Sarabhai 2021 Diabetologia

From Bioblast
Publications in the MiPMap
Sarabhai T, Koliaki C, Mastrototaro L, Kahl S, Pesta D, Apostolopoulou M, Wolkersdorfer M, BΓΆnner AC, Bobrov P, Markgraf DF, Herder C, Roden M (2021) Dietary palmitate and oleate differently modulate insulin sensitivity in human skeletal muscle. Diabetologia 65:301-14.

Β» PMID: 34704121 Open Access

Sarabhai Theresia, Koliaki Chrysi, Mastrototaro Lucia, Kahl Sabine, Pesta Dominik, Apostolopoulou Maria, Wolkersdorfer Martin, Boenner Anna C, Bobrov Pavel, Markgraf Daniel F, Herder Christian, Roden Michael (2021) Diabetologia

Abstract: Energy-dense nutrition generally induces insulin resistance, but dietary composition may differently affect glucose metabolism. This study investigated initial effects of monounsaturated vs saturated lipid meals on basal and insulin-stimulated myocellular glucose metabolism and insulin signalling.

In a randomised crossover study, 16 lean metabolically healthy volunteers received single meals containing safflower oil (SAF), palm oil (PAL) or vehicle (VCL). Whole-body glucose metabolism was assessed from glucose disposal (Rd) before and during hyperinsulinaemic-euglycaemic clamps with D-[6,6-2H2]glucose. In serial skeletal muscle biopsies, subcellular lipid metabolites and insulin signalling were measured before and after meals.

SAF and PAL raised plasma oleate, but only PAL significantly increased plasma palmitate concentrations. SAF and PAL increased myocellular diacylglycerol and activated protein kinase C (PKC) isoform ΞΈ (p < 0.05) but only PAL activated PKCΙ›. Moreover, PAL led to increased myocellular ceramides along with stimulated PKCΞΆ translocation (p < 0.05 vs SAF). During clamp, SAF and PAL both decreased insulin-stimulated Rd (p < 0.05 vs VCL), but non-oxidative glucose disposal was lower after PAL compared with SAF (p < 0.05). Muscle serine1101-phosphorylation of IRS-1 was increased upon SAF and PAL consumption (p < 0.05), whereas PAL decreased serine473-phosphorylation of Akt more than SAF (p < 0.05).

Lipid-induced myocellular insulin resistance is likely more pronounced with palmitate than with oleate and is associated with PKC isoforms activation and inhibitory insulin signalling. β€’ Keywords: Glucose metabolism, Insulin signalling, Lipotoxicity, Monounsaturated fatty acids, Saturated fat, Skeletal muscle β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: DE Duesseldorf Roden M

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style  Pathology: Diabetes 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, NS  HRR: Oxygraph-2k, O2k-Fluorometer 

2021-10, AmR 

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