Difference between revisions of "Digitonin"
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|description='''Digitonin''' is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations.Β Optimum digitonin concentrations for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol. | |description='''Digitonin''' is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations.Β Optimum digitonin concentrations for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
|type=Chemicals | |||
}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Permeabilization agent | |||
|type=Chemicals | |type=Chemicals | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Chemicals; Media | |instruments=Chemicals; Media | ||
|type=Chemicals | |||
}} | }} |
Revision as of 13:36, 9 March 2011
Description
Digitonin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Optimum digitonin concentrations for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol.
Abbreviation: Dig
Reference: MiPNet09.12
MitoPedia topics:
Permeabilization agent
Labels:
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.