Difference between revisions of "Ap5A"
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|abbr=Ap5A | |abbr=Ap5A | ||
|description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP). | |description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP). | ||
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__TOC__ | __TOC__ | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28 | :::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28 | ||
:::: <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | :::: <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
:::: '''Preparation of 100 mM stock solution''' (dissolved in H2O) | |||
:::::# For a 2 mg glass vial: dissolve in 19.49 µL H2O::::# Store at -20 °C. | |||
:::: '''Preparation of 100 mM stock solution''' (dissolved in H2O): | |||
::::# For a 2 mg glass vial: dissolve in 19.49 µL H2O | |||
::::# Store at -20 °C. | |||
:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | :::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber). | ::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber). | ||
::::* Final concentration: 50 µM. | ::::* Final concentration: 50 µM. | ||
== References == | == References == | ||
::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980] | ::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980] | ||
::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014] | ::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014] | ||
}} | |||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
Revision as of 15:20, 30 November 2020
- high-resolution terminology - matching measurements at high-resolution
Ap5A
Description
P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
Abbreviation: Ap5A
Application in HRR
- Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt Sigma D4022, store at -20 °C; molecular weight: 1026.28
- Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Preparation of 100 mM stock solution (dissolved in H2O)
- For a 2 mg glass vial: dissolve in 19.49 µL H2O::::# Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 50 µM.
References
MitoPedia topics: Inhibitor
