Azide

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MitoPedia

Azide

Description

Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).

Abbreviation: Azd

Reference: MiPNet03.02

Application in HRR

Azd: Azide (Sodium azide, NaN3); Sigma S2002, SDS 25 g, store commercial stock at RT; FW = 65.01.
Caution: Very toxic!


Preparation of 4 M storage and stock solution (dissolved in H2O) for 2-mL O2k-chamber:
  1. Weigh 260 mg of sodium azide.
  2. Dissolve in 1 mL H20.
  3. Divide into 0.5 mL portions.
  4. Store at -20 °C.
»O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): ≥50 µL using a 100 µL Hamilton syringe.
  • Final concentration: ≥100 mM.


Preparation of 2 M stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
  1. Take 400 µL of the 4 M stock solution.
  2. Add 400 µL H2O.
»O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (0.5-mL O2k-chamber): ≥25 µL using a 100 µL Hamilton syringe.
  • Final concentration: ≥100 mM.




MitoPedia topics: Inhibitor