2-Deoxyglucose

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Bioblasts - Richard Altmann and MiPArt by Odra Noel
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MitoPedia

2-Deoxyglucose

Description

2-Deoxyglucose, also known as 2-deoxy-D-glucose is a glucose derivative which has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase .

Abbreviation: 2-DG

Reference: Proton_flux, O2k-pH_ISE-Module

Application in HRR

2-DG: 2-deoxy-D-glucose C6H1205); Sigma D6134, 1 g or 5 g, store at 2-8 °C; FW = 164.16
Preparation of 2 M stock solution (dissolved in H2O):
  1. weigh 1g of 2-deoxy-D-glucose
  2. dissolve 3.0458 mL H2O in a 10 mL-glass beaker
»O2k manual titrations: MiPNet09.12 O2k-Titrations
  • Titration volume: 25 µL using a 50 µL syringe
  • Final conc. in 2 mL O2k-chamber: 25 mM






MitoPedia topics: Inhibitor