Bioblast alert 2011

Bioblast alert 2011

Bioblast alert is prepared by the Bioblast editorial board to draw attention to specific topics on the Bioblast wiki.

Bioblast alert: for information purposes, not intended to be an advertisement.  

Bioblast alert 2011: 01 to 04 >> Current issue

Bioblast alert 2011(01): 2011-11-11

• A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?

Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.

• ETS capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
• Basal respiration - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.

Bioblast alert 2011(02): 2011-11-17

• IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India: 2011-12-08.

• Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.

Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738. --> Discussion: Complementary results on the PGC-1a pathway.

• Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols.

• Uncoupled, noncoupled, dyscoupled - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology.

Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.

Bioblast alert 2011(03): 2011-11-24

• Fluorometry combined with high-resolution respirometry: Light guide inserted into the chamber through a black PEEK stopper, or optical probe operated through the O2k-glass window.

• Fluorometry versus fluorimetry - term of the week. >>>New MitoPedia: Fluorometry - high-resolution terminology.

Bioblast alert 2011(04): 2011-12-24

• Congratulations to Dr. Dominik Pesta, for receiving his degree of a PhD at the Medical University of Innsbruck! (Jan 2012)
• Bioblast editorial board - Why a wiki editorial board?
• SUIT protocols for HRR: from intact cells to permeabilized muscle fibres:

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58.