Brewer 2004 Mitochondrion
| Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with anti-oxidants. Mitochondrion 4:49-57. |
Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Mitochondrion
Abstract: We tested the effect of an anti-oxidant mixture on respiration in isolated rat brain mitochondria. Mitochondria were isolated in mannitol/sucrose/EGTA/BSA±SCAVEGR™ anti-oxidants (SOD, catalase, vitamin E, vitamin E acetate, and glutathione reduced). TBARS were reduced by greater than 40% with SCAVEGR. Respiration driven by ADP showed a two-fold higher Vmax and a 15% higher respiratory control ratio when mitochondria were prepared with SCAVEGR. SCAVEGR also stabilized the octameric state of mitochondrial creatine kinase and thus improved creatine-stimulated respiration. These results suggest that significant improvements in brain mitochondrial function are obtained by isolation in the presence of an anti-oxidants mixture. • Keywords: Anti-oxidant, Brain mitochondria, Creatine kinase, Respiration, ROS
• O2k-Network Lab: CH Zurich Wallimann T, US IL Springfield Brewer GJ, FR Grenoble Schlattner U
Cited by
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
Labels:
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Nervous system Preparation: Isolated mitochondria
HRR: Oxygraph-2k
BEC 2020.2
