Wilson 1970 Biochim Biophys Acta
| Wilson MA, Cascarano J (1970) The energy-yielding oxidation of NADH by fumarate in submitochondrial particles of rat tissues. Biochim Biophys Acta 216:54-62. doi: 10.1016/0005-2728(70)90158-1 |
Wilson MA, Cascarano J (1970) Biochim Biophys Acta
Abstract: 1. Oxidation of NADH by fumarate coupled to synthesis of ATP was found to occur in cyanide-poisoned rat heart submitochondrial particles. The reaction was inhibited by amytal, thenoyltrifluoroacetone and malonate, indicating the involvement of a portion of the electron transfer chain.
2. Cytochrome b became oxidized (while the other cytochromes remained reduced) during the oxidation of NADH by fumarate, suggesting that cytochrome b is part of the reaction pathway.
3. Succinate was recovered as the reaction product and accounted for the NADH oxidized.
4. The P/2e ratios indicate that one ATP was produced for each pair of electrons transferred to fumarate.
5. The reaction was also demonstrated to be present in liver and gastrocnemius muscle of rat. The reaction rate in heart was 2.0 times that of gastrocnemius and 3.3 times that of liver. These differences are not related to the activities of NADH or succinate dehydrogenase.
6. The ubiquitous nature of this reaction suggests that it could serve as an important physiological mechanism for generating extra glycolytic energy during periods of anoxia.
• Bioblast editor: Gnaiger E
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