Difference between revisions of "Digitonin"
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== Application in [[HRR]] == | |||
'''Dig: Digitonin''' (C<sub>56</sub>H<sub>92</sub>O<sub>29</sub>; FW = 1229.31); Fluka 37008, 1 g, store at R.T.; FW = 1229.31 | |||
<span style="color:#8B008B"> '''Caution:''' Toxic!</span> | |||
'''Comment:''' ''The product is withdrawn from Sigma!'' | |||
'''Preparation of 8.1 mM stock solution (dissolved in DMSO)''': | |||
::1) weigh 10 mg of Digitonin | |||
::2) dissolve in 1 ml DMSO | |||
::3) store frozen at -20 °C | |||
Revision as of 11:42, 18 May 2012
- high-resolution terminology - matching measurements at high-resolution
Digitonin
Description
Digitonin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Optimum digitonin concentrations for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol.
Abbreviation: Dig
Reference: MiPNet09.12
MitoPedia topics:
Permeabilization agent
Application in HRR
Dig: Digitonin (C56H92O29; FW = 1229.31); Fluka 37008, 1 g, store at R.T.; FW = 1229.31
Caution: Toxic!
Comment: The product is withdrawn from Sigma!
Preparation of 8.1 mM stock solution (dissolved in DMSO):
- 1) weigh 10 mg of Digitonin
- 2) dissolve in 1 ml DMSO
- 3) store frozen at -20 °C
