Difference between revisions of "Chicco 2013 Abstract MiP2013"
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|abstract= | |abstract=The Northern Elephant seal (Mirounga angustirostis; E-seal) is known for its remarkable capacity for diving in cold water to hunt for up to 2 hours without resurfacing for air. E-seal muscle contains very large quantities of myoglobin, which helps to maintain muscle pO2 prior to and during long dives, but the muscle mitochondrial respiratory phenotype of this species has not been characterized. Ongoing studies in our lab have sought to understand the effects of species, sex and environment on muscle mitochondrial function in E-seals by performing high resolution respirometry (HRR) on samples obtained from individuals at various stages of their life cycle, compared to data obtained from human vastus lateralis muscle biopsies in our laboratory. Seal biopsies were taken from the primary swimming muscle (M. longissimus dorsi) with a 6 mm biopsy cannula and stored on ice-cold BIOPS (0-4 days) prior to being saponin permeabilized for HRR using a variety of SUIT protocols on an Oroboros Oxygraph-2k. | ||
|mipnetlab=US CO Fort Collins Chicco AJ | |||
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|injuries=Hypoxia | |||
|organism=Human | |||
|taxonomic group=Mammals | |||
|tissues=Skeletal muscle | |||
|preparations=Permeabilized tissue | |||
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Revision as of 21:48, 6 July 2013
| Chicco AJ, Le CH, Schlater A, Kanatous S (2013) Comparative muscle mitochondrial physiology of the northern elephant seal. Mitochondr Physiol Network 18.08. |
Link:
Chicco AJ, Le CH, Schlater A, Kanatous S (2013)
Event: MiP2013
The Northern Elephant seal (Mirounga angustirostis; E-seal) is known for its remarkable capacity for diving in cold water to hunt for up to 2 hours without resurfacing for air. E-seal muscle contains very large quantities of myoglobin, which helps to maintain muscle pO2 prior to and during long dives, but the muscle mitochondrial respiratory phenotype of this species has not been characterized. Ongoing studies in our lab have sought to understand the effects of species, sex and environment on muscle mitochondrial function in E-seals by performing high resolution respirometry (HRR) on samples obtained from individuals at various stages of their life cycle, compared to data obtained from human vastus lateralis muscle biopsies in our laboratory. Seal biopsies were taken from the primary swimming muscle (M. longissimus dorsi) with a 6 mm biopsy cannula and stored on ice-cold BIOPS (0-4 days) prior to being saponin permeabilized for HRR using a variety of SUIT protocols on an Oroboros Oxygraph-2k.
• O2k-Network Lab: US CO Fort Collins Chicco AJ
Labels:
Stress:Hypoxia Organism: Human Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
HRR: Oxygraph-2k
Affiliations and author contributions
1 - Mitochondrial Physiology Laboratory, Department of Health and Exercise Science;
2 - Cell and Molecular Biology Program; 3Department of Biology, Colorado State University, Fort Collins, CO, USA.
Email:
References
- Butler PJ (2004) Metabolic regulation in diving birds and mammals. Respir Physiol Neurobiol 141: 297-315.
- Kelso EJ, Champagne CD, Tift MS, Houser DS, Crocker DE (2012) Sex differences in fuel use and metabolism during development in fasting juvenile northern elephant seals. J Exp Biol 215: 2637-2645.
