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NextGen-O2k Scientific developments

From Bioblast

Bioblast 2022



NextGen-O2k Scientific developments

NextGen-O2k

The revolutionary all-in-one instrument to conquer mitochondrial disease.


WP2: Scientific developments



Abstract

The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
  • Determination of optimal Q-sensor measurement conditions
  • Determination of optimal measurement conditions for the PB-Module
  • Development of protocols for scientific, pre-clinical and algal biotechnology applications
  • Data handling and quality control

Achievements

Project Year 1: During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures.
Project Year 2: During the second project year, measurement conditions and protocols were optimized for the PB- and Q-Modules with a variety of biological samples. Additionally, the NADH-Module for evaluation of the NADH-pool in physio-pathological conditions was integrated in the NextGen-O2k. Testing of modules for the DatLab8 software were conducted and improvements applied. Moreover, SUIT protocols for general, pre-clinical, and algal biotechnology applications were developed. Quality control was maintained during the second period of project, an important factor in the development of the NexGen-O2k instrument and relevant protocols.

Progress and next steps

See » Track record

NextGen-O2k MitoFit Preprints

LinkViewReferenceYear
MitoFit Preprints 2023.2.
MitoFit pdf
Functional hypoxia reduces mitochondrial calcium uptake.
Donnelly C, Komlódi T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-00022023
MitoFit Preprints 2022.01.v3.
MitoFit pdf
Proline oxidation leading to high electron flow through reduction of ubiquinone supports ATP production by F1FO-ATPase in mitochondria with inhibited Complex I
Pallag G, Nazarian S, Ravasz D, Bui D, Komlódi T, Doerrier C, Gnaiger E, Seyfried TN, Chinopoulos C (2022) Proline oxidation leading to high electron flow through reduction of ubiquinone supports ATP production by F1FO-ATPase in mitochondria with inhibited Complex I. https://doi.org/10.26124/mitofit:2022-0001.v32022-05-04 published in https://doi.org/10.3390/ijms230951112022
MitoFit Preprints 2021.05.
MitoFit pdf


Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-00052021
MitoFit Preprint Arch EA20.1.
MitoFit pdf
High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations
Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations. https://doi.org/10.26124/mitofit:ea20.algaeurope.00012020