Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Andreazza 2019 Nat Commun"

From Bioblast
Line 9: Line 9:
}}
}}
{{Labeling
{{Labeling
|area=Respiration, mtDNA;mt-genetics
|area=Respiration, mtDNA;mt-genetics, Genetic knockout;overexpression
|organism=Drosophila
|organism=Drosophila
|preparations=Homogenate
|preparations=Homogenate
Line 15: Line 15:
|pathways=N, S
|pathways=N, S
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|additional=Labels, 2019-08,
|additional=2019-08,
}}
}}

Revision as of 09:40, 9 August 2019

Publications in the MiPMap
Andreazza S, Samstag CL, Sanchez-Martinez A, Fernandez-Vizarra E, Gomez-Duran A, Lee JJ, Tufi R, Hipp MJ, Schmidt EK, Nicholls TJ, Gammage PA, Chinnery PF, Minczuk M, Pallanck LJ, Kennedy SR, Whitworth AJ (2019) Mitochondrially-targeted APOBEC1 is a potent mtDNA mutator affecting mitochondrial function and organismal fitness in Drosophila. Nat Commun 10:3280.

Β» PMID: 31337756 Open Access

Andreazza S, Samstag CL, Sanchez-Martinez A, Fernandez-Vizarra E, Gomez-Duran A, Lee JJ, Tufi R, Hipp MJ, Schmidt EK, Nicholls TJ, Gammage PA, Chinnery PF, Minczuk M, Pallanck LJ, Kennedy SR, Whitworth AJ (2019) Nat Commun

Abstract: Somatic mutations in the mitochondrial genome (mtDNA) have been linked to multiple disease conditions and to ageing itself. In Drosophila, knock-in of a proofreading deficient mtDNA polymerase (POLG) generates high levels of somatic point mutations and also small indels, but surprisingly limited impact on organismal longevity or fitness. Here we describe a new mtDNA mutator model based on a mitochondrially-targeted cytidine deaminase, APOBEC1. mito-APOBEC1 acts as a potent mutagen which exclusively induces C:G>T:A transitions with no indels or mtDNA depletion. In these flies, the presence of multiple non-synonymous substitutions, even at modest heteroplasmy, disrupts mitochondrial function and dramatically impacts organismal fitness. A detailed analysis of the mutation profile in the POLG and mito-APOBEC1 models reveals that mutation type (quality) rather than quantity is a critical factor in impacting organismal fitness. The specificity for transition mutations and the severe phenotypes make mito-APOBEC1 an excellent mtDNA mutator model for ageing research.

β€’ Bioblast editor: Plangger M


Labels: MiParea: Respiration, mtDNA;mt-genetics, Genetic knockout;overexpression 


Organism: Drosophila 

Preparation: Homogenate 


Coupling state: OXPHOS  Pathway: N, S  HRR: Oxygraph-2k 

2019-08