Buck 1993 Am J Physiol Regul Integr Comp Physiol

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Buck LT, Hochachka PW, SchΓΆn A, Gnaiger E (1993) Microcalorimetric measurement of reversible metabolic suppression induced by anoxia in isolated hepatocytes. Am J Physiol Regul Integr Comp Physiol 265:R1014-9.

Β» PMID: 8238601

Buck LT, Hochachka PW, Schoen A, Gnaiger Erich (1993) Am J Physiol Regul Integr Comp Physiol

Abstract: The metabolic suppression due to anoxia in hepatocytes from the anoxia-tolerant turtle Chrysemys picta bellii was measured directly using microcalorimetric techniques. The normoxic heat flux from hepatocytes in suspension (25 Β°C) was 1.08 +/- 0.08 mW/g cells and decreased by 76% to 0.26 +/- 0.03 mW/g cells in response to anoxic incubation. After an acute decrease in temperature (to 10 Β°C) anoxic heat flux dropped by 96% relative to the normoxic control at 25 degrees C. The relative decrease in heat flux at both temperatures was similar, 76% at 25 Β°C and 68% at 10 Β°C. From the caloric equivalent of glycogen fermentation to lactate the heat flux from lactate production was calculated to be -93 Β΅W/g cells (25 Β°C), and this accounted for 36% of the anoxic heat flux. When the enthalpy change associated with the release of free glucose (from glycogen breakdown) is considered, an additional 6% of the anoxic heat flux can be accounted for. Therefore, a portion of the anoxic heat flux is unaccounted for (58%), resulting in an "exothermic gap." This differs from the normoxically incubated hepatocytes where the indirect calorimetric measurement of heat flux (hepatocyte O2 consumption) could fully account for the calorimetrically measured heat flux. When normoxic hepatocytes were inhibited with cyanide, a rapid suppression in heat flux was observed. Because rapid reequilibration to a lower, cyanide-induced steady state occurred in < 15 min, it is also assumed that there is no short-term Pasteur effect in this tissue. (ABSTRACT TRUNCATED AT 250 WORDS) β€’ Keywords: Direct and Indirect Calorimetry, Enthalpy, Pasteur effect, Chrysemys picta bellii turtle

β€’ O2k-Network Lab: AT Innsbruck Gnaiger E

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Labels: MiParea: Respiration, Comparative MiP;environmental MiP 

Stress:Ischemia-reperfusion  Organism: Reptiles  Tissue;cell: Liver  Preparation: Intact cells 

Coupling state: LEAK, ROUTINE 

HRR: Oxygraph-2k 


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