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NextGen-O2k Documentation

From Bioblast

Bioblast 2022



NextGen-O2k Documentation

NextGen-O2k

The revolutionary all-in-one instrument to conquer mitochondrial disease.


WP3: Documentation



Abstract

In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.

Achievements

Project Year 1: In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes.
Project Year 2: Year 2 proved to be a continuation of the development of SOPs and video clips. A PB-Module manual was released for the algae KOLs and new video clips ranged from basic set-up and quality control to more advanced techniques like the DIC saturation curve using the PB Module. These materials provide step-by-step instructions, support and information for using the NextGen-O2k. Furthermore, trailers for the new modules (Q, NADH and PB) were recorded and published.

Progress and next steps

See » Track record

PlayVideo.jpg NextGen-O2k Videosupport clips

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NextGen-O2k demo

  • NextGen-O2k demo: Coenzyme Q redox state and mt-respiration
  • NextGen-O2k demo: mt-respiration and NADH
  • NextGen-O2k demo: mt-respiration and H2O2
  • NextGen-O2k demo: H2O2 calibration



O2k-Manual.jpg NextGen-O2k Manuals

» NextGen-O2k manual
» DatLab8.0: CV-Module
» NextGen-O2k: Q-Module
» NextGen-O2k: NADH-Module
» NextGen-O2k: PB-Module
» O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8
» Oxygen flux_analysis - DatLab 7.4
» Safranin Analysis Template
» General template for mt-membrane potential analysis
» H2O2 flux analysis