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Difference between revisions of "Ap5A"

From Bioblast
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|abbr=Ap5A
|abbr=Ap5A
|description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
|description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
 
}}
{{MitoPedia concepts}}
{{MitoPedia methods}}
{{MitoPedia O2k and high-resolution respirometry}}
{{MitoPedia topics
|mitopedia topic=Inhibitor
}}
__TOC__
__TOC__
== Application in [[HRR]] ==
== Application in [[HRR]] ==
:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28
:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28
::::  <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>
::::  <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>
 
:::: '''Preparation of 100 mM stock solution''' (dissolved in H2O)
 
:::::# For a 2 mg glass vial: dissolve in 19.49 µL H2O::::# Store at -20 °C.
 
:::: '''Preparation of 100 mM stock solution''' (dissolved in H2O):
 
::::# For a 2 mg glass vial: dissolve in 19.49 µL H2O
::::# Store at -20 °C.
 
 
:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber).
::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber).
::::* Final concentration: 50 µM.
::::* Final concentration: 50 µM.== References ==
 
 
 
== References ==
::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980]
::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980]
::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014]
::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014]
}}
{{MitoPedia topics
|mitopedia topic=Inhibitor
}}

Revision as of 14:20, 30 November 2020


high-resolution terminology - matching measurements at high-resolution


Ap5A

Description

P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).

Abbreviation: Ap5A






MitoPedia topics: Inhibitor 

Application in HRR

Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt Sigma D4022, store at -20 °C; molecular weight: 1026.28
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 100 mM stock solution (dissolved in H2O)
  1. For a 2 mg glass vial: dissolve in 19.49 µL H2O::::# Store at -20 °C.
» O2k manual titrations MiPNet09.12 O2k-Titrations