Mortensen 2015 Adv Exp Med Biol
|Mortensen OH, Jørgensen W, Frandsen L, Grunnet N, Quistorff B (2015) Effects of a high fat diet and taurine supplementation on metabolic parameters and skeletal muscle mitochondrial function in rats. Adv Exp Med Biol 803:387-95.|
Abstract: Obesity and consumption of a high fat diet are risk factors for the metabolic syndrome and is thought to confer changes in skeletal muscle mitochondrial function. Taurine supplementation has been shown to be able to counteract at least part of the metabolic changes induced by consumption of a high fat diet, yet little is known about the effect of taurine supplementation upon mitochondrial function. Here we assessed the effect of taurine supplementation on glucose and lipid parameters as well as skeletal muscle mitochondrial function in a high fat diet rat model.
Male Wistar rats were fed either a control diet, a high fat diet or a high fat diet with taurine supplementation (2 % in the drinking water) for 12 weeks.
High fat diet caused an increase in body weight and a marked glucose intolerance. Taurine had no effect on body weight or glucose tolerance. We saw no difference between groups with regard to fasting plasma glucose, free fatty acids or triglycerides or skeletal muscle triglyceride content. However, high fat diet resulted in a marked increase in hepatic triglyceride content, which was counteracted by taurine. High fat diet increased liver, but not skeletal muscle or plasma taurine concentration. Taurine caused an increase in plasma, liver and skeletal muscle taurine concentration. High fat diet increased state 3 respiration in skeletal muscle when using pyruvate as substrate, with no effect of taurine.
In conclusion, taurine counteracted a subset of parameters changed by the high fat diet, but had no effect on mitochondrial function.
• O2k-Network Lab: DK Copenhagen Quistorff B
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Rat Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Coupling state: OXPHOS Pathway: N HRR: Oxygraph-2k