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Difference between revisions of "Ap5A"

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|description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
|description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
}}
}}
__TOC__
== Application in [[HRR]] ==
{{Chemical_description
|abbr=Ap5A
|trivial name=Ap5A
|complete name=P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt
|chem formula=C<sub>2</sub>0H<sub>29</sub>N<sub>10</sub>O<sub>22</sub>P<sub>5</sub> · 5 Na
|molar mass=1026.28
|vendor=Sigma-Aldrich
|product number=D4022
|store at=-20 °C
|sensitivity=
|cas=4097-04-5
|h statements=
|h info=
}}<!--:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28
::::  <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>-->
:::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O)
::::# Dissolve content of Sigma 2 mg glass vial in 19.49 µL H<sub>2</sub>O
::::# Store at -20 °C.
:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
::::* Final concentration: 50 µM.
== References ==
::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980]
::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014]
{{MitoPedia concepts}}
{{MitoPedia concepts}}
{{MitoPedia methods}}
{{MitoPedia methods}}
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|mitopedia topic=Inhibitor
|mitopedia topic=Inhibitor
}}
}}
__TOC__
== Application in [[HRR]] ==
:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28
::::  <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>
:::: '''Preparation of 100 mM stock solution''' (dissolved in H2O)
:::::# For a 2 mg glass vial: dissolve in 19.49 µL H2O::::# Store at -20 °C.
:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber).
::::* Final concentration: 50 µM.== References ==
::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980]
::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014]

Latest revision as of 09:25, 21 June 2021


high-resolution terminology - matching measurements at high-resolution


Ap5A

Description

P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).

Abbreviation: Ap5A


Application in HRR

Ap5A: Ap5A (P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt; C20H29N10O22P5 · 5 Na), Sigma-Aldrich: D4022, store at -20 °C, CAS: 4097-04-5, M = 1026.28 g·mol-1


Preparation of 100 mM stock solution (dissolved in H2O)
  1. Dissolve content of Sigma 2 mg glass vial in 19.49 µL H2O
  2. Store at -20 °C.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
  • Final concentration: 50 µM.


References






MitoPedia topics: Inhibitor