Lanza 2011 J Magnetic Resonance Imaging
|Lanza IR, Bhagra S, Nair KS, Port JD (2011) Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy: A cross-validation with in vitro measurements. J Magnetic Resonance Imaging DOI: 10.1002/jmri.22733.|
Abstract: Purpose: To cross-validate skeletal muscle oxidative capacity measured by 31P-MR spectroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults.
Materials and Methods: Oxidative capacity in vivo was determined from PCr recovery kinetics following a 30-s maximal isometric knee extension. State 3 respiration was measured in isolated mitochondria using high-resolution respirometry. A second cohort of 10 individuals underwent two 31P-MRS testing sessions to assess the test–retest reproducibility of the method.
Results: Overall, the in vivo and in vitro methods were well-correlated (r = 0.66–0.72) and showed good agreement by Bland Altman plots. Excellent reproducibility was observed for the PCr recovery rate constant (CV = 4.6%; ICC = 0.85) and calculated oxidative capacity (CV = 3.4%; ICC = 0.83).
Conclusion: These results indicate that 31P-MRS corresponds well with gold-standard in vitro measurements and is highly reproducible. • Keywords: phosphocreatine recovery;skeletal muscle;high-resolution respirometry;mitochondria;oxidative capacity
• O2k-Network Lab: US MN Rochester Nair KS
Organism: Human Tissue;cell: Skeletal muscle Preparation: Isolated mitochondria
Coupling state: OXPHOS