Mendelev 2011 Mitochondrion

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Mendelev N, Mehta SL, Witherspoon S, He Q, Sexton JZ, Li PA (2011) Upregulation of human selenoprotein H in murine hippocampal neuronal cells promotes mitochondrial biogenesis and functional performance. Mitochondrion 11:76-82.

» PMID: 20656065 Open Access

Mendelev N, Mehta SL, Witherspoon S, He Q, Sexton JZ, Li PA (2011) Mitochondrion

Abstract: Overexpression of selenoprotein H (SelH) gene provides neuroprotection in neurons against UVB-induced cell death by blocking the mitochondrial-initiated apoptotic cell death pathway. This study examined the effects of SelH on mitochondrial biogenesis and mitochondrial function. The results demonstrated that overexpression of SelH gene in neuronal HT22 cells significantly increased the levels of mitochondrial biogenesis regulators, nuclear respiratory factor-1 (NRF-1), peroxisome proliferator-activated receptor-γ coactivator-1 alpha (PGC-1α) and mitochondrial transcription factor A (Tfam). Mitochondrial cytochrome c content was elevated, mass was increased and respiration was enhanced. SelH transfection ameliorated ultra violet B (UVB)-induced suppression of mitochondrial biogenesis markers and depolarization of mitochondrial membrane potential. Overexpression of SelH promotes mitochondrial biogenesis and improves mitochondrial functional performance.

Keywords: Mitochondrion, Mitochondrial biogenesis, Mitochondrial respiration, Neuron, Selenoprotein, cell signaling, HT22 cells

O2k-Network Lab: US NC Durham Li PA


Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Genetic knockout;overexpression, mt-Medicine 

Stress:Oxidative stress;RONS  Organism: Mouse  Tissue;cell: Nervous system  Preparation: Intact cells 


Coupling state: OXPHOS 

HRR: Oxygraph-2k