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Q-Module
Description
The Q-Module, developed both for measuring the Q redox state and for cyclic voltammetry measurements, is an integral part of the NextGen-O2k and consists of the Q-Sensor, integrated electronic components in the O2k, and the DatLab software.
Abbreviation: Q-Module
The Q-Module is part of the NextGen-O2k project
In the NextGen-O2k project we are working on developing the Q-Module for the new series of our O2k-FluoRespirometer, the NextGen-O2k. The Q-Module allows us to monitor the Q redox state of the Q-junction using the specific Q-Stoppers with the integrated three-electrode system and the modified electronics inside the O2k housing.
Communicated by Komlodi T, Cardoso LHD 2020-07-28
Q-Module Manual
The Q-Module manual can be found here: MiPNet24.12 NextGen-O2k: Q-Module
MitoPedia: NextGen-O2k
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Term | Abbreviation | Description |
---|---|---|
Hydrogen peroxide | H2O2 | Hydrogen peroxide, H2O2 or dihydrogen dioxide, is one of several reactive oxygen intermediates generally referred to as reactive oxygen species (ROS). It is formed in various enzyme-catalyzed reactions (e.g., superoxide dismutase) with the potential to damage cellular molecules and structures. H2O2 is dismutated by catalase to water and oxygen. H2O2 is produced as a signaling molecule in aerobic metabolism and passes membranes more easily compared to other ROS. |
Mitochondrial membrane potential | mtMP, Δψ [V] | The mitochondrial membrane potential, mtMP, is the electric part of the protonmotive force, ΔpH+.
Δψ = ΔpH+ - ΔµH+ / F mtMP or Δψ is the potential difference across the inner mitochondrial (mt) membrane, expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per ‘motive’ electron or per electron moved across the transmembrane potential difference, with the number of ‘motive’ electrons expressed in the unit coulomb [C]. |
NextGen-O2k | ||
NextGen-O2k Instrument | NextGen-O2k Instrument | |
NextGen-O2k Technical developments | ||
Oxygen kinetics | Oxygen kinetics describes the dependence of respiration of isolated mitochondria or cells on oxygen partial pressure. Frequently, a strictly hyperbolic kinetics is observed, with two parameters, the oxygen pressure at half-maximum flux, p50, and maximum flux, Jmax. The p50 is in the range of 0.2 to 0.8 kPa for cytochrome c oxidase, isolated mitochondria and small cells, strongly dependent on Jmax and coupling state. | |
PB-Module | PB-Module | The PB-Module has been developed for conducting measurements of PhotoBiology, including photosynthesis. It consists of the PB-Sensor and electronic components which are an integral part of the NextGen-O2k. Measurements are recorded and evaluated with the DatLab 8 software. |
PB-Sensor | The PB-Sensor has been designed as a part of the PB-Module to provide with an external source of light, in the range of the blue (451 nm) or red (634 nm) wavelengths. This enables experiments for evaluating the production/consumption of O2 in the presence of light. The PB-Sensor consists on one LED and one photodiode mounted on the sensor tip behind a plastic cover. The LED emits with blue or red light on the sample and its intensity can be regulated from 0 to 5000 µEinsteins*m-2*s-1. The photodiode provides real-time measurement of the light intensity allowing for continuous adjustment to the desired value, which can also help compensate for the temperature rise due to the operation of the LED. | |
Q-Module | Q-Module | The Q-Module, developed both for measuring the Q redox state and for cyclic voltammetry measurements, is an integral part of the NextGen-O2k and consists of the Q-Sensor, integrated electronic components in the O2k, and the DatLab software. |
Q-Sensor | The Q-Sensor has been designed as a part of the Q-Module for measurements with cyclic voltammetry and voltammetry, allowing for analysis of the Q redox state. The Q-Stopper with the reference electrode is called Q-Sensor, which is plugged in the NextGen-O2k. A three-electrode system is used to detect the Q redox state. Two of the three electrodes (glassy carbon and platinum electrode) are built into the Q-Stopper, while the reference electrode is removable (Reference-Electrode\2.4 mm). |
- Bioblast links: Q - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coenzyme Q
- » Coenzyme Q
- » Coenzyme Q2
- » Ubiquinone
- » Semiquinone
- » Quinol
- » Quinone
- » Q redox state
- » Q-cycle
- Coenzyme Q
- Mitochondrial pathways, respiratory Complexes, and Q
- » Q-junction
- » Convergent electron flow
- » NS-pathway
- » FNS
- » FNSGp
- Mitochondrial pathways, respiratory Complexes, and Q
- NextGen-O2k and Q-Module
- Bioblast links: NADH - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Mitochondrial NADH and respiration
Mitochondrial NADH production
NextGen-O2k
- Bioblast links: Hydrogen peroxide - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- General
- MiPNet O2k-Procedures
- Bioblast links: ATP production - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Phosphorylation pathway
Phosphorylation pathway substrates
- » ADP
- » ATP
- » Inorganic phosphate
Phosphorylation pathway inhibitors
Coupling control
Respiratory complexes and coupling
ATP production measurement
- Bioblast links: pH and protons - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- pH and protons
- » pH
- » hydrogen ion
- » proton
- » pH buffering capacity
- » proton flux
- » proton pump versus hydrogen ion pump
- » proton leak
- » proton slip
- » protonmotive force
- pH and protons
- O2k-pH
- » O2k-Catalogue: O2k-pH ISE-Module
- » O2k-Manual pH electrode: MiPNet23.15 O2k-pH ISE-Module
- » O2k-SOP: MiPNet08.16 pH calibration
- » File:PH-Calibration-List.xls
- » NextGen-O2k, ratiometric: Carboxy SNARF 1
- » NextGen-O2k, ratiometric: HPTS
- » pH calibration buffers
- O2k-pH
- O2k-Publications
- HRFR - general
- » O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
- » O2k signals and output
- » O2k-SOP: MiPNet14.06 Instrumental O2 background
- » MiPNet19.18A O2k-Series G: Start
- » ESD
- » O2k configuration
- » O2k control
- » O2k-FluoRespirometer
- » O2k-Main Unit#O2k-Series
- » Titration-Injection microPump
- » Compare: O2k-TPP+_ISE-Module
- HRFR - general
- Bioblast links: Calcium - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Inhibitors
- General
- Bioblast links: PhotoBiology and plant physiology - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
PhotoBiology: photosynthesis
Plant physiology: respiration
NextGen-O2k and PB-Module
- » NextGen-O2k
- » PB-Module
- » PB-Sensor
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MitoPedia:NextGen-O2k