Gilliam 2013 Free Radic Biol Med
| Gilliam LA, Fisher-Wellman KH, Lin CT, Maples JM, Cathey BL, Neufer PD (2013) The anticancer agent doxorubicin disrupts mitochondrial energy metabolism and redox balance in skeletal muscle. Free Radic Biol Med 65:988-96. |
Gilliam LA, Fisher-Wellman KH, Lin CT, Maples JM, Cathey BL, Neufer PD (2013) Free Radic Biol Med
Abstract: The combined loss of muscle strength and constant fatigue are disabling symptoms for cancer patients undergoing chemotherapy. Doxorubicin, a standard chemotherapy drug used in the clinic, causes skeletal muscle dysfunction and premature fatigue along with an increase in reactive oxygen species (ROS). As mitochondria represent a primary source of oxidant generation in muscle, we hypothesized that doxorubicin could negatively affect mitochondria by inhibiting respiratory capacity, leading to an increase in H2O2-emitting potential. Here we demonstrate a biphasic response of skeletal muscle mitochondria to a single doxorubicin injection (20 mg/kg). Initially at 2 h doxorubicin inhibits both Complex I- and II-supported respiration and increases H2O2 emission, both of which are partially restored after 24 h. The relationship between oxygen consumption and membrane potential (ฮฮจ(mt)) is shifted to the right at 24 h, indicating elevated reducing pressure within the electron transfer-pathway (ET-pathway). Respiratory capacity is further decreased at a later time point (72 h) along with H2O2-emitting potential and an increased sensitivity to mitochondrial permeability transition pore (mtPTP) opening. These novel findings suggest a role for skeletal muscle mitochondria as a potential underlying cause of doxorubicin-induced muscle dysfunction. โข Keywords: Chemotherapy, ET-pathway, Metabolism, Mitochondria, PmFBs, ROS, Reactive oxygen species, Skeletal muscle, TPP, electron transport system, mPTP, mitochondrial permeability transition pore, permeabilized fiber bundles, reactive oxygen species, tetraphenylphosponium
โข O2k-Network Lab: US NC Greenville Neufer PD
Labels: MiParea: Respiration, mt-Membrane, Pharmacology;toxicology
Pathology: Cancer
Stress:Permeability transition
Organism: Rat
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: OXPHOS, ET
Pathway: N, S
HRR: Oxygraph-2k, TPP
