Kampa 2018 MiPschool Tromso C2

From Bioblast
Rafal Kampa
Cardioprotective flavoniods as a natural modulators of mitoBKCa channel.

Link: MitoEAGLE

Kampa RP, Kicinska A, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P (2018)

Event: MiPschool Tromso-Bergen 2018


Potassium channels such as KATP, BKCa or Kv1.3 have been found in the mitochondrial inner membranes (mtIM) of various cells. It is believed that potassium channels regulate mitochondrial membrane potential, matrix volume, respiration, and Ca2+ ion homeostasis. There are hypotheses that mitochondrial BKCa (mtBKCa) channels play an important role in ischemic preconditioning. It was shown that mitoBKCa channels are potential targets for some flavonoids in the anti-ischemic strategies.

Our pervious study showed functional properties of the mtBKCa channel in mitochondria of endothelial cells (EA.hy 926). Large conductance (270 pS), voltage dependence, a high open-state probability at positive potentials, sensitivity to Ca2+, NS1619 (a BKCa channel opener) and paxilline (BKCa channel inhibitor) indicate similarity to the mammalian BKCa channel. Previously, these channels were reported in glioma, skeletal muscle, brain and cardiac tissues.

In the current study, single channel activity of the mtBKCa channel was measured with patch-clamp technique of the mitoplasts isolated from EA.hy 926 endothelial cell line. We have shown data describing regulation of the mtBKCa channel by different cardioprotective flavonoids (luteolin, quercitin and cyanidin). Cellular respiration tests were performed outusing the oximeter.

β€’ Bioblast editor: Plangger M

Labels: MiParea: mt-Membrane, Pharmacology;toxicology 

Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell 

Enzyme: Inner mt-membrane transporter  Regulation: Ion;substrate transport 

Event: C2, Oral  Flavonoids 

Affiliations and support

Kampa R(1,2), Kicinska A(3), Kulawiak B(2), Jarmuszkiewicz W(3), Szewczyk A(2), Bednarczyk P(1)
  1. Dept Biophysics, Warsaw Univ Life Sciences
  2. Lab Intracellular Ion Channels, Nencki Inst Experimental Biol; Warsaw
  3. Lab Bioenergetics, Adam Mickiewicz Univ, Poznan; Poland
This study was supported by a grant 2016/21/B/NZ1/02769 from the National Science Centre, Poland.
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