Template:SUIT text D012
From Bioblast
SUIT-003 O2 ce D012 is a coupling-control protocol for living cells. Different coupling control states are assessed (ROUTINE - L(Omy) - E) at ce-substrate state. The use of pyruvate is recommended to support the cell respiration if the incubation media is not cell culture media. The use of oligomycin provides important information about the ETS and TCA activity but the optimal concentration has to be carefully determined experimentally to avoid the known side effects over the ET-capacity. Rotenone and Antimycin A provide the ROX state and its sequential addition allows the extension of the protocol with the cell viability test module (See CCV SUIT-003 O2 ce-pce D020).
