Arandarcikaite 2019 ESCI2019

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Arandarcikaite O, Borutaitė V (2019) Analysis of mitochondrial functions in ischemic brain model.

Link: ESCI 2019

Arandarcikaite O, Borutaite V (2019)

Event: ESCI 2019 Coimbra PT

Ischemic stroke causes disability or mortality with limited therapeutic options. Ischemia leads to inhibition of mitochondrial respiration rate and opening of mitochondrial permeability transition pore (MPTP) and cell death processes initiation. Previously we reported that ischemia inhibits mitochondrial respiration, but ischemia- induced MPTP does not cause the release of cytochrome c. The inhibition of MPTP formation just partly protected against necrosis [1]. The brain are constituted from two types of cells neurons and glial. And different parts of brain are different composition of neurons and glial cells, for example in cerebellum there are common neurons, while in cortex there are just 20 % of neurons. The risk for stroke increases with aging. Mostly for the brain ischemic experiments are performed by using young adult animals. What for, we performed a comparative analysis of respiratory functions of mitochondria isolated from cortex and cerebellum of rats at various ages: neonatal (7 days), young adults (2-3 months), mature adults (7-10 months), and aged (24 months). We found that ischemia caused persistent inhibition of respiration of mitochondria isolated from cortex at all ages of animals. Ischemia induced inhibition of cerebellar mitochondrial respiration at the age of 7 days, 2-3 and 24 months, except group 7-10 months there were no statistically significant effect of ischemia.

Summarizing the data suggest that ischemia induced injuries to mitochondria respiration are related with age of the animal and region of the brain.


Bioblast editor: Plangger M O2k-Network Lab: LT Kaunas Borutaite V


Labels: MiParea: Respiration 

Stress:Ischemia-reperfusion  Organism: Rat  Tissue;cell: Nervous system 



HRR: Oxygraph-2k 

MitoEAGLEPublication 

Affiliations

Lithuanian Univ Health Science, Neuroscience Inst, Eiveniu 4, Kaunas, Lithuania. - [email protected]


References

  1. Arandarcikaite O, Jokubka R, Borutaite V (2015) Neuroprotective effects of nitric oxide donor NOC-18 against brain ischemia-induced mitochondrial damages: role of PKG and PKC. Neurosci Lett 586:65-70.