Saponin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for permeabilization of muscle fibres.
Reference: Doerrier 2018 Methods Mol Biol
MitoPedia topics: Permeabilization agent
Application in HRR
Sap: Saponin (from quillaja bark); Sigma S 7900, 25 g, store at RT.
Comment: Saponin from Quillaja Bark has the product numbers S 7900 and S 2149, but S 2149 is withdrawn from Sigma.
Caution: Prepare fresh everyday!
Preparation of 5 mg/mL stock solution (dissolved in BIOPS):
- 1) Weigh 5 mg of saponin.
- 2) Dissolve in 1 mL BIOPS.
→ Use 50 µg saponin/mL BIOPS: add 20 µL of 5 mg/mL saponin stock solution per 2 mL BIOPS.