MiRK03

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Bioblasts - Richard Altmann and MiPArt by Odra Noel
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MitoPedia

MiRK03

Description

Mitochondrial respiration medium, MiRK03, modified after a medium described by Komary 2010 Biochim Biophys Acta, intended for use as medium for H2O2 production measurement with Amplex Red.

Abbreviation: MiRK03

Reference: Krumschnabel 2014 Methods Mol Biol, Komary 2010 Biochim Biophys Acta






MitoPedia topics: Media for respirometry 

MiRK03

substance MiRK03 molecular weight for 1 l
EGTA 0.5 mM 380.35 g/mol 190.18 mg
MgCl2 x 6 H2O 3 mM 203.30 g/mol 609.90 mg
HEPES 20 mM 238.31 g/mol 4 766.2 mg
KCl 130 mM 74.56 g/mol 9 692.8 mg
KH2PO4 10 mM 136.09 g/mol 1 360.9 mg
BSA 1 mg/ml - 1000 mg

Preparation without BSA

  1. Weigh all ingredients (besides BSA) according to the table above and transfer them into a big glass beaker.
  2. Add about 750 mL H2O.
  3. Under stirring adjust the temperature to 25 °C.
  4. Adjust the pH to 7.12 with 5 M KOH.
  5. Adjust final volume to 1 L and divide in 40 mL portions.
  6. Store at -20 °C.

MiRK03 ph - temperature dependence

data recorded with MiRK03 without BSA (only averaged values are shown; two measurements were conducted)

temperature [°C] pH of MiRK03
20 7.15
25 7.12
30 7.09
35 7.05
37 7.03