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Difference between revisions of "Gizatullina 2005 Biochim Biophys Acta"

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{{Publication
{{Publication
|title=Gizatullina ZZ, Chen Y, Zierz S, Gellerich FN (2005) Effects of extramitochondrial ADP on permeability transition of mouse liver mitochondria. Biochim. Biophys. Acta 1706: 98-104.
|title=Gizatullina ZZ, Chen Y, Zierz S, Gellerich FN (2005) Effects of extramitochondrial ADP on permeability transition of mouse liver mitochondria. Biochim Biophys Acta 1706:98-104.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/15620369 PMID: 15620369 Open Access]
|authors=Gizatullina ZZ, Chen Y, Zierz S, Gellerich FN
|authors=Gizatullina ZZ, Chen Y, Zierz S, Gellerich FN
|year=2005
|year=2005
|journal=Biochim. Biophys. Acta
|journal=Biochim Biophys Acta
|abstract=Carboxyatractylate (CAT) and atractylate inhibit the mitochondrial adenine nucleotide translocator (ANT) and stimulate the opening of permeability transition pore (PTP). Following pretreatment of mouse liver mitochondria with 5 μM CAT and 75 μM Ca<sup>2+</sup>, the activity of PTP increased, but addition of 2 mM ADP inhibited the swelling of mitochondria. Extramitochondrial Ca2+ concentration measured with Calcium-Green 5N evidenced that 2 mM ADP did not remarkably decrease the free Ca<sup>2+</sup> but the release of Ca<sup>2+</sup> from loaded mitochondria was stopped effectively after addition of 2 mM ADP. CAT caused a remarkable decrease of the maximum amount of calcium ions, which can be accumulated by mitochondria. Addition of 2 mM ADP after 5 μM CAT did not change the respiration, but increased the mitochondrial capacity for Ca<sup>2+</sup> at more than five times. Bongkrekic acid (BA) had a biphasic effect on PT. In the first minutes 5 μM BA increased the stability of mitochondrial membrane followed by a pronounced opening of PTP too. BA abolished the action about of 1 mM ADP, but was not able to induce swelling of mitochondria in the presence of 2 mM ADP.
|abstract=Carboxyatractylate (CAT) and atractylate inhibit the mitochondrial adenine nucleotide translocator (ANT) and stimulate the opening of permeability transition pore (PTP). Following pretreatment of mouse liver mitochondria with 5 μM CAT and 75 μM Ca<sup>2+</sup>, the activity of PTP increased, but addition of 2 mM ADP inhibited the swelling of mitochondria. Extramitochondrial Ca2+ concentration measured with Calcium-Green 5N evidenced that 2 mM ADP did not remarkably decrease the free Ca<sup>2+</sup> but the release of Ca<sup>2+</sup> from loaded mitochondria was stopped effectively after addition of 2 mM ADP. CAT caused a remarkable decrease of the maximum amount of calcium ions, which can be accumulated by mitochondria. Addition of 2 mM ADP after 5 μM CAT did not change the respiration, but increased the mitochondrial capacity for Ca<sup>2+</sup> at more than five times. Bongkrekic acid (BA) had a biphasic effect on PT. In the first minutes 5 μM BA increased the stability of mitochondrial membrane followed by a pronounced opening of PTP too. BA abolished the action about of 1 mM ADP, but was not able to induce swelling of mitochondria in the presence of 2 mM ADP.


We conclude that the outer side of inner mitochondrial membrane has a low affinity sensor for ADP, modifying the activity of PTP. The pathophysiological importance of this process could be an endogenous prevention of PT at conditions of energetic depression.
We conclude that the outer side of inner mitochondrial membrane has a low affinity sensor for ADP, modifying the activity of PTP. The pathophysiological importance of this process could be an endogenous prevention of PT at conditions of energetic depression.
|keywords=Mitochondria, Permeability transition pore, Adenine nucleotide translocator, Carboxyatractyloside,  Bongkrekic acid
|keywords=Mitochondria, Permeability transition pore, Adenine nucleotide translocator, Carboxyatractyloside,  Bongkrekic acid
|mipnetlab=DE Magdeburg Gellerich FN,
|mipnetlab=DE Magdeburg Gellerich FN
}}
}}
{{Labeling
{{Labeling
|organism=Mouse
|tissues=Liver
|preparations=Isolated mitochondria
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|organism=Mouse
|tissues=Hepatocyte; Liver
|preparations=Isolated Mitochondria
}}
}}

Latest revision as of 14:07, 24 March 2015

Publications in the MiPMap
Gizatullina ZZ, Chen Y, Zierz S, Gellerich FN (2005) Effects of extramitochondrial ADP on permeability transition of mouse liver mitochondria. Biochim Biophys Acta 1706:98-104.

» PMID: 15620369 Open Access

Gizatullina ZZ, Chen Y, Zierz S, Gellerich FN (2005) Biochim Biophys Acta

Abstract: Carboxyatractylate (CAT) and atractylate inhibit the mitochondrial adenine nucleotide translocator (ANT) and stimulate the opening of permeability transition pore (PTP). Following pretreatment of mouse liver mitochondria with 5 μM CAT and 75 μM Ca2+, the activity of PTP increased, but addition of 2 mM ADP inhibited the swelling of mitochondria. Extramitochondrial Ca2+ concentration measured with Calcium-Green 5N evidenced that 2 mM ADP did not remarkably decrease the free Ca2+ but the release of Ca2+ from loaded mitochondria was stopped effectively after addition of 2 mM ADP. CAT caused a remarkable decrease of the maximum amount of calcium ions, which can be accumulated by mitochondria. Addition of 2 mM ADP after 5 μM CAT did not change the respiration, but increased the mitochondrial capacity for Ca2+ at more than five times. Bongkrekic acid (BA) had a biphasic effect on PT. In the first minutes 5 μM BA increased the stability of mitochondrial membrane followed by a pronounced opening of PTP too. BA abolished the action about of 1 mM ADP, but was not able to induce swelling of mitochondria in the presence of 2 mM ADP.

We conclude that the outer side of inner mitochondrial membrane has a low affinity sensor for ADP, modifying the activity of PTP. The pathophysiological importance of this process could be an endogenous prevention of PT at conditions of energetic depression. Keywords: Mitochondria, Permeability transition pore, Adenine nucleotide translocator, Carboxyatractyloside, Bongkrekic acid

O2k-Network Lab: DE Magdeburg Gellerich FN


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Organism: Mouse  Tissue;cell: Liver  Preparation: Isolated mitochondria 



HRR: Oxygraph-2k