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Oliveira Pinto 2013 Abstract IOC75

From Bioblast
Oliveira Pinto M (2013) Studies on the energy and redox metabolism of the blood fluke Schistosoma mansoni. Mitochondr Physiol Network 18.03.

Link: IOC75 Open Access

Oliveira Pinto M, Oliveira MF (2013)

Event: IOC75

The blood fluke Schistosoma mansoni is an intravascular parasite which causes human schistosomiasis. Within the human host, the adult forms of S. mansoni depend on the ingestion of large amounts of blood to supply their energy demands. Furthermore, evidence indicates that important metabolic changes occur in worms in several stages of their life cycle, so that the cercariae present essentially an oxidative metabolism, while the adult forms living within the vertebrate blood vessels have a more fermentative metabolism. Furthermore, the activity and expression of antioxidant enzymes increases during development of the worm in the vertebrate host which is parallel with the ingestion of blood. Thus, we aim to investigate the redox and energy metabolism in adult forms of S. mansoni. We observed that the oxygen consumption associated with the electron transfer-pathway (ET-pathway) is significantly lower in females than in males. This phenomenon occurs independently of the type of substrate used by worms. Glucose uptake was higher in females than in males suggesting increased dependence on glucose metabolism in females. Evaluation of ET-pathway complex activities showed that females have higher Complex I-III and lower Complex IV compared to males. Hydrogen peroxide production was significantly higher in whole females than in males. Pro-oxidant challenge with menadione shoed that females are significant more resistant than males. We therefore conclude that there is a difference in energy metabolism and redox between adult female and adult male worms, which may be relevant to allow these organisms to the blood feeding habit.

β€’ Keywords: Schistosoma mansoni, metabolism, redox resistance

β€’ O2k-Network Lab: BR_Rio de Janeiro_Oliveira MF

Labels: MiParea: Respiration, Comparative MiP;environmental MiP, Gender, Developmental biology, mt-Medicine  Pathology: Other  Stress:Oxidative stress;RONS  Organism: Other invertebrates 

Preparation: Intact organism 

Regulation: Aerobic glycolysis, Redox state  Coupling state: OXPHOS  Pathway: Other combinations  HRR: Oxygraph-2k 

Affiliations and author contributions

Institute of Medical Biochemistry, Federal University of Rio de Janeiro