Difference between revisions of "Dejean 2002 Biochem Biophys Res Comm"
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|abstract=We investigated the effects of genetic and physiological modulations of the cAMP–protein kinase A pathway on mitochondrial biogenesis of yeast cells grown on lactate. Yeast mutants with over-activated Ras/adenylate cyclase pathway (i.e., ''Ras2<sup>val19</sup>, ira1Δira2Δ'') or with a constitutive downstream activation of protein kinases A (i.e., bcyΔ) showed an increase in the mitochondrial enzyme content. In contrast, loss of Ras activity (i.e., ''Ras2'' mutant) resulted in a slight decrease. The treatment by cAMP of a responsive mutant increased the oxidative phosphorylation capacity of cells and increased the transcript level of nuclear genes encoding for mitochondrial proteins. In contrast, the transcript level of mitochondrial DNA genes was unchanged. It is concluded that the Ras/cAMP/protein kinase A pathway is part of the regulatory circuit controlling biogenesis of the oxidative phosphorylation complexes in yeast cells. | |abstract=We investigated the effects of genetic and physiological modulations of the cAMP–protein kinase A pathway on mitochondrial biogenesis of yeast cells grown on lactate. Yeast mutants with over-activated Ras/adenylate cyclase pathway (i.e., ''Ras2<sup>val19</sup>, ira1Δira2Δ'') or with a constitutive downstream activation of protein kinases A (i.e., bcyΔ) showed an increase in the mitochondrial enzyme content. In contrast, loss of Ras activity (i.e., ''Ras2'' mutant) resulted in a slight decrease. The treatment by cAMP of a responsive mutant increased the oxidative phosphorylation capacity of cells and increased the transcript level of nuclear genes encoding for mitochondrial proteins. In contrast, the transcript level of mitochondrial DNA genes was unchanged. It is concluded that the Ras/cAMP/protein kinase A pathway is part of the regulatory circuit controlling biogenesis of the oxidative phosphorylation complexes in yeast cells. | ||
|keywords=cAMP, ''Saccharomyces cerevisiae'', Ras mutants, Mitochondria, Cytochromes, Respiration | |keywords=cAMP, ''Saccharomyces cerevisiae'', Ras mutants, Mitochondria, Cytochromes, Respiration | ||
|mipnetlab= | |mipnetlab=FR_Pessac_Beauvoit B, FR Bordeaux Devin A | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Revision as of 16:53, 12 March 2013
| Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M (2002) Activation of Ras cascade increases the mitochondrial enzyme content of respiratory competent yeast. Biochem Biophys Res Comm 293: 1383-1388. |
Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M (2002) Biochem Biophys Res Commun
Abstract: We investigated the effects of genetic and physiological modulations of the cAMP–protein kinase A pathway on mitochondrial biogenesis of yeast cells grown on lactate. Yeast mutants with over-activated Ras/adenylate cyclase pathway (i.e., Ras2val19, ira1Δira2Δ) or with a constitutive downstream activation of protein kinases A (i.e., bcyΔ) showed an increase in the mitochondrial enzyme content. In contrast, loss of Ras activity (i.e., Ras2 mutant) resulted in a slight decrease. The treatment by cAMP of a responsive mutant increased the oxidative phosphorylation capacity of cells and increased the transcript level of nuclear genes encoding for mitochondrial proteins. In contrast, the transcript level of mitochondrial DNA genes was unchanged. It is concluded that the Ras/cAMP/protein kinase A pathway is part of the regulatory circuit controlling biogenesis of the oxidative phosphorylation complexes in yeast cells. • Keywords: cAMP, Saccharomyces cerevisiae, Ras mutants, Mitochondria, Cytochromes, Respiration
• O2k-Network Lab: FR_Pessac_Beauvoit B, FR Bordeaux Devin A
Labels:
Stress:Genetic Defect; Knockdown; Overexpression"Genetic Defect; Knockdown; Overexpression" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Saccharomyces cerevisiae, Yeast; Fungi"Yeast; Fungi" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Coupling state: OXPHOS
HRR: Oxygraph-2k
