Carnitine

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Bioblasts - Richard Altmann and MiPArt by Odra Noel
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MitoPedia

Carnitine

Description

Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.

Abbreviation: Car

Application in HRR

Car: Carnitine (L-Carnitine inner salt; C7H15NO3), Sigma-Aldrich: C0158, store at RT, CAS: 541-15-1, M = 161.2 g·mol-1


Preparation of 250 mM stock solution (dissolved in H2O)
  1. Weigh 100 mg of Carnitine.
  2. Add 2.48 mL H2O.
  3. Divide into 100 µL portions.
  4. Store frozen at -20°C.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 4 µL using a 10 µL Hamilton syringe.
  • Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe
  • Final concentration: 0.5 mM.


Feeding carnitine


MitoPedia topics: Substrate and metabolite