Difference between revisions of "Digitonin"
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::2) dissolve in 1 ml DMSO | ::2) dissolve in 1 ml DMSO | ||
::3) store frozen at -20 °C | ::3) store frozen at -20 °C | ||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | |||
::* Titration volume: 1 µl using a 10 µl syringe | |||
::* Final conc. in 2 ml O2k-chamber: 4.07 µM | |||
Revision as of 17:29, 20 November 2013
- high-resolution terminology - matching measurements at high-resolution
Digitonin
Description
Digitonin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Optimum digitonin concentrations for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol.
Abbreviation: Dig
Reference: MiPNet09.12
MitoPedia topics:
Permeabilization agent
Application in HRR
Dig: Digitonin (C56H92O29); Sigma D 5628, 1 g, store at R.T.; FW = 1229.31
Caution: Toxic!
Preparation of 8.1 mM stock solution (dissolved in DMSO):
- 1) weigh 10 mg of Digitonin
- 2) dissolve in 1 ml DMSO
- 3) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 4.07 µM
