Lark 2016 Am J Physiol Cell Physiol

Lark DS, Torres MJ, Lin CT, Ryan TE, Anderson EJ, Neufer PD (2016) Direct real-time quantification of mitochondrial oxidative phosphorylation efficiency in permeabilized skeletal muscle myofibers. Am J Physiol Cell Physiol 311:C239-45.

» PMID: 27335172 Open Access

Lark DS, Torres MJ, Lin CT, Ryan TE, Anderson EJ, Neufer PD (2016) Am J Physiol Cell Physiol

Abstract: Oxidative phosphorylation (OXPHOS) efficiency, defined as the ATP-to-O ratio, is a critical feature of mitochondrial function that has been implicated in health, aging, and disease. To date, however, the methods to measure ATP/O have primarily relied on indirect approaches or entail parallel rather than simultaneous determination of ATP synthesis and O₂ consumption rates. The purpose of this project was to develop and validate an approach to determine the ATP/O ratio in permeabilized fiber bundles (PmFBs) from simultaneous measures of ATP synthesis (J_ATP) and O₂ consumption (J_O2) rates in real time using a custom-designed apparatus. J_O2 was measured via a polarigraphic oxygen sensor and J_ATP via fluorescence using an enzyme-linked assay system (hexokinase II, glucose-6-phosphate dehydrogenase) linked to NADPH production. Within the dynamic linear range of the assay system, ADP-stimulated increases in steady-state J_ATP mirrored increases in steady-state J_O2 (r(2) = 0.91, P < 0.0001, n = 57 data points). ATP/O ratio was less than one under low rates of respiration (15 μM ADP) but increased to more than two at moderate (200 μM ADP) and maximal (2,000 μM ADP) rates of respiration with an interassay coefficient of variation of 24.03, 16.72, and 11.99%, respectively. Absolute and relative (to mechanistic) ATP/O ratios were lower in PmFBs (2.09 ± 0.251, 84%) compared with isolated mitochondria (2.44 ± 0.124, 98%). ATP/O ratios in PmFBs were not affected by the activity of adenylate kinase or creatine kinase. These findings validate an enzyme-linked respiratory clamp system for measuring OXPHOS efficiency in PmFBs and provide evidence that OXPHOS efficiency increases as energy demand increases.

Copyright © 2016 the American Physiological Society. • Keywords: Adenosine 5′-triphosphate synthesis, Adenosine 5′-triphosphate-to-oxygen ratio, Oxidative efficiency, Permeabilized myofiber, Buffer Z

• O2k-Network Lab: US NC Greenville Neufer PD, US NC Greenville Anderson EJ, US TN Nashville Wasserman DH

Cited by

• Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001

Labels: MiParea: Respiration, Instruments;methods 

Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue, Isolated mitochondria 

Regulation: ADP, ATP production  Coupling state: LEAK, OXPHOS, ET  Pathway: N, Gp, NS  HRR: Oxygraph-2k 

MitoFit 2021 MgG