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Huerta 2019 Aquat Toxicol

From Bioblast
Publications in the MiPMap
Huerta B, Chung-Davidson YW, Bussy U, Zhang Y, Bazil JN, Li W (2019) Sea lamprey cardiac mitochondrial bioenergetics after exposure to TFM and its metabolites. Aquat Toxicol 219:105380.

Β» PMID: 31855722

Huerta B, Chung-Davidson YW, Bussy U, Zhang Yizhu, Bazil JN, Li Weiming (2019) Aquat Toxicol

Abstract: Population control of invasive sea lamprey relies heavily on lampricide treatment of infested streams. The lampricide 3-trifluoromethyl-4-nitrophenol (TFM) is thought to impair mitochondrial ATP production through uncoupling oxidative phosphorylation. However, the effect of TFM on the entire electron transport chain (complexes I to V) in the mitochondria is not clear. In addition, TFM is reduced in phase I metabolism by sea lamprey at higher levels than in other fish species. The effects of these TFM reductive metabolites on mitochondria have not been explored. In this study, we sought to examine the effects of TFM and its reductive metabolite amino-TFM (TFMa) on cardiac mitochondrial oxygen consumption and membrane potential to delineate potential mechanisms for toxicity. To determine if molecules with similar structure also exhibit similar effects on mitochondria, we used 4-nitro-3-methylphenol (NMP) and its reductive metabolites 4-amino-3-methylphenol (NMPa) and 4-nitroso-3-methylphenol (NMPn) for comparisons. We found that mitochondrial bioenergetics was heavily affected with increasing concentrations of TFM, NMP, and NMPa when complexes I and II of the electron transport chain were examined, indicating that the toxic action of these compounds was exerted not only by uncoupling complex V, but also affecting complexes I and II.

Copyright Β© 2019 Elsevier B.V. All rights reserved. β€’ Keywords: Lampricides, Mitochondria toxicity, Reductive metabolism, Sea lamprey β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: US MI East Lansing Bazil JN

Labels: MiParea: Respiration, Pharmacology;toxicology 

Organism: Fishes  Tissue;cell: Heart  Preparation: Isolated mitochondria 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, ROX  HRR: Oxygraph-2k 

Labels, 2020-02