAGLE Workshop: WG1-4'''<br />
::::** '''WG1: Harmonization of nomenclature on mitochondrial respiratory states and control parameters:''' Status of the ''consensus paper'' on a consistent terminology on mitochondrial physiology and bioenergetics; recommendations for the use of a common terminology in mitochondrial physiological research as a milestone towards unification of concepts and nomenclature.<br />
::::** '''WG1: Respirometric reference protocols:''' (i) Standard format for presenting substrate-uncoupler-inhibitor titration (SUIT) protocols for a ‘library of protocols’ applied in mitochondrial respiratory physiology; (ii) criteria for selecting and recommending reference protocols; (iii) documentation of the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating SUIT protocols (compare: Maelstrom Research program).<br />
::::** '''WG2-4: Data repositories'''<br />
::::* '''2017-07-30, Day 8:''' Departure MITOEAGLE<br />
<br />
=== Preliminary programme MiP''school'' (Jul 23-27) ===<br />
<br />
:::: This MiP''school'' will focus on basic concepts on mitochondrial respiratory states and applications of substrate-uncoupler-inhibitor titration (SUIT) protocols. <br />
<br />
****: '''2017-07-24'''<br />
:::: '''Session A''' (morning) '''Introduction: coupling control in oxidative phosphorylation'''<br />
::::::* A1. The electron transfer system – mitochondrial pathways from fuel substrates to oxygen.<br />
::::::* A2. Coupling of the phosphorylation system to electron transfer and respiratory coupling control.<br />
::::::* A3. From Einstein's diffusion equation to Mitchell’s chemiosmotic equation: rates and states in the bioenergetics of oxidative phosphorylation.<br />
::::::* A4. Measurement of coupling in intact cells and mitochondrial preparations: biochemical coupling efficiency and ATP/O2 ratios.<br />
<br />
:::: '''Session B''' (afternoon) '''Posters and selected abstracts'''<br />
::::::* B1. Poster session.<br />
::::::* B2: Selected abstracts: measurement of coupling control in intact cells and mitochondrial preparations - open discussion.<br />
::::::* B3. Towards a data base on coupling control in intact cells.<br />
<br />
****: '''2017-07-25'''<br />
:::: '''Session C''' (morning) '''Introduction: from experimental design to data analysis'''<br />
::::::* C1. Respiratory pathway control in mitochondrial preparations.<br />
::::::* C2. Substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles.<br />
::::::* C3. Normalization of respiratory flux and flow.<br />
::::::* C4. Respiratory control ratios and control factors.<br />
<br />
:::: '''Session D''' (afternoon) '''Posters and selected abstracts'''<br />
::::::* D1. Selected abstracts: applications of SUIT protocols – open discussion.<br />
::::::* D2. SUIT protocols: design and limitations.<br />
::::::* D3. Towards a data base on mitochondrial respiratory control: diagnostic approaches and comparative mitochondrial physiology.<br />
<br />
****: '''2017-07-26'''<br />
:::: '''Session E''' (morning) '''Mitochondrial physiology: biomedical applications''' (possible titles, depending on keynote lecturers/selected abstracts)<br />
::::::* E1. The challenges of functional mitochondrial diagnosis.<br />
::::::* E2. Mitochondrial fitness in skeletal muscle ([[Garcia-Roves PM |Pablo Garcia-Roves]], University of Barcelona, ES).<br />
::::::* E3. Scope and limitations of functional mitochondrial diagnosis in blood cells.<br />
::::::* E4. Mitochondrial respiratory function and neuromuscular disease.<br />
::::::* E5. Mitochondrial function and dysfunction in cancer ([[Porter RK |Richard K Porter]], Trinity College Dublin, IE).<br />
::::::* E6. Ischemia-reperfusion injury.<br />
::::::* E7. The metabolic syndrome ([[Lee HK |Hong Kyu Lee]], Eulji University College of Medicine, Seoul, KR).<br />
::::::* E8. Mitochondrial function in aging and regeneration ([[Duerr PJ |Pidder-Jansen Duerr]], University of Innsbruck, AT). <br />
<br />
:::: '''Session F''' (afternoon) '''Comparative mitochondrial physiology''' (possible titles, depending on keynote lecturers/selected abstracts)<br />
::::::* F1. Mitochondrial physiology in cell models versus primary tissue.<br />
::::::* F2. Mitochondrial physiology studies in mouse models.<br />
::::::* F3. Mitochondrial vertrebrate physiology – extreme performers.<br />
::::::* F5. Mitochondrial physiology of and beyond established animal models.<br />
::::::* F6. Mitochondrial physiology in plants and microbes.<br />
::::::* F7. Mitochondrial physiology and evolution.<br />
<br />
:::: '''Session G''' (evening) '''MITOEAGLE Early Career Investigators forum''' <br />
::::::* [[MITOEAGLE Early Career Investigators]]<br />
<br />
<br />
<br />
=== Preliminary programme MITOEAGLE Workshop WG1-4 (Jul 27-30) ===<br />
:::: currently in progress<br />
<br />
<br />
<br />
== Abstracts ==<br />
<br />
:::: Abstract submission: until '''May 31, 2017.'''<br />
:::: The abstract title has to be included on the registration form.<br />
<br />
:::* '''Categories''': Early career investigators (ECI) and students are invited to submit abstracts in four alternative categories and apply with their submission for a [[MiPschool_Obergurgl_2017#Support|MITOEAGLE Scholarship]]:<br />
<br />
::::# SUIT protocols: basic concepts. - » [[MitoPedia: SUIT]] <br />
::::# SUIT protocols: various applications with intact cells and mt-preparations.<br />
::::# Discuss definitions and controversies for a selected term on respiratory states and respiratory control ratios. - » [[MitoPedia: Respiratory states]], [[MitoPedia: Respiratory control ratios]]<br />
::::# Present your abstract as a definition of a term in the context of mitochondrial physiology that is missing in MitoPedia. - » [[MitoPedia]]<br />
<br />
:::* '''Format'''<br />
:::::: [[Abstract format]]<br />
:::::: '''Example''': [[Doerrier 2017 Abstract MITOEAGLE Barcelona]]<br />
<br />
<br />
:::* '''Abstract submission and review for MITOEAGLE Scholarships'''<br />
::::1. '''Eligibility:''' Early career investigators and students from all COST countries and International Partner Countries can apply - [[MITOEAGLE network]]. <br />
::::2. You need to select a 'Mentor' who will act as the contact to review your abstract and recommend you as a recipient of the MITOEAGLE scholarship.<br />
::::3. Check the [[MiPschool_Obergurgl_2017_Mentors|MITOEAGLE mentors website]] for the Training School, and select a 'Mentor'. <br />
::::4. Click on the highlighted name of the mentor for further details.<br />
::::::* Each mentor has two slots available. You can see in the column ''Booked'' whether the mentor is already booked or not. <br />
::::::*If you see that your preference no. 1 mentor already has one 'student', you might consider going for another mentor.<br />
::::5. Prepare your abstract in the format given above.<br />
::::::* Add the name of your selected mentor as a final section of your abstract.<br />
::::::* Cover letter: Provide a brief explanation to your selected mentor in a form of a cover letter.<br />
::::6. Send an Email to your mentor (and in Cc to mitoeagle@i-med.ac.at). Mention MITOEAGLE mentor in the subject line of your Email. Attach your abstract as a MS Word file and the cover letter.<br />
::::7. Experimental abstracts will be reviewed and accepted entirely independent of the experimental platform used in the study, and a wide variety of experimental approaches is highly welcome.<br />
<br />
::::» '''Notificiation of MITOEAGLE scholarships: 2017-06-10.'''<br />
<br />
<br />
== Support ==<br />
<br />
=== MITOEAGLE scholarships for Early Career Investigators and students ===<br />
<br />
::::* '''Submitted abstracts provide the basis for allocation of MITOEAGLE scholarships.<br />
::::* '''Proposed deadline: 2017-May-31'''.<br />
::::* '''42 basic scholarships''' will cover the local costs (accommodation, meals and registration). <br />
::::* Students from Inclusiveness Target Countries are eligible for '''extended scholarships''' covering travel costs in addition to the basic scholarship.<br />
<br />
::::* Abstracts submitted with data suitable for the data bases [[MITOEAGLE_Working_Groups|Working Groups 2, 3 and 4]] or [[WG1 MITOEAGLE protocols, terminology, documentation |Working Group 1]] are selected as a basis of additional support for joining the MITOEAGLE Workshop WG1-4 (Jul 27-30).<br />
::::* MITOEAGLE Working Group participants are supported (as far as possible) to join the Training School to be better prepared for the collaboration in the WG meeting, particularly the group working on the terminology review on respiratory states.<br />
<br />
::::» '''Notificiation of MITOEAGLE scholarships: 2017-06-10.'''<br />
<br />
<br />
<br />
<br />
[[File:Questions.jpg|left|60px]] <br />
::::» For further questions, please contact the '''MITOEAGLE representatives of the training schools''': <br />
:::::: [[Schlattner U |Uwe Schlattner]]<br />
:::::: [[Engin AB |Ayse Basak Engin]]<br />
::::» Further details on mentorship: consult [[MITOEAGLE Early Career Investigators]]<br />
::::» [[MITOEAGLE_network#Members_in_the_MITOEAGLE_network |List of MITOEAGLE mentors]]<br />
<br />
== Registration ==<br />
<br />
<br />
'''MiP''school'' registration Early Career Investigators and students'''<br />
::::» [[Media:Registration form Training School MITOEAGLE student.pdf| ECI and students registration]]<br />
<br />
::::» [[Media:Registration form Training School MITOEAGLE.pdf|Regular registration ]]<br />
<br />
<br />
'''MITOEAGLE Workshop WG1-4'''<br />
::::» [[Media:Registration_form_MITOEAGLE_Workshop_WG1-4.pdf|Registration form]]<br />
<br />
<br />
== Travel info ==<br />
:::: How to get there? Find out more: [[MiPschool_Obergurgl_2017_Travel info|Travel info]]<br />
<br />
<br />
<br />
==Funding ==<br />
<br />
::::* [[COST Action MITOEAGLE |COST Action CA15203 Mitochondrial fitness mapping - MITOEAGLE]]<br />
<br />
[[Image:Tirol Logo Standortagentur.jpg|thumb|The project MitoFit is funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.|left| 100px]]<br />
[[Image:Medizinische-Uni-Innsbruck-Logo.gif|100px| Medical University Innsbruck]]<br />
[[Image:MitoFit.jpg|80px|link=http://www.mitofit.org/index.php/K-Regio MitoFit|K-Regio MitoFit]]<br />
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<br />
<br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
<br />
<br />
{{MITOEAGLE banner}}<br />
{{Labeling<br />
|additional=2017, ORO, MitoFit, MITOEAGLE, Next<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Monte_Rosa&diff=135252Monte Rosa2017-05-08T06:39:46Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{OROBOROS header page name}}<br />
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<br />
[[Image:The world as a laboratory.jpg|right|180px|link=http://wiki.oroboros.at/index.php/The_world_as_a_laboratory|Science and adventure]]<br />
== Project Monte Rosa 2004 ==<br />
<br />
[[File:Rosa.jpg|240px|left]]<br />
:::[[CH_Zurich_Lundby_C|Carsten Lundby]], from The Copenhagen Muscle Research Centre (Rigshospitalet, Copenhagen, Denmark) was the project leader of the international expedition to the Margherita Hut on Monte Rosa. [[SE Stockholm Boushel RC|Robert Boushel]] and Cindy Wright-Paradis (Dept. of Exercise Science, Concordia University, Montreal, Canada) and [[Gnaiger_E|Erich Gnaiger]] (Dept. General and Transplant Surgery, Innsbruck Medical University, Austria) collaborated within this high-altitude physiology project to study mechanisms of acclimation in mitochondrial respiratory function in small muscle biopsies. On Monte Rosa, two OROBOROS O2k were applied at high altitude, to perform high-resolution respirometric experiments under the extreme conditions close to the peak of the second-highest mountain of the Alps after Mont Blanc. Biopsies were taken by Jose Calbet (Las Palmas, ES) and Kirsten Moller (Copenhagen, DK). The expedition started on 21 August 2004 and returned on 4 September 2004. <br />
<br />
<br />
::::* Jacobs RA, Boushel RC, Wright-Paradis C, Calbet JA, Robach P, Gnaiger E, Lundby C (2012) Mitochondrial function in human skeletal muscle following high altitude exposure. Exp Physiol 98:245-55. - [[Jacobs_2013_Exp_Physiol |»Bioblast link«]]<br />
<br />
::::* [http://www.mitophysiology.org/index.php?gnaigere Erich Gnaiger] (Innsbruck AT) High-resolution respirometry in small biopsies of human muscle: Correlations with body mass index and age. MiP2005:14-5.<br />
<br />
::::* [http://www.mitophysiology.org/index.php?wright-paradisc Cindy Wright-Paradis] (Montreal CA) Mitochondrial respiratory function in human skeletal muscle fibers studied at high altitude. MiP2005:28-9.<br />
<br />
<br />
::::'''Monte Rosa Project 2004 ([[MiPNet09.14 Monte Rosa Expedition 2004|Monte Rosa Expedition]], [http://bioblast.at/images/2/25/MiPNet09.14_Monte_Rosa_Expedition_2004.pdf download pdf]; 2004-10-24)'''<br />
<br />
<br />
<br />
<br />
== Over 100 years ago: Durig and Zuntz 1904 ==<br />
<br />
[[File:Rosa2.jpg|250px|right|Arnold Durig (1872-1961)]]<br />
::::More than 100 years ago (Durig and Zunz, 1904), Arnold Durig (studies of medicine in Innsbruck, collaboration with N. Zuntz in Berlin, professor of physiology in Vienna, Austria) and Nathan Zuntz (Berlin, Germany) described how they "ascended to the Col d'Olen (2900 meters), and, having remained there for a week, passed upward to a hut (4560 meters) constructed near the summit of Monte Rosa ... They lived in this hut two weeks and a half. The height of the barometer was 443 millimeters, which indicates a quantity of oxygen amounting to 12.2 per cent. of an atmosphere. On the Col d'Olen there was no increase in their metabolism when they were resting, and there was no increase in the requirement of energy necessary to accomplish one kilogrammeter of work. ... At the higher level, ... the resting metabolism increased at once and permanently to the extent of 15 per cent. ... The increased metabolism was not due to cold, for it was present when the individual was in a warm bed in the hut." (Lusk, 1928).<br />
<br />
::::* Durig A, Zuntz N (1904) Beiträge zur Physiologie des Menschen im Hochgebirge. Archiv für Anatomie und Physiologie. Physiologische Abteilung. Supplement:417-56.<br />
<br />
::::* Burtscher M, Gnaiger E, Burtscher J, Nachbauer W, Brugger A (2012) Arnold Durig (1872-1961): life and work. An Austrian pioneer in exercise and high altitude physiology. High Alt Med Biol 13:224-31. - [[Burtscher_2012_High_Alt_Med_Biol |»Bioblast link«]]<br />
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<br />
== Capanna Regina Margherita (4559 m) ==<br />
<br />
[[File:Rosa3.jpg|250px|right]]<br />
::::The hut near the summit of Monte Rosa is the famous Capanna Regina Margherita constructed in 1893 at an altitude of 4559 m on the Punta Gnifetti by the Club Alpino Italiano. The CRM was officially opened on 18. August 1893 in the presence of the Italian Queen, who supported and sponsored a scientific laboratory at this hut which bears her name. It was declared as an international laboratory in 1901 at the Physiological Conference in Turino. After years of limited use and deterioration, the new Capanna Regina Margherita was built at the same location in 1979.<br />
<br />
<br />
'''Literature'''<br />
::::* Lusk G (1928) The elements of the science of nutrition. Sauners Company, Philadelphia and London:584-5.<br />
::::* Simons E, Oelz O (2001) Kopfwehberge. Eine Geschichte der Höhenmedizin. AS Verlag, Zürich:231 pp.</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Fatty_Acid_Oxidation_O2k-Network_Discussion_Forum&diff=135251Fatty Acid Oxidation O2k-Network Discussion Forum2017-05-08T06:35:32Z<p>Bitschnau Barbara: Redirected page to Talk:Fatty acid oxidation</p>
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<div>#REDIRECT [[Talk:Fatty acid oxidation]]</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Fatty_Acid_Oxidation_O2k-Network_Discussion_Forum&diff=135250Fatty Acid Oxidation O2k-Network Discussion Forum2017-05-08T06:35:22Z<p>Bitschnau Barbara: </p>
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<div>#REDIRECCT [[Talk:Fatty acid oxidation]]</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Fatty_Acid_Oxidation_O2k-Network_Discussion_Forum&diff=135249Fatty Acid Oxidation O2k-Network Discussion Forum2017-05-08T06:34:35Z<p>Bitschnau Barbara: Blanked the page</p>
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<div></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Fatty_Acid_Oxidation_O2k-Network_Discussion_Forum&diff=135248Fatty Acid Oxidation O2k-Network Discussion Forum2017-05-08T06:34:02Z<p>Bitschnau Barbara: </p>
<hr />
<div>#REDIRECT [Talk:Fatty acid oxidation]</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Fatty_Acid_Oxidation_O2k-Network_Discussion_Forum&diff=135247Fatty Acid Oxidation O2k-Network Discussion Forum2017-05-08T06:33:07Z<p>Bitschnau Barbara: Created page with "#REDIRECT [Talk:Fatty_acid_oxidation]"</p>
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<div>#REDIRECT [Talk:Fatty_acid_oxidation]</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=MiPschool_Obergurgl_2017&diff=135231MiPschool Obergurgl 20172017-05-05T12:29:21Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MiP header page name}}<br />
{{Publication<br />
|title=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]]<br />
[[Image:MiPsocietyLOGO.JPG|right|120px|link=http://www.mitophysiology.org|MiPsociety]]<br />
'''Obergurgl AT''', 2017 Jul 23-30. '''10<sup>th</sup> MiP''school'' 2017 MITOEAGLE and MITOEAGLE Workshop WG1-4.<br />
|info=<br />
|authors=Mitochondrial Physiology Society<br />
|year=2017-07-23<br />
|journal=MitoGlobal<br />
|abstract='''2017 Jul 23-30, Obergurgl, AT.''' <br />
|mipnetlab=<br />
}}<br />
[[File:MUI Logo rz rgb.jpg|200px|right|link=https://www.i-med.ac.at |Medical University Innsbruck]]<br />
[[File:Logo_age_reg.jpg|100px|right|link=https://www.uibk.ac.at/forschung/doktoratskollegs/index.html.de| DK Ageing and Regeneration]]<br />
[[File:Logo_Univ_Innsbruck.jpg|150px|right|link=https://www.uibk.ac.at |University of Innsbruck]]<br />
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<br />
__TOC__<br />
== Date, venue, organization ==<br />
::::* 2017 July 23-30<br />
:::::* 2017 July 23-27 Training School jointly organized by the [[Mitochondrial Physiology Society]] and [[MC_MITOEAGLE_e-VOTE_02#Topic_.282.29_Training_School_in_Obergurgl.2C_Tyrol.2C_AT_in_July_2017 |COST Action MITOEAGLE]]<br />
:::::* 2017 July 27-30 MITOEAGLE Workshop: [[MITOEAGLE_Working_Groups|WG1-4]]<br />
::::* [http://www.uz-obergurgl.at University Centre Obergurgl], Tyrol, Austria<br />
<br />
<br />
== Programme ==<br />
<br />
=== Programme structure ===<br />
::::* '''2017-07-23, Day 1:''' Arrival<br />
::::* '''2017-07-24 to 26, Day 2-4: Training school MiP''society'' and MITOEAGLE''' <br />
::::** Day 2-3: Intoductory lectures on the mitochondrial electron transfer system (ETS), coupling of electron transfer to proton translocation and phosphorylation (OXPHOS), mitochondrial pathways, respiratory protocols for diagnosis of mitochondrial function, mitochondrial respiratory control in health and disease.<br />
::::** Day 4: Scientific lectures and student presentations<br />
::::* '''2017-07-27, Day 5:''' Departure Training school, arrival MITOEAGLE workshop, relaxing day for overall-participants<br />
::::* '''2017-07-28 to 29, Day 6-7: MITOEAGLE Workshop: WG1-4'''<br />
::::** '''WG1: Harmonization of nomenclature on mitochondrial respiratory states and control parameters:''' Status of the ''consensus paper'' on a consistent terminology on mitochondrial physiology and bioenergetics; recommendations for the use of a common terminology in mitochondrial physiological research as a milestone towards unification of concepts and nomenclature.<br />
::::** '''WG1: Respirometric reference protocols:''' (i) Standard format for presenting substrate-uncoupler-inhibitor titration (SUIT) protocols for a ‘library of protocols’ applied in mitochondrial respiratory physiology; (ii) criteria for selecting and recommending reference protocols; (iii) documentation of the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating SUIT protocols (compare: Maelstrom Research program).<br />
::::** '''WG2-4: Data repositories'''<br />
::::* '''2017-07-30, Day 8:''' Departure MITOEAGLE<br />
<br />
=== Preliminary programme MiP''school'' (Jul 23-27) ===<br />
<br />
:::: This MiP''school'' will focus on basic concepts on mitochondrial respiratory states and applications of substrate-uncoupler-inhibitor titration (SUIT) protocols. <br />
<br />
****: '''2017-07-24'''<br />
:::: '''Session A''' (morning) '''Introduction: coupling control in oxidative phosphorylation'''<br />
::::::* A1. The electron transfer system – mitochondrial pathways from fuel substrates to oxygen.<br />
::::::* A2. Coupling of the phosphorylation system to electron transfer and respiratory coupling control.<br />
::::::* A3. From Einstein's diffusion equation to Mitchell’s chemiosmotic equation: rates and states in the bioenergetics of oxidative phosphorylation.<br />
::::::* A4. Measurement of coupling in intact cells and mitochondrial preparations: biochemical coupling efficiency and ATP/O2 ratios.<br />
<br />
:::: '''Session B''' (afternoon) '''Posters and selected abstracts'''<br />
::::::* B1. Poster session.<br />
::::::* B2: Selected abstracts: measurement of coupling control in intact cells and mitochondrial preparations - open discussion.<br />
::::::* B3. Towards a data base on coupling control in intact cells.<br />
<br />
****: '''2017-07-25'''<br />
:::: '''Session C''' (morning) '''Introduction: from experimental design to data analysis'''<br />
::::::* C1. Respiratory pathway control in mitochondrial preparations.<br />
::::::* C2. Substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles.<br />
::::::* C3. Normalization of respiratory flux and flow.<br />
::::::* C4. Respiratory control ratios and control factors.<br />
<br />
:::: '''Session D''' (afternoon) '''Posters and selected abstracts'''<br />
::::::* D1. Selected abstracts: applications of SUIT protocols – open discussion.<br />
::::::* D2. SUIT protocols: design and limitations.<br />
::::::* D3. Towards a data base on mitochondrial respiratory control: diagnostic approaches and comparative mitochondrial physiology.<br />
<br />
****: '''2017-07-26'''<br />
:::: '''Session E''' (morning) '''Mitochondrial physiology: biomedical applications''' (possible titles, depending on keynote lecturers/selected abstracts)<br />
::::::* E1. The challenges of functional mitochondrial diagnosis.<br />
::::::* E2. Mitochondrial fitness in skeletal muscle ([[Garcia-Roves PM |Pablo Garcia-Roves]], University of Barcelona, ES).<br />
::::::* E3. Scope and limitations of functional mitochondrial diagnosis in blood cells.<br />
::::::* E4. Mitochondrial respiratory function and neuromuscular disease.<br />
::::::* E5. Mitochondrial function and dysfunction in cancer ([[Porter RK |Richard K Porter]], Trinity College Dublin, IE).<br />
::::::* E6. Ischemia-reperfusion injury.<br />
::::::* E7. The metabolic syndrome ([[Lee HK |Hong Kyu Lee]], Eulji University College of Medicine, Seoul, KR).<br />
::::::* E8. Mitochondrial function in aging and regeneration ([[Duerr PJ |Pidder-Jansen Duerr]], University of Innsbruck, AT). <br />
<br />
:::: '''Session F''' (afternoon) '''Comparative mitochondrial physiology''' (possible titles, depending on keynote lecturers/selected abstracts)<br />
::::::* F1. Mitochondrial physiology in cell models versus primary tissue.<br />
::::::* F2. Mitochondrial physiology studies in mouse models.<br />
::::::* F3. Mitochondrial vertrebrate physiology – extreme performers.<br />
::::::* F5. Mitochondrial physiology of and beyond established animal models.<br />
::::::* F6. Mitochondrial physiology in plants and microbes.<br />
::::::* F7. Mitochondrial physiology and evolution.<br />
<br />
:::: '''Session G''' (evening) '''MITOEAGLE Early Career Investigators forum''' <br />
::::::* [[MITOEAGLE Early Career Investigators]]<br />
<br />
<br />
<br />
=== Preliminary programme MITOEAGLE Workshop WG1-4 (Jul 27-30) ===<br />
:::: currently in progress<br />
<br />
<br />
<br />
== Abstracts ==<br />
<br />
:::: Abstract submission: until '''May 31, 2017.'''<br />
:::: The abstract title has to be included on the registration form.<br />
<br />
:::* '''Categories''': Early career investigators (ECI) and students are invited to submit abstracts in four alternative categories and apply with their submission for a [[MiPschool_Obergurgl_2017#Support|MITOEAGLE Scholarship]]:<br />
<br />
::::# SUIT protocols: basic concepts. - » [[MitoPedia: SUIT]] <br />
::::# SUIT protocols: various applications with intact cells and mt-preparations.<br />
::::# Discuss definitions and controversies for a selected term on respiratory states and respiratory control ratios. - » [[MitoPedia: Respiratory states]], [[MitoPedia: Respiratory control ratios]]<br />
::::# Present your abstract as a definition of a term in the context of mitochondrial physiology that is missing in MitoPedia. - » [[MitoPedia]]<br />
<br />
:::* '''Format'''<br />
:::::: [[Abstract format]]<br />
:::::: '''Example''': [[Doerrier 2017 Abstract MITOEAGLE Barcelona]]<br />
<br />
<br />
:::* '''Abstract submission and review for MITOEAGLE Scholarships'''<br />
::::1. '''Eligibility:''' Early career investigators and students from all COST countries and International Partner Countries can apply - [[MITOEAGLE network]]. <br />
::::2. You need to select a 'Mentor' who will act as the contact to review your abstract and recommend you as a recipient of the MITOEAGLE scholarship.<br />
::::3. Check the [[MiPschool_Obergurgl_2017_Mentors|MITOEAGLE mentors website]] for the Training School, and select a 'Mentor'. <br />
::::4. Click on the highlighted name of the mentor for further details.<br />
::::::* Each mentor has two slots available. You can see in the column ''Booked'' whether the mentor is already booked or not. <br />
::::::*If you see that your preference no. 1 mentor already has one 'student', you might consider going for another mentor.<br />
::::5. Prepare your abstract in the format given above.<br />
::::::* Add the name of your selected mentor as a final section of your abstract.<br />
::::::* Cover letter: Provide a brief explanation to your selected mentor in a form of a cover letter.<br />
::::6. Send an Email to your mentor (and in Cc to mitoeagle@i-med.ac.at). Mention MITOEAGLE mentor in the subject line of your Email. Attach your abstract as a MS Word file and the cover letter.<br />
::::7. Experimental abstracts will be reviewed and accepted entirely independent of the experimental platform used in the study, and a wide variety of experimental approaches is highly welcome.<br />
<br />
::::» '''Notificiation of MITOEAGLE scholarships: 2017-06-10.'''<br />
<br />
<br />
== Support ==<br />
<br />
=== MITOEAGLE scholarships for Early Career Investigators and students ===<br />
<br />
::::* '''Submitted abstracts provide the basis for allocation of MITOEAGLE scholarships.<br />
::::* '''Proposed deadline: 2017-May-31'''.<br />
::::* '''42 basic scholarships''' will cover the local costs (accommodation, meals and registration). <br />
::::* Students from Inclusiveness Target Countries are eligible for '''extended scholarships''' covering travel costs in addition to the basic scholarship.<br />
<br />
::::* Abstracts submitted with data suitable for the data bases [[MITOEAGLE_Working_Groups|Working Groups 2, 3 and 4]] or [[WG1 MITOEAGLE protocols, terminology, documentation |Working Group 1]] are selected as a basis of additional support for joining the MITOEAGLE Workshop WG1-4 (Jul 27-30).<br />
::::* MITOEAGLE Working Group participants are supported (as far as possible) to join the Training School to be better prepared for the collaboration in the WG meeting, particularly the group working on the terminology review on respiratory states.<br />
<br />
::::» '''Notificiation of MITOEAGLE scholarships: 2017-06-10.'''<br />
<br />
<br />
<br />
<br />
[[File:Questions.jpg|left|60px]] <br />
::::» For further questions, please contact the '''MITOEAGLE representatives of the training schools''': <br />
:::::: [[Schlattner U |Uwe Schlattner]]<br />
:::::: [[Engin AB |Ayse Basak Engin]]<br />
::::» Further details on mentorship: consult [[MITOEAGLE Early Career Investigators]]<br />
::::» [[MITOEAGLE_network#Members_in_the_MITOEAGLE_network |List of MITOEAGLE mentors]]<br />
<br />
== Registration ==<br />
<br />
<br />
'''MiP''school'' registration Early Career Investigators and students'''<br />
::::» [[Media:Registration form Training School MITOEAGLE student.pdf| ECI and students registration]]<br />
<br />
::::» [[Media:Registration form Training School MITOEAGLE.pdf|Regular registration ]]<br />
<br />
<br />
'''MITOEAGLE Workshop WG1-4'''<br />
::::» [[Media:Registration_form_MITOEAGLE_Workshop_WG1-4.pdf|Registration form]]<br />
<br />
<br />
== Travel info ==<br />
:::: How to get there? Find out more: [[MiPschool_Obergurgl_2017_Travel info|Travel info]]<br />
<br />
<br />
<br />
==Funding ==<br />
<br />
::::* [[COST Action MITOEAGLE |COST Action CA15203 Mitochondrial fitness mapping - MITOEAGLE]]<br />
<br />
[[Image:Tirol Logo Standortagentur.jpg|thumb|The project MitoFit is funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.|left| 100px]]<br />
[[Image:Medizinische-Uni-Innsbruck-Logo.gif|100px| Medical University Innsbruck]]<br />
[[Image:MitoFit.jpg|80px|link=http://www.mitofit.org/index.php/K-Regio MitoFit|K-Regio MitoFit]]<br />
<br />
<br />
<br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
<br />
<br />
{{MITOEAGLE banner}}<br />
{{Labeling<br />
|additional=2017, ORO, MitoFit, MITOEAGLE, Next<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=File:Logo_Univ_Innsbruck.jpg&diff=135228File:Logo Univ Innsbruck.jpg2017-05-05T12:26:28Z<p>Bitschnau Barbara: Bitschnau Barbara moved page File:Logo Universität Innsbruck.jpg to File:Logo Univ Innsbruck.jpg without leaving a redirect</p>
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<div></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=File:Logo_age_reg.jpg&diff=135227File:Logo age reg.jpg2017-05-05T12:20:23Z<p>Bitschnau Barbara: Bitschnau Barbara moved page File:Logo arge reg.jpg to File:Logo age reg.jpg without leaving a redirect</p>
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<div></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=File:Logo_age_reg.jpg&diff=135226File:Logo age reg.jpg2017-05-05T12:16:59Z<p>Bitschnau Barbara: </p>
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<div></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=File:Logo_Univ_Innsbruck.jpg&diff=135225File:Logo Univ Innsbruck.jpg2017-05-05T12:16:42Z<p>Bitschnau Barbara: </p>
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<div></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=EMBO_2017_Xian_CH&diff=135205EMBO 2017 Xian CH2017-05-05T06:51:58Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{Publication<br />
|title='''Xi'an China''', 2017 Jul 02-05. EMBO Workshop - Mitochondrial quality control, [http://meetings.embo.org/event/17-mito-quality EMBO Workshop 2017]<br />
|info=[http://meetings.embo.org/event/17-mito-quality EMBO Workshop 2017]<br />
|authors=EMBO Workshop<br />
|year=2017-07-02<br />
|journal=MitoGlobal<br />
|abstract= EMBO Workshop - Mitochondrial quality control, [http://meetings.embo.org/event/17-mito-quality EMBO Workshop 2017], Xi'an, CH.<br />
}}<br />
<br />
[[File:EMBO 2017 China.jpg|thumb|270px|right|[http://www.bioblast.at/images/f/ff/EMBO_2017_China.pdf Download Poster EMBO 2017]]]<br />
== Venue ==<br />
::::Nanyang Hotel: 1# XingQing South Rd, 710048 Xi’an, China<br />
::::[http://www.jdnyhotel.com/introduce.php?locale=en-us NanYang Hotel]<br />
==Registration==<br />
<br />
::::*Registration Deadline<br />
::::: We have extended the deadline for registration until 12th of June 2017. <br />
::::*Abstract Submission Deadline<br />
:::::12 June 2017<br />
::::*Payment Deadline<br />
:::::15 June 2017<br />
<br />
<br />
== Programme ==<br />
::::For programme information and also other information like registration fee, venue, speakers, and more details about the Workshop, please view [http://meetings.embo.org/event/17-mito-quality here]<br />
<br />
== Organizer ==<br />
::::[[Chen Q|Prof., Dr. Chen Quan]]<br />
<br />
<br />
{{Labeling<br />
|instruments=<br />
|additional=2017, MitoGlobal<br />
}}<br />
<br />
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Residual_oxygen_consumption&diff=135201Residual oxygen consumption2017-05-05T06:18:05Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=ROX<br />
|description=[[File:ROX.jpg]] '''Residual oxygen consumption''', ROX, is respiration due to oxidative side reactions remaining after inhibition of the [[electron transfer system]] (ETS) in mitochondrial preparations or cells, or in mt-preparations incubated without addition of fuel substrates (in the presence of ADP following a stimulation of the consumption of endogenous fuel substrates: [[State 2]]). Different conditions designated as ROX states (different combinations of inhibitors of CI, CII, CIII and CIV; or respiration of mt-preparations without addition of fuel substrates) may result in consistent or significantly different levels of oxygen consumption. Hence the best quantitative estimate of ROX has to be carefully evaluated. Mitochondrial respiration is frequently corrected for ROX as the [[baseline state]]. Then total [[ROUTINE]], [[LEAK]], [[OXPHOS]] or [[ETS]] (''R'', ''L'', ''P'' and ''E'') respiration is distinguished from the corresponding ROX-corrected, mitochondrial (ETS-linked) fluxes: ''R''(mt), ''L''(mt), ''P''(mt) and ''E''(mt). When expressing ROX as a fraction of ETS capacity ([[flux control ratio]]), total flux, ''E'' (not corrected for ROX), should be taken as the reference. ROX may be related to, but is of course different from [[ROS]] production.<br />
» [[#ROX or non-mitochondrial respiration and potential artefacts |'''MiPNet article''']]<br />
|info=[[Pathway control state]], [[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]]<br />
}}<br />
{{MitoPedia concepts<br />
|mitopedia concept=Respiratory state, Recommended<br />
}}<br />
{{MitoPedia methods}}<br />
{{MitoPedia O2k and high-resolution respirometry}}<br />
{{MitoPedia topics<br />
|mitopedia topic=EAGLE<br />
}}<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[Antimycin A]]<br />
::::» [[Baseline state]]<br />
::::» [[CI]]<br />
::::» [[CII]] <br />
::::» [[Complex III|CIII]]<br />
::::» [[Electron transfer system]]<br />
::::» [[ETS]]<br />
::::» [[Flux control ratio]]<br />
::::» [[Instrumental background oxygen flux]]<br />
::::» [[LEAK]]<br />
::::» [[Malonate]] <br />
::::» [[Mitochondrial preparations]] <br />
::::» [[OXPHOS]] <br />
::::» [[Permeabilized tissue or cells]]<br />
::::» [[Rotenone]]<br />
::::» [[ROUTINE respiration]]<br />
::::» [[State 2]]<br />
::::» [[SUIT protocol]]s<br />
</div><br />
</div><br />
<br /><br />
<br />
__TOC__<br />
= Residual oxygen consumption: a baseline correction of mitochondrial respiration =<br />
{{Publication<br />
|title=Gnaiger E (2016) Residual oxygen consumption: a baseline correction of mitochondrial respiration. Mitochondr Physiol Network 2016-01-28, updated 2016-11-19.<br />
|info=<br />
|authors=OROBOROS<br />
|year=2016<br />
|journal=MiPNet<br />
|abstract=[[File:ROX.jpg]] '''[[Residual oxygen consumption]]''' (ROX) is sometimes referred to as 'non-mitochondrial respiration'. This may be correct to a large extent, but is not entirely accurate. In a preparation of purified isolated mitochondria, a small but significant ROX is observed after appropriate correction for [[instrumental background oxygen flux]]. In this case, ROX is 'mitochondrial non-ETS' rather than ‘non-mitochondrial’ respiration. In permeabilized and intact cells, ROX may be higher than in isolated mitochondria, and this increased part then would be a measurement of non-mitochondrial respiration. Keilin (1926) introduced and accurately defined the term residual respiration.<br />
|mipnetlab=AT Innsbruck Gnaiger E<br />
}}<br />
{{Labeling<br />
|area=Respiration<br />
|pathways=ROX<br />
|instruments=Theory<br />
}}<br />
<br />
== Abbreviations ==<br />
<br />
::::* '''Previous editions''' (including [[Gnaiger 2014 MitoPathways]]): '''ROX-corrected''' [[ROUTINE]], [[LEAK]], [[OXPHOS]] or [[ETS]] (''R'', ''L'', ''P'' and ''E'') is distinguished from the corresponding '''apparent''' fluxes that are not corrected for ROX. ''Apparent'' fluxes (not corrected for ROX; ''R''´, ''L''´, ''P''´ and ''E''´) places the terminology into the conceptual context of physical chemistry (including kinetics), where e.g. apparent equilibrium constants, ''K''<sub>eq</sub>´ (apparent Michaelis-Menten kinetic constants, ''K''<sub>m</sub>´) are carefully distinguished from the less accessible (or inaccessible) 'true' constants, ''K''<sub>eq</sub> (''K''<sub>m</sub>).<br />
<br />
::::* '''Mitochondrial physiology nomenclature''': Despite the fact that the previously used system of abbreviations is linked to a conceptually consistent framework of physical chemistry, generalized applicability may gain from a shift to a pragmatic emphasis of the experimental context of mitochondrial physiology. ROX correction is a baseline correction, which may be evaluated by a direct measurement within an experimental assay, or by independent test experiments for critical evaluation of the best estimate for a particular ROX correction. The ROX correction, therefore, may be emphasized by labelling the ROX-corrected values as mitochondrial (mt) fluxes: ''R''(mt), ''L''(mt), ''P''(mt) and ''E''(mt). Unfortunately, the total ''R'', ''L'', ''P'' and ''E'' values in this mitochondrial physiology system carry opposite meanings to the physical chemistry system of terminology. Confusion may be avoided in this context by explicit abbreviations, ''R''(total), ''L''(total), ''P''(total) and ''E''(total). Such lengthy abbreviations are not really practical.<br />
<br />
::::* '''Agreement''' is required for paving the way towards consistent data reporting and managing a meaningful database (but agreement is not even reached in hot debates on caloric units [J versus cal] or frozen debates on commonly used temperature scales [°C versus Faraday]. Unfortunately, the two systems of abbreviations for ROX correction are not compatible. It is of practical importance to use short and well defined symbols. As a practical guideline, therefore, the simplest solution is to define in a given context, if data (and symbols used) refer to total or ROX-corrected results, and then use the simple symbols ''R'', ''L'', ''P'' and ''E'' in such a defined context.<br />
<br />
<br />
== Keilin 1929: Residual respiration ==<br />
:::: 'KCN, H<sub>2</sub>S and CO combine with some of the components of oxidase forming an inactive compound, with the result that cytochrome, or at least its components ''a''’ and ''c''’, as well as paraphenylenediamine added to the cells, are not oxidised. The respiratory process can be still carried out through the medium of some autoxidisable carriers such as haemochromogens, haematins, the component b’ of cytochrome, or some as yet unknown autoxidisable substances. This '''residual respiration''', according to the nature of the cell, may represent a larger or smaller fraction of the total respiration of the cell.'<br />
:::: Keilin D (1929) Cytochrome and respiratory enzymes. [[Keilin_1929_Proc R Soc London Ser B |Proc R Soc London Ser B 104:206-52.]]<br />
<br />
<br />
== Experimental tests on ROX ==<br />
<br />
=== Is the preparation fully permeabilized? ===<br />
:::: ROX may be estimated in [[mitochondrial preparations]] in [[SUIT protocol]]s which start at a respiratory state without added CHO substrates and without inhibitors, such that continuation of the SUIT protocol is possible for OXPHOS analysis. Some critical questions arise for evaluation, if this respiratory 'ROX state' represents a valid condition for estimation of ROX, and for comparison with ROX measured at the end of a SUIT protocol after titration of substrates and inhibitors of key elements of the electron transfer system (e.g. [[Rotenone]], [[Malonate]] and [[Antimycin A]] as inhibitors of [[CI]], [[CII]] and [[Complex III|CIII]]). For evaluation of such an initial estimate of ROX, the following considerations are suggested.<br />
<br />
::::# A preparation of [[permeabilized tissue or cells]], P<sub>tic</sub>, may contain a fraction of intact, non-permeabilized cells. Non-permeabilized cells have [[ROUTINE respiration]] that<br />
::::## is higher than ROX;<br />
::::## is not stimulated by addition of ADP;<br />
::::## is not diminished over prolonged periods of time due to the presence of cellular endogenous substrates;<br />
::::## can be stimulated by uncoupler titration;<br />
::::## can be inhibited by Rot (CI) and Ama (CII), etc.<br />
::::# Respiration of permeabilized tissue or cells incubated in a medium with significant concentrations of mitochondrial substrates<br />
::::## is higher than ROX;<br />
::::## is stimulated by addition of ADP (compare 1.2);<br />
::::## is not diminished over prolonged periods of time due to the presence of exogenous substrates in the medium;<br />
::::## can be stimulated by uncoupler titration,<br />
::::## can be inhibited by Rot (CI) and Ama (CII), etc.<br />
::::# In contrast, the acutal level of ROX of fully permeabilized cells responds to such tests as<br />
::::## ROX is obtained after gradual depletion of mitochondrial internal substrates (gradual decline of oxygen consumption);<br />
::::## ADP or uncoupler titrations accelerate this decline;<br />
::::## Rot and Ama do not inhibit ROX; <br />
::::## uncoupler titration does not stimulate ROX.<br />
<br />
<br />
== Related terms in Bioblast ==<br />
[[Image:OXPHOS-coupled energy cycles.jpg|right|300px||link=Gnaiger 2014 MitoPathways|OXPHOS-coupled energy cycles. Source: The blue book]]<br />
:::: [[File:P.jpg |link=OXPHOS capacity]] [[OXPHOS capacity |OXPHOS]], ''P''<br />
<br />
:::: [[File:R.jpg |link=ROUTINE respiration]] [[ROUTINE respiration |ROUTINE]], ''R''<br />
<br />
:::: [[File:E.jpg |link=ETS capacity]] [[ETS capacity |ETS]], ''E''<br />
<br />
:::: [[File:L.jpg |link=LEAK respiration]] [[LEAK respiration |LEAK]], ''L''<br />
<br />
:::: [[File:ROX.jpg |link=Residual oxygen consumption]] [[Residual oxygen consumption |ROX]], ''R''<br />
<br />
<br />
<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
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<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» List of publications: Residual oxygen consumption'''</span><br />
<div class="mw-collapsible-content"><br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Pathways::ROX]]<br />
|?Was published in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway<br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=descending<br />
}}<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
== Abstracts: Residual oxygen consumption ==<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Pathways::ROX]]<br />
|?Was submitted in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway <br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was submitted in year<br />
|order=descending<br />
}}<br />
</div><br />
</div><br />
<br /></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Permeabilized_muscle_fibers&diff=135160Permeabilized muscle fibers2017-05-04T11:34:16Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=pfi<br />
|description='''Permeabilized muscle fibres''' (pfi) are used as a mitochondrial preparation in respirometry to access mitochondrial function comparable to [[isolated mitochondria]] (imt). pfi are obtained by selectively permeabilizing the plasma membrane mechanically and chemically ([[saponin]]), for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the [[mitochondrial|mt]]-membranes.<br />
<br />
:» MitoPedia topic: [[Mitochondrial preparations]]<br />
|info=[[Pesta 2012 Methods Mol Biol]]<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=Sample preparation<br />
}}<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[Digitonin]] <br />
::::» [[Talk:Permeabilized muscle fibres|Discussion:Permeabilized muscle fibres]]<br />
::::» [[Dyscoupling]] <br />
::::» [[ETS]]<br />
::::» [[HRR-Dissection Set]]<br />
::::» [[MiPNet14.06 Instrumental O2 background|Instrumental O<sub>2</sub> background test]]<br />
::::» [[Isolated mitochondria]]<br />
::::» [[L/E]]<br />
::::» [[Microbalance-Set]]<br />
::::» [[Mitochondrial|mt-membranes]]<br />
::::» [[Mitochondrial_membrane_potential#Mitochondrial_membrane_potential_of_permeabilized_fibres|Mitochondrial membrane potential of permeabilized fibres]]tion <br />
::::» [[Nagarse]]<br />
::::» [[O2k-Fluo_LED2-Module]]<br />
::::» [[MiPNet17.06 IOC66|O2k-Fluorometry Workshop IOC66]]<br />
::::» [[OROBOROS O2k-Catalogue]]<br />
::::» [[OXPHOS]] <br />
::::» [[PBI-Shredder HRR-Set|PBI-Shredder]] <br />
::::» [[P/E]]<br />
::::» [[Saponin]]<br />
::::» [[SUIT protocol]]s<br />
</div><br />
</div><br />
<br /><br />
__TOC__<br />
== High oxygen in permeabilized fibres versus oxygen limitation under hypoxia ==<br />
<br />
:::: Contributed by [[Gnaiger E]] 2012-05-26, edited 2016-08-17. <br />
<br />
=== Respiratory capacity and substrate concentrations ===<br />
:::: When measuring respiration in permeabilized fibres (pfi), the arguments for the appropriate experimental oxygen regime are quite simple: If you are interested in measuring '''mitochondrial respiratory capacity''' ([[OXPHOS]], [[ETS]]), substrate concentrations must be evaluated such that a limitation of respiratory flux by non-saturating substrate concentration can be excluded. Kinetic control studies, therefore, provide the basis of critically examining the appropriate substrate concentration for carbon substrates, ADP, inorganic phosphate and oxygen. Even if ADP concentrations in muscle tissues never reach 5 mM in vivo, we have to increase the ADP concentration to such high or even higher values in pfi, if the kinetics tells us that more physiological 100 µM do not support maximum respiratory flux. <br />
<br />
:::: The same argument applies for oxygen supply. 200 µM (20 kPa) is far higher than physiological intracellular ''p''<sub>O2</sub> in muscle tissue. Nevertheless, isolated mitochondria are most frequently studied in the range of 10 to 20 kPa, rather than at physiological 0.5 to 3 kPa. This is fine as long as a concentration-independent saturating concentration range is obtained, where high substrate concentrations are neither inhibiting nor damaging. Air-level oxygen pressure, however, is effectively hyperoxic in isolated mitochondria ([[Gnaiger 2000 Proc Natl Acad Sci USA|Gnaiger et al 2000]]), but may by hypoxic in permeabilized muscle fibres.<br />
<br />
=== Oxygen kinetics of permeabilized fibres ===<br />
:::: Unfortunately, the oxygen dependence of respiration in permeabilized fibres is about 100-fold higher compared to small intact cells and isolated mitochondria (reviewed by [[Scandurra 2010 Adv Exp Med Biol|Scandurra and Gnaiger 2010]]) due to artificial diffusion gradients resulting from the spatial constraints (long diffusion distances) in the fibre preparation ([[Gnaiger 2003 Adv Exp Med Biol|Gnaiger 2003]], [[Pesta 2012 Methods Mol Biol|Pesta and Gnaiger 2012]]). When incubating pfi below air-level oxygen pressures at a physiological temperature of 37 °C, therefore, it must be shown that the preparation is not oxygen limited due to the spatial configuration of fibre bundles and development of a hypoxic core. Control experiments should be shown (rather than neglecting the problem of oxygen limitation) with traces of ''J''<sub>O2</sub> in the O2k over prolonged periods of time, when oxygen drops from air level to the minimum experimental values, which would provide proof that oxygen flux is stable and not progressively limited by the declining oxygen concentration. In pfi from various species and muscle types (including [[Lemieux 2011 Int J Biochem Cell Biol|human]], rat, mouse and fish heart; [[Pesta 2012 Methods Mol Biol|human]], [[Votion 2012 PLoS One|horse]], rat, mouse, and fish skeletal muscle) oxygen limitation has been shown of pfi respiration below air level ''p''<sub>O2</sub>. Undefined hypoxic limitation of respiration of a core population of mitochondria is not a satisfactory condition, hence '''hyperoxic''' incubation presents the best compromise for respiratory studies with pfi. Unfortunately, oxygen limitation of pfi has been ignored by several research groups ([[Kuznetsov 2008 Nat Protoc|Kuznetsov et al 2008]]; compare [[Kuznetsov_1998_BTK|Kuznetsov et al 1998]]; for a discussion see [[Pesta 2012 Methods Mol Biol|Pesta and Gnaiger 2012]]).<br />
<br />
::::* Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, Påhlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:273-6. - [[Kuznetsov_1998_BTK |Bioblast link]]<br />
::::* Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci USA 97:11080-5. - [[Gnaiger 2000 Proc Natl Acad Sci USA|Bioblast link]]<br />
::::* Gnaiger E (2003) Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. Adv Exp Med Biol 543:39-55. - [[Gnaiger 2003 Adv Exp Med Biol |Bioblast link]]<br />
::::* Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. - [[Scandurra 2010 Adv Exp Med Biol|Bioblast link]]<br />
::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. - [[Pesta 2012 Methods Mol Biol|Bioblast link]]<br />
<br />
=== Oxygen dependence of ROS production - are permeabilized fibres a valid model? ===<br />
[[File:Gnaiger 2001 Respir Physiol New Fig10.jpg|255px|right|thumb|link=]]<br />
[[Bioblast_alert#Bioblast_alert_2012_.2803.29:_2012-03-19| '''Bioblast alert 2012(03)''']] / '''[[Talk:Permeabilized muscle fibres]]'''<br />
<br />
:::: When studying mitochondrial ROS production, oxygen levels have to be controlled as a critical factor involved in hypoxic or hyperoxic stress. Considering uncontrolled oxygen gradients in permeabilized fibres, can this mitochondrial preparation provide a valid model for the study of mt-ROS production and ROS dynamics?<br />
<br />
:::: The topic for uncontrolled oxygen concentration gradients in pfi, therefore, is becoming an even more disturbing issue when studying not only oxygen consumption but also ROS production. Should then oxygen levels be reduced to the more physiological range when working with pfi? In pfi, ROS production may be artificially increased by application of high oxygen pressures prevailing around a peripheral mt-subpopulation. On the other hand, ROS may be reduced in a central hypoxic subpopulation, and again increased if reductive stress increases ROS production at some intermediary ''p''<sub>O2</sub> levels along the oxygen gradient. <br />
<br />
:::: We have tested the oxygen dependence of H<sub>2</sub>O<sub>2</sub> production in a preparation of cardiac mitochondria during the [[MiPNet17.06 IOC66|O2k-Fluorometry Workshop IOC66]]. For illustration and discussion, we used a classical simple substrate (succinate&rotenone or the non-physiological condition of succinate only), and showed quantitatively the pronounced oxygen dependence of H<sub>2</sub>O<sub>2</sub> production in the high oxygen range when mitochondrial respiration is fully oxygen-saturated (Boveris 1977). An illustration of the complex interactions of LEAK respiration, membrane potential, hyperoxia and hypoxia is given in the figure (modfied from [[Gnaiger_2001_Respir Physiol|Gnaiger 2001]]; for the figure legend, click on the figure).<br />
::::* Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - [[Gnaiger_2001_Respir Physiol|Bioblast link]]<br />
<br />
<br />
=== Tissue homogenate versus permeabilized fibres ===<br />
<br />
:::: The conclusion at this stage is that '''pfi may not provide an optimum model for studies of ROS production'''. <br />
<br />
:::: On the other hand, isolated mitochondria (imt) require too much tissue for most of our studies on biopsies and on mice. To address these problems, we initiated a project on an alternative approach, using a high-quality mt-preparation with a high mt-yield. To achieve this goal, we tested the [[PBI-Shredder HRR-Set|PBI-Shredder]] for muscle tissue homogenate preparation, not using any proteases (such as [[nagarse]]) nor [[saponin]] or [[digitonin]], with merely 15 seconds of mechanical treatment, and completing the entire preparation within 10 min. We optimized the tissue homogenate (thom) preparation to obtain a 100% yield of functionally intact mitochondria. Respiration of muscle thom is less oxygen dependent compared to pfi, but some extent of hyperoxia remains necessary compared to imt to avoid any oxygen limitation.<br />
<br />
:::: It is possible to obtain a single thom preparation with 2-4 mg of tissue, but also increase the amount of tissue to 50 mg in a single preparation, which then yields a thom that can be partitioned into several O2k-chambers for technical repeats or application of different protocols, and subsamples can be stored away for other tests. These tests convinced us, such that we are now offering the PBI-Shredder world-wide as an ‘Auxiliary HRR-Tool’. <br />
:::: * PBI-Shredder HRR-Set: Preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 17.02. - [[MiPNet17.02 PBI-Shredder manual|Bioblast link]]<br />
<br />
:::: A summary of test experiments (basic respirometry only) and description of the shredder homogenization procedure is available.<br />
::::* Mitochondrial respiration in permeabilized fibres versus homogenate from mouse myocardium. An application study with the PBI-Shredder. MiPNet17.03 ([[Bioblast editorial board|contact]] us for a pdf file). <br />
<br />
:::: A demo experiment on respiration and hydrogen peroxide production of mouse heart homogenate with the [[O2k-Fluo_LED2-Module]] is shown in the workshop programme ([http://www.oroboros.at/?IOC66 download pdf file from IOC66]). <br />
<br />
'''Add to the discussion: [[Talk:Permeabilized muscle fibres]]'''<br />
<br />
<br />
== Historical perspectives ==<br />
<br />
:::: The skinned muscle fibre technique was originally introduced by Endo (1967), derived from the mechanically skinned skeletal muscle fibre preparation, first introduced by Natori (1954). It was further adapted and modified by Veksler et al (1987) for cardiac muscle fibres, applied by Letellier et al (1992) for the first time for functional diagnosis of mitochondrial myopathies, and is now well established particularly for skeletal muscle fibres (Saks et al 1998). <br />
<br />
:::: Then as now, it was of great importance “to have the characters closely resembling those of the intact fibre” (Natori 1954). The idea was: “from a physiological point of view, it is desirable to study the contractile system in a state as close to the living state as possible” (Saida and Nonomura 1978). <br />
<br />
:::: After removal of the tissue form the species, the procedure allots mechanical separation of muscle tissue in a relaxing solution. Thereafter, muscle tissue is incubated in saponin by gentle agitation. [[Saponin]], a chemical permeabilization agent of plant origin, selectively attacks cholesterol rich (plasma) membranes of cells or muscle fibers, leaving intracellular membrane structures (mitochondria and ER) as well as myofilaments and the cytoskeleton intact. Consequently, the plasma membrane barrier integrity is lost and the cytosol and all solutes are washed out and the composition of the intracellular milieu is quickly equilibrated with the incubation medium. The mitochondria (''in situ'') are then able to use various substrates within a given protocol. <br />
<br />
:::: Plasma membrane permeabilization with [[digitonin]] or [[saponin]] yields effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes, making externally added compounds accessible to the mitochondria. ADP kinetics is fundamentally different in pfi and imt (Saks et al 1988). In addition, oxygen kinetics is shifted by a 100-fold in crease of the ''p''<sub>50</sub> in pfi versus imt (Pesta and Gnaiger 2012).<br />
<br />
::: '''References'''<br />
::::# Natori H (1954) The property and contraction process of isolated myofibrils. Jikei Med J 1<br />
::::# Saida K, Nonomura Y (1978) Characteristics of Ca<sup>2+</sup>- and Mg<sup>2+</sup>-induced tension development in chemically skinned smooth muscle fibers. J Gen Physiol 72.<br />
::::# Endo M (1967) Regulation of contraction relaxation of cycle (in japanese). Gen Assoc Jpn Med Congr Proc. 17:193-7.<br />
::::# Veksler VI, Kuznetsov AV, Sharov VG, Kapelko VI, Saks VA (1987) Mitochondrial respiratory parameters in cardiac tissue: A novel method of assessment by using saponin-skinned fibers. Biochim Biophys Acta 892:191-6. - [[Veksler 1987 Biochim Biophys Acta|Bioblast link]]<br />
::::# Letellier T, Malgat M, Coquet M,Moretto B, Parrot-Roulaud F, Mazat JP (1992) Mitochondrial myopathy studies on permeabilized muscle fibers. Pediatr Res 32:17–22. - [[Letellier 1992 Pediatr Res|Bioblast link]]<br />
::::# Saks VA, Veksler VI, Kuznetsov AV, Kay L, Sikk P, Tiivel T, Tranqui L, Olivares J, Winkler K, Wiedemann F, Kunz WS (1998) Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol Cell Biochem 184:81-100. - [[Saks 1998 Mol Cell Biochem|Bioblast link]]<br />
::::# Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. - [[Pesta 2012 Methods Mol Biol|Bioblast link]]<br />
<br />
<br />
== Experimental SOP ==<br />
<br />
=== Addition of fibres to the O2k chamber ===<br />
<br />
::::# While preparing the permeabilized fibres, perform oxygen calibration and an [[MiPNet14.06 Instrumental O2 background|instrumental O<sub>2</sub> background test]].<br />
::::# The final state of the O2k chambers before addition of the fibres should be 450 µM O<sub>2</sub> in the closed chamber.<br />
::::# Immediately after wet weight determination, quickly open the O2k chamber, instert the fibre and close, obtaining an initial O<sub>2</sub> concentration of c. 400 µM.<br />
::::# Restart DatLab to define time zero as the time when fibres have been added. <br />
::::# Immediately add P or G or Oct or Pal or .., then add M. Do not leave fibres without substrate. Do not add M, which may lead to oxaloacetate accumulation, which may prolong establishment of a steady-state after addition of P or G or Oct or Pal. A counter-argument might be (as a basis of our published protocols): In the initial phase without added substrates, endogenous substrates are oxidized preferentially when compared to a state with immediate addition of substrates. The prolonged LEAK state (rather than initial substrate-depleted state) proposed here in the alternative initial phase of the experiment will provide a prolonged evaluation of stabilization of flux (not disturbed by substrate additions), but stress due to substrate limitation may be worse than stress due to high membrane potential. <br />
<br />
<br />
=== Chemicals and media ===<br />
<br />
::: '''''What is the optimum ADP concentration to stimulate maximum muscle fibre respiration?'''''<br />
<br />
:::: Especially in muscle fibers an ADP concentration of 5 mM and higher needs to be applied to be in the saturating range, an ADP titration might be required to evaluate, whether the applied concentration is saturating.<br />
<br />
:::: [[Pesta 2012 Methods Mol Biol]]: At a high apparent ''K''<sub>m</sub> for ADP of 0.5 mM, flux at 2.5 and 5 mM ADP is ADP-limited by 13% and 7% (assuming ''L/P''=0.2). 2.5 mM ADP is saturating in many cases, yet a further increase of ADP concentration provides a test for saturating [ADP]. This is particularly important for evaluation of OXPHOS versus ETS capacity (''P'' versus ''E'' or ''P/E'' coupling control ratio).<br />
<br />
This section needs to be extended. <br />
<br />
:::: [[Mitochondrial_membrane_potential#Mitochondrial_membrane_potential_of_permeabilized_fibres|Mitochondrial membrane potential of permeabilized fibres]]<br />
<br />
<br />
=== Permeabilized fibres: auxiliary HRR-tools ===<br />
<br />
:::: '''Auxiliary HRR-tools''' are offered for fibre preparation ([[HRR-Dissection Set]]) and weight determination [[Microbalance-Set]]. A Peltier-controlled cooling plate will be added to the [[OROBOROS O2k-Catalogue]].<br />
<br />
<br />
=== Tissue storage ===<br />
<br />
:::: Small tissue samples (10 mg wet weight) should be put immediately into a pre-cooled storage medium, e.g. [[BIOPS]], and stored on ice (0 to 4 °C). Isolated mitochondria should be stored at high concentration (50 mg mt-protein/ml) on ice in mitochondrial preservation medium.<ref name ="Gnaiger 2000 Life in the Cold">Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - [[Gnaiger 2000 Life in the Cold|Bioblast link]]</ref> <br />
<br />
:::: Up to 12 hours of cold storage does normally not affect mitochondrial respiratory function of liver (pig: 24 h)<ref name ="Kuznetsov 2002 Analyt Biochem">Kuznetsov AV, Strobl D, Ruttmann E, Königsrainer A, Margreiter R, Gnaiger E (2002) Evaluation of mitochondrial respiratory function in small biopsies of liver. Analyt Biochem 305:186-94. - [[Kuznetsov_2002_Analyt_Biochem|Bioblast link]]</ref> and human muscle biopsies (skeletal muscle: 24 h<ref name ="Skladal 1994 BTK">Skladal D, Sperl W, Schranzhofer R, Krismer M, Gnaiger E, Margreiter R, Gellerich FN (1994) Preservation of mitochondrial functions in human skeletal muscle during storage in high energy preservation solution (HEPS). In: What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3. Innsbruck Univ Press:268-71 - [[Skladal 1994 BTK|Bioblast link]]</ref>; cardiac muscle: 8-12 h<ref name ="Lemieux 2011 Int J Biochem Cell Biol">Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38. - [[Lemieux 2011 Int J Biochem Cell Biol|Bioblast link]]</ref>). <br />
<br />
:::: Muscle biopsies of horses can be stored for several days without loss of function<ref name ="Votion 2012 PLoS One">Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. - [[Votion 2012 PLoS One|Bioblast link]]</ref>).<br />
<br />
:::: Storage time without loss of function is, therefore, tissue and species dependent and should be evaluated experimentally. When a larger tissue sample is available, separate the sample into small (10 mg) pieces, and apply respirometric [[SUIT protocol]]s on subsamples in a time course. In particular, evaluate [[dyscoupling]] (''[[L/E]]'' and ''[[P/E]]'' coupling control ratios), cytochrome ''c'' release,<ref name ="Kuznetsov 2004 Am J Physiol Heart Circ Physiol">Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome ''c'' release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286:H1633–41. - [[Kuznetsov 2004 Am J Physiol Heart Circ Physiol|Bioblast link]]</ref> and [[OXPHOS]] capacities with various substrate combinations.<br />
<br />
== References ==<br />
<br />
<references/><br />
<br />
::::* [[O2k-Publications:_Permeabilized_tissue]]<br />
<br />
:::: The following references describe in detail the basis for working with muscle fibers in the O2k.<br />
<br />
::::# Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. - [[Pesta 2012 Methods Mol Biol|Bioblast link]]<br />
::::# Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38. - [[Lemieux 2011 Int J Biochem Cell Biol|Bioblast link]]<br />
::::# Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. - [[Votion 2012 PLoS One|Bioblast link]]<br />
::::* Preparation of permeabilized muscle fibres for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 14.14. - [[MiPNet14.14 PermeabilizedFibrePreparation|Bioblast link]]<br />
::::* Isolated mitochondria or permeabilized tissues and cells. Mitochondr Physiol Network 11.05. - [[MiPNet11.05 Mitos-PermeabilizedCells|Bioblast link]]<br />
::::* Mitochondrial respiration medium - MiR06. Mitochondr Physiol Network 14.13. - [[MiPNet14.13 Medium-MiR06|Bioblast link]]<br />
::::* MitoPedia topic: [[Permeabilized tissue or cells]].</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Residual_oxygen_consumption&diff=135156Residual oxygen consumption2017-05-04T09:58:05Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=ROX<br />
|description=[[File:ROX.jpg]] '''Residual oxygen consumption''', ROX, is respiration due to oxidative side reactions remaining after inhibition of the [[electron transfer system]] (ETS) in mitochondrial preparations or cells, or in mt-preparations incubated without addition of fuel substrates (in the presence of ADP following a stimulation of the consumption of endogenous fuel substrates: [[State 2]]). Different conditions designated as ROX states (different combinations of inhibitors of CI, CII, CIII and CIV; or respiration of mt-preparations without addition of fuel substrates) may result in consistent or significantly different levels of oxygen consumption. Hence the best quantitative estimate of ROX has to be carefully evaluated. Mitochondrial respiration is frequently corrected for ROX as the [[baseline state]]. Then total [[ROUTINE]], [[LEAK]], [[OXPHOS]] or [[ETS]] (''R'', ''L'', ''P'' and ''E'') respiration is distinguished from the corresponding ROX-corrected, mitochondrial (ETS-linked) fluxes: ''R''(mt), ''L''(mt), ''P''(mt) and ''E''(mt). When expressing ROX as a fraction of ETS capacity ([[flux control ratio]]), total flux, ''E'' (not corrected for ROX), should be taken as the reference. ROX may be related to, but is of course different from [[ROS]] production.<br />
» [[#ROX or non-mitochondrial respiration and potential artefacts |'''MiPNet article''']]<br />
|info=[[Pathway control state]], [[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]]<br />
}}<br />
{{MitoPedia concepts<br />
|mitopedia concept=Respiratory state, Recommended<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=EAGLE<br />
}}<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[Ama]]<br />
::::» [[Baseline state]]<br />
::::» [[CI]]<br />
::::» [[CII]] <br />
::::» [[CIII]]<br />
::::» [[Electron transfer system]]<br />
::::» [[ETS]]<br />
::::» [[Flux control ratio]]<br />
::::» [[Instrumental background oxygen flux]]<br />
::::» [[LEAK]]<br />
::::» [[Mna]] <br />
::::» [[Mitochondrial preparations]] <br />
::::» [[OXPHOS]] <br />
::::» [[Permeabilized tissue or cells]]<br />
::::» [[Rot]]<br />
::::» [[ROUTINE respiration]]<br />
::::» [[State 2]]<br />
::::» [[SUIT protocol]]s<br />
</div><br />
</div><br />
<br /><br />
<br />
__TOC__<br />
= Residual oxygen consumption: a baseline correction of mitochondrial respiration =<br />
{{Publication<br />
|title=Gnaiger E (2016) Residual oxygen consumption: a baseline correction of mitochondrial respiration. Mitochondr Physiol Network 2016-01-28, updated 2016-11-19.<br />
|info=<br />
|authors=OROBOROS<br />
|year=2016<br />
|journal=MiPNet<br />
|abstract=[[File:ROX.jpg]] '''[[Residual oxygen consumption]]''' (ROX) is sometimes referred to as 'non-mitochondrial respiration'. This may be correct to a large extent, but is not entirely accurate. In a preparation of purified isolated mitochondria, a small but significant ROX is observed after appropriate correction for [[instrumental background oxygen flux]]. In this case, ROX is 'mitochondrial non-ETS' rather than ‘non-mitochondrial’ respiration. In permeabilized and intact cells, ROX may be higher than in isolated mitochondria, and this increased part then would be a measurement of non-mitochondrial respiration. Keilin (1926) introduced and accurately defined the term residual respiration.<br />
|mipnetlab=AT Innsbruck Gnaiger E<br />
}}<br />
{{Labeling<br />
|area=Respiration<br />
|pathways=ROX<br />
|instruments=Theory<br />
}}<br />
<br />
== Abbreviations ==<br />
<br />
::::* '''Previous editions''' (including [[Gnaiger 2014 MitoPathways]]): '''ROX-corrected''' [[ROUTINE]], [[LEAK]], [[OXPHOS]] or [[ETS]] (''R'', ''L'', ''P'' and ''E'') is distinguished from the corresponding '''apparent''' fluxes that are not corrected for ROX. ''Apparent'' fluxes (not corrected for ROX; ''R''´, ''L''´, ''P''´ and ''E''´) places the terminology into the conceptual context of physical chemistry (including kinetics), where e.g. apparent equilibrium constants, ''K''<sub>eq</sub>´ (apparent Michaelis-Menten kinetic constants, ''K''<sub>m</sub>´) are carefully distinguished from the less accessible (or inaccessible) 'true' constants, ''K''<sub>eq</sub> (''K''<sub>m</sub>).<br />
<br />
::::* '''Mitochondrial physiology nomenclature''': Despite the fact that the previously used system of abbreviations is linked to a conceptually consistent framework of physical chemistry, generalized applicability may gain from a shift to a pragmatic emphasis of the experimental context of mitochondrial physiology. ROX correction is a baseline correction, which may be evaluated by a direct measurement within an experimental assay, or by independent test experiments for critical evaluation of the best estimate for a particular ROX correction. The ROX correction, therefore, may be emphasized by labelling the ROX-corrected values as mitochondrial (mt) fluxes: ''R''(mt), ''L''(mt), ''P''(mt) and ''E''(mt). Unfortunately, the total ''R'', ''L'', ''P'' and ''E'' values in this mitochondrial physiology system carry opposite meanings to the physical chemistry system of terminology. Confusion may be avoided in this context by explicit abbreviations, ''R''(total), ''L''(total), ''P''(total) and ''E''(total). Such lengthy abbreviations are not really practical.<br />
<br />
::::* '''Agreement''' is required for paving the way towards consistent data reporting and managing a meaningful database (but agreement is not even reached in hot debates on caloric units [J versus cal] or frozen debates on commonly used temperature scales [°C versus Faraday]. Unfortunately, the two systems of abbreviations for ROX correction are not compatible. It is of practical importance to use short and well defined symbols. As a practical guideline, therefore, the simplest solution is to define in a given context, if data (and symbols used) refer to total or ROX-corrected results, and then use the simple symbols ''R'', ''L'', ''P'' and ''E'' in such a defined context.<br />
<br />
<br />
== Keilin 1929: Residual respiration ==<br />
:::: 'KCN, H<sub>2</sub>S and CO combine with some of the components of oxidase forming an inactive compound, with the result that cytochrome, or at least its components ''a''’ and ''c''’, as well as paraphenylenediamine added to the cells, are not oxidised. The respiratory process can be still carried out through the medium of some autoxidisable carriers such as haemochromogens, haematins, the component b’ of cytochrome, or some as yet unknown autoxidisable substances. This '''residual respiration''', according to the nature of the cell, may represent a larger or smaller fraction of the total respiration of the cell.'<br />
:::: Keilin D (1929) Cytochrome and respiratory enzymes. [[Keilin_1929_Proc R Soc London Ser B |Proc R Soc London Ser B 104:206-52.]]<br />
<br />
<br />
== Experimental tests on ROX ==<br />
<br />
=== Is the preparation fully permeabilized? ===<br />
:::: ROX may be estimated in [[mitochondrial preparations]] in [[SUIT protocol]]s which start at a respiratory state without added CHO substrates and without inhibitors, such that continuation of the SUIT protocol is possible for OXPHOS analysis. Some critical questions arise for evaluation, if this respiratory 'ROX state' represents a valid condition for estimation of ROX, and for comparison with ROX measured at the end of a SUIT protocol after titration of substrates and inhibitors of key elements of the electron transfer system (e.g. [[Rot]], [[Mna]] and [[Ama]] as inhibitors of [[CI]], [[CII]] and [[CIII]]). For evaluation of such an initial estimate of ROX, the following considerations are suggested.<br />
<br />
::::# A preparation of [[permeabilized tissue or cells]], P<sub>tic</sub>, may contain a fraction of intact, non-permeabilized cells. Non-permeabilized cells have [[ROUTINE respiration]] that<br />
::::## is higher than ROX;<br />
::::## is not stimulated by addition of ADP;<br />
::::## is not diminished over prolonged periods of time due to the presence of cellular endogenous substrates;<br />
::::## can be stimulated by uncoupler titration;<br />
::::## can be inhibited by Rot (CI) and Ama (CII), etc.<br />
::::# Respiration of permeabilized tissue or cells incubated in a medium with significant concentrations of mitochondrial substrates<br />
::::## is higher than ROX;<br />
::::## is stimulated by addition of ADP (compare 1.2);<br />
::::## is not diminished over prolonged periods of time due to the presence of exogenous substrates in the medium;<br />
::::## can be stimulated by uncoupler titration,<br />
::::## can be inhibited by Rot (CI) and Ama (CII), etc.<br />
::::# In contrast, the acutal level of ROX of fully permeabilized cells responds to such tests as<br />
::::## ROX is obtained after gradual depletion of mitochondrial internal substrates (gradual decline of oxygen consumption);<br />
::::## ADP or uncoupler titrations accelerate this decline;<br />
::::## Rot and Ama do not inhibit ROX; <br />
::::## uncoupler titration does not stimulate ROX.<br />
<br />
<br />
== Related terms in Bioblast ==<br />
[[Image:OXPHOS-coupled energy cycles.jpg|right|300px||link=Gnaiger 2014 MitoPathways|OXPHOS-coupled energy cycles. Source: The blue book]]<br />
:::: [[File:P.jpg |link=OXPHOS capacity]] [[OXPHOS capacity |OXPHOS]], ''P''<br />
<br />
:::: [[File:R.jpg |link=ROUTINE respiration]] [[ROUTINE respiration |ROUTINE]], ''R''<br />
<br />
:::: [[File:E.jpg |link=ETS capacity]] [[ETS capacity |ETS]], ''E''<br />
<br />
:::: [[File:L.jpg |link=LEAK respiration]] [[LEAK respiration |LEAK]], ''L''<br />
<br />
:::: [[File:ROX.jpg |link=Residual oxygen consumption]] [[Residual oxygen consumption |ROX]], ''R''<br />
<br />
<br />
<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» List of publications: Residual oxygen consumption'''</span><br />
<div class="mw-collapsible-content"><br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
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{{#ask:[[Category:Publications]] [[Pathways::ROX]]<br />
|?Was published in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway<br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
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}}<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
== Abstracts: Residual oxygen consumption ==<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Pathways::ROX]]<br />
|?Was submitted in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway <br />
|?Preparation=Preparation<br />
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|?Tissue and cell=Tissue;cell<br />
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</div><br />
<br /></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Residual_oxygen_consumption&diff=135155Residual oxygen consumption2017-05-04T09:55:20Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=ROX<br />
|description=[[File:ROX.jpg]] '''Residual oxygen consumption''', ROX, is respiration due to oxidative side reactions remaining after inhibition of the [[electron transfer system]] (ETS) in mitochondrial preparations or cells, or in mt-preparations incubated without addition of fuel substrates (in the presence of ADP following a stimulation of the consumption of endogenous fuel substrates: [[State 2]]). Different conditions designated as ROX states (different combinations of inhibitors of CI, CII, CIII and CIV; or respiration of mt-preparations without addition of fuel substrates) may result in consistent or significantly different levels of oxygen consumption. Hence the best quantitative estimate of ROX has to be carefully evaluated. Mitochondrial respiration is frequently corrected for ROX as the [[baseline state]]. Then total [[ROUTINE]], [[LEAK]], [[OXPHOS]] or [[ETS]] (''R'', ''L'', ''P'' and ''E'') respiration is distinguished from the corresponding ROX-corrected, mitochondrial (ETS-linked) fluxes: ''R''(mt), ''L''(mt), ''P''(mt) and ''E''(mt). When expressing ROX as a fraction of ETS capacity ([[flux control ratio]]), total flux, ''E'' (not corrected for ROX), should be taken as the reference. ROX may be related to, but is of course different from [[ROS]] production.<br />
» [[#ROX or non-mitochondrial respiration and potential artefacts |'''MiPNet article''']]<br />
|info=[[Pathway control state]], [[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]]<br />
}}<br />
{{MitoPedia concepts<br />
|mitopedia concept=Respiratory state, Recommended<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=EAGLE<br />
}}<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[Ama]]<br />
::::» [[Baseline state]]<br />
::::» [[CI]]<br />
::::» [[CII]] <br />
::::» [[CIII]]<br />
::::» [[Electron transfer system]]<br />
::::» [[ETS]]<br />
::::» [[Flux control ratio]]<br />
::::» [[Instrumental background oxygen flux]]<br />
::::» [[LEAK]]<br />
::::» [[Mna]] <br />
::::» [[Mitochondrial preparations]] <br />
::::» [[OXPHOS]] <br />
::::» [[Permeabilized tissue or cells]]<br />
::::» [[Rot]]<br />
::::» [[ROUTINE respiration]]<br />
::::» [[State 2]]<br />
::::» [[SUIT protocol]]s<br />
</div><br />
</div><br />
<br /><br />
<br />
__TOC__<br />
= Residual oxygen consumption: a baseline correction of mitochondrial respiration =<br />
{{Publication<br />
|title=Gnaiger E (2016) Residual oxygen consumption: a baseline correction of mitochondrial respiration. Mitochondr Physiol Network 2016-01-28, updated 2016-11-19.<br />
|info=<br />
|authors=OROBOROS<br />
|year=2016<br />
|journal=MiPNet<br />
|abstract=[[File:ROX.jpg]] '''[[Residual oxygen consumption]]''' (ROX) is sometimes referred to as 'non-mitochondrial respiration'. This may be correct to a large extent, but is not entirely accurate. In a preparation of purified isolated mitochondria, a small but significant ROX is observed after appropriate correction for [[instrumental background oxygen flux]]. In this case, ROX is 'mitochondrial non-ETS' rather than ‘non-mitochondrial’ respiration. In permeabilized and intact cells, ROX may be higher than in isolated mitochondria, and this increased part then would be a measurement of non-mitochondrial respiration. Keilin (1926) introduced and accurately defined the term residual respiration.<br />
|mipnetlab=AT Innsbruck Gnaiger E<br />
}}<br />
{{Labeling<br />
|area=Respiration<br />
|pathways=ROX<br />
|instruments=Theory<br />
}}<br />
<br />
== Abbreviations ==<br />
<br />
::::* '''Previous editions''' (including [[Gnaiger 2014 MitoPathways]]): '''ROX-corrected''' [[ROUTINE]], [[LEAK]], [[OXPHOS]] or [[ETS]] (''R'', ''L'', ''P'' and ''E'') is distinguished from the corresponding '''apparent''' fluxes that are not corrected for ROX. ''Apparent'' fluxes (not corrected for ROX; ''R''´, ''L''´, ''P''´ and ''E''´) places the terminology into the conceptual context of physical chemistry (including kinetics), where e.g. apparent equilibrium constants, ''K''<sub>eq</sub>´ (apparent Michaelis-Menten kinetic constants, ''K''<sub>m</sub>´) are carefully distinguished from the less accessible (or inaccessible) 'true' constants, ''K''<sub>eq</sub> (''K''<sub>m</sub>).<br />
<br />
::::* '''Mitochondrial physiology nomenclature''': Despite the fact that the previously used system of abbreviations is linked to a conceptually consistent framework of physical chemistry, generalized applicability may gain from a shift to a pragmatic emphasis of the experimental context of mitochondrial physiology. ROX correction is a baseline correction, which may be evaluated by a direct measurement within an experimental assay, or by independent test experiments for critical evaluation of the best estimate for a particular ROX correction. The ROX correction, therefore, may be emphasized by labelling the ROX-corrected values as mitochondrial (mt) fluxes: ''R''(mt), ''L''(mt), ''P''(mt) and ''E''(mt). Unfortunately, the total ''R'', ''L'', ''P'' and ''E'' values in this mitochondrial physiology system carry opposite meanings to the physical chemistry system of terminology. Confusion may be avoided in this context by explicit abbreviations, ''R''(total), ''L''(total), ''P''(total) and ''E''(total). Such lengthy abbreviations are not really practical.<br />
<br />
::::* '''Agreement''' is required for paving the way towards consistent data reporting and managing a meaningful database (but agreement is not even reached in hot debates on caloric units [J versus cal] or frozen debates on commonly used temperature scales [°C versus Faraday]. Unfortunately, the two systems of abbreviations for ROX correction are not compatible. It is of practical importance to use short and well defined symbols. As a practical guideline, therefore, the simplest solution is to define in a given context, if data (and symbols used) refer to total or ROX-corrected results, and then use the simple symbols ''R'', ''L'', ''P'' and ''E'' in such a defined context.<br />
<br />
<br />
== Keilin 1929: Residual respiration ==<br />
:::: 'KCN, H<sub>2</sub>S and CO combine with some of the components of oxidase forming an inactive compound, with the result that cytochrome, or at least its components ''a''’ and ''c''’, as well as paraphenylenediamine added to the cells, are not oxidised. The respiratory process can be still carried out through the medium of some autoxidisable carriers such as haemochromogens, haematins, the component b’ of cytochrome, or some as yet unknown autoxidisable substances. This '''residual respiration''', according to the nature of the cell, may represent a larger or smaller fraction of the total respiration of the cell.'<br />
:::: Keilin D (1929) Cytochrome and respiratory enzymes. [[Keilin_1929_Proc R Soc London Ser B |Proc R Soc London Ser B 104:206-52.]]<br />
<br />
<br />
== Experimental tests on ROX ==<br />
<br />
=== Is the preparation fully permeabilized? ===<br />
:::: ROX may be estimated in [[mitochondrial preparations]] in [[SUIT protocol]]s which start at a respiratory state without added CHO substrates and without inhibitors, such that continuation of the SUIT protocol is possible for OXPHOS analysis. Some critical questions arise for evaluation, if this respiratory 'ROX state' represents a valid condition for estimation of ROX, and for comparison with ROX measured at the end of a SUIT protocol after titration of substrates and inhibitors of key elements of the electron transfer system (e.g. [[Rot]], [[Mna]] and [[Ama]] as inhibitors of [[CI]], [[CII]] and [[CIII]]). For evaluation of such an initial estimate of ROX, the following considerations are suggested.<br />
<br />
::::# A preparation of [[permeabilized tissue or cells]], P<sub>tic</sub>, may contain a fraction of intact, non-permeabilized cells. Non-permeabilized cells have [[ROUTINE respiration]] that<br />
::::## is higher than ROX;<br />
::::## is not stimulated by addition of ADP;<br />
::::## is not diminished over prolonged periods of time due to the presence of cellular endogenous substrates;<br />
::::## can be stimulated by uncoupler titration;<br />
::::## can be inhibited by Rot (CI) and Ama (CII), etc.<br />
::::# Respiration of permeabilized tissue or cells incubated in a medium with significant concentrations of mitochondrial substrates<br />
::::## is higher than ROX;<br />
::::## is stimulated by addition of ADP (compare 1.2);<br />
::::## is not diminished over prolonged periods of time due to the presence of exogenous substrates in the medium;<br />
::::## can be stimulated by uncoupler titration,<br />
::::## can be inhibited by Rot (CI) and Ama (CII), etc.<br />
::::# In contrast, the acutal level of ROX of fully permeabilized cells responds to such tests as<br />
::::## ROX is obtained after gradual depletion of mitochondrial internal substrates (gradual decline of oxygen consumption);<br />
::::## ADP or uncoupler titrations accelerate this decline;<br />
::::## Rot and Ama do not inhibit ROX; <br />
::::## uncoupler titration does not stimulate ROX.<br />
<br />
<br />
== Related terms in Bioblast ==<br />
[[Image:OXPHOS-coupled energy cycles.jpg|right|300px||link=Gnaiger 2014 MitoPathways|OXPHOS-coupled energy cycles. Source: The blue book]]<br />
:::: [[File:P.jpg |link=OXPHOS capacity]] [[OXPHOS capacity |OXPHOS]], ''P''<br />
<br />
:::: [[File:R.jpg |link=ROUTINE respiration]] [[ROUTINE respiration |ROUTINE]], ''R''<br />
<br />
:::: [[File:E.jpg |link=ETS capacity]] [[ETS capacity |ETS]], ''E''<br />
<br />
:::: [[File:L.jpg |link=LEAK respiration]] [[LEAK respiration |LEAK]], ''L''<br />
<br />
:::: [[File:ROX.jpg |link=Residual oxygen consumption]] [[Residual oxygen consumption |ROX]], ''R''<br />
<br />
<br />
<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
== Publications: Residual oxygen consumption ==<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Pathways::ROX]]<br />
|?Was published in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway<br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
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}}<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
== Abstracts: Residual oxygen consumption ==<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Pathways::ROX]]<br />
|?Was submitted in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway <br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was submitted in year<br />
|order=descending<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=O2k-pH_ISE-Module&diff=135154O2k-pH ISE-Module2017-05-04T09:43:55Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{Product<br />
|description='''O2k-pH ISE-Module''': two pH electrodes and reference electrodes and accessories, two [[Stopper\black PEEK\conical Shaft\central+2.3+2.6 mm Port|black PEEK stoppers]], supported by the [[O2k-Core]].<br />
|product id=12400-01<br />
|product type=O2k, O2k-Module, MultiSensor, Stopper, Catalogue<br />
|info= [[MiPNet08.16 pH calibration]]<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware<br />
}}<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[DatLab 6]]<br />
::::» [[DatLab 7]] <br />
::::» [[Talk:O2k-pH_ISE-Module#Media_with_low_buffering_capacity|Discussion:O2k-ph: Media_with_low_buffering_capacity ]]<br />
::::» [[O2k-pH_ISE-Module#Performance.2C_trouble_shooting.2C_and_electrode_lifetime|Electrode lifetime]]<br />
::::» [[ESD]]<br />
::::» [[File:PH-Calibration-List.xls]]<br />
::::» [[MiPNet08.16 pH calibration]]<br />
::::» [[MiPNet14.06 Instrumental O2 background]]<br />
::::» [[MiPNet19.18A O2k-Start]]<br />
::::» [[MiPNet19.18C DatLab Guide]]<br />
::::» [[MiPNet19.18D O2k-calibration]]<br />
::::» [[O2k configuration]]<br />
::::» [[O2k control]]<br />
::::» [[O2k-Core]]<br />
::::» [[O2k-Main Unit#O2k-Series]]<br />
::::» [[O2k signals and output#O2k output |O2k output]]<br />
::::» [[O2k-pH_ISE-Module#Measuring_proton_production]]<br />
::::» [[O2k signals and output#Signal of the O2k-Core and add-on modules |O2k signal]]<br />
::::» [[O2k-TPP%2B_ISE-Module]]<br />
::::» [[O2k-pH_ISE-Module#pH-Stat|PH-Stat]]<br />
::::» [[Layout for DatLab graphs|Reference layouts for DatLab graphs]]<br />
::::» [[TIP]] <br />
</div><br />
</div><br />
<br /><br />
== '''O2k-Catalogue: O2k-pH ISE-Module''' ==<br />
{{#ask: [[Category:Products]] [[Product type::O2k-pH ISE-Module]] <br />
| ?description<br />
| ?product id<br />
| ?product image<br />
|sort=product id<br />
|order=ascending<br />
|mainlabel=Title<br />
}}<br />
<br />
__TOC__<br />
<br />
== O2k signal and output ==<br />
:::# [[O2k signals and output#Signal of the O2k-Core and add-on modules |O2k signal]]: The [[O2k-pH ISE-Module]] is operated through the pX channel of the O2k, with electric potential (volt [V]) as the primary and raw signal<br />
:::# [[O2k signals and output#O2k output |O2k output]]: type I and II<br />
<br />
<br />
== pH changes versus glycolytic flux ==<br />
:::: Measurement of extracellular proton production and glycolytic flux are related under specifically conrolled conditions. Such conditions must be carefully evaluated and controlled, may require some modifications of standard protocols and need data analysis beyond reporting changes of pH.<br />
<br />
::::* Very small buffering capacity is required: To accurately measure biologically induced changes in pH, the buffering capacity of the medium has to be small. This may be addressed either by using or preparing media with a buffering capacity that is low but still sufficient to keep the pH in the desired range for a limited period of time. An alternative approach is to use buffers with very low buffering capacity and keep the pH value inside the desired limits by a [[O2k-pH_ISE-Module#pH-Stat|pH-Stat]]. More Details? [[Talk:O2k-pH_ISE-Module#Media_with_low_buffering_capacity|Consult the discussion page]].<br />
::::* Calculation of proton flux from the observed parameters: [[O2k-pH_ISE-Module#Measuring_proton_production]].<br />
<br />
::::* Limited lifetime of pH electrodes: [[O2k-pH_ISE-Module#Performance.2C_trouble_shooting.2C_and_electrode_lifetime|Electrode lifetime]].<br />
<br />
== O2k-Guide ==<br />
=== Introduction and scope ===<br />
:::: The OROBOROS [[O2k-Core]] supports the modular O2k-MultiSensor extension for recording potentiometric (voltage) signals simultaneously with the oxygen signals in both O2k-chambers. A new pH probe system is described here, consisting of separate reference and measuring electrodes. <br />
<br />
::: '''Definition of terms'''<br />
<br />
::::* '''pH/ISE''': The potentiometric channels may be used with different kinds of pH / ISE (ion selective electrode) modules, (i) with the pH / reference electrode module described here, or (ii) with an ISCE – an ion selective combination electrode, combining reference and measuring electrode into one sensor (pH combination electrodes, other ion selective electrodes; see [[O2k-TPP%2B_ISE-Module]]). The O2k-Core not only includes the two potentiometric channels, but supports two additional amperometric (current) channels for the [[O2k-Fluo_LED2-Module]] or amperometric sensors (NO, H<sub>2</sub>O<sub>2</sub>, etc).<br />
<br />
::::* '''pX''': Potentiometric measurements result in a voltage signal which is typically a linear function of the logarithm of the activity (concentration) of the substance of interest (the ''analyte''). A calibrated pH electrode displays the negative decadic logarithm of the H<sup>+</sup> ion activity (potentia hydrogenii) and thus got its name “pH electrode”. By analogy, an ISE may be used to measure pTPP, pCa, etc., hence the general term “pX” is used to denote the signal from such an ISE.<br />
<br />
<br />
=== The pH electrode: shipping, storage and maintenance ===<br />
:::: The pH electrode is shipped with the glass bulb immersed in a vial containing a wet sponge. The pH electrode can be stored in this condition. Carefully unwind the tape and remove the probe from the protective glass tube.<br />
<br />
:::: '''Conditioning:''' Optimum response time will be obtained after the probe has been exposed to two buffer solutions. Place a pH 4 buffer or equivalent in a beaker and a pH7 buffer or equivalent in a second beaker. Hold the pH electrode and reference electrode together and touch the pH 4 buffer surface, allowing 15-20 seconds of equilibration. Rinse the two electrodes with distilled water and then touch the pH 7 buffer surface in the same manner. Repeat several times.<br />
<br />
:::: '''Handling:''' Be careful not to apply pressure against, or to shock, the inner glass capillary tube.<br />
<br />
:::: '''Cleaning:''' When using the electrode in solutions containing protein, the pH electrode and reference electrode should be soaked in enzyme cleaning solution such as Terg-a-zyme (Alconox Inc), or a chromic/sulfuric acid glass cleaning solution after each use for a few minutes to remove the protein from the glass and the reference junction. This will prolong the lifetime of the electrode. <br />
<br />
:::: '''Storage: Always clean the electrode before storing.<br />
<br />
::::* '''Long-term storage (over 2 weeks):''' Place the electrode into its original container in the same condition in which your received it. Usually this means moistening with distilled water the sponge located in the bottom of the protective glass tube.<br />
<br />
::::* '''Short-term storage:''' The electrode can be left in acidic pH buffer solution, e.g. pH 4.<br />
<br />
:::: '''Troubleshooting:''' If possible try to locate the problem either at the measuring pH or at the reference electrode by switching electrodes. If you have only one reference electrode you can switch to a spare glass barrel for diagnostic purposes. The following text assumes that the problem was located on the reference electrode.<br />
<br />
::::* '''Little or no response:''' Inspect the electrode for visible cracks (usually occurring at the tip of the electrode). If any exists, the electrode is defective and must be replaced. The slightest crack in or around the tip of the electrode may cause the electrode to read about the same in all solutions.<br />
<br />
::::* '''Response pegs off-scale:''' (i) Check the pX gain setting. (ii) Visually inspect the electrode for a broken bulb.<br />
<br />
::::* '''Sluggish response:''' If the electrode becomes sluggish in responding to changes in pH, the response time can be improved using the following procedure:<br />
<br />
::::# Clean the electrode as described above.<br />
::::# Soak the electrode in 0.1 N HCl for 5 minutes, followed by soaking in 0.1 N NaOH for 5 minutes. After repeating several times, rinse the electrode thoroughly with distilled water. The electrode can then be calibrated in the usual manner.<br />
<br />
<br />
=== Reference electrode: assembly, storage and maintenance ===<br />
:::: The electrode is composed of an internal silver-silver chloride electrode with an internal filling solution of 3 M KCl saturated with AgCl. Before the electrode can be placed into operation, the glass reference barrel must be filled with the internal reference solution supplied.<br />
<br />
:::: '''Filling the reference barrel:'''<br />
::::# Unscrewing the white plastic cap: Remove the upper part of the cap with the attached silver wire. Pull the glass out of the lower part of the cap.<br />
::::# The internal reference solution is added to the glass tube using the provided electrolyte bottle and polyethylene tubing: Insert filling tube into nipple of electrolyte bottle. Push until tube locks into place. Insert tube into reference barrel and squeeze bottle. Fill reference barrel up to approximately 1.5 cm (approx. 0.5 inch) from top.<br />
::::# After filling the glass barrel with the reference electrolyte, the silver wire is inserted into the glass tube and the electrode cap is re-assembled.<br />
<br />
::::'''Cleaning the electrode: '''To wash the reference electrode between runs, rinsing is recommended in the sequence water, pure ethanol, and water. This procedure should be usually sufficient to prevent carry-over even of hydrophobic inhibitors, since the reference electrode is made of non-hydrophobic materials. Immersion into pure ethanol for longer periods of time should be avoided to prevent blocking of the ceramic diaphragm in an assembled electrode. When using the electrode in solutions containing higher concentrations of protein, the electrode should be soaked in a dedicated enzyme cleaning solution or a chromic/sulfuric acid glass cleaning solution after each use for 10-15 seconds to remove the protein from the glass and the reference junction. This will prolong the useful life of the electrode. <br />
<br />
:::: '''Storing the electrode:''' Always clean the electrode before storing. Protect reference electrodes from light during storage, e.g. by wrapping them in aluminum paper.<br />
<br />
::::* Short term: Place the tip of the electrode in a test tube or beaker containing reference electrolyte (3 M KCl). Falcon type 15 ml vials are well suited. If necessary refill electrolyte before use.<br />
<br />
::::* Long-term (>4 weeks): Remove the glass barrel containing the electrolyte and store the entire glass barrel in a closed test tube filled with the reference electrolyte. Rinse the silver wire and electrode cap to remove the salt solution and dry using an absorbent towel. Store in the accessory box or any closed container to keep dust off of the electrode and protect from light.<br />
<br />
:::: '''Troubleshooting: '''If possible try to locate the problem either at the measuring pH or at the reference electrode by switching electrodes. If you have only one reference electrode you can switch to a spare glass barrel for diagnostic purposes. The following text assumes that the problem was located on the reference electrode.<br />
<br />
::::* '''Little or no response: '''Inspect the electrode for visible cracks. If any exists, the glass barrel is defective and must be replaced with a spare. The slightest crack in or around the tip of the electrode may cause the electrode to read about the same in all solutions.<br />
<br />
::::* '''Response pegs off-scale: '''<br />
::::# Check the pX gain setting.<br />
::::# Visually inspect the electrode for broken or dissolving internal Ag-AgCl wire or for inadequate volume of reference electrolyte. Reference electrolyte level should be above the Ag-AgCl element.<br />
::::# Blocked or clogged liquid junction – clean electrode tip first then soak the tip of the electrode in warm (not hot) distilled water for 5 to 10 minutes. If still clogged, then soak overnight in distilled water or replace reference barrel with spare barrel supplied.<br />
<br />
=== O2k-MultiSensor System ===<br />
:::: Applications of the O2k with one or two potentiometric electrodes (ISE or pH) require an O2k-Core or an instrument with electronic MultiSensor upgrading. In addition to the two polarographic (amperometric) oxygen channels of the basic O2k, the O2k-Core and the MultiSensor system provide two electronic channels for potentiometric (voltage) measurements with ISE or pH (O2k Series B upwards). The Multisensor function of O2k Series D (and higher) is extended further with two additional amperometric channels (current measurement; for NO, etc.). Please see [[O2k-Main Unit#O2k-Series]] for how to determine the series of an oxygraph.<br />
<br />
==== O2k-Core (O2k Series D and higher) ====<br />
:::: '''Connect:''' In O2k Series D (and higher), pH and reference electrodes are directly connected to the plugs on the front side of the O2k. Insert the connector of the pH electrode into the BNC plug labelled “pX” and the connector of the reference electrode to the 2 mm pin plug labelled “Ref”. See [[MiPNet19.18A O2k-Start]].<br />
<br />
:::: '''Gain:''' The gain of the pX channel can be selected in the DatLab software in the O2k configuration window. For measurements with the OROBOROS pH system, a gain of 20 is suggested. Usually it will not be necessary to change the gain for pH work.<br />
<br />
==== O2k Series B and C, pX upgrade (installed before 2011) ====<br />
:::: [[Image:MS_connector.jpg|right|150px|alt= connecting the MS connector to the oxygraph]][[Image:MS_connector_electrodes.jpg|right|150 px|alt= connecting the electrodes to the MS connector]]'''Connect:'''To connect two electrodes (pH + reference electrode) to the MultiSensor BNC plug of the O2k (Series B and C), a MultiSensor Connector has to be installed. The MultiSensor Connector has a black cable with a male BNC plug and a 2 mm plug on its instrument side (facing the O2k), and a female BNC plug and a 2 mm plug on the opposite side for connecting the electrodes. Additionally, an Allen key and a cable with a 2 mm pin and a spade terminal are included in the MultiSensor Connector set.<br />
<br />
:::: First this additional cable should be attached to the O2k housing. Loosen one of the lower screws on the front side, bottom panel of the O2k (using the supplied Allen key), insert the connection of the thin black cable (spade terminal, red) and tighten the screw again. This additional cable provides a grounding connection to the O2k which improves signal stability. The cable can be left attached to the Oxygraph even when the MultiSensor Connector is not in use and not attached to the O2k. <br />
<br />
:::: For measurements connect the black cable of the MultiSensor Connector with the BNC plug to the multi-sensor BNC port of the Oxygraph, marked “pX”. Then plug the 2 mm pin of the cable attached to the Oxygraph housing into the 2 mm plug of the MultiSensor Connector that is situated on the same side as the black cable. To have the BNC cable and the 2 mm plug in correct positions versus the Oxygraph just turn the MultiSensor Connector over, if necessary. The reference electrode is then connected to the front side 2 mm plug and the measuring electrode (pH) to the front side BNC plug of the MultiSensor Connector.<br />
<br />
:::: [[Image:Gain Serie C.jpg|right|150 px|alt= setting the gain for Series A to C at the bottom side of the O2k]]'''Gain: '''The gain and offset of both potentiometric MultiSensor channels can be adjusted by turning the O2k housing on its side. At the bottom of the O2k there are 4 adjustable screws and a label indicating their functions. <br />
<br />
:::: Turning the screws clockwise increases; counter clockwise decreases gain and offset settings. For pH measurements between pH 4 and 10 it should not be necessary to change these settings. However, the settings might have been changed before for work with a different electrode. If you have set up your system and get a raw voltage beyond +9 or -9 volts, decrease the gain by one or two counter clockwise turns of the screw, repeat if necessary. Importantly, the raw voltage displayed in DatLab is already the amplified signal. It is usually not necessary to change the offset setting.<br />
<br />
==== O2k Series B and C, pX upgrade (installed after 2011-01-01) ====<br />
:::: '''Connect:'''For O2k Series B to C with a pX upgrade installed after 2010, pH and reference electrodes are directly connected to the plugs on the front side of the O2k housing, as described above for O2k Series D (and higher). No special MultiSensor Connector is needed for such instruments. Insert the connector of the pH electrode into the BNC plug (nearest to the chamber) and the connector of the reference electrode to the 2 mm pin plug beside the BNC plug ([[MiPNet19.18A O2k-Start]]).<br />
<br />
:::: '''Gain: '''The gain of both potentiometric MultiSensor channels can be adjusted by turning the O2k housing on its side. At the bottom of the O2k there are 2 rotating switches and a label indicating their functions. The gain can be set to 10, 20, 40, or 80. The factory setting is a gain of 20. Usually a gain of 20 will also be very suitable for pH measurements. For some specific, extremely high-resolution pH measurements a higher gain might be advantageous to avoid limitation of resolution by digital noise. It is expected that for most users there never will arise the necessity to change the gain setting.<br />
<br />
:::: If you have set up your system and get a raw voltage beyond +9 or -9 volts, decrease the gain. Importantly, the raw voltage displayed in DatLab is already the amplified signal.<br />
<br />
=== Operation instructions ===<br />
==== Insertion of electrodes: bubble-free filling of the chambers ====<br />
:::: '''MultiSensor vs. Standard stoppers: '''The introduction of several (large) electrodes into the O2k-chamber through the stopper requires the use of a special “MultiSensor stopper”. The standard stopper has a concave shape on its end inserted into the chamber, with a single capillary (gas-escape/titration capillary) in the centre of the stopper (the highest point when inserted). The end of the pH-MultiSensor stopper is angular with one capillary and two electrode inlets. The gas-escape/titration capillary is at the side of the stopper at the highest point when inserted.<br />
<br />
:::: '''Preventing bubbles:''' When inserting the stopper into the O2k-chamber filled with aqueous medium, gas bubbles are guided into the gas-escape/titration capillary and pushed out of the chamber. This is more effective, however, with the standard stopper than the MultiSensor stopper. Therefore, great care should be taken to avoid the trapping of bubbles during initial insertion. The single most important point for prevention of bubble formation is to close the chamber only after full thermal equilibrium has been established. <nowiki>The best criterion for thermal equilibrium is a stable oxygen signal, with a slope near zero in the “open chamber” configuration used for oxygen sensor calibration, see </nowiki>[[MiPNet12.08]].<br />
<br />
::::# Fill the chamber with medium (2.6 ml for a 2 ml chamber) allowing for a well-defined air space when stirred (see [#section42 Section 4.2]).<br />
::::# Place the stoppers on top of the chambers but do not yet close them. Activate stirring. A gas phase similar to the one for air calibration has to be visible. Using Graph layout “1. Calibration Gr3 Temp.”, wait until temperature, Peltier power, and oxygen concentration are stable and the slope of oxygen concentration is near zero (±1 pmol∙s<sup>1</sup>∙ml<sup>1</sup>).<br />
::::# Calibrate the oxygen signal (air calibration), see [[MiPNet19.18D O2k-calibration]].<br />
::::# Stop the stirrers, and insert the stoppers completely into the chambers.<br />
::::# Insert the reference electrode into slightly larger mm) inlet of the stopper (it won’t fit into the more narrow inlet, so it is a good idea always to start with the reference electrode to find to correct inlets). If a gas bubble remains in the chamber (but liquid is on top of the stopper) try to remove the gas bubble: inserting a short needle (if possible with a flat tip) without an attached syringe into the titration port usual removes any bubbles from the inlet, thereby allowing the big bubble to escape from the chamber. Smaller bubbles may be brought nearer to the gas-escape capillary by starting and stopping the stirrer several times. It may be necessary to lift the entire stopper (including pH electrode) to a position above the liquid phase and insert it again.<br />
::::# Make sure that the smaller inlet for the reference electrode is totally filled with liquid – if necessary add more pre-warmed liquid (same composition as in the chamber) to the top of the stopper.<br />
::::# Insert the reference electrode into the chamber. Move it up and down to get rid of any bubbles that might be trapped in its inlet. Switch on the stirrer and check for any bubbles. If there are bubbles, repeat the instructions described above.<br />
::::# Connect the electrodes to their proper plugs, see above. <br />
::::# Aspirate all excess liquid from the top of the stopper, making sure the top is dry and no liquid film connects the different inlets.<br />
<br />
:::: The uncorrected slope of the oxygen concentration should now be in the usual range for a closed chamber at atmospheric saturation (2 - 4 pmol∙s<sup>1</sup>∙ml<sup>1</sup>). Considerably different fluxes may indicate that there is a liquid “bridge” on top of the stopper connecting at least two different inlets, allowing the circulation of liquid between the chamber and the top of the stopper.<br />
<br />
==== Volume calibration with MultiSensor stoppers ====<br />
:::: When using an MultiSensor stopper, the pH and reference electrodes must be in place when calibrating the O2k-chamber volume. This is similar to volume calibration with standard stoppers, see [[MiPNet12.06]].<br />
<br />
::::# Add to the dry O2k-chamber containing the stirrer bar a water volume accounting for the final chamber volume (2 ml) plus the additional dead volume in the capillary and spaces between electrodes and inlets. For the OROBOROS pH Assembly (ion selective electrode + reference electrode), this additional volume is approximately 0.14 ml. Therefore, the necessary volume to calibrate a chamber volume of 2 ml with the OROBOROS pH system is 2.14 ml.<br />
::::# Start stirring, cover the chamber with a Perspex cover or a loosely placed stopper, and wait for equilibration. To avoid creating bubbles during the calibration process it is very important to allow for full thermal equilibration of the liquid in the chamber. Continue with volume calibration only after reaching the conditions for oxygen calibration at air saturation (stable temperature and Peltier power, near-zero uncorrected oxygen flux (±1 pmol∙s<sup>1</sup>∙ml<sup>1</sup>).<br />
::::# Prepare the MultiSensor stopper as described for a standard stopper (loosening the calibration ring, drying the stopper), making sure that the three inlets are dry. Remove the pH and the references electrode from their respective storage solutions. Dry their shafts with a paper towel (do not use a paper towel directly on glas bulb of the pH electrode or the diaphragm of the reference electrode). Insert the electrodes into the MultiSensor stopper.<br />
::::# Stop the stirrer. Place the stopper on top of the chamber with a loosened volume calibration ring slid down to the chamber holder. Insert the MultiSensor stopper slowly into the unstirred chamber carefully observing first the diminishing gas phase in the chamber. Then focus on the top of the stopper. Stop the insertion as soon as the first drop of liquid appears on the top of the stopper. This may be visible first on top of the gas-ejection capillary comparable to the standard stoppers, but it may also occur at the edge of the reference electrode or the pH electrode.<br />
::::# Fix the position of the volume calibration ring by tightening the screw as in the procedure with a standard stopper.<br />
<br />
==== Handling issues during an experiment ====<br />
:::: Two problems have to be avoided while running an experiment with a MultiSensor stopper:<br />
<br />
::::(a) Introduction of bubbles: After the chamber was filled as described, no gas bubbles should be either in the chamber or in the capillary. <br />
<br />
::::(b) Circulation of liquid between the top of the stopper and the internal chamber needs to be prevented by aspirating any excess liquid form the top of the stopper. These conditions have to be maintained during the entire experiment, removing excess liquid from the stopper after any titration. <br />
<br />
<br />
:::: '''Injections: '''Before inserting a syringe needle into the stopper (manual or TIP syringe), make sure that the capillary is filled with liquid – if necessary, place a drop of liquid on top of the capillary - then remove any bubbles from the capillary by using a needle without an attached syringe. A gas-escape/titration capillary filled with liquid without any gas bubbles provides good visibility through the capillary to the light within the chamber. If you cannot see the light, the capillary is blocked by gas bubbles. These need to be removed. Similarly, when the stirrer is switched off, an internally trapped gas bubble might move into a position to block the light, which can be checked further by switching the stirrer on and off.<br />
<br />
:::: Insert the needle and perform the titration (manual or TIP2k). After removing the needle, remember to aspirate any excess liquid from the top of the stopper that has been ejected from the constant-volume chamber during titration. It is important to minimize the time span during which a liquid bridge exists between the different inlets through the stopper.<br />
<br />
==== Instrumental background oxygen flux ====<br />
:::: Instrumental oxygen background parameters are used to correct on-line biological oxygen flux [[MiPNet14.06 Instrumental O2 background|MiPNet14.06]]. Instrumental O<sub>2</sub> background tests have to be carried out with the MultiSensor stopper and all electrodes in place. Instrumental background parameters obtained with standard stoppers cannot be used to calculate biological oxygen consumptions obtained in a MultiSensor experiment.<br />
<br />
<br />
::: '''Dithionite background'''<br />
<br />
:::: Because of difficulties involved in opening and closing the O2k-chamber closed with a MultiSensor stopper and electrodes, it is strongly recommended to use the instrumental background procedure based on dithionite injections, see [[MiPNet14.06 Instrumental O2 background]]. This method avoids repeated opening and closing of the O2k-chamber. To run an instrumental oxygen background experiment, set up the chambers and electrodes as described above and then follow the procedure see [[MiPNet14.06 Instrumental O2 background|MiPNet14.06]].<br />
<br />
::: '''Instrumental background parameters for oxygen flux'''<br />
<br />
:::: An O2k-chamber with a MultiSensor stopper has a higher oxygen back diffusion, ''a''<sup>0</sup> at zero oxygen concentration, as compared with a standard stopper. In a 2 ml chamber using the OROBOROS pH system in MiR06 at 37 °C, with an oxygen regime from air saturation to low oxygen, the backdiffusion parameter, ''a''°, typically ranges from -4 to -8 pmol∙s<sup>1</sup>∙ml<sup>1</sup>. <nowiki>If more negative fluxes (< -10 </nowiki>pmol∙s<sup>1</sup>∙ml<sup>1</sup>) are detected in the background experiment, this is a strong indication that a liquid bridge exist on the top of the stopper. This problem can be solved by simply aspirating any excess liquid from the top of the stopper.<br />
<br />
==== pH calibration ====<br />
:::: [[MiPNet19.18D_O2k-calibration]] and [[MiPNet08.16 pH calibration]].<br />
<br />
==== Performance, trouble shooting, and electrode lifetime ====<br />
:::: '''Performance criteria '''can be assessed by two tests:<br />
<br />
:::: '''1. Calibrations''' must be reproducible: After performing a two point calibration, reinserting the electrodes in the first used calibration buffer should give the correct calibrated pH value. The slope and intercept of the calibration can be copied to a spreadsheet file ([[File:PH-Calibration-List.xls]]) to obtain a track record..<br />
<br />
:::: '''2.Drift:''' The drift in the medium without biological sample hast to be small as compared to pH changes expected from the sample. The drift will be different in different media (buffering capacity), so for each used system the necessary experience has to be gained. The drift is the first time derivative of the pH signal, so in DatLab it will be shown as “pX Slope”. After proper calibration the unit of this plot will be mpH/s. <br />
<br />
:::: '''Trouble shooting: '''If the required performance criteria are not reached, the following steps should be tested:<br />
<br />
::::# Set the polarisation voltage of the O2 sensor to 0 V in the O2k Control window. Observe any effects on the pX raw signal. A tiny potential jump is acceptable. If a drift in the pX signal is either increased or reduced by this test or an extreme jump in the signal observed, the membrane of the polarographic oxygen sensor (POS) should be replaced. Reset the polarisation voltage to 800 mV after the test.<br />
::::# If you have two pH electrodes: Locate the problem to either the reference electrode or the pH electrode by switching either only the pH or only the reference electrode between chambers.<br />
::::# Follow the specific trouble shooting procedures for the reference electrode or the pH electrode described above.<br />
::::# '''pH electrode lifetime: '''All glass pH electrodes do have a limited lifetime due to aging of the glass membrane. After 1.5 to 2 years a loss of performance will occur even without use. Therefore, we strongly recommend to obtain pH electrodes only when required for a defined project and not buy the electrodes ahead of need.<br />
<br />
=== Measuring proton production ===<br />
==== From the change of the pH signal ====<br />
<br />
:::: To calculate the actual proton flow in the system from the observed change in the pH signal the buffering capacity of the medium has to be determination of before the introduction of sample. This is best achieved by setting up the [[TIP]] with HCl in the syringes and simulate the expected proton production of the sample by setting an appropriate flow rate for the TIP. In the simplest implementation just one flow rate is used for about 5 minutes. From the first time derivative of the pH signal the buffering capacity can be calculated. Afterwards the TIP has to be re-fitted with syringes containing KOH (NaOH) for using the pH-Stat during the actual biological experiment. The observed rates of pH changes during the biological experiment (pX slope/ first time derivative of pH signal) can then be directly converted to Proton flow values using the buffering capacity determined before. This is shown in the spreadsheet file "pH Stat Template Buffer Capacity_one_point" In a more sophisticated approach a multiple point calibration is done: Several proton flows are simulated before the experiment, a linear regression between set proton flow and observed pH slope is done and the regression parameters are used to calculate proton flows from the pX slopes observed during the biological experiment. <br />
<br />
:::: These approaches assume a linear relationship between pH change and introduced protons. This is an approximation that is only valid for very small pH changes. In other words the buffering capacity has to be constant during the entire experiment.<br />
<br />
:::: For calculating proton production rates following this approach directly in DatLab, see below.<br />
<br />
==== From the amount of injected base ====<br />
<br />
:::: The limitations mentioned above can be overcome by using the amount of base necessary in the pH-Stat approach to hold the pH value constant. If the pH values at the beginning and at the end of a time interval are identical then the proton floe during this time interval can be directly calculated from the amount of base injected to keep the pH value constant. While this method does not assume a constant buffering capacity during the entire experiment there are some drawbacks: <br />
<br />
::::* Usually only the "pH-Stat-strict" approach will assure the identical pH values at the beginning and the end of a time period necessary for this approach (This could possibly be overcome by more advanced data analysis). <br />
::::* Only one value for proton flow for each period between injections is calculated in contrast to the continuous recording facilitated by the first mentioned method. <br />
::::* The injected volumes have to be read out form TIP events. This can be partially automated in a spreadsheet template (pH Stat Template Injected Volume) but is more tedious than the method using the buffering capacity <br />
::::* Initial trials with simulated proton flows indicted this method to be slightly less precise than the method using the buffering capacity.<br />
<br />
==== Conclusion and templates ====<br />
<br />
:::: A potential compromise would be to use the method based on buffering capacity for routine calculations but check selected (late) phases of the experiment with the method based on added base volume. Thereby, significant changes in buffering capacity during the experiment should be detected. Spreadsheet (Excel) templates for both methods are available for download [[here|here]].<br />
<br />
=== DatLab analysis ===<br />
==== [[Image:Px referencelayouts.png|right|600 px]] pX reference layouts ====<br />
:::: '''Graph layout''': Four [[Layout for DatLab graphs|reference layouts]] are available in [[DatLab 7]] based on the recorded pX signal: <br />
:::::*'''01 Potentiometric'''<br />
:::::*'''02a TPP_calibration'''<br />
:::::*'''02b TPP_with_O2flux'''<br />
:::::*'''02c TPP_calibrated_with_O2flux'''<br />
:::: These layouts can be selected in [Layout / Reference layouts / O2 & pX].<br />
<br />
:::: '''Reference layouts''' can be modified and saved as user-defined layouts, see [[MiPNet19.18C DatLab Guide]].<br />
<br />
<br />
:::: [[Image:O2kcontrol px.PNG|400 px|right]]<br />
<br />
==== pX settings ====<br />
<br />
:::: In the '''[[O2k configuration]] window''' the pX channel can be activated and a label for the inserted pX electrode can be entered for documentation purposes.<br />
:::: '''Gain''' and '''Offset voltage [mV]''' for the pX channel can be set in the '''[[O2k control]] window''' [F7], tab: '''Potentiometric, pX'''. The gain influences the "pX Raw Signal” recorded in DatLab. Therefore, a gain of 1 will give the same voltage [V] as would be measured with any multimeter between reference and measuring electrode.<br />
<br />
<br />
<br />
==== pX calibration ====<br />
<br />
:::: From the Calibration menu the '''pX Calibration window''' window [Potentiometric, pX] is opened. This feature allows for a simple two-point linear calibration of pX (or –pX) as a function of recorded voltage, using data ranges marked for calibration [Select marks] or known pX values, see [[MiPNet19.18D_O2k-calibration| O2k-calibration]] and [[MiPNet08.16_pH_calibration| pH calibration]].<br />
<br />
<br />
:::: '''Calibrations for different signal types: '''There is only one set of calibration values for each pX channel, irrespective of the connected electrode. If a pX channel was calibrated for a pH electrode, these values will initially also be used to calculate the calibrated signal when the pH electrode is exchanged for a TPP<sup>+</sup> electrode. Even when observing only the raw (not the calibrated) signal, the time derivative (Slope pX) will be calculated from the calibrated signal, which might lead to confusion when the time derivative is used to access stability or signal drift. It is, therefore, suggested to set the calibrated signal to the raw signal whenever the raw signal is to be used as the primary data source.<br />
<br />
:::: [[Image:PX calibration window.JPG| right|500 px]] Calibration values from other files can be imported with '''Copy from file''' in the pX calibration window.<br />
<br />
<br />
==== Calculate proton production in DatLab ====<br />
:::: Note: Requires DatLab 5 or [[DatLab 6]]<br />
<br />
:::: Proton production rates can be calculated in real-time during data acquisition. Select the menu [Flux/Slope]/[Proton Flux].<br />
::::# Determining the buffering capacity of the medium: <br />
::::## Calibrate the pH electrode, observe the calibrated pH signal.<br />
::::## Fill TIP syringes with diluted acid or base.<br />
::::## Start a slow injection of acid or base into the media (no sample present).<br />
::::## Place a mark on a stable region of the slope plot of the calibrated pH signal 'pX slope".<br />
::::## Go to [Plots]/[Proton Flux].<br />
::::## The buffering capacity is calculated by DatLab and can be used for calculations of biological proton flow in the same file or noted down and used in subsequent experiments. <br />
::::# Biological proton flux:<br />
::::## Calibrate the pH electrodes.<br />
::::## Observe the pH calibrated signal.<br />
::::## Place marks on regions of interest on the pX slope plot. If you use the "pH" stat" se above to keep the pH value in desired limit make sure that you exclude times during which base was injected.<br />
::::## Select [Flux/Slope] / [Proton Flux].<br />
::::## Enter the buffering capacity in the appropriate field or use the feature in the upper part of the window to calculate the buffering capacity from a calibration experiment in the same file.<br />
::::## Press [OK].<br />
::::## A new plot "Proton Flux" is now available in [Graph]/[Select Plots] (right at the end of the list. You can now chose to display this plot e.g. instead of the pX slope plot by selecting its check box and de-selecting the check box of the "pX slope" plot.<br />
<br />
:::: Known issues: DatLab always calculates a new buffering capacity from the input in the upper part of the window and does not remember the value from previous files. Therefore, if the determination of buffering capacity was done in a different Datlab file the value has to manually entered.<br />
<br />
<br />
== Specifications ==<br />
<br />
:::: Specifications provided by OROBOROS INSTRUMENTS for quality control of pH electrodes: <br />
::::* Drift (after 45 min stabilization, integrated over 5 minutes, 37 °C, 2 mM buffering capacity): <= 20 µpH/s.<br />
<br />
== pH calibration: temperature dependence ==<br />
<br />
:::: [[MiPNet08.16 pH calibration]].<br />
<br />
:::: While working with the pX channel please always observe the guidelines for avoiding damage to the electronics by [[ESD]].<br />
<br />
:::: pH electrodes have to be calibrated at the temperature intended for use and the correct pH values of the pH calibration buffer has to be used for the calibration. These values are sometimes difficult to come by. Sigma-Aldrich supplied us with temperature dependent pH values for the following products:&nbsp; <br />
<br />
{| cellspacing="1" cellpadding="1" border="1" style="width: 418px; height: 313px;"<br />
|-<br />
! scope="col" | T<br> <br />
! scope="col" | B5020 (pH4)<br> <br />
! scope="col" | B4770 (pH 7)<br> <br />
! scope="col" | B4985 (pH 10)<br><br />
|-<br />
| °C<br> <br />
| pH<br> <br />
| pH<br> <br />
| pH<br><br />
|-<br />
| 0<br> <br />
| 4.01<br> <br />
| 7.12<br> <br />
| 10.31<br><br />
|-<br />
| 5<br> <br />
| 4.01<br> <br />
| 7.09<br> <br />
| 10.23<br><br />
|-<br />
| 10<br> <br />
| 4.00<br> <br />
| 7.06<br> <br />
| 10.17<br><br />
|-<br />
| 20<br> <br />
| 4.00<br> <br />
| 7.02<br> <br />
| 10.05<br><br />
|-<br />
| 25<br> <br />
| 4.01<br> <br />
| 7.00<br> <br />
| 10.00<br><br />
|-<br />
| 30<br> <br />
| 4.01<br> <br />
| 6.99<br> <br />
| 9.95<br><br />
|-<br />
| 35<br> <br />
| 4.02<br> <br />
| 6.98<br> <br />
| 9.91<br><br />
|-<br />
| 40<br> <br />
| 4.03<br> <br />
| 6.98<br> <br />
| 9.87<br><br />
|-<br />
| 45<br> <br />
| 4.04<br> <br />
| 6.97<br> <br />
| <br><br />
|-<br />
| 50<br> <br />
| 4.06<br> <br />
| 6.97<br> <br />
| 9.81<br><br />
|-<br />
| 55<br> <br />
| 4.08<br> <br />
| <br> <br />
| <br><br />
|-<br />
| <br> <br />
| <br> <br />
| <br> <br />
| <br><br />
|}<br />
<br> <br />
<br />
::::(a) There seems to be a contradiction between the value stated for the pH 4 buffer at 25 °C in this table and on the product itself ("pH 4.00 +-1").<br />
::::(b) This information is as supplied to OROBOROS several years ago and with no responsibility by Sigma Aldrich for the correctness of this information.<br />
<br />
== pH-Stat ==<br />
<br />
:::: One approach we have developed is to use the [[TIP]] to run in a "pH stat" mode, i.e. keeping the pH constant by a feedback controlled automatic injection of base. Besides keeping the pH in the desired range this can actually be used to determine proton flow from the amount of base injected, circumventing the determination of buffering capacity, see below. The "pH-Stat" allows using very weakly buffered media (2 mM buffering substances) and might even make buffering obsolete. The feedback modus of the [[TIP]] can be used in two ways to achieve a pH-Stat modus: <br />
<br />
::::* pH-Stat_strict: This program keeps the pH value strictly between user defined upper and lower limits. The difference between the upper and lower limit will determine the time between injections depending on the current proton flow. Therefore, the time between injections may vary drastically with changing proton fluxes.<br />
<br />
<br> <br />
<br />
::::* pH-Stat_interval: This program adjusts the pH value in certain time intervals back to the upper limit. The difference between the upper and lower limit is set extremely small but a defined minimum pause between injections of e.g. 180 s is defined. Therefore, usually a base injection will be done every 3 minutes and the pH value will oscillate between the upper limit and some (proton flux dependent) lower limit. The lower limit set in the program has no significance because the minimum pause time will not have elapsed when the lower limit is met.<br />
<br />
:::: While the pH-Stat_strict is necessary to keep the pH value in a precisely defined range the pH-Stat_interval program ensures defined periods undisturbed by any injection of base. Such periods are necessary for the calculation of proton flow from the observed pH change and theoretically also for measuring respiration (however, if a 100 mM KOH the disturbance of the oxygen signal by the small amounts of KOH added was usually very small). <br />
<br />
:::: TIP2k setups in the DatLab template file '''DLTemplates_pH.dlt''' and Spreadsheet (e.g. Excel) templates for determining proton form base injections are available for download [http://www.oroboros.at/?ph-oxygen here]. Please note that of course the more straightforward calculation of proton flows from the measured pH slope is also possible while operating in pH-Stat mode! <br />
<br />
== Applications ==<br />
<br />
:::: For simultaneous measurement of O2 and pH, we refer to the classical literature on bioenergetics and the discovery of the chemiosmotic coupling mechanism, the quantification of H+/O2 stoichiometric ratios for proton pumping (Peter Mitchell). Other groups (e.g. [[SE_Lund_Elmer E]]) have used the pH electrode in the O2k in conjunction with a study of mitochondrial permeability transition. <br />
<br />
:::: The majority of novel applications will address the problem of aerobic glycolysis in intact cells, using the measurement of proton production as an indirect but continuous record of lactate production and corresponding acidification of the medium, while simultaneously monitoring oxygen concentration and oxygen consumption. In a well buffered culture medium, the pH change is extremely small relative to the amount of protons (lactic acid) produced, hence a low-buffering capacity medium needs to be applied. A titration of acid (lactic acid or HCl) into the low-buffering capacity medium yields the pH-dependent buffering capacity (Delta H+ added/Delta H+ measured by the pH electrode). Under various metabolic conditions, lactic acid production is the dominant mechanism causing acidification, hence the pH measurement is a good indirect indicator of aerobic glycolysis. <br />
<br />
<br />
== References ==<br />
::::* [[Talk:O2k-pH ISE-Module|Oxygen and pH - Warburg versus Crabtree Effect]]<br />
<br />
::::* '''O2k-Core Manual''': [[Media:MiPNet19.18 O2k-Core Manual.pdf|Contents: O2k-Core Manual.pdf]]<br />
{{#ask:[[Category:Publications]] [[Instrument and method::O2k-Manual]] [[Additional label::O2k-Core]]<br />
| mainlabel=Chapter<br />
|?Has title=Section<br />
|?Was published in year=Last update<br />
|format=broadtable<br />
|limit=500<br />
|sort=<br />
|order=ascending<br />
|offset=0<br />
}}<br />
<br />
[[Image:Titration-Injection-microPump.jpg|150px|left|link=http://www.bioblast.at/index.php?title=TIP2k-Module]]<br />
::::* '''TIP2k-Manual***<br />
::::» [[O2k-Catalogue: TIP2k]]<br />
::::» [[O2k-Publications: TIP2k]]<br />
{{#ask:[[Category:Publications]] [[Instrument and method::O2k-Manual]] [[Instrument and method::TIP2k]]<br />
| mainlabel=Chapter<br />
|?Has title=Section<br />
|?Was published in year=Last update<br />
|format=broadtable<br />
|limit=500<br />
|sort=<br />
|order=ascending<br />
|offset=0<br />
}}<br />
<br />
<br />
:::'''O2k-Protocols'''<br />
::::* [[MiPNet14.06 Instrumental O2 background]]<br />
::::* [[MiPNet08.16 pH calibration]]</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=ROUTINE_respiration&diff=135153ROUTINE respiration2017-05-04T08:50:56Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=''R''<br />
|description=[[File:R.jpg]] In the intact cell, '''ROUTINE respiration''' or '''ROUTINE activity''' in the physiological coupling state ''R'', is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation (intrinsic [[uncoupling]] and pathological [[dyscoupling]]). The conditions for measurement and expression of respiration vary ([[oxygen flux]] in state ''R'', ''J''<sub>O2''R''</sub> or [[oxygen flow]] in state ''R'', ''I''<sub>O2''R''</sub>). If these conditions are defined and remain consistent within a given context, then the simple symbol ''R'' for respiratory state can be used to substitute the more explicit expression for respiratory activity. ''R'' and growth of cells is supported by exogenous substrates in culture media. In media without energy substrates, ''R'' depends on endogenous substrates. ''R'' cannot be measured in [[permeabilized cells]] or [[isolated mitochondria]]. ''R'' is corrected for [[residual oxygen consumption]] (ROX), whereas ''R''´ is the uncorrected apparent ROUTINE respiration or total cellular oxygen consumption of cells including ROX.<br />
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger_2008_POS]]<br />
}}<br />
{{MitoPedia concepts<br />
|mitopedia concept=Respiratory state, Recommended<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=EAGLE<br />
}}<br />
Contributed by [[Gnaiger E]] 2010-09-18, edited 2014-08-25.<br />
[[File:ROUTINE.jpg|400px|thumb|Coupled energy cycles and and proton circuit of mitochondrial respiration in intact cells controlled by aerobic ATP demand in the ROUTINE state of activity. 2[H] indicates the reduced hydrogen equivalents of CHO substrates and electron transfer to oxygen, with a variety of energy substrates from carbohydrates through glycolysis to the tricarboxylic acid cycle (TCA) and glycerophosphate dehydrogenase complex (C<sub>GpDH</sub>), amino acid oxidation (AAO) and fatty acid oxidation (FAO through the electron transferring flavoprotein complex, C<sub>ETF</sub>). H<sup>+</sup><sub>out</sub> are protons pumped out of the matrix phase to the positive P-phase. Proton flow to the negative matrix phase (H<sup>+</sup><sub>in</sub>) drives the phosphorylation of ADP to ATP. Proton leaks dissipate energy of translocated protons from the positive P-phase to the negative N-phase (modified after [[Gnaiger 2012 MitoPathways]]).]]<br />
<br /><br />
<br /><br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[Basal respiration]]<br />
::::» [[Dyscoupling]]<br />
::::» [[Isolated mitochondria]]<br />
::::» [[LEAK respiration]]<br />
::::» [[Oxygen flow]] <br />
::::» [[Oxygen flux]] <br />
::::» [[Permeabilized cells]] <br />
::::» [[Residual oxygen consumption|ROX]]<br />
::::» [[Uncoupling]] <br />
</div><br />
</div><br />
<br /><br />
<br />
== Why not 'basal respiration', and what about 'State 3.5'? ==<br />
<br />
:::: '''Basal respiration''' or "basal metabolic rate" is frequently used as [[LEAK respiration]]. Importantly, we have to make a clear distinction between (oligomycin-inhibited) LEAK respiration and physiologically controlled [[ROUTINE respiration]], none of which conform to the physiological definition of [[basal respiration]].<br />
<br />
<br />
== Related terms in Bioblast ==<br />
:::: [[File:P.jpg |link=OXPHOS capacity]] [[OXPHOS capacity |OXPHOS]], ''P''<br />
<br />
:::: [[File:R.jpg |link=ROUTINE respiration]] [[ROUTINE respiration |ROUTINE]], ''R''<br />
<br />
:::: [[File:E.jpg |link=ETS capacity]] [[ETS capacity |ETS]], ''E''<br />
<br />
:::: [[File:L.jpg |link=LEAK respiration]] [[LEAK respiration |LEAK]], ''L''<br />
<br />
:::: [[File:ROX.jpg |link=Residual oxygen consumption]] [[Residual oxygen consumption |ROX]], ''R''<br />
<br />
<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» List of publications: ROUTINE'''</span><br />
<div class="mw-collapsible-content"><br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Coupling states::ROUTINE]]<br />
|?Was published in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway<br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
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}}<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
= Abstracts: ROUTINE =<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Coupling states::ROUTINE]]<br />
|?Was submitted in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway <br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
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}}<br />
</div><br />
</div><br />
<br /></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=ROUTINE_respiration&diff=135152ROUTINE respiration2017-05-04T08:49:11Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=''R''<br />
|description=[[File:R.jpg]] In the intact cell, '''ROUTINE respiration''' or '''ROUTINE activity''' in the physiological coupling state ''R'', is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation (intrinsic [[uncoupling]] and pathological [[dyscoupling]]). The conditions for measurement and expression of respiration vary ([[oxygen flux]] in state ''R'', ''J''<sub>O2''R''</sub> or [[oxygen flow]] in state ''R'', ''I''<sub>O2''R''</sub>). If these conditions are defined and remain consistent within a given context, then the simple symbol ''R'' for respiratory state can be used to substitute the more explicit expression for respiratory activity. ''R'' and growth of cells is supported by exogenous substrates in culture media. In media without energy substrates, ''R'' depends on endogenous substrates. ''R'' cannot be measured in [[permeabilized cells]] or [[isolated mitochondria]]. ''R'' is corrected for [[residual oxygen consumption]] (ROX), whereas ''R''´ is the uncorrected apparent ROUTINE respiration or total cellular oxygen consumption of cells including ROX.<br />
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger_2008_POS]]<br />
}}<br />
{{MitoPedia concepts<br />
|mitopedia concept=Respiratory state, Recommended<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=EAGLE<br />
}}<br />
Contributed by [[Gnaiger E]] 2010-09-18, edited 2014-08-25.<br />
[[File:ROUTINE.jpg|400px|thumb|Coupled energy cycles and and proton circuit of mitochondrial respiration in intact cells controlled by aerobic ATP demand in the ROUTINE state of activity. 2[H] indicates the reduced hydrogen equivalents of CHO substrates and electron transfer to oxygen, with a variety of energy substrates from carbohydrates through glycolysis to the tricarboxylic acid cycle (TCA) and glycerophosphate dehydrogenase complex (C<sub>GpDH</sub>), amino acid oxidation (AAO) and fatty acid oxidation (FAO through the electron transferring flavoprotein complex, C<sub>ETF</sub>). H<sup>+</sup><sub>out</sub> are protons pumped out of the matrix phase to the positive P-phase. Proton flow to the negative matrix phase (H<sup>+</sup><sub>in</sub>) drives the phosphorylation of ADP to ATP. Proton leaks dissipate energy of translocated protons from the positive P-phase to the negative N-phase (modified after [[Gnaiger 2012 MitoPathways]]).]]<br />
<br /><br />
<br /><br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[Basal respiration]]<br />
::::» [[Dyscoupling]]<br />
::::» [[Isolated mitochondria]]<br />
::::» [[LEAK respiration]]<br />
::::» [[Oxygen flux]] <br />
::::» [[Oxygen flow]] <br />
::::» [[Permeabilized cells]] <br />
::::» [[Residual oxygen consumption|ROX]]<br />
::::» [[Uncoupling]] <br />
</div><br />
</div><br />
<br /><br />
<br />
== Why not 'basal respiration', and what about 'State 3.5'? ==<br />
<br />
:::: '''Basal respiration''' or "basal metabolic rate" is frequently used as [[LEAK respiration]]. Importantly, we have to make a clear distinction between (oligomycin-inhibited) LEAK respiration and physiologically controlled [[ROUTINE respiration]], none of which conform to the physiological definition of [[basal respiration]].<br />
<br />
<br />
== Related terms in Bioblast ==<br />
:::: [[File:P.jpg |link=OXPHOS capacity]] [[OXPHOS capacity |OXPHOS]], ''P''<br />
<br />
:::: [[File:R.jpg |link=ROUTINE respiration]] [[ROUTINE respiration |ROUTINE]], ''R''<br />
<br />
:::: [[File:E.jpg |link=ETS capacity]] [[ETS capacity |ETS]], ''E''<br />
<br />
:::: [[File:L.jpg |link=LEAK respiration]] [[LEAK respiration |LEAK]], ''L''<br />
<br />
:::: [[File:ROX.jpg |link=Residual oxygen consumption]] [[Residual oxygen consumption |ROX]], ''R''<br />
<br />
<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» List of publications: ROUTINE'''</span><br />
<div class="mw-collapsible-content"><br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Coupling states::ROUTINE]]<br />
|?Was published in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway<br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=descending<br />
}}<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
= Abstracts: ROUTINE =<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Coupling states::ROUTINE]]<br />
|?Was submitted in year=Year<br />
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|?Coupling states=Coupling<br />
|?Pathways=Pathway <br />
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</div><br />
<br /></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=ROUTINE_respiration&diff=135151ROUTINE respiration2017-05-04T08:47:16Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=''R''<br />
|description=[[File:R.jpg]] In the intact cell, '''ROUTINE respiration''' or '''ROUTINE activity''' in the physiological coupling state ''R'', is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation (intrinsic [[uncoupling]] and pathological [[dyscoupling]]). The conditions for measurement and expression of respiration vary ([[oxygen flux]] in state ''R'', ''J''<sub>O2''R''</sub> or [[oxygen flow]] in state ''R'', ''I''<sub>O2''R''</sub>). If these conditions are defined and remain consistent within a given context, then the simple symbol ''R'' for respiratory state can be used to substitute the more explicit expression for respiratory activity. ''R'' and growth of cells is supported by exogenous substrates in culture media. In media without energy substrates, ''R'' depends on endogenous substrates. ''R'' cannot be measured in [[permeabilized cells]] or [[isolated mitochondria]]. ''R'' is corrected for [[residual oxygen consumption]] (ROX), whereas ''R''´ is the uncorrected apparent ROUTINE respiration or total cellular oxygen consumption of cells including ROX.<br />
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger_2008_POS]]<br />
}}<br />
{{MitoPedia concepts<br />
|mitopedia concept=Respiratory state, Recommended<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=EAGLE<br />
}}<br />
Contributed by [[Gnaiger E]] 2010-09-18, edited 2014-08-25.<br />
[[File:ROUTINE.jpg|400px|thumb|Coupled energy cycles and and proton circuit of mitochondrial respiration in intact cells controlled by aerobic ATP demand in the ROUTINE state of activity. 2[H] indicates the reduced hydrogen equivalents of CHO substrates and electron transfer to oxygen, with a variety of energy substrates from carbohydrates through glycolysis to the tricarboxylic acid cycle (TCA) and glycerophosphate dehydrogenase complex (C<sub>GpDH</sub>), amino acid oxidation (AAO) and fatty acid oxidation (FAO through the electron transferring flavoprotein complex, C<sub>ETF</sub>). H<sup>+</sup><sub>out</sub> are protons pumped out of the matrix phase to the positive P-phase. Proton flow to the negative matrix phase (H<sup>+</sup><sub>in</sub>) drives the phosphorylation of ADP to ATP. Proton leaks dissipate energy of translocated protons from the positive P-phase to the negative N-phase (modified after [[Gnaiger 2012 MitoPathways]]).]]<br />
<br /><br />
<br /><br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[Basal respiration]]<br />
::::» [[Dyscoupling]]<br />
::::» [[Isolated mitochondria]]<br />
::::» [[LEAK respiration]]<br />
::::» [[Oxygen flux]] <br />
::::» [[Oxygen flow]] <br />
::::» [[Permeabilized cells]] <br />
::::» [[Residual oxygen consumption|ROX]]<br />
::::» [[Uncoupling]] <br />
</div><br />
</div><br />
<br /><br />
<br />
== Why not 'basal respiration', and what about 'State 3.5'? ==<br />
<br />
:::: '''Basal respiration''' or "basal metabolic rate" is frequently used as [[LEAK respiration]]. Importantly, we have to make a clear distinction between (oligomycin-inhibited) LEAK respiration and physiologically controlled [[ROUTINE respiration]], none of which conform to the physiological definition of [[basal respiration]].<br />
<br />
<br />
== Related terms in Bioblast ==<br />
:::: [[File:P.jpg |link=OXPHOS capacity]] [[OXPHOS capacity |OXPHOS]], ''P''<br />
<br />
:::: [[File:R.jpg |link=ROUTINE respiration]] [[ROUTINE respiration |ROUTINE]], ''R''<br />
<br />
:::: [[File:E.jpg |link=ETS capacity]] [[ETS capacity |ETS]], ''E''<br />
<br />
:::: [[File:L.jpg |link=LEAK respiration]] [[LEAK respiration |LEAK]], ''L''<br />
<br />
:::: [[File:ROX.jpg |link=Residual oxygen consumption]] [[Residual oxygen consumption |ROX]], ''R''<br />
<br />
<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
= List of publications: ROUTINE =<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Coupling states::ROUTINE]]<br />
|?Was published in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway<br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=descending<br />
}}<br />
<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
= Abstracts: ROUTINE =<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Coupling states::ROUTINE]]<br />
|?Was submitted in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway <br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was submitted in year<br />
|order=descending<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=ADP&diff=135150ADP2017-05-04T08:33:38Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=D<br />
|description='''Adenosine diphosphate''' is a nucleotid. In [[OXPHOS]] core metabolism, ADP is a substrate of [[ANT]] and [[ATP synthase]] in the [[phosphorylation system]]. ADP is the discharged or low-energy counterpart of [[ATP]]. ADP can accept chemical energy by regaining a phosphate group to become ATP, in substrate-level phosphorylation (in anaerobic catabolism), at the expense of solar energy (in photosynthetic cells) or chemiosmotic energy (respiration in heterotrophic cells). ADP is added to [[mitochondrial preparations]] at kinetically saturating concentrations to induce the active state for evaluation of [[OXPHOS capacity]].<br />
|info=[[MiPNet03.02]], [[MiPNet09.12]]<br />
}}{{MitoPedia topics<br />
|mitopedia topic=Substrate and metabolite<br />
}}<br />
<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[ANT]] <br />
::::» [[ATP]]<br />
::::» [[ATP synthase]] <br />
::::» [[MiR06]]<br />
::::» [[Mitochondrial preparations]] <br />
::::» [[MiPNet09.12 O2k-Titrations|O2k-Titrations]]<br />
::::» [[OXPHOS]]<br />
::::» [[OXPHOS capacity]]<br />
::::» [[Phosphorylation system]]<br />
</div><br />
</div><br />
<br /><br />
<br />
== Application in [[HRR]] ==<br />
<br />
:::: '''D: ADP''' (Adenosine 5'diphosphate potassium salt, C<sub>10</sub>H<sub>15</sub>N<sub>5</sub>O<sub>10</sub>P<sub>2</sub>K), <br />
::::Merck 117105-1GM ('''Calbiochem''') FW = 501.3; store at 4 °C. Recommended due to lower ATP impurities, we use 'ADP-Mg' in [[AT_Innsbruck_OROBOROS |OROBOROS Lab]].<br />
<br />
<br />
:::: Alternative source: Sigma A 5285, 1 g, store at -20 °C; FW = 501.3.<br />
<br />
:::: <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span><br />
<br />
<br />
::: '''Preparation of 500 mM ADP stock solution''' (dissolved in H<sub>2</sub>O):<br />
<br />
::::# Weigh 501.3 mg of ADP = 1 mmol ADP<br />
::::# Add 1.2 ml H<sub>2</sub>O (ADP is not dissolved at this stage).<br />
::::# Neutralize with 5 M KOH (approx. 450 µl). ADP will dissolve after addition of KOH.<br />
::::# Check pH and adjust to 7 if necessary.<br />
::::# Adjust final volume to 2 ml and divide into 0.2 ml portions.<br />
::::# Store at -80 °C.<br />
<br />
<br />
::: '''Preparation of 500 mM ADP stock solution with 300 mM free Mg<sup>2+</sup>''' (dissolved in H<sub>2</sub>O):<br />
<br />
:::: To keep free [Mg<sup>2+</sup>] constant during respiration measurement in [[MiR06]] or MiR05, mix ADP with MgCl<sub>2</sub> (0.6 mol MgCl<sub>2</sub>/mol ADP).<br />
<br />
:::: '''MgCl<sub>2</sub>''' (Scharlau MA0036: MgCl<sub>2</sub>.6H<sub>2</sub>O, FW = 203.3)<br />
<br />
::::# Weigh 501.3 mg of ADP = 1 mmol ADP.<br />
::::# Add 1.2 ml H<sub>2</sub>O (ADP is not dissolved at this stage).<br />
::::# Neutralize with 5 M KOH (approx. 450 µl). ADP will dissolve after addition of KOH.<br />
::::# Add 121,98 mg MgCl<sub>2</sub>. White precipitate will occur, which will dissolve during 1-2 min stirring on magnetic stirrer at RT.<br />
::::# Check pH and adjust to 7 if necessary.<br />
::::# Adjust final volume to 2 ml and divide into 0.2 ml portions.<br />
::::# Store at -80 °C.<br />
<br />
<br />
:::: '''Comment''': In some cases it was observed, that after defreezing of the 'ADP-Mg' a white precipitate has formed again. If this is the case we recommend to prepare the ADP stock solution and a MgCl<sub>2</sub> solution (0.6 mol MgCl<sub>2</sub>/mol ADP) seperately and perform a titration of both solutions in immediate succession.<br />
<br />
<br />
::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]]<br />
<br />
::::* Titration volume: 4-20 µl using a 25 µl syringe (2 ml O2k-chamber).<br />
::::* Final concentration: 1-5 mM.<br />
<br />
<br />
== ADP dependence of respiration ==<br />
<br />
The assumption of linearity (linear regression of oxygen concentration over time) is frequently not valid for various reasons other than [[oxygen kinetics]]. In classical ‘State 3’, ADP levels are ‘high’ ([[Chance_1955_JBC-III|Chance and Williams, 1955]]), but not necessarily saturating ([[MitoPedia: Respiratory states]]). Then, an ADP-dependent decline of respiration is observed immediately after titration of a sub-saturating concentration of ADP, which is obscured by any linear regression. This has caused in the past a tremendous underestimation of the apparent Km for ADP, perpetuated even today with the uncritical application of non-adequate software implementing the simple linearity approach only. For a critical approach to ADP kinetics, see [[Gnaiger_2000_Proc_Natl_Acad_Sci_USA|Gnaiger et al 2000]] and [[Gnaiger_2001_Respir_Physiol|Gnaiger 2001]].</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=OXPHOS_capacity&diff=135149OXPHOS capacity2017-05-04T08:25:01Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=''P''<br />
|description=[[File:P.jpg]] '''OXPHOS capacity''' (''P'') is the respiratory capacity of mitochondria in the ADP-activated state of [[oxidative phosphorylation]], at saturating concentrations of [[ADP]] (possibly in contrast to [[State 3]]), inorganic phosphate, oxygen, and defined reduced substrates. <br />
» [[#OXPHOS (P) and State 3 |'''MiPNet article''']]<br />
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]]<br />
}}<br />
{{MitoPedia concepts<br />
|mitopedia concept=Respiratory state, SUIT concept, Recommended<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=EAGLE<br />
}}<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[ADP]] <br />
::::» [[Citrate synthase|CS]]<br />
::::» [[Dyscoupling]] <br />
::::» [[Flow]]<br />
::::» [[Flux control ratio]]<br />
::::» [[Free OXPHOS capacity]]<br />
::::» [[Gnaiger 2014 MitoPathways]]<br />
::::» [[Oxidative phosphorylation]]<br />
::::» [[Oxygen flow]]<br />
::::» [[Oxygen flux]] <br />
::::» [[P/E]]<br />
::::» [[State 3]]<br />
::::» [[Uncoupling]] <br />
</div><br />
</div><br />
<br /><br />
<br />
<br />
<br />
[[File:OXPHOS-NS.jpg|400px|thumb|Coupled energy cycles of oxidative phosphorylation stimulated by saturating concentrations of ADP and inorganic phosphate. 2[H] indicates the reduced hydrogen equivalents of CHO substrates (NS) and electron transfer to oxygen. H<sup>+</sup><sub>out</sub> are protons pumped out of the matrix phase to the positive P-phase. Proton flow to the negative matrix phase (H<sup>+</sup><sub>in</sub>) drives the phosphorylation of ADP to ATP. The capacity of the phosphorylation system may contribute to the limitation of flux. Proton leaks dissipate energy of translocated protons from the positive P-phase to the negative N-phase. Measurement of OXPHOS capacity is possible in mt-preparations supported by an ETS-competent substrate state, exemplifed as the NS-pathway (CI<small>&</small>II-linked substrate supply) ([[Gnaiger 2014 MitoPathways]]).]]<br />
<br />
__TOC__<br />
= OXPHOS (''P'') and State 3 =<br />
{{Publication<br />
|title=Gnaiger E (2014) OXPHOS (''P'') and State 3. Mitochondr Physiol Network 2016-07-15.<br />
|info=<br />
|authors=OROBOROS<br />
|year=2016<br />
|journal=MiPNet<br />
|abstract=[[File:P.jpg]] '''OXPHOS capacity''' (''P'') is the respiratory capacity of mitochondria in the ADP-activated state of [[oxidative phosphorylation]], at saturating concentrations of [[ADP]] (possibly in contrast to [[State 3]]), inorganic phosphate, oxygen, and defined reduced substrates. Since OXPHOS is partially coupled, intrinsic [[uncoupling]] and [[dyscoupling]] contribute to the control of flux in the OXPHOS state (State ''P''). Oxygen consumption in the OXPHOS state, ''P'', therefore, is partitioned into the [[free OXPHOS capacity]], ''≈P'', strictly coupled to phosphorylation, ''~P'', and nonphosphorylating LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''P'' = ''≈P''+''L<sub>P</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>P</sub>'', as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>P</sub>''. However, if the LEAK component increases with enzyme turnover during ''P'', the low enzyme turnover during ''L'' may counteract the effect of the higher Δ''p''<sub>mt</sub>. OXPHOS capacity is expressed (i) per mt-marker (O<sub>2</sub> [[flux]] per mt-protein, [[Citrate synthase|CS]], etc); if ETS capacity, ''E'', is used as a functional mitochondrial marker, then OXPHOS capacity is expressed as the ''[[P/E]]'' ratio ([[flux control ratio]]). (ii) OXPHOS capacity is expressed per tissue or cell mass, integrating mt-quantity (density) and mt-quality (O<sub>2</sub> flux). (iii) OXPHOS capacity is expressed per cell (O<sub>2</sub> [[flow]]), which then is a function of mt-density, mt-quality, and cell size. If conditions for measurement and expression of respiration vary, explicit symbols are used, expressing OXPHOS capacity as [[oxygen flux]] in state ''P'', ''J''<sub>O2''P''</sub> or as [[oxygen flow]] in state ''P'', ''I''<sub>O2''P''</sub>. If these conditions are defined and remain consistent within a given context, then the simple symbol ''P'' for respiratory state can be used to substitute the more explicit expression for respiratory activity.<br />
|mipnetlab=AT Innsbruck Gnaiger E<br />
}}<br />
{{Labeling<br />
|couplingstates=OXPHOS<br />
|instruments=Theory<br />
}}<br />
<br />
<br />
<br />
== Related terms in Bioblast ==<br />
:::: [[File:P.jpg |link=OXPHOS capacity]] [[OXPHOS capacity |OXPHOS]], ''P''<br />
<br />
:::: [[File:R.jpg |link=ROUTINE respiration]] [[ROUTINE respiration |ROUTINE]], ''R''<br />
<br />
:::: [[File:E.jpg |link=ETS capacity]] [[ETS capacity |ETS]], ''E''<br />
<br />
:::: [[File:L.jpg |link=LEAK respiration]] [[LEAK respiration |LEAK]], ''L''<br />
<br />
:::: [[File:ROX.jpg |link=Residual oxygen consumption]] [[Residual oxygen consumption |ROX]], ''R''<br />
<br />
::::* [[Free OXPHOS capacity]], ''≈P'' = ''P-L''<br />
::::* [[Free ROUTINE activity]], ''≈R'' = ''R-L''<br />
::::* [[Free ETS capacity]], ''≈E'' = ''E-L''<br />
::::* [[Excess E-P capacity |Excess ''E-P'' capacity]], ''ExP'' = ''E-P''<br />
::::* [[Excess E-R capacity |Excess ''E-R'' capacity]], ''ExR'' = ''E-R''<br />
<br />
<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» List of publications: OXPHOS'''</span><br />
<div class="mw-collapsible-content"><br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Coupling states::OXPHOS]]<br />
|?Was published in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway<br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=descending<br />
}}<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
= Abstracts: OXPHOS =<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Coupling states::OXPHOS]]<br />
|?Was submitted in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway <br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was submitted in year<br />
|order=descending<br />
}}<br />
</div><br />
</div><br />
<br /></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=OXPHOS_capacity&diff=135148OXPHOS capacity2017-05-04T08:19:13Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=''P''<br />
|description=[[File:P.jpg]] '''OXPHOS capacity''' (''P'') is the respiratory capacity of mitochondria in the ADP-activated state of [[oxidative phosphorylation]], at saturating concentrations of [[ADP]] (possibly in contrast to [[State 3]]), inorganic phosphate, oxygen, and defined reduced substrates. <br />
» [[#OXPHOS (P) and State 3 |'''MiPNet article''']]<br />
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]]<br />
}}<br />
{{MitoPedia concepts<br />
|mitopedia concept=Respiratory state, SUIT concept, Recommended<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=EAGLE<br />
}}<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[ADP]] <br />
::::» [[Citrate synthase|CS]]<br />
::::» [[Dyscoupling]] <br />
::::» [[Flow]]<br />
::::» [[Flux control ratio]]<br />
::::» [[Free OXPHOS capacity]]<br />
::::» [[Gnaiger 2014 MitoPathways]]<br />
::::» [[Oxidative phosphorylation]]<br />
::::» [[Oxygen flow]]<br />
::::» [[Oxygen flux]] <br />
::::» [[P/E]]<br />
::::» [[State 3]]<br />
::::» [[Uncoupling]] <br />
</div><br />
</div><br />
<br /><br />
<br />
<br />
<br />
[[File:OXPHOS-NS.jpg|400px|thumb|Coupled energy cycles of oxidative phosphorylation stimulated by saturating concentrations of ADP and inorganic phosphate. 2[H] indicates the reduced hydrogen equivalents of CHO substrates (NS) and electron transfer to oxygen. H<sup>+</sup><sub>out</sub> are protons pumped out of the matrix phase to the positive P-phase. Proton flow to the negative matrix phase (H<sup>+</sup><sub>in</sub>) drives the phosphorylation of ADP to ATP. The capacity of the phosphorylation system may contribute to the limitation of flux. Proton leaks dissipate energy of translocated protons from the positive P-phase to the negative N-phase. Measurement of OXPHOS capacity is possible in mt-preparations supported by an ETS-competent substrate state, exemplifed as the NS-pathway (CI<small>&</small>II-linked substrate supply) ([[Gnaiger 2014 MitoPathways]]).]]<br />
<br />
__TOC__<br />
= OXPHOS (''P'') and State 3 =<br />
{{Publication<br />
|title=Gnaiger E (2014) OXPHOS (''P'') and State 3. Mitochondr Physiol Network 2016-07-15.<br />
|info=<br />
|authors=OROBOROS<br />
|year=2016<br />
|journal=MiPNet<br />
|abstract=[[File:P.jpg]] '''OXPHOS capacity''' (''P'') is the respiratory capacity of mitochondria in the ADP-activated state of [[oxidative phosphorylation]], at saturating concentrations of [[ADP]] (possibly in contrast to [[State 3]]), inorganic phosphate, oxygen, and defined reduced substrates. Since OXPHOS is partially coupled, intrinsic [[uncoupling]] and [[dyscoupling]] contribute to the control of flux in the OXPHOS state (State ''P''). Oxygen consumption in the OXPHOS state, ''P'', therefore, is partitioned into the [[free OXPHOS capacity]], ''≈P'', strictly coupled to phosphorylation, ''~P'', and nonphosphorylating LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''P'' = ''≈P''+''L<sub>P</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>P</sub>'', as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>P</sub>''. However, if the LEAK component increases with enzyme turnover during ''P'', the low enzyme turnover during ''L'' may counteract the effect of the higher Δ''p''<sub>mt</sub>. OXPHOS capacity is expressed (i) per mt-marker (O<sub>2</sub> [[flux]] per mt-protein, [[Citrate synthase|CS]], etc); if ETS capacity, ''E'', is used as a functional mitochondrial marker, then OXPHOS capacity is expressed as the ''[[P/E]]'' ratio ([[flux control ratio]]). (ii) OXPHOS capacity is expressed per tissue or cell mass, integrating mt-quantity (density) and mt-quality (O<sub>2</sub> flux). (iii) OXPHOS capacity is expressed per cell (O<sub>2</sub> [[flow]]), which then is a function of mt-density, mt-quality, and cell size. If conditions for measurement and expression of respiration vary, explicit symbols are used, expressing OXPHOS capacity as [[oxygen flux]] in state ''P'', ''J''<sub>O2''P''</sub> or as [[oxygen flow]] in state ''P'', ''I''<sub>O2''P''</sub>. If these conditions are defined and remain consistent within a given context, then the simple symbol ''P'' for respiratory state can be used to substitute the more explicit expression for respiratory activity.<br />
|mipnetlab=AT Innsbruck Gnaiger E<br />
}}<br />
{{Labeling<br />
|couplingstates=OXPHOS<br />
|instruments=Theory<br />
}}<br />
<br />
<br />
<br />
== Related terms in Bioblast ==<br />
:::: [[File:P.jpg |link=OXPHOS capacity]] [[OXPHOS capacity |OXPHOS]], ''P''<br />
<br />
:::: [[File:R.jpg |link=ROUTINE respiration]] [[ROUTINE respiration |ROUTINE]], ''R''<br />
<br />
:::: [[File:E.jpg |link=ETS capacity]] [[ETS capacity |ETS]], ''E''<br />
<br />
:::: [[File:L.jpg |link=LEAK respiration]] [[LEAK respiration |LEAK]], ''L''<br />
<br />
:::: [[File:ROX.jpg |link=Residual oxygen consumption]] [[Residual oxygen consumption |ROX]], ''R''<br />
<br />
::::* [[Free OXPHOS capacity]], ''≈P'' = ''P-L''<br />
::::* [[Free ROUTINE activity]], ''≈R'' = ''R-L''<br />
::::* [[Free ETS capacity]], ''≈E'' = ''E-L''<br />
::::* [[Excess E-P capacity |Excess ''E-P'' capacity]], ''ExP'' = ''E-P''<br />
::::* [[Excess E-R capacity |Excess ''E-R'' capacity]], ''ExR'' = ''E-R''<br />
<br />
<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
= List of publications: OXPHOS =<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Coupling states::OXPHOS]]<br />
|?Was published in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway<br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=descending<br />
}}<br />
<br />
<br />
<br />
[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
= Abstracts: OXPHOS =<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Coupling states::OXPHOS]]<br />
|?Was submitted in year=Year<br />
|?Has title=Reference<br />
|?Coupling states=Coupling<br />
|?Pathways=Pathway <br />
|?Preparation=Preparation<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was submitted in year<br />
|order=descending<br />
}}</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=O2k-Specifications&diff=135147O2k-Specifications2017-05-04T08:05:43Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{Product<br />
|description='''O2k versus multiwell respirometer''': '''O2k''' stands for Oxygraph-2k and '''high-resolution respirometry''', meeting powerful quality criteria securing '''high output''' and pioneering state-of-the-art [[Gnaiger_2012_MitoPathways|comprehensive OXPHOS analysis]] of substrate control and coupling control of mitochondrial function. 'High throughput' stands for disposable multiwell systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is not equivalent to high output. ''If you’re using a biased instrument, it doesn’t matter how many measurements you take – you’re aiming at the wrong target'' ([[Silver 2012 Penguin Press]]).<br />
|product id=* [[Media:MiPNet18.10 O2kvsMultiwell.pdf|'''Open Access: O2k vs multiwell.pdf''']]<br />
|product type=O2k, Feedback<br />
|info=[[MiPNet18.10 O2k versus multiwell|Bioblast pdf]]<br />
|product image=[[Image:O2k-Info.png|right|30px|link=Oxygraph-2k]]<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware<br />
}}<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[Gnaiger 2012 MitoPathways|Comprehensive OXPHOS analysis]]<br />
::::» [[High-output, low running costs]]<br />
::::» [[O2k-Publications: Topics]]<br />
::::» [[Power-O2k]]<br />
::::» [[Specifications of the O2k]]'''<br />
</div><br />
</div><br />
<br /><br />
<br />
<br />
{{Publication<br />
|title=[[Image:Logo OROBOROS INSTRUMENTS.jpg|right|60px|link=OROBOROS INSTRUMENTS]] O2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis.<br />
|info= [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/5/54/MiPNet18.10_O2kvsMultiwell.pdf|Bioblast pdf]] <br />
|authors=OROBOROS<br />
|year=2015-10-05<br />
|journal=MiPNet<br />
|abstract='''Gnaiger E, Fasching M (2015) O2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(06): 1-8.''' » [http://www.bioblast.at/index.php/File:MiPNet18.10_O2kvsMultiwell.pdf Versions]<br />
|keywords=O2k-Specifications<br />
|mipnetlab=AT Innsbruck OROBOROS<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Human<br />
|tissues=Fibroblast<br />
|preparations=Intact cells, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP<br />
|instruments=Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, Theory<br />
}}<br />
== Discussion ==<br />
:::: Scientists who have experience with both instruments are invited to extend the discussion:<br />
<br />
::::» [[Microwell respirometry]]<br />
::::» [[Talk:O2k_versus_multiwell_respirometer#Tony_Hickey:_Calibration_is_my_chief_concern |'''Tony Hickey: Calibration is my chief concern''']]<br />
::::» .. and more: [[Talk:O2k versus multiwell respirometer]]<br />
::::» [[Talk:Rogers 2011 PLoS One|XFe for isolated mitochondria - a critical discussion]]<br />
<br />
<br />
== Combination of interests ==<br />
:::: [[Gnaiger E|Erich Gnaiger]] is founder and CEO of OROBOROS INSTRUMENTS, and responsible for the development of the O2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations.<br />
<br />
<br />
== Selected references ==<br />
<br />
::::* Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. [[Gnaiger 1995 J Bioenerg Biomembr |»Bioblast pdf«]]<br />
<br />
::::* Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. [[Steinlechner-Maran_1996_Am J Physiol Cell Physiol |»Bioblast pdf«]]<br />
<br />
::::* Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5. [[Gnaiger 2000 Proc Natl Acad Sci U S A |»Open Access«]]<br />
<br />
::::* Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. [[Gnaiger 2001 Respir Physiol |»Bioblast pdf«]]<br />
<br />
::::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. [[Huetter_2004_Biochem J |»Open Access«]]<br />
<br />
::::* Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome ''c'' release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286:H1633–41. [[Kuznetsov_2004_Am_J_Physiol_Heart_Circ_Physiol |»Open Access«]]<br />
<br />
::::* Aragonés J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40:170-80. [[Aragones 2008 Nat Genet |»Open Access«]]<br />
<br />
::::* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |»Bioblast pdf«]]<br />
<br />
::::* Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. [[Scandurra 2010 Adv Exp Med Biol |»Bioblast pdf«]]<br />
<br />
::::* Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. [[Aguirre 2010 Biochim Biophys Acta ||»Bioblast pdf«]]<br />
<br />
::::* Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. [[Votion 2012 PLoS One ||»Open Access«]]<br />
<br />
::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast pdf«]] <br />
<br />
::::* Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »[[Gnaiger 2014 MitoPathways |Bioblast pdf«]]<br />
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[[Category:OroboPedia]]<br />
[[Category:MitoPedia]]</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Oroboros_O2k&diff=135146Oroboros O2k2017-05-04T08:00:30Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{Product<br />
|description='''OROBOROS O2k''' ('''Oxygraph-2k''') - the modular system for [[high-resolution respirometry]] ('''HRR'''). The O2k is the [[O2k-Core|2-chamber high-resolution respirometer]] for monitoring oxygen consumption with small amounts of biological samples. The modular O2k-concept yields a high flexibility for HRR, with the [[O2k-Core]] as the basis, and O2k-Modules supported by the O2k-Core. O2k-Modules are the Titration-Injection microPump ([[TIP2k-Module]]) and [[O2k-Catalogue:_O2k-MultiSensor|O2k-MultiSensor Modules]] ([[O2k-Fluorometer]]; Ion Selective Electrodes, ISE) <br />
|product id=1'''####'''<br />
|product type=O2k, Catalogue<br />
|info=[[OROBOROS O2k-Catalogue |O2k-Catalogue]], [[Gnaiger_2008_POS]]<br />
|product image=[[Image:O2k.jpg|200px|right]]<br />
}}<br />
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::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[O2k-Core|2-chamber high-resolution respirometer]] <br />
::::» [[High-resolution respirometry]] <br />
::::» [[HRR-Dissection Set]]<br />
::::» [[Microbalance-Set|Microbalance]]<br />
::::» [[O2k-Core]] <br />
::::» [[O2k-Fluorometer]]<br />
::::» [[O2k-Catalogue:_O2k-MultiSensor|O2k-MultiSensor Modules]]<br />
::::» [[PBI-Shredder HRR-Set]]<br />
::::» [[TIP2k-Module]]<br />
</div><br />
</div><br />
<br /><br />
<br />
<br />
<br />
== '''O2k-Catalogue: O2k-Modules and Auxiliary HRR-Accessories''' ==<br />
{{#ask: [[Category:Products]] [[Product type::O2k-Module]]<br />
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[[File:Oxygraph-2k-Concept.jpg|300px|right|link=O2k high-resolution respirometry |O2k and HRR]]<br />
<br /><br />
<br />
<big><big>'''The concept »[[O2k high-resolution respirometry]]'''</big></big><br />
<br /><br />
<br /><br />
<br />
== OROBOROS O2k: development and distribution ==<br />
<br />
:::: The O2k is developed by [[OROBOROS INSTRUMENTS]] in cooperation with [http://www.wgt.at WGT] and produced in Austria by WGT.<br />
<br />
:::: The O2k-Modules can be combined in any configuration with the O2k-Core. The modules can be added any time to extend the Oxygraph-2k as an O2k-MultiSensor System according to application-specific objectives.<br />
<br />
:::: Auxiliary HRR-Tools provide extended support in the context of high-resolution respirometry: [[PBI-Shredder HRR-Set]] for tissue preparation; [[Microbalance-Set|Microbalance]], [[HRR-Dissection Set]].</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Mitochondrial_marker_enzymes&diff=135145Mitochondrial marker enzymes2017-05-04T07:50:16Z<p>Bitschnau Barbara: </p>
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<div>{{MitoPedia<br />
|description='''Mitochondrial marker enzymes''' are enzymes that are specifically present in mitochondria, in the mt-matrix, the inner mt-membrane, the inter-membrane space, or the outer mt-membrane.<br />
|info=[[Gear 1970 Biochem J]]<br />
|type=Enzyme<br />
}}<br />
{{MitoPedia methods|type=Enzyme<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=Enzyme<br />
|type=Enzyme<br />
}}<br />
::::* [[Citrate synthase]] (mt-matrix)<br />
::::* NAD<sup>+</sup> [[malate dehydrogenase]] (mt-matrix)<br />
::::* NAD<sup>+</sup> [[glutamate dehydrogenase]] (mt-matrix)<br />
::::* Succinate cytochrome ''c'' reductase (inner mt-membrane)<br />
::::* Rotenone-sensitive NADH cytochrome ''c'' reductase (inner mt-membrane)<br />
::::* [[Adenylate kinase]] (intermembrane space)<br />
::::* Rotenone-insensitive NADH cytochrome ''c'' reductase (outer mt-membrane)<br />
::::* [[Monoamine oxidase]] (outer mt-membrane)<br />
::::* [[Kynurenine hydroxylase]] (outer mt-membrane)<br />
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[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
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<br /></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=LEAK_respiration&diff=135144LEAK respiration2017-05-04T07:36:32Z<p>Bitschnau Barbara: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=''L''<br />
|description=[[File:L.jpg]] '''LEAK respiration''' or LEAK oxygen flux, ''L'', compensating for [[proton leak]], [[proton slip]], cation cycling and [[electron leak]], is a dissipative component of respiration which is not available for performing biochemical work and thus related to heat production. LEAK respiration is measured in the LEAK state, in the presence of reducing substrate(s), but absence of ADP (theoretically, absence of inorganic phosphate presents an alternative), or after enzymatic inhibition of the [[phosphorylation system]]. The '''LEAK state''' is the non-phosphorylating resting state of intrinsic [[Uncoupler|uncoupled]] or [[Dyscoupled respiration|dyscoupled respiration]] when oxygen flux is maintained mainly to compensate for the proton leak at a high chemiosmotic potential, when ATP synthase is not active. In this non-phosphorylating resting state, the electrochemical proton gradient is increased to a maximum, exerting feedback control by depressing oxygen flux to a level determined mainly by the proton leak and the H<sup>+</sup>/O<sub>2</sub> ratio. In this state of maximum protonmotive force, LEAK respiration is higher than the LEAK component in state ''P'' ([[OXPHOS capacity]]). The conditions for measurement and expression of respiration vary ([[oxygen flux]] in state ''L'', ''J''<sub>O2''L''</sub> or [[oxygen flow]] in state ''L'', ''I''<sub>O2''L''</sub>). If these conditions are defined and remain consistent within a given context, then the simple symbol ''L'' for respiratory state can be used as a substitute for the more explicit expression for respiratory activity.<br />
» [[LEAK_respiration#LEAK respiration: concept-linked terminology of respiratory states |'''MiPNet article''']]<br />
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]]<br />
}}<br />
{{MitoPedia concepts<br />
|mitopedia concept=Respiratory state, Recommended<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=EAGLE<br />
}}<br />
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<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[ADP]]<br />
::::» [[Dyscoupled respiration]] <br />
::::» [[Electron leak]]<br />
::::» [[ETS capacity]]<br />
::::» [[LEAK state]]<br />
::::» [[LEAK state with ATP]]<br />
::::» [[LEAK state with Omy|LEAK state with oligomycin]]<br />
::::» [[LEAK state without adenylates]]<br />
::::» [[OXPHOS capacity]]<br />
::::» [[Oxygen flow]]<br />
::::» [[Oxygen flux]] <br />
::::» [[Phosphorylation system]]<br />
::::» [[Proton leak]]<br />
::::» [[Proton slip]]<br />
::::» [[ROX]]<br />
::::» [[State 1]]<br />
::::» [[State 2]]<br />
::::» [[State 3]]<br />
::::» [[State 4]]<br />
::::» [[State 4|State 4o]]<br />
::::» [[Static head]]<br />
::::» [[Uncoupler]]<br />
</div><br />
</div><br />
<br /><br />
__TOC__<br />
= LEAK respiration: concept-linked terminology of respiratory states =<br />
[[File:LEAK-NS.jpg|400px|thumb|LEAK respiration corresponds to resting, non-phosphorylating electron transfer with a shortcircuit of the proton cycle across the inner mt-membrane due to intrinsic uncoupling or dyscoupling. 2[H] indicates the reduced hydrogen equivalents of CHO substrates and electron transfer to oxygen. H<sup>+</sup><sub>out</sub> are protons pumped out of the matrix phase. Proton leaks (a property of the inner mt-membrane) dissipate energy of translocated protons, and proton slip prevents full translocation of protons across the inner mt-membrane (a property of the proton pumps). Measurement of LEAK respiration is possible in intact cells by inhibition of the phosphorylation system and in mt-preparations supported by an ETS-competent substrate state, exemplifed as the NS-pathway (CI<small>&</small>II-linked substrate supply). Modified after [[Gnaiger 2014 MitoPathways]].]]<br />
{{Publication<br />
|title=Gnaiger E (2014) LEAK respiration: concept-linked terminology of respiratory states. Mitochondr Physiol Network 2014-07-04.<br />
|info=[[Gnaiger 2014 MitoPathways]]<br />
|authors=OROBOROS<br />
|year=2014<br />
|journal=MiPNet<br />
|abstract=[[File:L.jpg]] Mitochondrial respiratory states have been defined originally by Chance and Williams (1955) as a sequence (from 1 to 5) of titrations and transitions in a respiratory protocol, including [[State 4]] as a '''[[LEAK state]]''' of respiration obtained after exhaustion of the added [[ADP]]. The second state ([[State 2]]) is induced by addition of 'high ADP'. Confusion persists in the current literature as to the meaning of State 2, which can be resolved by a transition from a specific protocol-linked to a generalized '''concept-linked terminology'''. <br />
|mipnetlab=AT Innsbruck Gnaiger E<br />
}}<br />
{{Labeling<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, ETS<br />
|instruments=Theory<br />
}}<br />
<br />
== The LEAK state ==<br />
:::: LEAK states of respiration are frequently called [[State 4]], [[State 4o]], basal state, or inactive state. Importantly, [[State 2]] is not a LEAK state according to the classical definition. <br />
<br />
:::: Respiration compensating for the [[proton leak]] is the main component of LEAK respiration. If proton leak were the only component involved, it would make sense to simply write leak respiration for the compensatory oxygen flux. [[Proton slip]] and cation cycling, however, are also involved to a larger or smaller extent to stimulate LEAK respiration. The upper case 'LEAK', therefore, should make us aware that this is more accurately considered as an acronym, and cannot be taken as a definitive delineation of the stimulatory mechanism in the LEAK state of respiration.<br />
<br />
::::* ''Note'': The distinction between LEAK and ETS capacity helps to clarify the important difference between physiologically uncoupled or pathologically dyscoupled respiration (LEAK) in contrast to noncoupled respiration ([[ETS capacity]]).<br />
<br />
<br />
<br />
== Protocols for measurement of LEAK respiration ==<br />
:::: The LEAK state can be induced experimentally in various ways, which may yield idential estimates of LEAK respiration, or may show deviations that help to critically assess the proper protocol to be applied in specific cases:<br />
<br />
=== a) LEAK state with ATP ===<br />
[[File:LEAK_T.jpg|left|300px|thumb|LEAK respiration in the presence of ATP, ''L''<sub>T</sub>, and absence of ATPase activity.]] [[LEAK state with ATP]]: ''L''<sub>T</sub> (classical [[State 4]] in isolated mitochondria) after phosphorylation of ADP to ATP is completed, or when a high concentration of ATP is added in the absence of ADP ([[Gnaiger 2000 Proc Natl Acad Sci U S A]]).<br />
<br />
:::: In contradiction to the original definition of State 2 ([[ROX]]), yet with reference to Chance and Williams (1956), 'State 2' has later been used for describing this functionally different state of [[LEAK respiration]]:<br />
<br />
:::‘''State 2: substrate added, respiration low due to lack of ADP. .. the controlled respiration prior to addition of ADP, which is strictly termed “state 2”, is functionally the same as state 4, and the latter term is usually used for both states''’ (Nicholls & Ferguson 1992).<br />
<br />
:::: Thus [[State 2]] was re-defined as functionally the same as [[State 4]]. State 2 (Chance and Williams 1955, 1956), however, is substrate-limited residual oxygen consumption at high ADP (ROX<sub>D</sub>), whereas ''L''<sub>N</sub> and ''L''<sub>T</sub> (State 4) are LEAK states in the absence of adenylates (''L''<sub>N</sub>: no ADP, no ATP) or presence of ATP (''L''<sub>T</sub>).<br />
<br />
:::: To overcome the termonological confusion persisting in the scientific literature, the respiratory coupling states of [[LEAK respiration]], [[OXPHOS capacity]] and [[ETS capacity]] are distinguished from residual oxygen consumption ([[ROX]]; [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger 2009]]).<br />
<br /><br />
<br />
<br />
=== b) LEAK state with oligomycin ===<br />
[[File:LEAK_Omy.jpg|left|300px|thumb|LEAK respiration induced by inhibition of ATP synthase by oligomycin, ''L''<sub>Omy</sub>.]] [[LEAK state with Omy|LEAK state with oligomycin]]: ''L''<sub>Omy</sub> (in isolated mitochondria or other [[mitochondrial preparations]], and intact cells).<br />
<br /><br />
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=== c) LEAK state without adenylates ===<br />
[[File:LEAK_N.jpg|left|300px|thumb|LEAK respiration without adenylates, ''L''<sub>N</sub>.]]<br />
:::: [[LEAK state without adenylates]]: ''L''<sub>N</sub> (in isolated mitochondria or other mitochondrial preparations, using a protocol different from the classical State 2-3-4 sequence).<br />
<br />
:::: Sequential addition of (1) mitochondria, (2) ADP, and (3) reduced substrates is the basis of the original [[State 1]]-[[State 2|2]]-[[State 3|3]] definitions of respiratory states (Chance and Williams 1955 part III, 1956), where [[State 2]] is zero respiration or residual oxygen consumption in the absence of substrate. An alternative protocol is well established, as shown e.g. by the classical Fig. 5A (Chance and Williams 1955 part I): 600 µM ADP is added after a state described as ‘Aerobic mitochondria plus succinate’. That state was never defined as ‘State 2’ by Brit Chance. Later Estabrook (1967) made this protocol more popular, with addition of substrate before any ADP or ATP was added.<br />
<br />
:::: In this alternative protocol, a respiratory LEAK state is induced in isolated mitochondria, permeabilized tissues, or permeabilized cells, adding the mitochondrial preparation to respiration medium containing inorganic phosphate ([[State 1]]), then adding reduced substrate (no external adenylates). This second state (Estabrook 1967) is a non-phosphorylating LEAK state, ''L''<sub>N</sub> (N for no adenylates; Gnaiger 2009), when substrate-saturated respiration compensates for the proton leak (mainly) in the absence of ADP.<br />
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<br />
== Related terms in MitoPedia ==<br />
=== State 4 versus State 2 ===<br />
::::* [[State 4]] - in contrast: [[State 2]]<br />
<br />
=== Static head ===<br />
::::* [[LEAK state]] - [[Static head]]<br />
:::: [[State 4]] is frequently referred to as 'static head' of isolated mitochondria. Equivalence requires testing, if at State 4 (in a protocol defined by Chance and Williams 1955) ATPase activity is actually zero, such that respiration at State 4 is not partially stimulated by partial recycling of ATP to ADP. In the latter case, State 4 respiration would be higher than [[LEAK respiration]] and thus higher than respiration at static head.<br />
<br />
[[Image:OXPHOS-coupled energy cycles.jpg|right|300px||link=Gnaiger 2012 MitoPathways|OXPHOS-coupled energy cycles. Source: The Blue Book]]<br />
=== Respiratory coupling states ===<br />
:::: [[File:P.jpg |link=OXPHOS capacity]] [[OXPHOS capacity |OXPHOS]], ''P''<br />
<br />
:::: [[File:R.jpg |link=ROUTINE respiration]] [[ROUTINE respiration |ROUTINE]], ''R''<br />
<br />
:::: [[File:E.jpg |link=ETS capacity]] [[ETS capacity |ETS]], ''E''<br />
<br />
:::: [[File:L.jpg |link=LEAK respiration]] [[LEAK respiration |LEAK]], ''L''<br />
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== References ==<br />
<br />
:::# Caplan SR, Essig A (1983) Bioenergetics and linear nonequilibrium thermodynamics. The steady state. Harvard Univ. Press, Cambridge:435 pp.<br />
:::# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217:383-93.<br />
:::# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217:409-27.<br />
:::# Chance B, Williams GR (1956) The respiratory chain and oxidative phosphorylation. Adv Enzymol 17:65-134.<br />
:::# Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Methods Enzymol 10:41-7.<br />
:::# Gnaiger E (1993a) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo:77-109. »[[Gnaiger_1993_Hypoxia |Bioblast Access]]«<br />
:::# Gnaiger E (1993b) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. »[[Gnaiger_1993_Pure Appl Chem |Open Access]]«<br />
:::# Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–45. »[[Gnaiger 2009 Int J Biochem Cell Biol| PMID: 19467914]]«<br />
:::# Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »[[Gnaiger 2014 MitoPathways |Open Access]]«<br />
:::# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3. Academic Press, London:287 pp.<br />
:::* [[Bioblast_alert_2014#Bioblast_alert_2014.2804.29:_2014-07-07|Bioblast alert 2014(04)]]<br />
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[[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]<br />
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<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» List of publications: LEAK'''</span><br />
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::: <span style="font-size:105%; color:##424242">'''» Abstracts: LEAK'''</span><br />
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{{#ask:[[Category:Abstracts]] [[Coupling states::LEAK]]<br />
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<br /></div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=Talk:High-resolution_respirometry&diff=135140Talk:High-resolution respirometry2017-05-04T06:25:43Z<p>Bitschnau Barbara: </p>
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<div>[[Image:BB-Bioblast.jpg|left|30px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]]<br />
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== Popular Bioblast page ==<br />
::: [[O2k high-resolution respirometry]] has been accessed more than <br />
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<hr />
<div>{{MitoPedia<br />
|abbr=D<br />
|description='''Adenosine diphosphate''' is a nucleotid. In [[OXPHOS]] core metabolism, ADP is a substrate of [[ANT]] and [[ATP synthase]] in the [[phosphorylation system]]. ADP is the discharged or low-energy counterpart of [[ATP]]. ADP can accept chemical energy by regaining a phosphate group to become ATP, in substrate-level phosphorylation (in anaerobic catabolism), at the expense of solar energy (in photosynthetic cells) or chemiosmotic energy (respiration in heterotrophic cells). ADP is added to [[mitochondrial preparations]] at kinetically saturating concentrations to induce the active state for evaluation of [[OXPHOS capacity]].<br />
|info=[[MiPNet03.02]], [[MiPNet09.12]]<br />
}}{{MitoPedia topics<br />
|mitopedia topic=Substrate and metabolite<br />
}}<br />
== Application in [[HRR]] ==<br />
<br />
:::: '''D: ADP''' (Adenosine 5'diphosphate potassium salt, C<sub>10</sub>H<sub>15</sub>N<sub>5</sub>O<sub>10</sub>P<sub>2</sub>K), <br />
::::Merck 117105-1GM ('''Calbiochem''') FW = 501.3; store at 4 °C. Recommended due to lower ATP impurities, we use 'ADP-Mg' in [[AT_Innsbruck_OROBOROS |OROBOROS Lab]].<br />
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:::: Alternative source: Sigma A 5285, 1 g, store at -20 °C; FW = 501.3.<br />
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:::: <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span><br />
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::: '''Preparation of 500 mM ADP stock solution''' (dissolved in H<sub>2</sub>O):<br />
<br />
::::# Weigh 501.3 mg of ADP = 1 mmol ADP<br />
::::# Add 1.2 ml H<sub>2</sub>O (ADP is not dissolved at this stage).<br />
::::# Neutralize with 5 M KOH (approx. 450 µl). ADP will dissolve after addition of KOH.<br />
::::# Check pH and adjust to 7 if necessary.<br />
::::# Adjust final volume to 2 ml and divide into 0.2 ml portions.<br />
::::# Store at -80 °C.<br />
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::: '''Preparation of 500 mM ADP stock solution with 300 mM free Mg<sup>2+</sup>''' (dissolved in H<sub>2</sub>O):<br />
<br />
:::: To keep free [Mg<sup>2+</sup>] constant during respiration measurement in [[MiR06]] or MiR05, mix ADP with MgCl<sub>2</sub> (0.6 mol MgCl<sub>2</sub>/mol ADP).<br />
<br />
:::: '''MgCl<sub>2</sub>''' (Scharlau MA0036: MgCl<sub>2</sub>.6H<sub>2</sub>O, FW = 203.3)<br />
<br />
::::# Weigh 501.3 mg of ADP = 1 mmol ADP.<br />
::::# Add 1.2 ml H<sub>2</sub>O (ADP is not dissolved at this stage).<br />
::::# Neutralize with 5 M KOH (approx. 450 µl). ADP will dissolve after addition of KOH.<br />
::::# Add 121,98 mg MgCl<sub>2</sub>. White precipitate will occur, which will dissolve during 1-2 min stirring on magnetic stirrer at RT.<br />
::::# Check pH and adjust to 7 if necessary.<br />
::::# Adjust final volume to 2 ml and divide into 0.2 ml portions.<br />
::::# Store at -80 °C.<br />
<br />
<br />
:::: '''Comment''': In some cases it was observed, that after defreezing of the 'ADP-Mg' a white precipitate has formed again. If this is the case we recommend to prepare the ADP stock solution and a MgCl<sub>2</sub> solution (0.6 mol MgCl<sub>2</sub>/mol ADP) seperately and perform a titration of both solutions in immediate succession.<br />
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::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]]<br />
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::::* Titration volume: 4-20 µl using a 25 µl syringe (2 ml O2k-chamber).<br />
::::* Final concentration: 1-5 mM.<br />
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== ADP dependence of respiration ==<br />
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The assumption of linearity (linear regression of oxygen concentration over time) is frequently not valid for various reasons other than [[oxygen kinetics]]. In classical ‘State 3’, ADP levels are ‘high’ ([[Chance_1955_JBC-III|Chance and Williams, 1955]]), but not necessarily saturating ([[MitoPedia: Respiratory states]]). Then, an ADP-dependent decline of respiration is observed immediately after titration of a sub-saturating concentration of ADP, which is obscured by any linear regression. This has caused in the past a tremendous underestimation of the apparent Km for ADP, perpetuated even today with the uncritical application of non-adequate software implementing the simple linearity approach only. For a critical approach to ADP kinetics, see [[Gnaiger_2000_Proc_Natl_Acad_Sci_USA|Gnaiger et al 2000]] and [[Gnaiger_2001_Respir_Physiol|Gnaiger 2001]].</div>Bitschnau Barbarahttps://wiki.oroboros.at/index.php?title=LEAK_respiration&diff=135137LEAK respiration2017-05-04T06:14:16Z<p>Bitschnau Barbara: </p>
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<div>{{MitoPedia<br />
|abbr=''L''<br />
|description=[[File:L.jpg]] '''LEAK respiration''' or LEAK oxygen flux, ''L'', compensating for [[proton leak]], [[proton slip]], cation cycling and [[electron leak]], is a dissipative component of respiration which is not available for performing biochemical work and thus related to heat production. LEAK respiration is measured in the LEAK state, in the presence of reducing substrate(s), but absence of ADP (theoretically, absence of inorganic phosphate presents an alternative), or after enzymatic inhibition of the [[phosphorylation system]]. The '''LEAK state''' is the non-phosphorylating resting state of intrinsic [[Uncoupler|uncoupled]] or [[Dyscoupled respiration|dyscoupled respiration]] when oxygen flux is maintained mainly to compensate for the proton leak at a high chemiosmotic potential, when ATP synthase is not active. In this non-phosphorylating resting state, the electrochemical proton gradient is increased to a maximum, exerting feedback control by depressing oxygen flux to a level determined mainly by the proton leak and the H<sup>+</sup>/O<sub>2</sub> ratio. In this state of maximum protonmotive force, LEAK respiration is higher than the LEAK component in state ''P'' ([[OXPHOS capacity]]). The conditions for measurement and expression of respiration vary ([[oxygen flux]] in state ''L'', ''J''<sub>O2''L''</sub> or [[oxygen flow]] in state ''L'', ''I''<sub>O2''L''</sub>). If these conditions are defined and remain consistent within a given context, then the simple symbol ''L'' for respiratory state can be used as a substitute for the more explicit expression for respiratory activity.<br />
» [[LEAK_respiration#LEAK respiration: concept-linked terminology of respiratory states |'''MiPNet article''']]<br />
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]]<br />
}}<br />
{{MitoPedia concepts<br />
|mitopedia concept=Respiratory state, Recommended<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=EAGLE<br />
}}<br />
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::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::::» [[ADP]]<br />
[[Dyscoupled respiration]] <br />
::::» [[Electron leak]]<br />
::::» [[ETS capacity]]<br />
::::» [[LEAK state]]<br />
::::» [[LEAK state with ATP]]<br />
::::» [[LEAK state with Omy|LEAK state with oligomycin]]<br />
::::» [[LEAK state without adenylates]]<br />
::::» [[OXPHOS capacity]]<br />
::::» [[Oxygen flow]]<br />
::::» [[Oxygen flux]] <br />
::::» [[Phosphorylation system]]<br />
::::» [[Proton leak]]<br />
::::» [[Proton slip]]<br />
::::» [[ROX]]<br />
::::» [[State 1]]<br />
::::» [[State 2]]<br />
::::» [[State 3]]<br />
::::» [[State 4]]<br />
::::» [[State 4|State 4o]]<br />
::::» [[Static head]]<br />
::::» [[Uncoupler]]<br />
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</div><br />
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__TOC__<br />
= LEAK respiration: concept-linked terminology of respiratory states =<br />
[[File:LEAK-NS.jpg|400px|thumb|LEAK respiration corresponds to resting, non-phosphorylating electron transfer with a shortcircuit of the proton cycle across the inner mt-membrane due to intrinsic uncoupling or dyscoupling. 2[H] indicates the reduced hydrogen equivalents of CHO substrates and electron transfer to oxygen. H<sup>+</sup><sub>out</sub> are protons pumped out of the matrix phase. Proton leaks (a property of the inner mt-membrane) dissipate energy of translocated protons, and proton slip prevents full translocation of protons across the inner mt-membrane (a property of the proton pumps). Measurement of LEAK respiration is possible in intact cells by inhibition of the phosphorylation system and in mt-preparations supported by an ETS-competent substrate state, exemplifed as the NS-pathway (CI<small>&</small>II-linked substrate supply). Modified after [[Gnaiger 2014 MitoPathways]].]]<br />
{{Publication<br />
|title=Gnaiger E (2014) LEAK respiration: concept-linked terminology of respiratory states. Mitochondr Physiol Network 2014-07-04.<br />
|info=[[Gnaiger 2014 MitoPathways]]<br />
|authors=OROBOROS<br />
|year=2014<br />
|journal=MiPNet<br />
|abstract=[[File:L.jpg]] Mitochondrial respiratory states have been defined originally by Chance and Williams (1955) as a sequence (from 1 to 5) of titrations and transitions in a respiratory protocol, including [[State 4]] as a '''[[LEAK state]]''' of respiration obtained after exhaustion of the added [[ADP]]. The second state ([[State 2]]) is induced by addition of 'high ADP'. Confusion persists in the current literature as to the meaning of State 2, which can be resolved by a transition from a specific protocol-linked to a generalized '''concept-linked terminology'''. <br />
|mipnetlab=AT Innsbruck Gnaiger E<br />
}}<br />
{{Labeling<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, ETS<br />
|instruments=Theory<br />
}}<br />
<br />
== The LEAK state ==<br />
:::: LEAK states of respiration are frequently called [[State 4]], [[State 4o]], basal state, or inactive state. Importantly, [[State 2]] is not a LEAK state according to the classical definition. <br />
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:::: Respiration compensating for the [[proton leak]] is the main component of LEAK respiration. If proton leak were the only component involved, it would make sense to simply write leak respiration for the compensatory oxygen flux. [[Proton slip]] and cation cycling, however, are also involved to a larger or smaller extent to stimulate LEAK respiration. The upper case 'LEAK', therefore, should make us aware that this is more accurately considered as an acronym, and cannot be taken as a definitive delineation of the stimulatory mechanism in the LEAK state of respiration.<br />
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::::* ''Note'': The distinction between LEAK and ETS capacity helps to clarify the important difference between physiologically uncoupled or pathologically dyscoupled respiration (LEAK) in contrast to noncoupled respiration ([[ETS capacity]]).<br />
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== Protocols for measurement of LEAK respiration ==<br />
:::: The LEAK state can be induced experimentally in various ways, which may yield idential estimates of LEAK respiration, or may show deviations that help to critically assess the proper protocol to be applied in specific cases:<br />
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=== a) LEAK state with ATP ===<br />
[[File:LEAK_T.jpg|left|300px|thumb|LEAK respiration in the presence of ATP, ''L''<sub>T</sub>, and absence of ATPase activity.]] [[LEAK state with ATP]]: ''L''<sub>T</sub> (classical [[State 4]] in isolated mitochondria) after phosphorylation of ADP to ATP is completed, or when a high concentration of ATP is added in the absence of ADP ([[Gnaiger 2000 Proc Natl Acad Sci U S A]]).<br />
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:::: In contradiction to the original definition of State 2 ([[ROX]]), yet with reference to Chance and Williams (1956), 'State 2' has later been used for describing this functionally different state of [[LEAK respiration]]:<br />
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:::‘''State 2: substrate added, respiration low due to lack of ADP. .. the controlled respiration prior to addition of ADP, which is strictly termed “state 2”, is functionally the same as state 4, and the latter term is usually used for both states''’ (Nicholls & Ferguson 1992).<br />
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:::: Thus [[State 2]] was re-defined as functionally the same as [[State 4]]. State 2 (Chance and Williams 1955, 1956), however, is substrate-limited residual oxygen consumption at high ADP (ROX<sub>D</sub>), whereas ''L''<sub>N</sub> and ''L''<sub>T</sub> (State 4) are LEAK states in the absence of adenylates (''L''<sub>N</sub>: no ADP, no ATP) or presence of ATP (''L''<sub>T</sub>).<br />
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:::: To overcome the termonological confusion persisting in the scientific literature, the respiratory coupling states of [[LEAK respiration]], [[OXPHOS capacity]] and [[ETS capacity]] are distinguished from residual oxygen consumption ([[ROX]]; [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger 2009]]).<br />
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=== b) LEAK state with oligomycin ===<br />
[[File:LEAK_Omy.jpg|left|300px|thumb|LEAK respiration induced by inhibition of ATP synthase by oligomycin, ''L''<sub>Omy</sub>.]] [[LEAK state with Omy|LEAK state with oligomycin]]: ''L''<sub>Omy</sub> (in isolated mitochondria or other [[mitochondrial preparations]], and intact cells).<br />
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=== c) LEAK state without adenylates ===<br />
[[File:LEAK_N.jpg|left|300px|thumb|LEAK respiration without adenylates, ''L''<sub>N</sub>.]]<br />
:::: [[LEAK state without adenylates]]: ''L''<sub>N</sub> (in isolated mitochondria or other mitochondrial preparations, using a protocol different from the classical State 2-3-4 sequence).<br />
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:::: Sequential addition of (1) mitochondria, (2) ADP, and (3) reduced substrates is the basis of the original [[State 1]]-[[State 2|2]]-[[State 3|3]] definitions of respiratory states (Chance and Williams 1955 part III, 1956), where [[State 2]] is zero respiration or residual oxygen consumption in the absence of substrate. An alternative protocol is well established, as shown e.g. by the classical Fig. 5A (Chance and Williams 1955 part I): 600 µM ADP is added after a state described as ‘Aerobic mitochondria plus succinate’. That state was never defined as ‘State 2’ by Brit Chance. Later Estabrook (1967) made this protocol more popular, with addition of substrate before any ADP or ATP was added.<br />
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:::: In this alternative protocol, a respiratory LEAK state is induced in isolated mitochondria, permeabilized tissues, or permeabilized cells, adding the mitochondrial preparation to respiration medium containing inorganic phosphate ([[State 1]]), then adding reduced substrate (no external adenylates). This second state (Estabrook 1967) is a non-phosphorylating LEAK state, ''L''<sub>N</sub> (N for no adenylates; Gnaiger 2009), when substrate-saturated respiration compensates for the proton leak (mainly) in the absence of ADP.<br />
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== Related terms in MitoPedia ==<br />
=== State 4 versus State 2 ===<br />
::::* [[State 4]] - in contrast: [[State 2]]<br />
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=== Static head ===<br />
::::* [[LEAK state]] - [[Static head]]<br />
:::: [[State 4]] is frequently referred to as 'static head' of isolated mitochondria. Equivalence requires testing, if at State 4 (in a protocol defined by Chance and Williams 1955) ATPase activity is actually zero, such that respiration at State 4 is not partially stimulated by partial recycling of ATP to ADP. In the latter case, State 4 respiration would be higher than [[LEAK respiration]] and thus higher than respiration at static head.<br />
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[[Image:OXPHOS-coupled energy cycles.jpg|right|300px||link=Gnaiger 2012 MitoPathways|OXPHOS-coupled energy cycles. Source: The Blue Book]]<br />
=== Respiratory coupling states ===<br />
:::: [[File:P.jpg |link=OXPHOS capacity]] [[OXPHOS capacity |OXPHOS]], ''P''<br />
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:::: [[File:R.jpg |link=ROUTINE respiration]] [[ROUTINE respiration |ROUTINE]], ''R''<br />
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:::: [[File:E.jpg |link=ETS capacity]] [[ETS capacity |ETS]], ''E''<br />
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:::: [[File:L.jpg |link=LEAK respiration]] [[LEAK respiration |LEAK]], ''L''<br />
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== References ==<br />
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:::# Caplan SR, Essig A (1983) Bioenergetics and linear nonequilibrium thermodynamics. The steady state. Harvard Univ. Press, Cambridge:435 pp.<br />
:::# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217:383-93.<br />
:::# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217:409-27.<br />
:::# Chance B, Williams GR (1956) The respiratory chain and oxidative phosphorylation. Adv Enzymol 17:65-134.<br />
:::# Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Methods Enzymol 10:41-7.<br />
:::# Gnaiger E (1993a) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo:77-109. »[[Gnaiger_1993_Hypoxia |Bioblast Access]]«<br />
:::# Gnaiger E (1993b) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. »[[Gnaiger_1993_Pure Appl Chem |Open Access]]«<br />
:::# Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–45. »[[Gnaiger 2009 Int J Biochem Cell Biol| PMID: 19467914]]«<br />
:::# Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »[[Gnaiger 2014 MitoPathways |Open Access]]«<br />
:::# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3. Academic Press, London:287 pp.<br />
:::* [[Bioblast_alert_2014#Bioblast_alert_2014.2804.29:_2014-07-07|Bioblast alert 2014(04)]]<br />
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::: <span style="font-size:105%; color:##424242">'''» List of publications: LEAK'''</span><br />
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::: <span style="font-size:105%; color:##424242">'''» Abstracts: LEAK'''</span><br />
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<br /></div>Bitschnau Barbara