Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Drahota 2005 Cell Biochem Biophys

From Bioblast
Publications in the MiPMap
Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem Biophys 43:87-94.

Β» PMID: 16043886

Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Cell Biochem Biophys

Abstract: Cytochrome c oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period. β€’ Keywords: Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent

β€’ O2k-Network Lab: CZ Prague Houstek J

Labels: MiParea: Respiration 

Organism: Rat  Tissue;cell: Heart  Preparation: Isolated mitochondria, Oxidase;biochemical oxidation  Enzyme: Complex IV;cytochrome c oxidase 

Coupling state: OXPHOS 

HRR: Oxygraph-2k