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Hansen 2022 Magn Reson Med

From Bioblast
Publications in the MiPMap
Hansen K, Hansen ESS, Jespersen NRV, Bøtker HE, Pedersen M, Wang T, Laustsen C (2022) Hyperpolarized 13C MRI reveals large changes in pyruvate metabolism during digestion in snakes.

» Magn Reson Med 88:890-900. PMID: 35426467 Open Access

Hansen Kasper,  Hansen Esben Soevsoe S,  Jespersen Nichlas Riise V,  Boetker Hans Erik,  Pedersen Michael,  Wang Tobias, Laustsen Christoffer (2022) Magn Reson Med

Abstract: Hyperpolarized 13C MRI is a powerful technique to study dynamic metabolic processes in vivo; but it has predominantly been used in mammals, mostly humans, pigs, and rodents.

In the present study, we use this technique to characterize the metabolic fate of hyperpolarized [1-13 C]pyruvate in Burmese pythons (Python bivittatus), a large species of constricting snake that exhibits a four- to tenfold rise in metabolism and large growth of the visceral organs within 24-48h of ingestion of their large meals.

We demonstrate a fivefold elevation of the whole-body lactate-to-pyruvate ratio in digesting snakes, pointing to a large rise in lactate production from pyruvate. Consistent with the well-known metabolic stimulation of digestion, measurements of mitochondrial respiration in hepatocytes in vitro indicate a marked postprandial upregulation of mitochondrial respiration. We observed that a low SNR of the hyperpolarized 13C produced metabolites in the python, and this lack of signal was possibly due to the low metabolism of reptiles compared with mammals, preventing quantification of alanine and bicarbonate production with the experimental setup used in this study. Spatial quantification of the [1-13 C]lactate was only possible in postprandial snakes (with high metabolism), where a statistically significant difference between the heart and liver was observed.

We confirm the large postprandial rise in the wet mass of most visceral organs, except for the heart, and demonstrated that it is possible to image the [1-13 C]pyruvate uptake and intracellular conversion to [1-13 C]lactate in ectothermic animals. Keywords: MRI, [1-13C]Pyruvate, Metabolism, Postprandial, Python, Reptile Bioblast editor: Plangger M O2k-Network Lab: DK Aarhus Boetker HE

Labels: MiParea: Respiration, Instruments;methods 

Organism: Reptiles  Tissue;cell: Liver  Preparation: Isolated mitochondria 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k