Andric 2019a MiP2019
The aim of this study was to define the transcription profiles of the molecular markers of mitochondrial biogenesis and fusion/architecture, as well as the markers of mtDNA copy numbers in the peripheral blood mononuclear cells (PBMCs) from war veterans with/without post-traumatic stress disorder (PTSD). Also, in order to define signaling molecules involved in changes of transcription profiles immortalized human males monocytes were exposed in vitro to hormonal markers of PTSD.
RQ-PCR-results showed that the transcription profiles of above mentioned markers were disturbed, with high individual variability within the groups. A significant increase in the expression of the PPARGC1A transcript was observed in a group of subjects who currently have PTSD, as well as in the subjects with “life-time" PTSD, compared to healthy controls. PPARGC1B, NRF2 and MFN2 transcripts increased only in PBMCs of “life-time"-PTSD, while the level of transcripts for other investigated genes and the ratio of markers of mtDNA copy numbers showed no significant difference between groups. The in vitro results showed parallelism in the transcription profile of molecular markers of mitochondrial biogenesis with results obtained using the PBMCs of the PTSD study.
It should be emphasized that all results should be considered as preliminary because the technical/time constraints did not allow the analysis of a larger number of PTSD subjects. However, the results are first findings in the field and can be used as a solid base for further extensive multidisciplinary research in order to clarify the molecular mechanisms for the prevention and treatment of trauma-induced pathological conditions.
Labels: MiParea: mt-Biogenesis;mt-density, mt-Structure;fission;fusion, mtDNA;mt-genetics Pathology: Other
Organism: Human Tissue;cell: Blood cells
Affiliations and support
- Markovic AZ(1)*, Milosevic MM(1)*, Radovic SM(1), Starovlah IM(1), Brkljacic J(2), Vojnovic Milutinovic D(2), Matic G(2), Kostic TS(1), Andric SA(1)
- *These authors contributed equally to this work.
- Lab Reproductive Endocrinology Signaling, Lab Chronobiology Aging, Centre Excellence CeRES, Fac Sciences, Univ Novi Sad, Novi Sad, Serbia
- Dept Biochemistry, Inst Biological Research "Siniša Stankovic", Univ Belgrade, Belgrade, Serbia. - – [email protected]
- This work was supported by the grant no. 173057 and grant no. 451-0302807 Centre of Excellence CeRES from the Ministry of Education, Science and Technological Development, Republic of Serbia, as well as grant no. 2130 from the Autonomous Province of Vojvodina.