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Dubinski 2018 Biochim Biophys Acta

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Publications in the MiPMap
Dubinski AF, Camasta R, Soule TGB, Reed BH, Glerum DM (2018) Consequences of cytochrome c oxidase assembly defects for the yeast stationary phase. Biochim Biophys Acta 1859:445-58.

Β» PMID: 29567354

Dubinski AF, Camasta R, Soule TGB, Reed BH, Glerum DM (2018) Biochim Biophys Acta

Abstract: The assembly of cytochrome c oxidase (COX) is essential for a functional mitochondrial respiratory chain, although the consequences of a loss of assembled COX at yeast stationary phase, an excellent model for terminally differentiated cells in humans, remain largely unexamined. In this study, we show that a wild-type respiratory competent yeast strain at stationary phase is characterized by a decreased oxidative capacity, as seen by a reduction in the amount of assembled COX and by a decrease in protein levels of several COX assembly factors. In contrast, loss of assembled COX results in the decreased abundance of many mitochondrial proteins at stationary phase, which is likely due to decreased membrane potential and changes in mitophagy. In addition to an altered mitochondrial proteome, COX assembly mutants display unexpected changes in markers of cellular oxidative stress at stationary phase. Our results suggest that mitochondria may not be a major source of reactive oxygen species at stationary phase in cells lacking an intact respiratory chain. β€’ Keywords: Cytochrome c oxidase (complex IV) assembly, Mitochondria, Oxidative stress, Stationary phase, Yeast β€’ Bioblast editor: Kandolf G


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