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Gupta 2022 PNAS Nexus

From Bioblast
Publications in the MiPMap
Gupta R, Rhee KY, Beagle SD, Chawla R, Perdomo N, Lockless SW, Lele PP (2022) Indole modulates cooperative protein-protein interactions in the flagellar motor. https://doi.org/10.1093/pnasnexus/pgac035

ยป PNAS Nexus 1:pgac035. PMID: 35719892 Open Access

Gupta Rachit,  Rhee Kathy Y,  Beagle Sarah D,  Chawla Ravi, Perdomo Nicolas,  Lockless Steve W,  Lele Pushkar P (2022) PNAS Nexus

Abstract: Indole is a major component of the bacterial exometabolome, and the mechanisms for its wide-ranging effects on bacterial physiology are biomedically significant, although they remain poorly understood. Here, we determined how indole modulates the functions of a widely conserved motility apparatus, the bacterial flagellum. Our experiments in Escherichia coli revealed that indole influences the rotation rates and reversals in the flagellum's direction of rotation via multiple mechanisms. At concentrations higher than 1 mM, indole decreased the membrane potential to dissipate the power available for the rotation of the motor that operates the flagellum. Below 1 mM, indole did not dissipate the membrane potential. Instead, experiments and modeling indicated that indole weakens cooperative protein interactions within the flagellar complexes to inhibit motility. The metabolite also induced reversals in the rotational direction of the motor to promote a weak chemotactic response, even when the chemotaxis response regulator, CheY, was lacking. Experiments further revealed that indole does not require the transporter Mtr to cross the membrane and influence motor functions. Based on these findings, we propose that indole modulates intra- and inter-protein interactions in the cell to influence several physiological functions. โ€ข Keywords: PMF, Chemoreceptors, Chemotaxis, Exometabolome, Switching โ€ข Bioblast editor: Plangger M


Labels: MiParea: Respiration, Pharmacology;toxicology 


Organism: Eubacteria 

Preparation: Intact cells 



HRR: Oxygraph-2k 

2022-11