Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Coupling-control protocol"

From Bioblast
Line 2: Line 2:
|abbr=CCP
|abbr=CCP
|description=A '''coupling control protocol, CCP''', induces different [[coupling control state]]s at constant substrate supply. In [[intact cell]]s, the CCP can be applied by using membrane-permeable inhibitors of the [[phosphorylation system]] (e.g. [[oligomycin]]) and [[uncoupler]]s (e.g. [[CCCP]]). Coupling control states in intact cells include ''R'', ''L'', ''E''; [[LEAK]], [[ROUTINE]], and [[ETS]]. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include ''L'', ''P'', ''E''; LEAK, [[OXPHOS]], and ETS. The term '''phosphorylation control protocol''', PCP, has been introduced synonymous for CCP.
|description=A '''coupling control protocol, CCP''', induces different [[coupling control state]]s at constant substrate supply. In [[intact cell]]s, the CCP can be applied by using membrane-permeable inhibitors of the [[phosphorylation system]] (e.g. [[oligomycin]]) and [[uncoupler]]s (e.g. [[CCCP]]). Coupling control states in intact cells include ''R'', ''L'', ''E''; [[LEAK]], [[ROUTINE]], and [[ETS]]. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include ''L'', ''P'', ''E''; LEAK, [[OXPHOS]], and ETS. The term '''phosphorylation control protocol''', PCP, has been introduced synonymous for CCP.
Β» [[#From PCP to CCP |'''MiPNet article''']]
Β» [[Coupling_control_protocol#From_PCP_to_CCP |'''MiPNet article''']]
|info=[[Gnaiger 2008 POS]], [[Gnaiger 2014 MitoPathways]]
|info=[[Gnaiger 2008 POS]], [[Gnaiger 2014 MitoPathways]]
}}
}}

Revision as of 16:22, 26 August 2015


high-resolution terminology - matching measurements at high-resolution


Coupling-control protocol

Description

A coupling control protocol, CCP, induces different coupling control states at constant substrate supply. In intact cells, the CCP can be applied by using membrane-permeable inhibitors of the phosphorylation system (e.g. oligomycin) and uncouplers (e.g. CCCP). Coupling control states in intact cells include R, L, E; LEAK, ROUTINE, and ETS. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include L, P, E; LEAK, OXPHOS, and ETS. The term phosphorylation control protocol, PCP, has been introduced synonymous for CCP. Β» MiPNet article

Abbreviation: CCP

Reference: Gnaiger 2008 POS, Gnaiger 2014 MitoPathways


MitoPedia methods: Respirometry 


MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 

From PCP to CCP

Publications in the MiPMap
Gnaiger E (2015) From PCP to CCP. Mitochondr Physiol Network 2015-01-11.


OROBOROS (2015) MiPNet

Abstract: The control of oxidative phosphorylation by coupling is of primary importance in OXPHOS analysis. To avoid misunderstandings, it is suggested to replace the term 'phosphorylation control protocol' by coupling control protocol, CCP.


β€’ O2k-Network Lab: AT Innsbruck Gnaiger E


Labels:




Regulation: Coupling efficiency;uncoupling  Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Theory 


In functional OXPHOS analysis, the control of oxidative phosphorylation by coupling is of primary importance, as studied by application of protocols, in which the phosphorylation system is either inhibited (lack of ADP; inhibition by oligomycin), activated (saturating ADP; activation by phyiological control in intact cells) or eliminated (uncoupling). It seems thus appropriate to use the term phosphorylation control protocol, PCP [1]. In this context it is taken for granted that we do not refer to metabolic control by phosphorylation of enzymes, which is an important mechanism to change specific enzyme activity. To avoid any such confusion, it is suggested to replace the term 'phosphorylation control protocol' by coupling control protocol, CCP [2]


ROUTINE - LEAK - ETS - ROX: this is the coupling control protocol with intact cells (ROUTINE - LEAKOmy - ETS - ROX).

OXPHOS - LEAK - ETS - ROX: this is the comparable coupling control protocol with mitochondrial preparations (OXPHOS - LEAKOmy - ETS - ROX).

LEAK - OXPHOS - LEAK - OXPHOS - ETS - ROX: In mitochondrial preparations, various variations are possible for the coupling control protocol, for example in isolated mitochondria [3]: (LEAKN - OXPHOS - LEAKT - OXPHOS - ETS - ROX).[4]

References

  1. ↑ Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. Β»Bioblast AccessΒ« 
  2. ↑ Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. Β»Open AccessΒ« 
  3. ↑ Hand SC, Gnaiger E (2014) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11(06):1-5. Β»Open AccessΒ« 
  4. ↑ LEAK respiration

Biochemical coupling efficiency: from 0 to <1


O2k-Publications in the MiPMap

O2k-Publications: coupling control protocol with intact cells

Sort in ascending/descending order by a click on one of the small symbols in squares below.
Default sorting: chronological. Empty fields appear first in ascending order. 


O2k-Publications in the MiPMap

O2k-Publications: coupling control protocol with intact cells - Abstracts

Sort in ascending/descending order by a click on one of the small symbols in squares below.