Meza-Buendia 2022 PLoS One

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Publications in the MiPMap
Meza-Buendia AK, Aparicio-Trejo OE, Díaz F, Caamal-Monsreal C, Pedraza-Chaverri J, Álvarez-Delgado C, Paschke K, Rosas C (2022) High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart.

Β» PLoS One 17:e0273554. PMID: 36037204 Open Access

Meza-Buendia Ana Karen, Aparicio-Trejo Omar Emiliano, Diaz Fernando, Caamal-Monsreal Claudia, Pedraza-Chaverri Jose, Alvarez-Delgado Carolina, Paschke Kurt, Rosas Carlos (2022) PLoS One

Abstract: Mitochondrial respirometry is key to understand how environmental factors model energetic cellular process. In the case of ectotherms, thermal tolerance has been hypothesized to be intimately linked with mitochondria capability to produce enough adenosine triphosphate (ATP) to respond to the energetic demands of animals in high temperatures. In a recent study made in Octopus maya was proposed the hypothesis postulating that high temperatures could restrain female reproduction due to the limited capacity of the animals' heart to sustain oxygen flow to the body, affecting in this manner energy production in the rest of the organs, including the ovarium Meza-Buendia AK et al. (2021). Unfortunately, until now, no reports have shown temperature effects and other environmental variables on cephalopod mitochondria activity because of the lack of a method to evaluate mitochondrial respiratory parameters in those species' groups. In this sense and for the first time, this study developed a method to obtain mitochondrial respirometry data of adult Octopus maya's heart. This protocol illustrates a step-by-step procedure to get high yield and functional mitochondria of cephalopod heart and procedure for determining the corresponding respiratory parameters. The procedure described in this paper takes approximately 3 to 4 hours from isolation of intact mitochondria to measurement of mitochondrial oxygen consumption.

β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: MX Mexico City Pedraza Chaverri J

Labels: MiParea: Respiration, Instruments;methods 

Organism: Molluscs  Tissue;cell: Heart  Preparation: Isolated mitochondria 

Regulation: Temperature  Coupling state: LEAK, OXPHOS  Pathway: ROX  HRR: Oxygraph-2k 


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