Boushel 2011 Mitochondrion

From Bioblast
Publications in the MiPMap
Boushel RC, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11:303-7.

Β» PMID: 21147270

Boushel RC, Gnaiger Erich, Calbet Jose AL, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin Bengt (2011) Mitochondrion

Abstract: Across a wide range of species and body mass a close matching exists between maximal conductive oxygen delivery and mitochondrial respiratory rate. In this study we investigated in humans how closely in vivo maximal oxygen consumption (VO(2)max) is matched to muscle tissue-specific OXPHOS capacity (State 3) respiration. High-resolution respirometry was used to quantify mitochondrial respiration from the biopsies of arm and leg muscles while in vivo arm and leg VO(2) were determined by the Fick method during leg cycling and arm cranking. We hypothesized that muscle mitochondrial respiratory rate exceeds that of systemic oxygen delivery. OXPHOS capacity of the deltoid muscle (4.3Β±0.4 mmol O(2)kg(-1)min(-1)) was similar to the in vivo VO(2) during maximal arm cranking (4.7Β±0.5 mmol O(2)kg(-1)min(-1)) with 6 kg muscle. In contrast, the mitochondrial OXPHOS capacity of the quadriceps was 6.9Β±0.5 mmol O(2)kg(-1)min(-1), exceeding the in vivo leg VO(2)max (5.0Β±0.2mmolO(2)kg(-1)min(-1)) during leg cycling with 20 kg muscle (P<0.05). Thus, when half or more of the body muscle mass is engaged during exercise, muscle mitochondrial respiratory capacity surpasses in vivo VO(2)max. The findings reveal an excess capacity of muscle mitochondrial respiratory rate over O(2) delivery by the circulation in the cascade defining maximal oxidative rate in humans.

β€’ O2k-Network Lab: CA Vancouver Boushel RC, AT Innsbruck Gnaiger E, AT Innsbruck Oroboros, ES CN Las Palmas Calbet JAL

Project of the Copenhagen Muscle Research Centre (CMRC), Rigshospitalet, Copenhagen, Denmark (Prof. Dr. Bengt Saltin). Logo of the CMRC Greenland 2004 Expedition, designed by Crownprince Frederik of Denmark.

Greenland expedition CMRC: science and adventure

Β» Greenland Expedition CMRC


Cited by

Gnaiger 2020 BEC MitoPathways
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2.

Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style 

Stress:Ischemia-reperfusion  Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: OXPHOS  Pathway: NS  HRR: Oxygraph-2k 

BEC 2020.2 

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