Difference between revisions of "Gain"
From Bioblast
| Line 9: | Line 9: | ||
:::: For most applications between 25 and 37 °C the pre-set gain of 1 (default) is adequate and does not have to be changed. | :::: For most applications between 25 and 37 °C the pre-set gain of 1 (default) is adequate and does not have to be changed. | ||
:::: The gain of the signal of the O<sub>2</sub> channel can be adjusted in DatLab by selecting [ | :::: The gain of the signal of the O<sub>2</sub> channel can be adjusted in DatLab by selecting [Oroboros O2k] / [O2k Control] / Tab [Oxygen, O2] from the menu. When the gain setting is too high for any given signal, it will reach a constant maximum value of 10 V (often displayed as 9.99 V) and the displayed slope will therefore apparently be constant at zero. | ||
:::: [[File:Gain setting too high.png|600px|MitoFit-QCS]] | :::: [[File:Gain setting too high.png|600px|MitoFit-QCS]] | ||
:::: For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment) | :::: For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment) but also a new zero calibration, see [[Air calibration]]. If for any reason you frequently switch between gains, the easiest way is to store the calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually. | ||
:::: Oxygen signals that have been obtained after proper calibration are independent of the gain setting. Therefore oxygen concentrations (and fluxes) that have been recorded at different gain settings can of course be used together. | :::: Oxygen signals that have been obtained after proper calibration are independent of the gain setting. Therefore oxygen concentrations (and fluxes) that have been recorded at different gain settings can of course be used together, as long as the proper calibration is applied for these files. | ||
:::: See also »[[Oxygen signal]] | :::: See also »[[Oxygen signal]] | ||
Revision as of 16:06, 30 November 2020
- high-resolution terminology - matching measurements at high-resolution
Gain
Description
The gain is an amplification factor applied to an input signal to increase the output signal.
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Gain: O2 channel of the O2k
- For most applications between 25 and 37 °C the pre-set gain of 1 (default) is adequate and does not have to be changed.
- The gain of the signal of the O2 channel can be adjusted in DatLab by selecting [Oroboros O2k] / [O2k Control] / Tab [Oxygen, O2] from the menu. When the gain setting is too high for any given signal, it will reach a constant maximum value of 10 V (often displayed as 9.99 V) and the displayed slope will therefore apparently be constant at zero.
- For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment) but also a new zero calibration, see Air calibration. If for any reason you frequently switch between gains, the easiest way is to store the calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually.
- Oxygen signals that have been obtained after proper calibration are independent of the gain setting. Therefore oxygen concentrations (and fluxes) that have been recorded at different gain settings can of course be used together, as long as the proper calibration is applied for these files.
- See also »Oxygen signal
Gain: pX channel of the O2k
Gain: NO channel of the O2k
MitoPedia O2k and high-resolution respirometry: O2k hardware, DatLab
