Avin 2021 Sci Rep
|Avin KG, Hughes MC, Chen NX, Srinivasan S, O'Neill KD, Evan AP, Bacallao RL, Schulte ML, Moorthi RN, Gisch DL, Perry CGR, Moe SM, O'Connell TM (2021) Skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease. Sci Rep 11:9788.|
Avin Keith G, Hughes Meghan C, Chen Neal X, Srinivasan Shruthi, O'Neill Kalisha D, Evan Andrew P, Bacallao Robert L, Schulte Michael L, Moorthi Ranjani N, Gisch Debora L, Perry Christopher G R, Moe Sharon M, O'Connell Thomas M (2021) Sci Rep
Abstract: Chronic kidney disease (CKD) leads to musculoskeletal impairments that are impacted by muscle metabolism. We tested the hypothesis that 10-weeks of voluntary wheel running can improve skeletal muscle mitochondria activity and function in a rat model of CKD. Groups included (n = 12-14/group): (1) normal littermates (NL); (2) CKD, and; (3) CKD-10 weeks of voluntary wheel running (CKD-W). At 35-weeks old the following assays were performed in the soleus and extensor digitorum longus (EDL): targeted metabolomics, mitochondrial respiration, and protein expression. Amino acid-related compounds were reduced in CKD muscle and not restored by physical activity. Mitochondrial respiration in the CKD soleus was increased compared to NL, but not impacted by physical activity. The EDL respiration was not different between NL and CKD, but increased in CKD-wheel rats compared to CKD and NL groups. Our results demonstrate that the soleus may be more susceptible to CKD-induced changes of mitochondrial complex content and respiration, while in the EDL, these alterations were in response the physiological load induced by mild physical activity. Future studies should focus on therapies to improve mitochondrial function in both types of muscle to determine if such treatments can improve the ability to adapt to physical activity in CKD.
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style Pathology: Other
Organism: Rat Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS Pathway: N, NS HRR: Oxygraph-2k