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Difference between revisions of "Arruda 2008 Endocrinology"

From Bioblast
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{{Labeling
{{Labeling
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|organism=Mammals
|organism=Rabbit
|tissues=Skeletal muscle
|tissues=Skeletal muscle
|couplingstates=OXPHOS, ETS
|couplingstates=OXPHOS, ETS

Revision as of 12:09, 14 March 2013

Publications in the MiPMap
Arruda AP, Ketzer LA, Nigro M, Galina A, Carvalho DP, de Meis L (2008) Cold tolerance in hypothyroid rabbits: role of skeletal muscle mitochondria and sarcoplasmic reticulum Ca2+ ATPase isoform 1 heat production. Endocrinol 149: 6262-6271.

Β» PMID: 18703625

Arruda AP, Ketzer LA, Nigro M, Galina A, Carvalho DP, de Meis L (2008) Endocrinol

Abstract: Brown adipose tissue (BAT) is involved in rat and mice thermoregulation, and heat produced by BAT depends on the concerted action of thyroid hormones and catecholamines. Little is known about cold-induced thermogenesis in mammals that have little or no BAT, such as rabbits. In these animals, thermogenesis primarily occurs in skeletal muscle. In this work, we have studied the effect of cold acclimation (4 C for 10 d) in normal and hypothyroid rabbits. It is known that hypothyroid rats die after a few hours of cold exposure. We now show that, different from rats, hypothyroid rabbits sustain their body temperature and survive after 10 d cold exposure. When compared with rabbits kept at room temperature, the muscles of cold-exposed rabbits showed a dark red color characteristic of oxidative muscle fibers. According to this pattern, we observed that in both normal and hypothyroid rabbits, cold exposure promotes an increase in oxygen consumption by skeletal muscle mitochondria. Moreover, in red muscle, cold acclimation induces an increase in the expression and activity of sarcoplasmic reticulum Ca2+ ATPase isoform 1 (SERCA1), one of the muscle enzymes involved in heat production. We conclude that rabbit cold tolerance is probably related to increased muscle oxidative metabolism and heat production by SERCA1 and that these changes are not completely dependent on normal thyroid function. β€’ Keywords: Brown adipose tissue, Skeletal muscle, Thermoregulation, MitoMed2, Rabbits

β€’ O2k-Network Lab: BR_Rio de Janeiro_Galina A, BR Rio de Janeiro Institute Biomedical Chemistry


Labels:


Organism: Rabbit  Tissue;cell: Skeletal muscle 


Regulation: Ion Homeostasis"Ion Homeostasis" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.  Coupling state: OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k