| Year | Reference |
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MiPNet11.05 imt-pti-pce | 2022-04-10 | Isolated mitochondria or permeabilized tissues and cells. |
MiPNet22.10 MiR05-Kit | 2022-03-03 | Mitochondrial Respiration Medium - MiR05-Kit. |
Baglivo 2022 BEC | 2022 | Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. Bioenerg Commun 2022.8. https://doi.org/10.26124/bec:2022-0008 |
Baglivo 2022 MitoFit-QC | 2022 | Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. https://doi.org/10.26124/mitofit:2022-0018.v2 â 2022-08-04 published in Bioenerg Commun 2022.8. |
MiPNet24.10 H2O2 flux analysis | 2021-10-22 | Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4 |
MiPNet26.10 MgG data analysis | 2021-09-16 | Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4 |
MiPNet24.09 Data analysis of mt-membrane potential | 2021-09-16 | Data analysis of mt-membrane potential. |
MiPNet20.06 IsolationMouseHeart-mt | 2021-08-09 | Laboratory protocol: isolation of mouse heart mitochondria. |
MiPNet17.05 O2k-Fluo LED2-Module | 2021-06-22 | O2k-Fluo LED2-Module. |
Cardoso 2021 BEC MgG | 2021 | Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001 |
Went 2021 MitoFit PB | 2021 | Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-0005 |
Komlodi 2021 BEC Q | 2021 | KomlĂłdi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003 |
Komlodi 2021 BEC AmR-O2 | 2021 | KomlĂłdi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004 |
MiPNet21.17 BloodCellsIsolation | 2020-10-22 | O2k-Protocols: Isolation of blood cells for HRR. |
MiPNet09.12 O2k-Titrations | 2020-08-17 | O2k manual titrations: SUIT protocols with mitochondrial preparations. |
MiPNet24.08 Safranin Analysis Template | 2020-05-13 | Excel template for safranin data analysis. |
MiPNet17.04 CitrateSynthase | 2020-04-18 | Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. |
MiPNet24.06 Oxygen flux analysis - DatLab 7.4 | 2020-04-16 | Oxygen flux analysis with DatLab 7.4. |
MiPNet25.14 TPP Analysis Template | 2020-##-## | Excel template for TPP data analysis. |
BEC 2020.1 doi10.26124bec2020-0001.v1 | 2020 | Gnaiger E et al â MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 |
Gnaiger 2020 BEC MitoPathways | 2020 | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 |
MiPNet21.16 DatLab 7 Innovations | 2019-09-23 | DatLab 7: innovations. |
MiPNet23.12 DL-Protocols help | 2019-09-13 | DatLab Protocols: Help DatLab 7. |
MiPNet22.16 DatLab 7 Innovations: DL-Protocols | 2019-09-13 | DatLab 7.4 Innovations: DL-Protocols |
MiPNet24.11 mtMP calculation | 2019-08-05 | Mitochondrial membrane potential calculation |
MiPNet20.14 AmplexRed H2O2-production | 2019-06-24 | O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed. |
MiPNet20.13 Safranin mt-membranepotential | 2019-06-24 | O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM. |
MiPNet18.06 Amplex-Yeast | 2019-06-24 | O2k-FluoRespirometry: Amplex UltraRed using freeze-dried bakerâs yeast.
» Update: Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. MitoFit Preprints 2021.10. doi:10.26124/mitofit:2021-0010 |
MiPNet18.05 Amplex-Mouse-heart | 2019-06-24 | O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. |
Naszai 2019 Sci Rep | 2019 | NĂĄszai A, Terhes E, Kaszaki J, Boros M, JuhĂĄsz L (2019) Ca(2+)N it be measured? Detection of extramitochondrial calcium movement with high-resolution FluoRespirometry. Sci Rep 9:19229. |
Long 2019 Methods Mol Biol | 2019 | Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46. |
MiPNet12.01 Suppl T-issue | 2018-11-18 | MitoPathways at the Q-junction: mouse skeletal muscle fibers. |
Doerrier 2018 Methods Mol Biol | 2018 | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, MĂ©szĂĄros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 |
Komlodi 2018 Methods Mol Biol | 2018 | Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. |
Lemieux 2017 Sci Rep | 2017 | Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8 |
MiPNet20.08 IsolationRatLiver-mt | 2016-11-18 | Laboratory Protocol: isolation of rat liver mitochondria. |
MiPNet21.14 Reference sample HRR | 2016-10-19 | Development of a reference sample for HRR. |
MiPNet21.12 Comparison mt respiration media | 2016-10-18 | O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry. |
MiPNet21.13 Comparison mt-preparations | 2016-10-18 | O2k-Protocols: Mitochondrial preparations for HRR. |
MiPNet14.13 Medium-MiR06 | 2016-08-30 | Mitochondrial respiration medium - MiR06. |
MiPNet03.02 Chemicals-Media | 2016-08-30 | Selected media and chemicals for respirometry with mitochondrial preparations. |
MiPNet08.09 CellRespiration | 2016-08-11 | High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX. |
MiPNet19.18E O2 flux analysis - DatLab 6 | 2016-08-08 | Oxygen flux analysis: DatLab real-time. |
MiPNet10.04 CellRespiration | 2016-08-08 | An experiment with high-resolution respirometry: coupling control in cell respiration. |
MiPNet21.07 MitoFit DatLab PT | 2016-07-20 | MitoFit DatLab proficiency test. |
MiPNet20.07 IsolationRatBrain-mt | 2016-06-18 | Laboratory Protocol: isolation of rat brain mitochondria. |
MiPNet14.05 TPP-mtMembranePotential | 2016-04-01 | TPP+ and mt-membrane potential. |
MiPNet20.15 IsolationRatHeart-mt | 2016-03-25 | Laboratory protocol: isolation of rat heart mitochondria. |
MiPNet14.14 PermeabilizedFiberPreparation | 2015-06-22 | Preparation of permeabilized muscle fibers for diagnosis of mitochondrial respiratory function. |
MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver | 2015-06-22 | Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. |
MiPNet17.02 PBI-Shredder manual | 2015-06-19 | PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. |
MiPNet17.03 Shredder vs Fibers | 2015-06-18 | Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder. |
MiPNet20.09 IsolationBeefHeart-mt | 2015-06-15 | Laboratory protocol: isolation of beef heart mitochondria. |
Krumschnabel 2015 Methods Mol Biol | 2015 | Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. |
Makrecka-Kuka 2015 Biomolecules | 2015 | Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319 |
Gnaiger 2015 Scand J Med Sci Sports | 2015 | Gnaiger E, Boushel R, SĂžndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, DĂez-SĂĄnchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. https://doi.org/10.1111/sms.12612 |
MiPNet12.11 MitoRespiration | 2014-02-20 | Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control. |
Elustondo 2014 Biochim Biophys Acta | 2014 | Elustondo PA, Negoda A, Kane CL, Kane DA, Pavlov EV (2014) Spermine selectively inhibits high-conductance, but not low-conductance calcium-induced permeability transition pore. Biochim Biophys Acta 1847:231â40. |
Krumschnabel 2014 Methods Enzymol | 2014 | Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. https://doi.org/10.1016/B978-0-12-416618-9.00009-1 |
Van Bergen 2014 Mitochondrion | 2014 | Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33. |
Chinopoulos 2014 Methods Enzymol | 2014 | Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. doi:10.1016/B978-0-12-416618-9.00017-0 |
Pesta 2012 Methods Mol Biol | 2012 | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 |
MiPNet15.08 TPP electrode | 2011-12-11 | Ion selective electrode for TPP+ and high-resolution respirometry. |
MiPNet08.12 IOC22 | 2011-12-11 | NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. |
Lemieux 2011 Int J Biochem Cell Biol | 2011 | Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. https://doi.org/10.1016/j.biocel.2011.08.008 |
MiPNet08.15 Complex-I | 2010-08-23 | Laboratory protocol: Complex I (NADH:Ubiquinone Oxidoreductase, EC 1.6.5.3). Mitochondrial membrane enzyme. |
MiPNet08.18 LactateDehydrogenase | 2010-08-06 | Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. |
MiPNet08.13 mt-Isolation-RLM | 2010-08-03 | Laboratory protocol: Isolation of rat liver mitochondria. |
Aguirre 2010 Biochim Biophys Acta | 2010 | Aguirre E, RodrĂguez-JuĂĄrez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. https://doi.org/10.1016/j.bbabio.2010.01.033 |
Gnaiger 2009 Int J Biochem Cell Biol | 2009 | Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. https://doi.org/10.1016/j.biocel.2009.03.013 |
Gnaiger 2008 POS | 2008 | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. |
Gnaiger 2007 MitoPathways | 2007 |
Gnaiger E ed (2007) Mitochondrial pathways and respiratory control. 1st ed. Oroboros MiPNet Publications, Innsbruck:96 pp. ISBN 978-3-9502399-0-4. â see 5th edition: Gnaiger 2020 BEC MitoPathways. |
Huetter 2004 Biochem J | 2004 | HĂŒtter E, Renner K, Pfister G, Stöckl P, Jansen-DĂŒrr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. https://doi.org/10.1042/BJ20040095 |
Gnaiger 2000 Life in the Cold | 2000 | Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Berlin, Heidelberg:431-42. https://doi.org/10.1007/978-3-662-04162-8_45 |
MiPNet04.05 Titration-Injection | 1999-01 | From step titration to ramp injection: Uncoupling by FCCP with TIP. |
Steinlechner-Maran 1996 Am J Physiol Cell Physiol | 1996 | Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. |