Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Hansson 2010 J Biol Chem"

From Bioblast
Line 1: Line 1:
{{Publication
{{Publication
|title=Hansson MJ, Morota S, Teilum M, Mattiasson G, Uchino H, Elmer E (2010) Increased potassium conductance of brain mitochondria induces resistance to permeability transition by enhancing matrix volume. J Biol Chem 285: 741-750.
|title=Hansson MJ, Morota S, Teilum M, Mattiasson G, Uchino H, Elmer E (2010) Increased potassium conductance of brain mitochondria induces resistance to permeability transition by enhancing matrix volume. J Biol Chem 285: 741-750.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/19880514 PMID: 19880514]; [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2804223/?tool=pubmed PMC Text]
|info=[http://www.ncbi.nlm.nih.gov/pubmed/19880514 PMID: 19880514 Open Access]
|authors=Hansson MJ, Morota S, Teilum M, Mattiasson G, Uchino H, Elmer E
|authors=Hansson MJ, Morota S, Teilum M, Mattiasson G, Uchino H, Elmer E
|year=2010
|year=2010

Revision as of 16:21, 13 March 2013

Publications in the MiPMap
Hansson MJ, Morota S, Teilum M, Mattiasson G, Uchino H, Elmer E (2010) Increased potassium conductance of brain mitochondria induces resistance to permeability transition by enhancing matrix volume. J Biol Chem 285: 741-750.

Β» PMID: 19880514 Open Access

Hansson MJ, Morota S, Teilum M, Mattiasson G, Uchino H, Elmer E (2010) J Biol Chem

Abstract: Modulation of K+ conductance of the inner mitochondrial membrane has been proposed to mediate preconditioning in ischemia-reperfusion injury. The mechanism is not entirely understood, but it has been linked to a decreased activation of mitochondrial permeability transition (mPT). In the present study K+ channel activity was mimicked by picomolar concentrations of valinomycin. Isolated brain mitochondria were exposed to continuous infusions of calcium. Monitoring of extramitochondrial Ca2+ and mitochondrial respiration provided a quantitative assay for mPT sensitivity by determining calcium retention capacity (CRC). Valinomycin and cyclophilin D inhibition separately and additively increased CRC. Comparable degrees of respiratory uncoupling induced by increased K+ or H+ conductance had opposite effects on mPT sensitivity. Protonophores dose-dependently decreased CRC, demonstrating that so-called mild uncoupling was not beneficial per se. The putative mitoK(ATP) channel opener diazoxide did not mimic the effect of valinomycin. An alkaline matrix pH was required for mitochondria to retain calcium, but increased K+ conductance did not result in augmented DeltapH. The beneficial effect of valinomycin on CRC was not mediated by H2O2-induced protein kinase Cepsilon activation. Rather, increased K+conductance reduced H2O2 generation during calcium infusion. Lowering the osmolarity of the buffer induced an increase in mitochondrial volume and improved CRC similar to valinomycin without inducing uncoupling or otherwise affecting respiration. We propose that increased potassium conductance in brain mitochondria may cause a direct physiological effect on matrix volume inducing resistance to pathological calcium challenges. β€’ Keywords: Ischemia-reperfusion injury, K(+) channel, mitochondrial permeability transition (mPT), Isolated brain mitochondria, extramitochondrial Ca(2+); calcium retention capacity (CRC) ; Uncoupling;

β€’ O2k-Network Lab: SE_Lund_Elmer E, JP Tokyo Uchino H


Labels:

Stress:Ischemia-Reperfusion; Preservation"Ischemia-Reperfusion; Preservation" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. 

Tissue;cell: Nervous system  Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.  Enzyme: Inner mtMembrane Transporter"Inner mtMembrane Transporter" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. 

Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k