Oliveira 2011 Abstract IOC65
Oliveira MP, Goncalves RLS, Oliveira MF (2011) Studies on the mitochondrial metabolism in the blood fluke Schistosoma mansoni. MiPNet16.03. |
Link:
Oliveira MP, Goncalves RLS, Oliveira MF (2011)
Event: IOC65
Mitochondria perform multiple roles in cell biology and is the main site of aerobic ATP production and reactive oxygen species generation. The blood fluke Schistosoma mansoni is the ethiological agent of human schistosomiasis that rely on vertebrate blood to meet their energy demands during their vertebrate stages. The worm has a complex life-cycle, in such a way that adult parasite stages reply more on the fermentative metabolism. Here, we assessed mitochondrial function of adult stages of S. mansoni: the adult males (AM) and females (AF). Oxygen consumption analyses on intact parasites were carried out polarographically at 37oC. Using a Hankβs balanced salt solution containing 5,5 mM glucose, we observed that fresh AF and AM consumed 0.28 and 0.85 pmols oxygen/s/worm, respectively. In AF, antimycin a (AA) inhibited oxygen consumption in 59%, while in AM, AA caused 90% inhibition of respiration. Oligomycin caused 50% inhibition of oxygen comsumption in AF, compared to 67% in AM. Also, the mitochondrial and non-mitochondrial O2 consumption was, respectively, 68% and 32% in AF and 88% and 12% in AM. The O2 consumption due to oxidative phosphorylation in AF was 52.54% in AF while in AM was 58.71%. The content of functional F1FO ATP synthase in AF was 4.93 Β΅g/mL and 9.63Β΅g/mL in AM. Citrate synthase and cytochrome c oxidase activities were significantly higher in AM compared to AF. Finally, glucose uptake in freshly isolated parasites was significantly higher in AF compared to AM. Collectively, these data indicate that the energy metabolism in S. mansoni is strikingly distinct among sexes in such a way that AM are more dependent on mitochondrial function than AF.
Support: CAPES, FAPERJ, ICGEB, CNPq.
β’ Keywords: Schistosoma mansoni
β’ O2k-Network Lab: BR Rio de Janeiro Oliveira MF
Labels:
Stress:Oxidative stress;RONS Organism: Other invertebrates
HRR: Oxygraph-2k